27 results on '"Michelini, Sara"'
Search Results
2. Heterogeneity of Lipopolysaccharide as Source of Variability in Bioassays and LPS-Binding Proteins as Remedy
- Author
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Fux, Alexandra C., primary, Casonato Melo, Cristiane, additional, Michelini, Sara, additional, Swartzwelter, Benjamin J., additional, Neusch, Andreas, additional, Italiani, Paola, additional, and Himly, Martin, additional
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- 2023
- Full Text
- View/download PDF
3. Inflammatory, Oxidative Stress and Small Cellular Particle Response in HUVEC Induced by Debris from Endoprosthesis Processing
- Author
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Jan, Zala, primary, Hočevar, Matej, additional, Kononenko, Veno, additional, Michelini, Sara, additional, Repar, Neža, additional, Caf, Maja, additional, Kocjančič, Boštjan, additional, Dolinar, Drago, additional, Kralj, Slavko, additional, Makovec, Darko, additional, Iglič, Aleš, additional, Drobne, Damjana, additional, Jenko, Monika, additional, and Kralj-Iglič, Veronika, additional
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- 2023
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- View/download PDF
4. Developing a model to test microplastic impact on lung epithelial barriers formation and functionality
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Repar, Neža, Michelini, Sara, Šimunović, Katarina, Guzmán-Mínguez, Jesús C., Reinosa, Julián J., Kononenko, Veno, Mandič Mulec, Ines, Fernández, José F., and Drobne, Damjana
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inhalation exposure ,A549 ,microplastics ,lung epithelial barrier - Abstract
Nano- and microplastics (NPs/MPs) are plastic particles smaller than 5 mm. They can be manufactured for a specific purpose or released through the degradation of plastics and NP/MP-containing products, and are thus widely distributed in the environment. The two major routes of exposure for humans are ingestion through food and inhalation of indoor air. Studies have already reported that airborne particles can deposit or accumulate in the lungs causing occupational diseases such as lung cancer in textile workers. The aim of this study is to develop and test a model for the evaluation of the impact of PET and PS MPs on human health, with particular attention to the integrity and functionality of the lung barrier. To mimic lung tissue, A549 cells were grown on PET inserts, either in submerged or air-liquid interface (ALI) conditions. The latter being more similar to the natural environment in the alveoli. We then characterized our model in detail using various methods prior to MP exposure. In particular, by performing time-course experiments, we observed that transepithelial electrical resistance (TEER) and epithelial permeability (estimated by the Lucifer Yellow assay) increased and decreased, respectively, over time and reached a plateau 10 days after seeding, indicating the formation of an epithelial barrier. In subsequent experiments, we examined and compared surfactant production and tight junction expression by A549 cells grown either in submerged or ALI conditions. This showed that ALI produced more surfactant (drop spreading method) and occludin (confocal microscopy) compared to submerged cells, with no significant difference in TEER and permeability values, suggesting that ALI-grown cells represent a more realistic model for lung exposure. Finally, the exposure of A549 cells to MPs was tested using this model and our preliminary results showed no clear effect of MPs on cell viability. Also see: https://micro2022.sciencesconf.org/427123/document, In MICRO 2022, Online Atlas Edition: Plastic Pollution from MACRO to nano
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- 2022
- Full Text
- View/download PDF
5. Role of Common Cell Culture Media Supplements on Citrate-Stabilized Gold Nanoparticle Protein Corona Formation, Aggregation State, and the Consequent Impact on Cellular Uptake
- Author
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Barbero, Francesco, primary, Michelini, Sara, additional, Moriones, Oscar H., additional, Patarroyo, Javier, additional, Rosell, Jordi, additional, F. Gusta, Muriel, additional, Vitali, Michele, additional, Martín, Luna, additional, Canals, Francesc, additional, Duschl, Albert, additional, Horejs-Hoeck, Jutta, additional, Mondragón, Laura, additional, Bastús, Neus G., additional, and Puntes, Víctor, additional
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- 2022
- Full Text
- View/download PDF
6. Innate Memory Reprogramming by Gold Nanoparticles Depends on the Microbial Agents That Induce Memory
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Swartzwelter, Benjamin J., Michelini, Sara, Frauenlob, Tobias, Barbero, Francesco, Verde, Alessandro, Chiara de Luca, Anna, Puntes, Víctor F., Duschl, Albert, Horejs-Hoeck, Jutta, Italiani, Paola, Boraschi, Diana, University of Salzburg, and Austrian Science Fund
- Subjects
Innate immunity ,Staphylococcus aureus ,beta-Glucans ,Helicobacter pylori ,Innate memory ,Immunology ,Metal Nanoparticles ,Immunity, Innate ,Monocytes ,Candida albicans ,Cytokines ,Humans ,Nanoparticles ,Gold ,Microbial agents ,Immunologic Memory ,Cells, Cultured ,Original Research - Abstract
