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429 results on '"Sbrana F"'

1. Reconstructing the free energy landscape of a polyprotein by single-molecule experiments

Author

Imparato, A., Sbrana, F., and Vassalli, M.

Subjects
Condensed Matter - Soft Condensed Matter, Condensed Matter - Statistical Mechanics, Quantitative Biology - Biomolecules
Abstract

The mechanical unfolding of an engineered protein composed of eight domains of Ig27 is investigated by using atomic force microscopy. Exploiting a fluctuation relation, the equilibrium free energy as a function of the molecule elongation is estimated from pulling experiments. Such a free energy exhibits a regular shape that sets a typical unfolding length at zero force of the order of 20 nm. This length scale turns out to be much larger than the kinetic unfolding length that is also estimated by analyzing the typical rupture force of the molecule under dynamic loading.

Published
2008
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6. PCSK9 Inhibitors’ New Users: Analysis of Prescription Patterns and Patients’ Characteristics from an Italian Real-world Study

Author

Piccinni, C, Antonazzo, I, Maggioni, A, Pedrini, A, Calabria, S, Ronconi, G, Dondi, L, Martini, N, Roberto, G, Sampietro, T, Sbrana, F, Pino, B, Bigazzi, F, Surdo, G, Volpi, E, Biagini, S, Gini, R, Piccinni C, Antonazzo IC, Maggioni AP, Pedrini A, Calabria S, Ronconi G, Dondi L, Martini N, Roberto G, Sampietro T, Sbrana F, Pino BD, Bigazzi F, Surdo GL, Volpi E, Biagini S, Gini R, Piccinni, C, Antonazzo, I, Maggioni, A, Pedrini, A, Calabria, S, Ronconi, G, Dondi, L, Martini, N, Roberto, G, Sampietro, T, Sbrana, F, Pino, B, Bigazzi, F, Surdo, G, Volpi, E, Biagini, S, Gini, R, Piccinni C, Antonazzo IC, Maggioni AP, Pedrini A, Calabria S, Ronconi G, Dondi L, Martini N, Roberto G, Sampietro T, Sbrana F, Pino BD, Bigazzi F, Surdo GL, Volpi E, Biagini S, and Gini R

Abstract

BACKGROUND AND OBJECTIVE: Cardiovascular (CV) diseases represent a major cause of death and severe medical condition worldwide. Different therapeutic options are available to control low-density lipoprotein cholesterol (LDL-C) level in order to prevent CV events. In recent years, two new drugs were approved for patients who are unable to reduce circulating LDL-C with the current therapies: evolocumab and alirocumab (proprotein convertase subtilisin/kexin type nine [PCSK9] inhibitors). This study was aimed to characterise patients who started treatment with PCSK9 inhibitors in the Tuscany region of Italy during the first year of public healthcare service reimbursement and to describe the pattern of PCSK9 inhibitor use in the first 6 months of treatment. METHODS: Patients on PCSK9 inhibitor treatment in Tuscany (3.7 million inhabitants) from 07/2017 to 06/2018 were selected from regional healthcare administrative databases. Concomitant use of lipid-lowering therapies (LLTs), adherence and persistence during the 6 months preceding the first PCSK9 inhibitor dispensing, as well as comorbidities since 1996, were described. In the first 6 months of PCSK9 inhibitor treatment, adherence, persistence and concomitant LLTs were assessed. RESULTS: There were 269 (176 evolocumab, 93 alirocumab) new users of PCSK9 inhibitors. Patients (mean age of 59.1 years) were mainly male (71.0%) in secondary prevention (70.2%) and affected by familial hypercholesterolaemia (53.5%). Sixty-six patients (24.5%) had diabetes mellitus and 12 (4.5%) chronic renal failure. In the 6 months prior to the first PCSK9 inhibitor administration, 61.3% of patients received at least one prescription of ezetimibe or high-intensity statins and 45.7% were persistent to these drugs. During follow-up, 79.9% of patients were adherent to PCSK9 inhibitor and 73.3% were persistent. CONCLUSIONS: During the first year of availability, the rate of prescription of PCSK9 inhibitors appears below expectations. Patients wer

