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128 results on '"Stout, Tom A.E."'

2. Effect of duration of estradiol exposure on embryo survival and endometrial gene expression in anestrous embryo recipient mares

Author

Sant´Anna Monteiro da Silva, Elisa, primary, Sanches Oquendo Júnior, Pedro, additional, Gaspari Oquendo, Fabiana Maddalena de, additional, Stout, Tom A.E., additional, de Ruijter-Villani, Marta, additional, Rodrigues, Tamiris Sabrina, additional, Beletti, Marcelo Emílio, additional, and Cuervo-Arango, Juan, additional

Published
2024
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18. Overfeeding Extends the Period of Annual Cyclicity but Increases the Risk of Early Embryonic Death in Shetland Pony Mares

Author

D’fonseca, Nicky M.M., Gibson, Charlotte M.E., Hummel, Iris, van Doorn, David A., Roelfsema, Ellen, Stout, Tom A.E., van den Broek, Jan, de Ruijter-Villani, Marta, LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, Equine Internal Medicine, dES AVR, Voortplanting paard, dES/dFAH FR, FAH theoretische epidemiologie, CS_Fertility, CS_Welfare & emerging diseases, LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, Equine Internal Medicine, dES AVR, Voortplanting paard, dES/dFAH FR, FAH theoretische epidemiologie, CS_Fertility, and CS_Welfare & emerging diseases

Subjects
endocrine system, obesity, media_common.quotation_subject, animal diseases, biology.animal_breed, Fertility, Embryonic death, Biology, Article, Anestrus, Andrology, Hemorrhagic anovulatory follicle, Shetland pony, anestrus, lcsh:Zoology, medicine, Conceptus, embryonic death, Obesity, lcsh:QL1-991, reproductive and urinary physiology, media_common, Estrous cycle, lcsh:Veterinary medicine, General Veterinary, urogenital system, Incidence (epidemiology), Embryo, Insulin dysregulation, insulin dysregulation, medicine.disease, veterinary(all), Embryo transfer, embryonic structures, lcsh:SF600-1100, Animal Science and Zoology, sense organs, hemorrhagic anovulatory follicle
Abstract

Obesity has been associated with altered reproductive activity in mares, and may negatively affect fertility. To examine the influence of long-term high-energy (HE) feeding on fertility, Shetland pony mares were fed a diet containing 200% of net energy (NE) requirements during a three-year study. The incidence of hemorrhagic anovulatory follicles (HAF) and annual duration of cyclicity were compared to those in control mares receiving a maintenance diet. Day-7 embryos were flushed and transferred between donor and recipient mares from both groups, the resulting conceptuses were collected 21 days after transfer to assess conceptus development. HE mares became obese, and embryos recovered from HE mares were more likely to succumb to early embryonic death. The period of annual cyclicity was extended in HE compared to control mares in all years. The incidence of HAFs did not consistently differ between HE and control mares. No differences in embryo morphometric parameters were apparent. In conclusion, consuming a HE diet extended the duration of cyclicity, and appeared to increase the likelihood of embryos undergoing early embryonic death following embryo transfer.

Published
2021
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19. Overfeeding extends the period of annual cyclicity but increases the risk of early embryonic death in shetland pony mares

Author

LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, Equine Internal Medicine, dES AVR, Voortplanting paard, dES/dFAH FR, FAH theoretische epidemiologie, CS_Fertility, CS_Welfare & emerging diseases, D’fonseca, Nicky M.M., Gibson, Charlotte M.E., Hummel, Iris, van Doorn, David A., Roelfsema, Ellen, Stout, Tom A.E., van den Broek, Jan, de Ruijter-Villani, Marta, LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, Equine Internal Medicine, dES AVR, Voortplanting paard, dES/dFAH FR, FAH theoretische epidemiologie, CS_Fertility, CS_Welfare & emerging diseases, D’fonseca, Nicky M.M., Gibson, Charlotte M.E., Hummel, Iris, van Doorn, David A., Roelfsema, Ellen, Stout, Tom A.E., van den Broek, Jan, and de Ruijter-Villani, Marta