Innate immune memory, the ability of innate cells to react in a more protective way to secondary challenges, is induced by exposure to infectious and other exogeous and endogenous agents. Engineered nanoparticles are particulate exogenous agents that, as such, could trigger an inflammatory reaction in monocytes and macrophages and could therefore be also able to induce innate memory. Here, we have evaluated the capacity of engineered gold nanoparticles (AuNPs) to induce a memory response or to modulate the memory responses induced by microbial agents. Microbial agents used were in soluble vs. particulate form (MDP and the gram-positive bacteria Staphylococcus aureus; β-glucan and the β-glucan-producing fungi C. albicans), and as whole microrganisms that were either killed (S. aureus, C. albicans) or viable (the gram-negative bacteria Helicobacter pylori). The memory response was assessed in vitro, by exposing human primary monocytes from 2-7 individual donors to microbial agents with or without AuNPs (primary response), then resting them for 6 days to allow return to baseline, and eventually challenging them with LPS (secondary memory response). Primary and memory responses were tested as production of the innate/inflammatory cytokine TNFα and other inflammatory and anti-inflammatory factors. While inactive on the response induced by soluble microbial stimuli (muramyl dipeptide -MDP-, β-glucan), AuNPs partially reduced the primary response induced by whole microorganisms. AuNPs were also unable to directly induce a memory response but could modulate stimulus-induced memory in a circumscribed fashion, limited to some agents and some cytokines. Thus, the MDP-induced tolerance in terms of TNFα production was further exacerbated by co-priming with AuNPs, resulting in a less inflammatory memory response. Conversely, the H. pylori-induced tolerance was downregulated by AuNPs only relative to the anti-inflammatory cytokine IL-10, which would lead to an overall more inflammatory memory response. These effects of AuNPs may depend on a differential interaction/association between the reactive particle surfaces and the microbial components and agents, which may lead to a change in the exposure profiles. As a general observation, however, the donor-to-donor variability in memory response profiles and reactivity to AuNPs was substantial, suggesting that innate memory depends on the individual history of exposures., This work was supported by the EU Commission H2020 projects PANDORA (GA 671881) and ENDONANO (GA 812661), the Italian MIUR InterOmics Flagship projects MEMORAT and MAME, the Italian MIUR/PRIN-20173ZECCM, the Priority program ACBN (Allergy Cancer BioNano Research Centre) of the University of Salzburg, the Cancer Cluster Salzburg, the Research Grant from the University of Salzburg, and the Austrian Science Fund (FWF) Grant Nr. P 29941.
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- 2021
7. Innate Memory Reprogramming by Gold Nanoparticles Depends on the Microbial Agents That Induce Memory
- Author
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Swartzwelter, Benjamin J., primary, Michelini, Sara, additional, Frauenlob, Tobias, additional, Barbero, Francesco, additional, Verde, Alessandro, additional, De Luca, Anna Chiara, additional, Puntes, Victor, additional, Duschl, Albert, additional, Horejs-Hoeck, Jutta, additional, Italiani, Paola, additional, and Boraschi, Diana, additional
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- 2021
- Full Text
- View/download PDF
8. A combined metabolomics and peptidomics approach to discriminate anomalous rind inclusion levels in Parmigiano Reggiano PDO grated hard cheese from different ripening stages
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Rocchetti, Gabriele, primary, Michelini, Sara, additional, Pizzamiglio, Valentina, additional, Masoero, Francesco, additional, and Lucini, Luigi, additional
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- 2021
- Full Text
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9. Cross-Species Comparisons of Nanoparticle Interactions with Innate Immune Systems: A Methodological Review
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Swartzwelter, Benjamin J., primary, Mayall, Craig, additional, Alijagic, Andi, additional, Barbero, Francesco, additional, Ferrari, Eleonora, additional, Hernadi, Szabolcs, additional, Michelini, Sara, additional, Navarro Pacheco, Natividad Isabel, additional, Prinelli, Alessandra, additional, Swart, Elmer, additional, and Auguste, Manon, additional
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- 2021
- Full Text
- View/download PDF
10. Cross-species comparisons of nanoparticle interactions with innate immune systems: A methodological review
- Author
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Swartzwelter, Benjamin J., Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Auguste, Manon, and European Commission
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Innate immunity ,Markers ,NPs testing ,Environmental models ,Humen cells - Abstract