Published
2020

8. Approach to patients with COVID-19 disease: The procedure in Udine

Author

Agostinis, P., Bontempo, G., Della Siega, P., Gerussi, V., Pagotto, A., Barbano, E., Mazzoran, L., Calci, M., Sponza, M., Sbrana, F., Fapranzi, S., Baritussio, A., and Carlo Tascini

Subjects
Chest CT, Lung ultrasound, SARS-CoV-2, ARDS, Infection control, Outbreak, COVID-19 disease
Published
2021

17. Light emitting porous silicon diode based on a silicon/porous silicon heterojunction

Author

Pavesi, L., Chierchia, R., Bellutti, P., Lui, A., Fuso, F., Labardi, M., Pardi, L., Sbrana, F., Allegrini, M., Trusso, S., Vasi, C., Ventura, P.J., Costa, L.C., Carmo, M.C., and Bisi, O.

Subjects
Light-emitting diodes -- Research, Optoelectronics -- Research, Quantum electronics -- Research, Physics
Abstract

A new structure is presented for improving the external quantum efficiency of porous silicon light emitting diodes. The structure is based on a silicon/porous silicon heterojunction.

Published
1999

25. Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea

Author

Penna, Antonella, Casabianca, Silvia, Sbrana, F., Gjeci, Nikolla, Capellacci, Samuela, Asnaghi, Valentina, Ottaviani, Ennio, Giussani, Valentina, Pugliese, L., Jauzein, Cécile, Lemée, Rodolphe, Hachani, M.A., Turki, S., Acaf, L., Abboud-Abi Saab, Marie, Fricke, Anna, Mangialajo, L., Bertolotto, R., Totti, Cecilia, Accoroni, Stefano, Berdalet, Elisa, Vila, Magda, Chiantore, Maria Chiara, Vassalli, M., Penna, Antonella, Casabianca, Silvia, Sbrana, F., Gjeci, Nikolla, Capellacci, Samuela, Asnaghi, Valentina, Ottaviani, Ennio, Giussani, Valentina, Pugliese, L., Jauzein, Cécile, Lemée, Rodolphe, Hachani, M.A., Turki, S., Acaf, L., Abboud-Abi Saab, Marie, Fricke, Anna, Mangialajo, L., Bertolotto, R., Totti, Cecilia, Accoroni, Stefano, Berdalet, Elisa, Vila, Magda, Chiantore, Maria Chiara, and Vassalli, M.

Abstract

This study describes the adoption and validation of two innovative methods for the automated count of Ostreopsis spp. concentration in sea water: a molecular assay based on qPCR and an opto-electronic device allowing to automatically acquire images from the whole sample and recognize the target algae on the basis of a machine learning algorithm. The two approaches were tested on samples coming from different locations in 5 countries along the Mediterranean Sea, collected in the framework of the cross-border EU ENPI CBCMED Project M3-HABs, and compared with the standard visual counting method. The results demonstrate the effectiveness of both automatic approaches which provide a valuable tool, mostly cost and time effective, for the establishment of large-scale and effective monitoring of Ostreopsis spp. blooms. Moreover, the two automatic methods demonstrated the ability to discriminate for the presence of a different but similar species, O. fattorussoi, for which new species-specific qPCR primers were developed

