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Your search keyword '"Pitkin, John W."' showing total 12 results
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12 results on '"Pitkin, John W."'

5. Targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases

Author

Scott-Craig, John S., Cheng, Yi-Qiang, Cervone, Felice, De Lorenzo, Giulia, Pitkin, John W., and Walton, Jonathan D.

Subjects
Pectin -- Research, Microbial mutation -- Research, Microbial enzymes -- Research, Biological sciences
Abstract

Research was conducted to examine the targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases (exoPG). The purification of the exoPG activity of C carbonum and the cloning of its gene, PGX1, was also performed. Double pgn1/pgx1 mutant strain was developed by crossing. The continued growth of the pgn1/pgx1 mutant strain on pectin could be attributed to one or more of PG residual activities.

Published
1998

6. A putative cyclic peptide efflux pump encoded by the TOXA gene of the plant-pathogenic fungus Cochliobolus carbonum

Author

Pitkin, John W., Panaccione, Daniel G., and Walton, Jonathan D.

Subjects
Fungi -- Research, Microbial toxins -- Research, Amino acid sequence -- Analysis, Biological sciences
Abstract

The TOXA gene of the plant-pathogen fungus, Cochliobolus carbonum, encodes an HC-toxin efflux pump which plays a role in self-protection against HC-toxin. The gene is present only in the fungal isolates that make HC-toxin, and it occurs as two linked copies. TOXA occurs close to HTS1, the gene found in HC-toxin making fungal isolates, that codes for the enzyme necessary for the synthesis of HC-toxin. The amino acid sequence of TOXA gene is discussed.

Published
1996

12. Targeted mutants of Cochliobolus carbonum lacking the two major extracelluar polygalacturonases.

Author

Pitkin, John W., Walton, Jonathan D., Scott-Craig, John S., Cheng, Yi-Qiang, Cervone, Felice, and De Lorenzo, Giulia

Subjects
*BIODEGRADATION, *PECTINS, *PATHOGENIC bacteria
Abstract

Presents a study to address the requirement for any pectin-degrading ability in pathogenesis by Cochliobolus carbonum. Methodology used to conduct study; Indication of findings; Discussion on results.

Published
1998
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