Many components of the innate immune system are evolutionarily conserved and shared across many living organisms, from plants and invertebrates to humans. Therefore, these shared features can allow the comparative study of potentially dangerous substances, such as engineered nanoparticles (NPs). However, differences of methodology and procedure between diverse species and models make comparison of innate immune responses to NPs between organisms difficult in many cases. To this aim, this review provides an overview of suitable methods and assays that can be used to measure NP immune interactions across species in a multidisciplinary approach. The first part of this review describes the main innate immune defense characteristics of the selected models that can be associated to NPs exposure. In the second part, the different modes of exposure to NPs across models (considering isolated cells or whole organisms) and the main endpoints measured are discussed. In this synergistic perspective, we provide an overview of the current state of important cross-disciplinary immunological models to study NP-immune interactions and identify future research needs. As such, this paper could be used as a methodological reference point for future nano-immunosafety studies. All authors were supported by the EU H2020 project PANDORA, grant number 671881.
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- 2021
11. Cross-species comparisons of nanoparticle interactions with innate immune systems : a methodological review
- Author
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Swartzwelter, Benjamin, Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad Isabel, Prinelli, Alessandra, Swart, Elmer, Auguste, Manon, Swartzwelter, Benjamin, Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad Isabel, Prinelli, Alessandra, Swart, Elmer, and Auguste, Manon
- Abstract
Many components of the innate immune system are evolutionarily conserved and shared across many living organisms, from plants and invertebrates to humans. Therefore, these shared features can allow the comparative study of potentially dangerous substances, such as engineered nanoparticles (NPs). However, differences of methodology and procedure between diverse species and models make comparison of innate immune responses to NPs between organisms difficult in many cases. To this aim, this review provides an overview of suitable methods and assays that can be used to measure NP immune interactions across species in a multidisciplinary approach. The first part of this review describes the main innate immune defense characteristics of the selected models that can be associated to NPs exposure. In the second part, the different modes of exposure to NPs across models (considering isolated cells or whole organisms) and the main endpoints measured are discussed. In this synergistic perspective, we provide an overview of the current state of important cross-disciplinary immunological models to study NP-immune interactions and identify future research needs. As such, this paper could be used as a methodological reference point for future nano-immunosafety studies.
- Published
- 2021
12. Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a tolerogenic-like dendritic cell phenotype with altered endosomal structures
- Author
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European Commission, Austrian Science Fund, Michelini, Sara, Barbero, Francesco, Prinelli, Alessandra, Steiner, Paul, Weiss, Richard, Verwanger, Thomas, Andosch, Ancuela, Lütz-Meindl, Ursula, Puntes, Víctor F., Drobne, Damjana, Duschl, Albert, Horejs-Hoeck, Jutta, European Commission, Austrian Science Fund, Michelini, Sara, Barbero, Francesco, Prinelli, Alessandra, Steiner, Paul, Weiss, Richard, Verwanger, Thomas, Andosch, Ancuela, Lütz-Meindl, Ursula, Puntes, Víctor F., Drobne, Damjana, Duschl, Albert, and Horejs-Hoeck, Jutta
- Abstract
Dendritic cells (DCs) shape immune responses by influencing T-cell activation. Thus, they are considered both an interesting model for studying nano-immune interactions and a promising target for nano-based biomedical applications. However, the accentuated ability of nanoparticles (NPs) to interact with biomolecules may have an impact on DC function that poses an unexpected risk of unbalanced immune reactions. Here, we investigated the potential effects of gold nanoparticles (AuNPs) on DC function and the consequences for effector and memory T-cell responses in the presence of the microbial inflammatory stimulus lipopolysaccharide (LPS). Overall, we found that, in the absence of LPS, none of the tested NPs induced a DC response. However, whereas 4-, 8-, and 11 nm AuNPs did not modulate LPS-dependent immune responses, 26 nm AuNPs shifted the phenotype of LPS-activated DCs toward a tolerogenic state, characterized by downregulation of CD86, IL-12 and IL-27, upregulation of ILT3, and induction of class E compartments. Moreover, this DC phenotype was less proficient in promoting Th1 activation and central memory T-cell proliferation. Taken together, these findings support the perception that AuNPs are safe under homeostatic conditions; however, particular care should be taken in patients experiencing a current infection or disorders of the immune system.