Published
2018

28. Surgical management of acute pancreatitis in Italy: lessons from a prospective multicentre study

Author

De Rai, Paolo, Zerbi, Alessandro, Castoldi, Laura, Bassi, Claudio, Frulloni, Luca, Uomo, Generoso, Gabbrielli, Armando, Pezzilli, Raffaele, Cavallini, Giorgio, Di Carlo, Valerio, Agugiaro, S., Turri, L., Bartoli, A., Barberini, F., Cavazzoni, G., Bartolo, F., Della Papa, D., Bassi, N., Massani, M., Benedetti, A., Macarri, G., Piergallini, L., Briani, G., Bartolasi, L., Bugnano, L., Buonanno, G. M., Esposito, C., Cordovana, A., Cavina, E., Seccia, M., Lippolis, P., Musco, B., Barletta, M., Chilovi, E., De Guelfi, A., Chirletti, P., Caronna, R., Scozzafava, S., Cardi, M., Cirino, E., Buffone, A., Colangelo, E., Caracino, V., Cortese, F., Casentini, A., Costamagna, G., Trincali, A., Curzio, M., Clivio, S., Segato, S., D'Alessandro, A., Ambrosiani, V., D'Ambrosio, B., Chiodo, C., Dicillo, M., Reale, L., Grandolfo, A., Fabbrucci, P., Bruscino, A., Mugnaini, P., Ferrarese, S., Ugenti, I., Forte, G. B., Rocco, P., Franzè, A., Bertelè, A., Sereni, G., Friedman, Daniele, Mariani, L., Morelli, F., Gai, V., Antro, C., Garcea, D., Gardini, A., Lucci, E., Giulianotti, P. C., Sbrana, F., Balestracci, T., Giulini, S. M., Pellizzari, A., Ronconi, M., Cimaschi, S., Grassini, M., Lacignola, S., Calandro, L., Mazzitelli, L., Costarella, S. M., Egidio, A., Mello Teggia, P., Stefano, E., Cassini, P., Modica, G., Lupo, F., Giraci, G., Mosca, F., Del Chiaro, M., Mosella, G., Benassai, G., Nanni, M., D'Aristotile, A., Negro, P., Pirazzoli, A., Rabitti, P. G., Romano, C., Gerardi, G., Troianello, B., Russello, D., Di Stefano, A., Avelli, S., Salval, N., Bellini, N., Scalon, P., Staudacher, C., Parolini, D., Strazzabosco, M., Signorelli, S., Tedeschi, U., Testoni, P. A., Masci, E., Mariani, A., Torelli, E., Garcea, M. R., Lombardi, V., Lecconi, L., Valeri, L., Presenti, L., Alessio, F., Ventrucci, M., Virzi, S., Cipolla, A., De Rai, P, Zerbi, A, Castoldi, L, Bassi, C, Frulloni, L, Uomo, G, Gabbrielli, A, Pezzilli, R, Cavallini, G, Di Carlo, V, Proinf, Aisp, and Testoni, PIER ALBERTO

Subjects
Male, Time Factors, Pancreatitis, Alcoholic, medicine.medical_treatment, Acute pancreatitis, Surgery, Aged, Biliary Tract Diseases, Chi-Square Distribution, Cholangiopancreatography, Endoscopic Retrograde, Cholecystectomy, Laparoscopic, Female, Guideline Adherence, Humans, Italy, Pancreatitis, Pancreatitis, Acute Necrotizing, Practice Guidelines as Topic, Prospective Studies, Risk Assessment, Risk Factors, Severity of Illness Index, Treatment Outcome, Cholecystectomy, Jejunostomy, Pancreatectomy, Practice Patterns, Physicians', Hepatology, Gastroenterology, Physician's Practice Patterns, Practice Patterns, surgery, Endoscopic Retrograde, Medicine, Cholangiopancreatography, Laparoscopic, Acute Necrotizing, Alcoholic, Prospective cohort study, health care economics and organizations, acute pancreatitis, Corrigendum, Risk assessment, medicine.medical_specialty, education, Severity of illness, Intensive care medicine, Physicians', business.industry, General surgery, Original Articles, medicine.disease, business
Abstract