Published
2021

20. Effect of long-term overfeeding of a high-energy diet on glucose tolerance in Shetland pony mares

Author

d' Fonseca, Nicky M.M., Gibson, Charlotte M.E., van Doorn, David A., de Ruijter-Villani, Marta, Stout, Tom A.E., Roelfsema, Ellen, Voortplanting paard, LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, dES/dFAH FR, Equine Internal Medicine, dES AVR, CS_Fertility, and CS_Welfare & emerging diseases

Subjects
obesity, glucose metabolism, insulin dysregulation, horse
Abstract

Background: Overfeeding is associated with obesity and insulin dysregulation (ID), which are both risk factors for equine metabolic syndrome. How chronic overfeeding affects development of these factors is poorly understood. Objectives: To examine the influence of long-term high-energy diet provision on body condition and ID. Animals: Eleven Shetland pony mares. Methods: In a 3-phase study, the high-energy group (n = 7) was fed 200% of net energy (NE) requirements (hay; concentrate: 36% sugar and starch, 13% fat) for 24 weeks, followed by 17 weeks hay-only feeding before resuming the high-energy diet (n = 4) for an additional 29 weeks. Mares were weighed weekly. Oral glucose tolerance tests were performed 3 to 4 times per dietary period. Results were compared with those of a control group (phase 1, n = 4; phases 2 and 3, n = 6) that received 100% NE requirements, using a general linear mixed model with post hoc Bonferroni testing. Results: The mean body weight of the high-energy group increased by 27% per high-energy feeding period. During both feeding periods, area under the curve (AUC) for plasma glucose concentration decreased (P

Published
2020

21. Effect of long-term overfeeding of a high-energy diet on glucose tolerance in Shetland pony mares

Author

Voortplanting paard, LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, dES/dFAH FR, Equine Internal Medicine, dES AVR, CS_Fertility, CS_Welfare & emerging diseases, d' Fonseca, Nicky M.M., Gibson, Charlotte M.E., van Doorn, David A., de Ruijter-Villani, Marta, Stout, Tom A.E., Roelfsema, Ellen, Voortplanting paard, LS Voortplanting Inwendige Ziekten, FAH voeding, dFAH AVR, dES/dFAH FR, Equine Internal Medicine, dES AVR, CS_Fertility, CS_Welfare & emerging diseases, d' Fonseca, Nicky M.M., Gibson, Charlotte M.E., van Doorn, David A., de Ruijter-Villani, Marta, Stout, Tom A.E., and Roelfsema, Ellen

Published
2020

23. Contributors

Author

Alvarenga, Marco A., primary, Antczak, Doug. F., additional, Bergfelt, Don R., additional, Card, Claire, additional, Carnevale, Elaine M., additional, Chenier, Tracey S., additional, Gomez, Isabel, additional, Hurtgen, John P., additional, da Cruz Landim-Alvarenga, Fernanda, additional, Love, Charles C., additional, Lyle, Sarah K., additional, Matthews, Phil, additional, McDonnell, Sue M., additional, Meyers, Stuart, additional, Paccamonti, Dale, additional, Parsons, Deborah A., additional, Pinto, Nelson, additional, Pycock, Jonathan F., additional, Riera, Fernando L., additional, Roser, Janet F., additional, Sebastian Rodriguez, Jacobo, additional, Sanchez, Roberto, additional, Sieme, Harald, additional, Sprayberry, Kim A., additional, Steiner, John V., additional, Stout, Tom A.E., additional, Tibary, Ahmed, additional, and Umphenour, Norman W., additional

Published
2009
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29. Potential Health and Environmental Risks of Three-Dimensional Engineered Polymers

Author

De Almeida Monteiro Melo Ferraz, Marcia, Henning, Heiko H.W., Da Costa, Pedro Ferreira, Malda, Jos, Le Gac, Séverine, Bray, Fabrice, Van Duursen, Majorie B.M., Brouwers, Jos F., Van De Lest, Chris H.A., Bertijn, Ingeborg, Kraneburg, Lisa, Vos, Peter L.A.M., Stout, Tom A.E., Gadella, Barend M., De Almeida Monteiro Melo Ferraz, Marcia, Henning, Heiko H.W., Da Costa, Pedro Ferreira, Malda, Jos, Le Gac, Séverine, Bray, Fabrice, Van Duursen, Majorie B.M., Brouwers, Jos F., Van De Lest, Chris H.A., Bertijn, Ingeborg, Kraneburg, Lisa, Vos, Peter L.A.M., Stout, Tom A.E., and Gadella, Barend M.