- Published
- 2021
13. Cross-species comparisons of nanoparticle interactions with innate immune systems: A methodological review
- Author
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European Commission, Swartzwelter, Benjamin J., Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Auguste, Manon, European Commission, Swartzwelter, Benjamin J., Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, and Auguste, Manon
- Abstract
Many components of the innate immune system are evolutionarily conserved and shared across many living organisms, from plants and invertebrates to humans. Therefore, these shared features can allow the comparative study of potentially dangerous substances, such as engineered nanoparticles (NPs). However, differences of methodology and procedure between diverse species and models make comparison of innate immune responses to NPs between organisms difficult in many cases. To this aim, this review provides an overview of suitable methods and assays that can be used to measure NP immune interactions across species in a multidisciplinary approach. The first part of this review describes the main innate immune defense characteristics of the selected models that can be associated to NPs exposure. In the second part, the different modes of exposure to NPs across models (considering isolated cells or whole organisms) and the main endpoints measured are discussed. In this synergistic perspective, we provide an overview of the current state of important cross-disciplinary immunological models to study NP-immune interactions and identify future research needs. As such, this paper could be used as a methodological reference point for future nano-immunosafety studies.
- Published
- 2021
14. In silico Design of Phl p 6 Variants With Altered Fold-Stability Significantly Impacts Antigen Processing, Immunogenicity and Immune Polarization
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Winter, Petra, Stubenvoll, Stefan, Scheiblhofer, Sandra, Joubert, Isabella A., Strasser, Lisa, Briganser, Carolin, Soh, Wai Tuck, Hofer, Florian, Kamenik, Anna Sophia, Dietrich, Valentin, Michelini, Sara, Laimer, Josef, Lackner, Peter, Horejs-Hoeck, Jutta, Tollinger, Martin, Liedl, Klaus R., Brandstetter, Johann, Huber, Christian G., and Weiss, Richard
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lcsh:Immunologic diseases. Allergy ,in silico mutant screening ,Immunology ,structural stability ,Immunology and Allergy ,immune polarization ,antigen processing and presentation ,in silico mutagenesis ,protein stabilization ,lcsh:RC581-607 ,Original Research ,endolysosomal degradation - Abstract
Introduction:Understanding, which factors determine the immunogenicity and immune polarizing properties of proteins, is an important prerequisite for designing better vaccines and immunotherapeutics. While extrinsic immune modulatory factors such as pathogen associated molecular patterns are well-understood, far less is known about the contribution of protein inherent features. Protein fold-stability represents such an intrinsic feature contributing to immunogenicity and immune polarization by influencing the amount of peptide-MHC II complexes (pMHCII). Here, we investigated how modulation of the fold-stability of the grass pollen allergen Phl p 6 affects its ability to stimulate immune responses and T cell polarization. Methods:MAESTRO software was used forin silicoprediction of stabilizing or destabilizing point mutations. Mutated proteins were expressed inE. coli, and their thermal stability and resistance to endolysosomal proteases was determined. Resulting peptides were analyzed by mass spectrometry. The structure of the most stable mutant protein was assessed by X-ray crystallography. We evaluated the capacity of the mutants to stimulate T cell proliferationin vitro, as well as antibody responses and T cell polarizationin vivoin an adjuvant-free BALB/c mouse model. Results:In comparison to wild-type protein, stabilized or destabilized mutants displayed changes in thermal stability ranging from -5 to +14 degrees. While highly stabilized mutants were degraded very slowly, destabilization led to faster proteolytic processingin vitro. This was confirmed in BMDCs, which processed and presented the immunodominant epitope from a destabilized mutant more efficiently compared to a highly stable mutant.In vivo, stabilization resulted in a shift in immune polarization from TH2 to TH1/TH17 as indicated by higher levels of IgG2a and increased secretion of TNF-alpha, IFN-gamma, IL-17, and IL-21. Conclusion:MAESTRO software was very efficient in detecting single point mutations that increase or reduce fold-stability. Thermal stability correlated well with the speed of proteolytic degradation and presentation of peptides on the surface of dendritic cellsin vitro. This change in processing kinetics significantly influenced the polarization of T cell responsesin vivo. Modulating the fold-stability of proteins thus has the potential to optimize and polarize immune responses, which opens the door to more efficient design of molecular vaccines.
