1. Serine protease activity in excretory–secretory products of Oestrus ovis (Diptera: Oestridae) larvae
- Author
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Ph. Jacquiet, Guillaume Tabouret, Ph. Dorchies, L Bret-Bennis, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Inconnu, ProdInra, Migration, Unité associée de Physiopathologie respiratoire des ruminants, and Institut National de la Recherche Agronomique (INRA)
- Subjects
[SDV]Life Sciences [q-bio] ,Benzoylarginine Nitroanilide ,IMMUNOLOGIE ,Oestrus ovis ,030308 mycology & parasitology ,0302 clinical medicine ,Ovis ,ComputingMilieux_MISCELLANEOUS ,0303 health sciences ,biology ,Hydrolysis ,Serine Endopeptidases ,General Medicine ,Hydrogen-Ion Concentration ,Trypsin ,[SDV] Life Sciences [q-bio] ,Biochemistry ,Excretory system ,Larva ,Electrophoresis, Polyacrylamide Gel ,Collagen ,medicine.drug ,Proteases ,030231 tropical medicine ,Sheep Diseases ,Myiasis ,03 medical and health sciences ,medicine ,Animals ,Protease Inhibitors ,Serum Albumin ,Serine protease ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,Sheep ,General Veterinary ,Diptera ,fungi ,Mucin ,Mucins ,biology.organism_classification ,medicine.disease ,GS PAGE ,Molecular Weight ,Chromogenic Compounds ,Immunoglobulin G ,biology.protein ,Cattle ,Parasitology ,Azo Compounds - Abstract
The sheep bot fly, Oestrus ovis, is a very common myiasis of nasal and sinus cavities of sheep and goats causing severe welfare and production implications. As the viability of O. ovis adult flies strictly depends on larval abilities to assimilate and to stock nutrients from the host, it was necessary to investigate proteolytic activities in larval excretory/secretory products (ESP). ESP of O. ovis larvae degrade mucosal and plasmatic components such as mucin, albumin or immunoglobulin G. A preliminary biochemical characterization, using substrate gel analysis and inhibitor sensitivity, demonstrated the presence of at least six major serine proteases (molecular weights from 20 to 100 kDa), mainly trypsin-like, secreted in the digestive tube of larvae. Their involvement in larval trophic activity and evasion from the host immune response is further discussed as O. ovis excretory/secretory serine proteases could represent potential vaccinal targets.
- Published
- 2003