Published
2018

30. Potential Health and Environmental Risks of Three-Dimensional Engineered Polymers

Author

Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, De Almeida Monteiro Melo Ferraz, Marcia, Henning, Heiko H.W., Da Costa, Pedro Ferreira, Malda, Jos, Le Gac, Séverine, Bray, Fabrice, Van Duursen, Majorie B.M., Brouwers, Jos F., Van De Lest, Chris H.A., Bertijn, Ingeborg, Kraneburg, Lisa, Vos, Peter L.A.M., Stout, Tom A.E., Gadella, Barend M., Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, De Almeida Monteiro Melo Ferraz, Marcia, Henning, Heiko H.W., Da Costa, Pedro Ferreira, Malda, Jos, Le Gac, Séverine, Bray, Fabrice, Van Duursen, Majorie B.M., Brouwers, Jos F., Van De Lest, Chris H.A., Bertijn, Ingeborg, Kraneburg, Lisa, Vos, Peter L.A.M., Stout, Tom A.E., and Gadella, Barend M.

Published
2018

31. Negative uterine asynchrony retards early equine conceptus development and upregulation of placental imprinted genes

Author

Gibson, Charlotte, de Ruijter-Villani, Marta, Stout, Tom A.E., LS Voortplanting Inwendige Ziekten, LS Klinische Reproductie, dES/dFAH FR, LS Voortplanting Inwendige Ziekten, LS Klinische Reproductie, and dES/dFAH FR

Subjects
0301 basic medicine, medicine.medical_specialty, Imprinted genes, media_common.quotation_subject, Uterus, Embryonic Development, Biology, Andrology, 03 medical and health sciences, Genomic Imprinting, medicine, Embryonic disc, Conceptus, Animals, Horses, Ovulation, DNA Modification Methylases, media_common, Obstetrics, urogenital system, Embryogenesis, Uterine environment, Obstetrics and Gynecology, Gene Expression Regulation, Developmental, Embryo, Embryo transfer, 030104 developmental biology, medicine.anatomical_structure, Reproductive Medicine, embryonic structures, Female, Genomic imprinting, Developmental Biology
Abstract

Introduction Placental imprinted genes appear to be sensitive indicators of an inappropriate pre-implantation environment. This study examined the effects of negative uterine asynchrony after embryo transfer (ET) on early horse embryo development, and yolk-sac membrane expression of DNA methyltransferases (DNMTs) and equine specific placental imprinted genes. Methods Day 8 embryos were transferred to recipient mares on day 8 (synchronous) or day 3 (asynchronous) after ovulation, and conceptuses were recovered 6 or 11 days later (day 14 or 19 of development). Results Day 14 conceptuses recovered from an asynchronous uterus had a smaller embryonic disc, in which primitive streak development was visibly retarded compared to conceptuses from a synchronous uterus. Similarly, length, somite number and organogenesis were retarded in day 19 embryos after asynchronous ET. Maternal ( GRB10 , H19 , IGF2R , PHLDA2 ) and paternal ( IGF2 , INSR , PEG3 , PEG10 , DIO3 , NDN , SNRPN ) imprinted genes and DNMTs ( DNMT1 , 3A and 3B ) were all up-regulated between day 14 and 19 of pregnancy and, for most, mRNA expression was higher in synchronous than asynchronous day 19 yolk-sac membrane. Expression of the paternally imprinted gene HAT1 increased between day 14 and 19 of pregnancy, but was not affected by the asynchrony. Discussion Conceptus development and upregulation of DNMTs and imprinted genes were delayed rather than dysregulated after transfer into a negatively asynchronous uterus. We propose that this ability to ‘reset’ conceptus development to uterine stage is an adaptation that explains why horse embryos are unusually tolerant of asynchrony after ET.

Published
2017

33. Improved bovine embryo production in an oviduct-on-a-chip system : prevention of poly-spermic fertilization and parthenogenic activation

Author

Ferraz, Marcia A.M.M., Henning, Heiko H.W., Costa, Pedro F., Malda, Jos, Melchels, Ferry P.W., Wubbolts, Richard, Stout, Tom A.E., Vos, Peter L.A.M., Gadella, Bart M., Ferraz, Marcia A.M.M., Henning, Heiko H.W., Costa, Pedro F., Malda, Jos, Melchels, Ferry P.W., Wubbolts, Richard, Stout, Tom A.E., Vos, Peter L.A.M., and Gadella, Bart M.