- Published
- 2020
15. Frontiers in Immunology / Mechanisms of particles in sensitization, effector function and therapy of allergic disease
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Joubert, Anna I., Geppert, Mark, Johnson, Litty, Mills-Goodlet, Robert, Michelini, Sara, Korotchenko, Evgeniia, Duschl, Albert, Weiss, Richard, Horejs-Höck, Jutta, and Himly, Martin
- Subjects
alum ,adjuvants ,animal dander ,pollen ,house dust mite feces ,immunomodulation ,nanomedicine ,mold spores - Abstract
Humans have always been in contact with natural airborne particles from many sources including biologic particulate matter (PM) which can exhibit allergenic properties. With industrialization, anthropogenic and combustion-derived particles have become a major fraction. Currently, an ever-growing number of diverse and innovative materials containing engineered nanoparticles (NPs) are being developed with great expectations in technology and medicine. Nanomaterials have entered everyday products including cosmetics, textiles, electronics, sports equipment, as well as food, and food packaging. As part of natural evolution humans have adapted to the exposure to particulate matter, aiming to protect the individual's integrity and health. At the respiratory barrier, complications can arise, when allergic sensitization and pulmonary diseases occur in response to particle exposure. Particulate matter in the form of plant pollen, dust mites feces, animal dander, but also aerosols arising from industrial processes in occupational settings including diverse mixtures thereof can exert such effects. This review article gives an overview of the allergic immune response and addresses specifically the mechanisms of particulates in the context of allergic sensitization, effector function and therapy. In regard of the first theme (i), an overview on exposure to particulates and the functionalities of the relevant immune cells involved in allergic sensitization as well as their interactions in innate and adaptive responses are described. As relevant for human disease, we aim to outline (ii) the potential effector mechanisms that lead to the aggravation of an ongoing immune deviation (such as asthma, chronic obstructive pulmonary disease, etc.) by inhaled particulates, including NPs. Even though adverse effects can be exerted by (nano)particles, leading to allergic sensitization, and the exacerbation of allergic symptoms, promising potential has been shown for their use in (iii) therapeutic approaches of allergic disease, for example as adjuvants. Hence, allergen-specific immunotherapy (AIT) is introduced and the role of adjuvants such as alum as well as the current understanding of their mechanisms of action is reviewed. Finally, future prospects of nanomedicines in allergy treatment are described, which involve modern platform technologies combining immunomodulatory effects at several (immuno-)functional levels. (VLID)5337718
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- 2020
16. Frontiers in Immunology / In silico Design of Phl p 6 variants with altered fold-stability significantly impacts antigen processing, immunogenicity and immune polarization
- Author
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Winter, Petra, Stubenvoll, Stefan, Scheiblhofer, Sandra, Joubert, Isabella A., Strasser, Lisa, Briganser, Carolin, Soh, Wai Tuck, Hofer, Florian, Kamenik, Anna Sophia, Dietrich, Valentin, Michelini, Sara, Laimer, Josef, Lackner, Peter, Horejs-Hoeck, Jutta, Tollinger, Martin, Liedl, Klaus R., Brandstetter, Johann, Huber, Christian G., and Weiss, Richard
- Subjects
in silico mutant screening ,structural stability ,immune polarization ,antigen processing and presentation ,in silico mutagenesis ,protein stabilization ,endolysosomal degradation - Abstract