OBJECTIVE: This study aimed to evaluate the surgical treatment of acute pancreatitis in Italy and to assess compliance with international guidelines. METHODS: A series of 1173 patients in 56 hospitals were prospectively enrolled and their data analysed. RESULTS: Twenty-nine patients with severe pancreatitis underwent surgical intervention. Necrosectomy was performed in 26 patients, associated with postoperative lavage in 70% of cases. A feeding jejunostomy was added in 37% of cases. Mortality was 21%. Of the patients with mild pancreatitis, 714 patients with a biliary aetiology were evaluated. Prophylactic treatment of relapses was carried out in 212 patients (36%) by cholecystectomy and in 161 using a laparoscopic approach. Preoperative endoscopic retrograde cholangiopancreatography was associated with cholecystectomy in 83 patients (39%). Forty-seven patients (22%) were treated at a second admission, with a median delay of 31 days from the onset of pancreatitis. Eighteen patients with severe pancreatitis underwent cholecystectomy 37.9 days after the first admission. There were no deaths. DISCUSSION: The results indicate poor compliance with published guidelines. In severe pancreatitis, early surgical intervention is frequently performed and enteral feeding is seldom used. Only a small number of patients with mild biliary pancreatitis undergo definitive treatment (i.e. cholecystectomy) within 4 weeks of the onset of pancreatitis.

Published
2010
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29. The incidence of cardiovascular events is largely reduced in hyper-Lp(a) patients on lipoprotein apheresis. The G.I.L.A. (gruppo interdisciplinare ldl aferesi) pilot study

Author

Bigazzi, F., primary, Sbrana, F., additional, Berretti, D., additional, Zenti, M.G., additional, Zambon, S., additional, Fabris, A., additional, Fonda, M., additional, D'Alessandri, G., additional, Dal Pino, B., additional, Pianelli, M., additional, Luciani, R., additional, Ripoli, A., additional, Rafanelli, D., additional, Colpo, A., additional, Cattin, L., additional, and Sampietro, T., additional

Published
2017
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31. Poster Session 1The imaging examination and quality assessmentP185Why did the normal values of the left and right atrial volumes increase in the recent chamber quantification guidelines update?P186Atrial electromechanical delay, Left Atrial mechanical functions and longitudinal left ventricular strain in pre-diabetic patientsP187A web-based platform for e-training in echocardiographyP188Righ atrial size as a marker of success in electrical cardioversion in patients with persistent atrial fibrillationP189Echocardiographic assessment of left atrial dimensions and function in a healthy populationP190Impact of carotid artery revascularization on the cognitive and functional outcome and cerebral flow on TCD and brain MRI in patients with symptomatic carotid artery stenosis: a preliminary reportP191Aortic elasticity is impaired in hypereosinophilic syndromeP192Disturbed intracardiac flow transit prognosticates early decompensation in dilated cardiomyopathyP193Ultrasound guided treatment in acute heart failureP194Determinants of impaired global longitudinal function in middle-aged subjects free of cardiovascular diseaseP195Left ventricular remodeling in asymptomatic heart failure: classification and prognostic evaluationP196Restricted displacement of lateral right ventricular wall: a physiopathological explanation of geometrical and functional cardiac changes after cardiac surgeryP197A novel method to image intracardiac flow stagnation for the risk stratification for thrombosisP198Magnetic resonance imaging of anomalous origin of the left coronary artery from the pulmonary artery in children older than 4 monthsP199Coronary flow reserve is improved by LDL apheresis in patients with familial hypercholesterolemia and chronic ischemic heart diseaseP200 High velocities in the proximal part of the coronary arteries during routine echocardiography can predict nearest prognosisP201Recovery potential of the right ventricular function in the setting of a first STEMI treated by primary PCI: an echocardiographic studyP202Severe aortic stenosis patients with preserved ejection fraction according to flow and gradient classification: prevalence and outcomesP203Is basal left ventricular ejection time able to predict the severity of aortic stenosis in patients with depressed ejection fraction?P204Acceleration time in aortic stenosis: a new echocardiographic diagnostic parameterP205Application of novel Doppler indices of stenosis severity in the assessment of rheumatic mitral stenosis beyond conventional valve area and transvalvular gradientsP206Comparison of conventional echo score in patients with symptomatic rheumatic mitral stenosis: transesophageal echocardiography versus transthoracic echocardiographyP207Speckle-tracking echocardiography in evaluation early left ventricular systolic dysfunction in asymptomatic aortic regurgitation patients with good left ventricular ejection fractionP208Expansible aortic ring annuloplasty: mid-term results of aortic valve repairP209Papillary muscle dysfunction: insights into mitral valve prolapse using speckle tracking imaging