Published
2017

34. Decision Making for Cryptorchid Castration; a Retrospective Analysis of 280 Cases

Author

LS Heelkunde, LS Klinische Reproductie, LS Voortplanting Inwendige Ziekten, dES AVR, dES/dFAH FR, Huppes, Tsjester, Stout, Tom A.E., Ensink, Jos M., LS Heelkunde, LS Klinische Reproductie, LS Voortplanting Inwendige Ziekten, dES AVR, dES/dFAH FR, Huppes, Tsjester, Stout, Tom A.E., and Ensink, Jos M.

Published
2017

35. Hindquarter Movement of Sporthorse Stallions During Semen Collection

Author

Dep Gezondheidszorg Landbouwhuisdieren, dES/dFAH FR, LS Heelkunde, van Beuzekom, Emma L., Hoogendoorn, Arie C., Colenbrander, Ben, Stout, Tom A.E., Back, Willem, Dep Gezondheidszorg Landbouwhuisdieren, dES/dFAH FR, LS Heelkunde, van Beuzekom, Emma L., Hoogendoorn, Arie C., Colenbrander, Ben, Stout, Tom A.E., and Back, Willem

Published
2017

37. Improved bovine embryo production in an oviduct-on-a-chip system: prevention of poly-spermic fertilization and parthenogenic activation

Author

Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Ferraz, Marcia A.M.M., Henning, Heiko H.W., Costa, Pedro F., Malda, Jos, Melchels, Ferry P.W., Wubbolts, Richard, Stout, Tom A.E., Vos, Peter L.A.M., Gadella, Bart M., Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Ferraz, Marcia A.M.M., Henning, Heiko H.W., Costa, Pedro F., Malda, Jos, Melchels, Ferry P.W., Wubbolts, Richard, Stout, Tom A.E., Vos, Peter L.A.M., and Gadella, Bart M.

Published
2017

41. Procaine Induces Cytokinesis in Horse Oocytes via a pH-Dependent Mechanism1

Author

Leemans, Bart, primary, Gadella, Bart M., additional, Stout, Tom A.E., additional, Heras, Sonia, additional, Smits, Katrien, additional, Ferrer-Buitrago, Minerva, additional, Claes, Eline, additional, Heindryckx, Björn, additional, De Vos, Winnok H., additional, Nelis, Hilde, additional, Hoogewijs, Maarten, additional, and Van Soom, Ann, additional

Published
2015
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47. Evaluation of a turbidimetric immunoassay (TIA) for measuring IgG concentrations in mare colostrum.

Author

Sitters, Sofie, Stout, Tom A.E. (Thesis Advisor), McCue, Patrick M., Sitters, Sofie, Stout, Tom A.E. (Thesis Advisor), and McCue, Patrick M.

Abstract

A commercial turbidimetric immunoassay (ARS Foal-IgG Test; Animal Reproduction Systems, Chino, CA), designed for measuring IgG concentrations in foal serum, was evaluated as a means for quantifying IgG concentrations in the colostrum of 43 mares (28 post-partum/pre-suckle samples and 15 pre-partum samples). This TIA test was found to be unsuitable for determining IgG concentrations in either untreated or centrifuged (fat-free) mare colostrum. The IgG concentrations estimated by TIA were significantly higher than those quantified by the radial immunodiffusion (RID) assay, and there was no overall correlation between the two tests. By contrast, the BRIX (sugar) refractometer proved to be an accurate, fast and easy technique for estimating colostral IgG concentrations. The BRIX percentage score correlated highly with colostral IgG concentrations measured by RID (pre- and post-foaling samples: r=0.78, serial pre-foaling samples: r=0.88, serial post-foaling samples: r=0.99). Pre-partum colostral IgG concentrations were measured daily in 3 mares during the final 14 days of gestation. During this period, 2 mares maintained relatively constant IgG concentrations whereas the third mare showed a rise in colostral IgG starting around 12 days before parturition. Colostral IgG concentrations were also evaluated in 2 mares during the first 24 hours post-foaling. IgG concentrations dropped by 78% in the first 4-6 hours after birth, and then reached a plateau that persisted for the next 10 hours.

Published
2008

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