Introduction: Understanding, which factors determine the immunogenicity and immune polarizing properties of proteins, is an important prerequisite for designing better vaccines and immunotherapeutics. While extrinsic immune modulatory factors such as pathogen associated molecular patterns are well-understood, far less is known about the contribution of protein inherent features. Protein fold-stability represents such an intrinsic feature contributing to immunogenicity and immune polarization by influencing the amount of peptide-MHC II complexes (pMHCII). Here, we investigated how modulation of the fold-stability of the grass pollen allergen Phl p 6 affects its ability to stimulate immune responses and T cell polarization. Methods: MAESTRO software was used for in silico prediction of stabilizing or destabilizing point mutations. Mutated proteins were expressed in E. coli, and their thermal stability and resistance to endolysosomal proteases was determined. Resulting peptides were analyzed by mass spectrometry. The structure of the most stable mutant protein was assessed by X-ray crystallography. We evaluated the capacity of the mutants to stimulate T cell proliferation in vitro, as well as antibody responses and T cell polarization in vivo in an adjuvant-free BALB/c mouse model. Results: In comparison to wild-type protein, stabilized or destabilized mutants displayed changes in thermal stability ranging from 5 to +14. While highly stabilized mutants were degraded very slowly, destabilization led to faster proteolytic processing in vitro. This was confirmed in BMDCs, which processed and presented the immunodominant epitope from a destabilized mutant more efficiently compared to a highly stable mutant. In vivo, stabilization resulted in a shift in immune polarization from TH2 to TH1/TH17 as indicated by higher levels of IgG2a and increased secretion of TNF-, IFN-, IL-17, and IL-21. Conclusion: MAESTRO software was very efficient in detecting single point mutations that increase or reduce fold-stability. Thermal stability correlated well with the speed of proteolytic degradation and presentation of peptides on the surface of dendritic cells in vitro. This change in processing kinetics significantly influenced the polarization of T cell responses in vivo. Modulating the fold-stability of proteins thus has the potential to optimize and polarize immune responses, which opens the door to more efficient design of molecular vaccines. (VLID)5400402
- Published
- 2020
17. Addressing nanomaterial immunosafety by evaluating innate immunity across living species
- Author
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Boraschi, Diana, Alijagic, Andi, Auguste, Manon, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Mayall, Craig, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Swartzwelter, Benjamin J., Bastús, Neus G., Canesi, Laura, Drobne, Damjana, Duschl, Albert, Ewart, Marie‐Ann, Horejs‐Hoeck, Jutta, Italiani, Paola, Kemmerling, Birgit, Kille, Peter, Prochazkova, Petra, Puntes, Victor F., Spurgeon, David J., Svendsen, Claus, Wilde, Colin J., Pinsino, Annalisa, Boraschi, Diana, Alijagic, Andi, Auguste, Manon, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Mayall, Craig, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Swartzwelter, Benjamin J., Bastús, Neus G., Canesi, Laura, Drobne, Damjana, Duschl, Albert, Ewart, Marie‐Ann, Horejs‐Hoeck, Jutta, Italiani, Paola, Kemmerling, Birgit, Kille, Peter, Prochazkova, Petra, Puntes, Victor F., Spurgeon, David J., Svendsen, Claus, Wilde, Colin J., and Pinsino, Annalisa
- Abstract
The interaction of a living organism with external foreign agents is a central issue for its survival and adaptation to the environment. Nanosafety should be considered within this perspective, and it should be examined that how different organisms interact with engineered nanomaterials (NM) by either mounting a defensive response or by physiologically adapting to them. Herein, the interaction of NM with one of the major biological systems deputed to recognition of and response to foreign challenges, i.e., the immune system, is specifically addressed. The main focus is innate immunity, the only type of immunity in plants, invertebrates, and lower vertebrates, and that coexists with adaptive immunity in higher vertebrates. Because of their presence in the majority of eukaryotic living organisms, innate immune responses can be viewed in a comparative context. In the majority of cases, the interaction of NM with living organisms results in innate immune reactions that eliminate the possible danger with mechanisms that do not lead to damage. While in some cases such interaction may lead to pathological consequences, in some other cases beneficial effects can be identified.
