Search

Your search keyword '"S∅rensen, S∅ren J."' showing total 4 results
Start Over Author "S∅rensen, S∅ren J." Publication Year Range Last 50 years
4 results on '"S∅rensen, S∅ren J."'

1. Additional file 1 of The treasure inside barley seeds: microbial diversity and plant beneficial bacteria

Author

Bziuk, Nina, Maccario, Lorrie, Straube, Benjamin, Wehner, Gwendolin, S��rensen, S��ren J., Schikora, Adam, and Smalla, Kornelia

Subjects
food and beverages
Abstract

Additional file 1. Table S1. Primer sequences. Table S2. Pielou���s evenness, Species richness and Shannon index of endophytic seed microbial communities obtained from seven different barley genotypes. Table S3. Dominant phyla in the endophytic seed microbiome of seven different barley genotypes. Table S4. Pielou���s evenness, Species richness and Shannon index of bulk soil and barley rhizosphere samples from seven different genotypes grown in mouldboard plough (MP) soil. Table S5. Pielou���s evenness, Species richness and Shannon index of bulk soil and barley rhizosphere samples from seven different genotypes grown in cultivator tillage (CT) soil. Table S6. PERMANOVA of each barley genotype of rhizosphere samples obtained from plants grown in mouldboard plough (MP) soil. Table S7. PERMANOVA of each versus each barley genotype of rhizosphere samples obtained from plants grown in cultivator tillage (CT) soil. Table S8. Dominant phyla in bulk soil and barley rhizosphere of seven different genotypes grown in two diverse soils (MP; CT). Table S9. Twenty most abundant genera in bulk soil and barley rhizosphere of seven different genotypes grown in two diverse soils (MP; CT). Figure S1. Rarefaction curves of 16S rRNA gene amplicon sequencing data of the barley seed microbiome. Figure S2. Microbial diversity of the endophytic seed microbiome varied between six barley genotypes originated from the same place. The microbial alpha-diversity indices Species richness (A) and Shannon diversity index (B) varied depending on the plant genotype (ANOVA for Species richness p ��� 0.001 and Shannon index p ��� 0.01). Beta-diversity of the endophytic seed microbiome was visualized by NMDS (C). The microbiome composition based on Bray-Curtis community dissimilarities (ASVs obtained from 16S rRNA gene amplicon sequencing) and was assessed from DNA of surface-sterilized barley seeds of six genotypes harvested at the same field site. Figure S3. Logarithm of colony forming units (CFUs)/g seed of seven different barley genotypes determined after 48 h incubation at 28 ��C. 0.5 g of surface-sterilized seeds were ground and solved in 4.5 mL sterile double-distilled water. The seed suspension was plated on R2A supplemented with 100 ��g/mL cycloheximide. Figure S4. BOX fingerprint of isolated endophytes taxonomically affiliated to the genus Curtobacterium. Figure S5. BOX fingerprint of isolated endophytes taxonomically affiliated to the genus Paenibacillus. Figure S6. Rarefaction curves of 16S rRNA gene amplicon sequencing data of barley rhizosphere and bulk soil samples from two different soils (MP: mouldboard plough; CT: cultivator tillage) and seven different genotypes. Figure S7. Shannon index of bulk soil and barley rhizosphere samples of seven different genotypes grown in MP and CT soils. Figure S8. Non-metric multidimensional scaling (NMDS) of barley rhizosphere microbial communities from seven different genotypes grown in MP (A) and CT (B) soil. The rhizosphere community composition based on Bray-Curtis dissimilarities and was obtained from ASVs. The seven different genotypes were grown in MP (mouldboard plough) and CT (cultivator tillage) soil until BBCH13. Respective bulk soil samples are not shown. ANOSIM verified significant differences between the genotypes (p ��� 0.001)

Published
2021
Full Text
View/download PDF

3. Physiological heterogeneities in microbial populations and implications for physical stress tolerance.

Author

Carlquist, Magnus, Lencastre Fernandes, Rita, Helmark, S›ren, Heins, Anna-Lena, Lundin, Luisa, S›rensen, S›ren J., Gernaey, Krist V., and Eliasson Lantz, Anna

Subjects
STRESS tolerance (Psychology), PSYCHOLOGICAL stress, BACTERIA, GREEN fluorescent protein, FLUORESCENT polymers, PROTEINS
Abstract

Background: Traditionally average values of the whole population are considered when analysing microbial cell cultivations. However, a typical microbial population in a bioreactor is heterogeneous in most phenotypes measurable at a single-cell level. There are indications that such heterogeneity may be unfavourable on the one hand (reduces yields and productivities), but also beneficial on the other hand (facilitates quick adaptation to new conditions--i.e. increases the robustness of the fermentation process). Understanding and control of microbial population heterogeneity is thus of major importance for improving microbial cell factory processes. Results: In this work, a dual reporter system was developed and applied to map growth and cell fitness heterogeneities within budding yeast populations during aerobic cultivation in well-mixed bioreactors. The reporter strain, which was based on the expression of green fluorescent protein (GFP) under the control of the ribosomal protein RPL22a promoter, made it possible to distinguish cell growth phases by the level of fluorescence intensity. Furthermore, by exploiting the strong correlation of intracellular GFP level and cell membrane integrity it was possible to distinguish subpopulations with high and low cell membrane robustness and hence ability to withstand freeze-thaw stress. A strong inverse correlation between growth and cell membrane robustness was observed, which further supports the hypothesis that cellular resources are limited and need to be distributed as a trade-off between two functions: growth and robustness. In addition, the trade-off was shown to vary within the population, and the occurrence of two distinct subpopulations shifting between these two antagonistic modes of cell operation could be distinguished. Conclusions: The reporter strain enabled mapping of population heterogeneities in growth and cell membrane robustness towards freeze-thaw stress at different phases of cell cultivation. The described reporter system is a valuable tool for understanding the effect of environmental conditions on population heterogeneity of microbial cells and thereby to understand cell responses during industrial process-like conditions. It may be applied to identify more robust subpopulations, and for developing novel strategies for strain improvement and process design for more effective bioprocessing. [ABSTRACT FROM AUTHOR]

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results