Author

Kebed, K., primary, Moustafa, TAMER, primary, Conte, R., primary, Doering, C., primary, Van Grootel, R W J, primary, Badacz, R., primary, Nemes, A., primary, Uejima, T., primary, Oehman, J., primary, Ceponiene, I., primary, Fabiani, I., primary, Garcia Martin, A., primary, Nishikawa, H., primary, Jurko, AJR, primary, Pasanisi, E., primary, Zagatina, A., primary, Stoian, M., primary, Monteagudo Ruiz, JM., primary, Lazaro Mendes, AS., primary, Ruiz Fernandez, D., primary, Chong, A., primary, Park, YH., primary, Mizariene, V., primary, Hlubocka, Z., primary, Ring, L., primary, Kruse, E., additional, Addetia, K., additional, Ciszek, B., additional, Thykattil, M., additional, Guile, B., additional, Lang, RM., additional, Mor-Avi, V., additional, Mahfouz, RAGAB, additional, Elzayat, AHMED, additional, Goda, MOHAMD, additional, Gad, MARWA, additional, Sansone, F., additional, Napoli, F., additional, Tonacci, A., additional, Raciti, M., additional, Landi, P., additional, Grande, A., additional, Ait-Ali, L., additional, Sveric, K., additional, Richter, U., additional, Strasser, RH., additional, Wunderlich, C., additional, Menting, ME., additional, Mcghie, JS., additional, Strachinaru, M., additional, Vletter, WB., additional, Geleijnse, ML., additional, Roos-Hesselink, JW., additional, Van Den Bosch, AE., additional, Kablak-Ziembicka, A., additional, Urbanczyk-Zawadzka, M., additional, Banys, RP., additional, Musialek, P., additional, Pieniazek, P., additional, Mleczko, S., additional, Zmudka, K., additional, Przewlocki, T., additional, Marton, I., additional, Domsik, P., additional, Kalapos, A., additional, Posfai, E., additional, Modok, S., additional, Borbenyi, Z., additional, Forster, T., additional, Takahashi, L., additional, Nishikawa, H., additional, Semba, H., additional, Sawada, H., additional, Yamashita, T., additional, Jurkevicius, R., additional, Petkeviciene, J., additional, Gustiene, O., additional, Tamuleviciute-Prasciene, E., additional, Motiejunaite, J., additional, Slapikas, R., additional, Pugliese, NR., additional, La Carrubba, S., additional, Antonini Canterin, F., additional, Colonna, P., additional, Caso, P., additional, Benedetto, F., additional, Citro, R., additional, Carerj, S., additional, Di Bello, V., additional, Moya Mur, JL., additional, Lazaro Rivera, C., additional, Rincon Diaz, LM., additional, Miguelena Hycka, J., additional, Garcia Lledo, A., additional, Jimenez Nacher, JJ., additional, Fernandez-Golfin, C., additional, Rodriguez-Roda, J., additional, Zamorano, JL., additional, Uejima, T., additional, Jurko, A., additional, Jurko, T., additional, Mistinova-Polakova, J., additional, Sbrana, F., additional, Petersen, C., additional, Bigazzi, F., additional, Dal Pino, B., additional, Coceani, M., additional, Ripoli, A., additional, Pianelli, M., additional, Luciani, R., additional, Sampietro, T., additional, Zhuravskaya, N., additional, Vareldzhyan, Y., additional, Kamenskikh, M., additional, Shmatov, D., additional, Zamfir, D., additional, Vijiiac, A., additional, Pitic, D., additional, Tamasescu, G., additional, Onciul, S., additional, Onut, R., additional, Stefan, C., additional, Dorobantu, M., additional, Gonzalez-Gomez, A., additional, Izurieta, C., additional, Marco, A., additional, Alonso Salinas, GL., additional, Hinojar Baydes, R., additional, Garcia Martin, A., additional, Casas Rojo, E., additional, Ferreira, AR., additional, Moura Ferreira, J., additional, Leite, L., additional, Oliveira, AP., additional, Ribeiro, N., additional, Barbosa, AJ., additional, Mata Martins, R., additional, Ramos, D., additional, Pego, M., additional, Gamaza Chulian, S., additional, Diaz Retamino, E., additional, Camacho Freire, S., additional, Gutierrez Barrios, A., additional, Oneto Otero, J., additional, Bansal, M., additional, Grewal, HK., additional, Kasliwal, RR., additional, Wahi, S., additional, Lee, SH., additional, Lee, DS., additional, Hwang, JM., additional, Kim, JS., additional, Kim, JH., additional, Chun, KJ., additional, Bieseviciene, M., additional, Verseckaite, R., additional, Jonkaitiene, R., additional, Janenaite, J., additional, Dostalova, G., additional, Hlubocky, J., additional, Novotny, R., additional, Vondracek, V., additional, Lindner, J., additional, Linhart, A., additional, and Preston, NK., additional