- Published
- 2020
18. Addressing nanomaterial immunosafety by evaluating innate immunity across living species
- Author
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Ministero dell'Istruzione, dell'Università e della Ricerca, Austrian Science Fund, Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Ministry of Education, Youth and Sports (Czech Republic), Boraschi, Diana, Alijagic, Andi, Auguste, Manon, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Mayall, Craig, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Swartzwelter, Benjamin J., Bastús, Neus G., Canesi, Laura, Drobne, Damjana, Duschl, Albert, Ewart, Marie-Ann, Horejs-Hoeck, Jutta, Italiani, Paola, Kemmerling, Birgit, Kille, Peter, Prochazkova, Petra, Puntes, Víctor F., Spurgeon, David J., Svendsen, Claus, Wilde, Colin J., Pinsino, Annalisa, Ministero dell'Istruzione, dell'Università e della Ricerca, Austrian Science Fund, Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Ministry of Education, Youth and Sports (Czech Republic), Boraschi, Diana, Alijagic, Andi, Auguste, Manon, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Mayall, Craig, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Swartzwelter, Benjamin J., Bastús, Neus G., Canesi, Laura, Drobne, Damjana, Duschl, Albert, Ewart, Marie-Ann, Horejs-Hoeck, Jutta, Italiani, Paola, Kemmerling, Birgit, Kille, Peter, Prochazkova, Petra, Puntes, Víctor F., Spurgeon, David J., Svendsen, Claus, Wilde, Colin J., and Pinsino, Annalisa
- Abstract
The interaction of a living organism with external foreign agents is a central issue for its survival and adaptation to the environment. Nanosafety should be considered within this perspective, and it should be examined that how different organisms interact with engineered nanomaterials (NM) by either mounting a defensive response or by physiologically adapting to them. Herein, the interaction of NM with one of the major biological systems deputed to recognition of and response to foreign challenges, i.e., the immune system, is specifically addressed. The main focus is innate immunity, the only type of immunity in plants, invertebrates, and lower vertebrates, and that coexists with adaptive immunity in higher vertebrates. Because of their presence in the majority of eukaryotic living organisms, innate immune responses can be viewed in a comparative context. In the majority of cases, the interaction of NM with living organisms results in innate immune reactions that eliminate the possible danger with mechanisms that do not lead to damage. While in some cases such interaction may lead to pathological consequences, in some other cases beneficial effects can be identified.
- Published
- 2020
19. IL-1β Induces SOCS2 Expression in Human Dendritic Cells
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Sarajlic, Muamera, Neuper, Theresa, Föhrenbach Quiroz, Kim Tamara, Michelini, Sara, Vetter, Julia, Schaller, Susanne, and Horejs-Hoeck, Jutta
- Subjects
dendritic cell ,Communication ,Interleukin-1beta ,Cell Differentiation ,Suppressor of Cytokine Signaling Proteins ,Dendritic Cells ,Lymphocyte Activation ,Monocytes ,IL-1β ,Cytokines ,Humans ,SOCS2 ,Cells, Cultured ,Signal Transduction - Abstract
Dendritic cells (DCs) regulate immunity and inflammation and respond to various stimuli, including cytokines. IL-1β is a key cytokine in the course of both acute and chronic inflammatory responses, making it indispensable for protection of the host, but also linking it to several diseases. Thus, IL-1β signaling must be tightly regulated. As suppressor of cytokine signaling (SOCS) proteins effectively control immune responses, we investigated the role of SOCS2 in IL-1β-induced DC activation. Human monocyte-derived DCs were stimulated with IL-1β, and SOCS2 mRNA and protein levels were measured. DC activation was assessed by cytokine secretion and surface marker expression. For functional analysis, small interfering RNA (siRNA)-based SOCS2 silencing was performed. SOCS2 expression was also analyzed in a curated NCBI GEO dataset of myeloid leukemia patients. We found IL-1β to be a potent inducer of SOCS2 expression. By silencing SOCS2, we showed that SOCS2 specifically limits IL-1β-induced IL-8 secretion. Moreover, our analysis revealed that SOCS2 levels are significantly increased in patients with acute and chronic myeloid leukemia, two hematological malignancies where disease progression is closely linked to IL-1β. This study identifies SOCS2 as a novel IL-1β-inducible target gene and points toward a potential role of SOCS2 in IL-1β-mediated DC activation.
