1. Dual-degradable and injectable hyaluronic acid hydrogel mimicking extracellular matrix for 3D culture of breast cancer MCF-7 cells.
- Author
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Suo, Aili, Xu, Weijun, Wang, Yaping, Sun, Tiantian, Ji, Lijie, and Qian, Junmin
- Subjects
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HYALURONIC acid , *HYDROGELS , *BREAST cancer , *CANCER cell culture , *HYDRAZONES - Abstract
Highlights • HA hydrogel was prepared via hydrazone-/photo- dual crosslinking process. • HA hydrogel had topography and mechanical property similar to breast tumor tissue. • HA hydrogel promoted MCF-7 cells to cluster and secrete VEGF, bFGF and IL-8. • 3D culture enhanced cell migration and invasion potentials and in vivo tumorigenesis. Abstract In tumor biology, it is widely recognized that 3D rather than 2D cell culture can recapitulate key features of solid tumors, including cell-extracellular matrix (ECM) interactions. In this study, to mimick the ECM of breast cancer, hyaluronic acid (HA) hydrogels were synthesized from two polyvalent HA derivatives through a hydrazone and photo dual crosslinking process. HA hydrogels could be formed within 120 s. The hydrogels had similar topography and mechanical properties to breast tumor and displayed glutathione and hyaluronidase dual-responsive degradation behavior. Biological studies demonstrated that HA hydrogel could support the proliferation and clustering of breast cancer MCF-7 cells. The expression levels of VEGF, IL-8 and bFGF in hydrogel-cultured cells were significantly greater than those in 2D culture. Moreover, cells from hydrogel culture exhibited greater migration/invasion abilities and tumorigenicity than 2D-cultured cells. Therefore, the HA hydrogels are a promising ECM-mimicking matrix for in vitro construction of breast cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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