Published
2016
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32. Near-field spectroscopy of porous silicon microcavity samples

Author

Fuso, F., Labardi, M., Sbrana, F., Pardi, L., Allegrini, M., Gaburro, Z., and Pavesi, L.

Subjects
Near field microscopy -- Usage, Silicon -- Research, Silicon -- Optical properties, Physics
Abstract

The peculiar excitation features accessible by near-field optical microscopy have prospect for coupling flight sources to microoptical devices. Near-field scanning optical microscopy (NSOM) is used to carry out near-field optical spectroscopy (NFOS).

Published
2002

34. A dangerous job

Author

Viaggi, B, Sbrana, F, and Tascini, C

Published
2014

44. Surgical management of acute pancreatitis in Italy: lessons from a prospective multicentre study

Author

DE RAI, P, Zerbi, A, Castoldi, L, Bassi, C, Frulloni, L, Uomo, G, Gabbrielli, A, Pezzilli, R, Cavallini, G, DI CARLO, V, PROINF AISP PROGETTO INFORMATIZZATO PANCREATITE ACUTA, ASSOCIAZIONE ITALIANA PER LO STUDIO DEL PANCREAS COMPUTERIZED PROJECT ON ACUTE PANCREATITIS, COLLABORATORS AGUGIARO S, ITALIAN ASSOCIATION FOR THE STUDY OF THE PANCREAS STUDY G. R. O. U. P., Turri, L, Bartoli, A, Barberini, F, Cavazzoni, G, Bartolo, F, Papa, Dd, Bassi, Nicolo', Massani, M, Benedetti, A, Macarri, G, Piergallini, L, Briani, G, Bartolasi, L, Bugnano, L, Buonanno, Gm, Esposito, C, Cordovana, A, Cavina, E, Seccia, M, Lippolis, P, Musco, B, Barletta, M, Chilovi, E, DE GUELFI, A, Chirletti, P, Caronna, R, Scozzafava, S, Cardi, M, Cirino, E, Buffone, A, Colangelo, E, Caracino, V, Cortese, F, Casentini, A, Costamagna, G, Trincali, A, Curzio, M, Clivio, S, Segato, S, D'Alessandro, A, Ambrosiani, V, D'Ambrosio, B, Chiodo, C, Dicillo, M, Reale, L, Grandolfo, A, Fabbrucci, P, Bruscino, A, Mugnaini, P, Ferrarese, S, Ugenti, I, Forte, Gb, Rocco, P, Franzãˆ, A, Bertelãˆ, A, Sereni, G, Friedman, D, Mariani, L, Morelli, F, Gai, V, Antro, C, Garcea, D, Gardini, A, Lucci, E, Giulianotti, Pc, Sbrana, F, Balestracci, T, Giulini, Sm, Pellizzari, A, Ronconi, M, Cimaschi, S, Grassini, M, Lacignola, S, Calandro, L, Mazzitelli, L, Costarella, Sm, Egidio, A, Teggia, Pm, Stefano, E, Cassini, P, Modica, G, Lupo, F, Giraci, G, Mosca, F, DEL CHIARO, M, Mosella, G, Benassai, G, Nanni, M, D'Aristotile, A, Negro, P, Pirazzoli, A, Rabitti, Pg, Romano, C, Gerardi, G, Troianello, B, Russello, D, DI STEFANO, A, Avelli, S, Salval, N, Bellini, N, Scalon, P, Staudacher, C, Parolini, D, Strazzabosco, M, Signorelli, S, Tedeschi, U, Testoni, Pa, Masci, E, Mariani, A, Torelli, E, Garcea, Mr, Lombardi, V, Lecconi, L, Valeri, L, Presenti, L, Alessio, F, Ventrucci, M, Virzi, S, and Cipolla, A.