- Published
- 2019
20. Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a tolerogenic-like dendritic cell phenotype with altered endosomal structures
- Author
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Michelini, Sara, primary, Barbero, Francesco, additional, Prinelli, Alessandra, additional, Steiner, Philip, additional, Weiss, Richard, additional, Verwanger, Thomas, additional, Andosch, Ancuela, additional, Lütz-Meindl, Ursula, additional, Puntes, Victor F., additional, Drobne, Damjana, additional, Duschl, Albert, additional, and Horejs-Hoeck, Jutta, additional
- Published
- 2021
- Full Text
- View/download PDF
21. Preliminary Assessment of Parmigiano Reggiano Authenticity by Handheld Raman Spectroscopy
- Author
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Li Vigni, Mario, primary, Durante, Caterina, additional, Michelini, Sara, additional, Nocetti, Marco, additional, and Cocchi, Marina, additional
- Published
- 2020
- Full Text
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22. Mechanisms of Particles in Sensitization, Effector Function and Therapy of Allergic Disease
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Joubert, Isabella Anna, primary, Geppert, Mark, additional, Johnson, Litty, additional, Mills-Goodlet, Robert, additional, Michelini, Sara, additional, Korotchenko, Evgeniia, additional, Duschl, Albert, additional, Weiss, Richard, additional, Horejs-Höck, Jutta, additional, and Himly, Martin, additional
- Published
- 2020
- Full Text
- View/download PDF
23. Addressing Nanomaterial Immunosafety by Evaluating Innate Immunity across Living Species
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Boraschi, Diana, primary, Alijagic, Andi, additional, Auguste, Manon, additional, Barbero, Francesco, additional, Ferrari, Eleonora, additional, Hernadi, Szabolcs, additional, Mayall, Craig, additional, Michelini, Sara, additional, Navarro Pacheco, Natividad I., additional, Prinelli, Alessandra, additional, Swart, Elmer, additional, Swartzwelter, Benjamin J., additional, Bastús, Neus G., additional, Canesi, Laura, additional, Drobne, Damjana, additional, Duschl, Albert, additional, Ewart, Marie‐Ann, additional, Horejs‐Hoeck, Jutta, additional, Italiani, Paola, additional, Kemmerling, Birgit, additional, Kille, Peter, additional, Prochazkova, Petra, additional, Puntes, Victor F., additional, Spurgeon, David J., additional, Svendsen, Claus, additional, Wilde, Colin J., additional, and Pinsino, Annalisa, additional
- Published
- 2020
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24. In silico design of Phl p 6 variants with altered folding stability significantly impacts antigen processing, immunogenicity and immune polarization
- Author
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Winter, Petra, primary, Stubenvoll, Stefan, additional, Scheiblhofer, Sandra, additional, Joubert, Isabella A, additional, Strasser, Lisa, additional, Briganser, Carolin, additional, Tuck, Soh Wai, additional, Hofer, Florian, additional, Kamenik, Anna Sophia, additional, Dietrich, Valentin, additional, Michelini, Sara, additional, Laimer, Josef, additional, Lackner, Peter, additional, Horejs-Hoeck, Jutta, additional, Tollinger, Martin, additional, Liedl, Klaus R., additional, Brandstetter, Johann, additional, Huber, Christian G., additional, and Richard, Weiss, additional
- Published
- 2020
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25. International Journal of Molecular Sciences / IL-1 induces SOCS2 expression in human dendritic cells
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Sarajlic, Muamera, Neuper, Theresa, Föhrenbach Quiroz, Kim Tamara, Michelini, Sara, Vetter, Julia, Schaller, Susanne, and Horejs-Hoeck, Jutta
- Subjects
dendritic cell ,IL-1 ,SOCS2 - Abstract
Dendritic cells (DCs) regulate immunity and inflammation and respond to various stimuli, including cytokines. IL-1 is a key cytokine in the course of both acute and chronic inflammatory responses, making it indispensable for protection of the host, but also linking it to several diseases. Thus, IL-1 signaling must be tightly regulated. As suppressor of cytokine signaling (SOCS) proteins effectively control immune responses, we investigated the role of SOCS2 in IL-1-induced DC activation. Human monocyte-derived DCs were stimulated with IL-1, and SOCS2 mRNA and protein levels were measured. DC activation was assessed by cytokine secretion and surface marker expression. For functional analysis, small interfering RNA (siRNA)-based SOCS2 silencing was performed. SOCS2 expression was also analyzed in a curated NCBI GEO dataset of myeloid leukemia patients. We found IL-1 to be a potent inducer of SOCS2 expression. By silencing SOCS2, we showed that SOCS2 specifically limits IL-1-induced IL-8 secretion. Moreover, our analysis revealed that SOCS2 levels are significantly increased in patients with acute and chronic myeloid leukemia, two hematological malignancies where disease progression is closely linked to IL-1. This study identifies SOCS2 as a novel IL-1-inducible target gene and points toward a potential role of SOCS2 in IL-1-mediated DC activation. (VLID)4602967
- Published
- 2019
26. IL-1β induces expression of costimulatory molecules and cytokines but not immune feedback regulators in dendritic cells
- Author
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Michelini, Sara, Sarajlic, Muamera, Duschl, Albert, and Horejs-Hoeck, Jutta
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- 2018
- Full Text
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27. A model for interfacial electron transfer on colloidal melanin
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Crippa, Pier Raimondo and Michelini, Sara
- Published
- 1999
- Full Text
- View/download PDF
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