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16. Development of a flow-through [corrected] microarray based reverse transcriptase multiplex ligation-dependent probe amplification assay for the detection of European Bunyaviruses. [corrected].

Author

Hasib L, Dilcher M, Hufert F, Meyer-König U, and Weidmann M

Subjects
Animals, Arboviruses genetics, Chlorocebus aethiops, Europe, Nucleic Acid Hybridization methods, Oligonucleotides, Orthobunyavirus genetics, Phlebovirus genetics, RNA, Viral analysis, RNA, Viral isolation & purification, RNA-Directed DNA Polymerase, Sensitivity and Specificity, Temperature, Vero Cells, Arboviruses isolation & purification, Nucleic Acid Amplification Techniques methods, Oligonucleotide Array Sequence Analysis methods, Orthobunyavirus isolation & purification, Phlebovirus isolation & purification
Abstract

It is suspected that apart from tick-borne encephalitis virus several additional European Arboviruses such as the sandfly borne Toscana virus, sandfly fever Sicilian virus and sandfly fever Naples virus, mosquito-borne Tahyna virus, Inkoo virus, Batai virus and tick-borne Uukuniemi virus cause aseptic meningo-encephalitis or febrile disease in Europe. Currently, the microarray technology is developing rapidly and there are many efforts to apply it to infectious diseases diagnostics. In order to arrive at an assay system useful for high throughput analysis of samples from aseptic meningo-encephalitis cases the authors developed a combined multiplex ligation-dependent probe amplification and flow-through microarray assay for the detection of European Bunyaviruses. These results show that this combined assay indeed is highly sensitive, and specific for the accurate detection of multiple viruses.

Published
2011
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