Published
2010

46. New Proteins orthologous to cerato-platanin in various Ceratocystis species and the purification and characterization of the cerato populin from Ceratocistis populonica

Author

Comparini C., Carresi l., Pagni E., Sbrana F., Sebastiani F., Luchi N., Santini A., Capretti P., Tiribilli B., Pazzagli L., Cappugi G., and Scala A.

Subjects
MALDI-TOF, PAMP activity, Induction of plant resistance, Circular dichroism, Orthologous genes - Populus sp
Abstract

Natural variants of cerato-platanin (CP), a pathogen associated molecular pattern (PAMP) protein produced by Ceratocystis platani (the causal agent of the plane canker stain), have been found to be produced by other four species of the genus Ceratocystis, including five clones of Ceratocystis fimbriata isolated from different hosts. All these fungal strains were known to be pathogenic to plants with considerable importance in agriculture, forestry, and as ornamental plants. The putative premature proteins were deduced on the basis of the nucleotide sequence of genes orthologous to the cp gene of C. platani; the deduced premature proteins of Ceratocystis populicola and Ceratocystis variospora reduced the total identity of all the others from 87.3% to 60.3%. Cerato-populin (Pop1), the CP-orthologous protein produced by C. populicola, was purified and characterized. Pop1 was a well-structured ?/? protein with a different percentage of the ?-helix than CP, and it self-assembled in vitro in ordered aggregates. Moreover, Pop1 behaved as PAMP, since it stimulated poplar leaf tissues to activate defence responses able to reduce consistently the C. populicola growth.

Published
2009

49. New proteins orthologous to cerato-platanin in various Ceratocystis species and the purification and characterization of cerato-populin from Ceratocystis populicola

Author

Comparini C., Carresi L., Pagni E., Sbrana F., Sebastiani F., Luchi N., Santini A., Capretti P., Tiribilli B., Pazzagli L., Cappugi G., and Scala A.

Subjects
MALDI-TOF, PAMP activity, Orthologous genes, Induction of plant resistance, Circular dichroism
Abstract

Natural variants of cerato-platanin (CP), a pathogen associated molecular pattern (PAMP) protein produced by Ceratocystis platani (the causal agent of the plane canker stain), have been found to be produced by other four species of the genus Ceratocystis, including five clones of Ceratocystis fimbriata isolated from different hosts. All these fungal strains were known to be pathogenic to plants with considerable importance in agriculture, forestry, and as ornamental plants. The putative premature proteins were deduced on the basis of the nucleotide sequence of genes orthologous to the cp gene of C. platani; the deduced premature proteins of Ceratocystis populicola and Ceratocystis variospora reduced the total identity of all the others from 87.3% to 60.3%. Cerato-populin (Pop1), the CP-orthologous protein produced by C. populicola, was purified and characterized. Pop1 was a well-structured alfa/beta protein with a different percentage of the alfa-helix than CP, and it self-assembled in vitro in ordered aggregates. Moreover, Pop1 behaved as PAMP, since it stimulated poplar leaf tissues to activate defence responses able to reduce consistently the C. populicola growth.

Published
2009

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