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Under in vitro cultivation of 14 soft tissue malignant human tumors there were obtained two cell lines: from cells of hypernephroma (Hn-7) and malignant paraganglioma (Par-1). The transfer of the cultured Par-1 cells medium to recipient cells CET enabled obtaining the transformed cell line 63. This line possesses all the features of in vitro growing tumor cells: it results in tumor growth in young Syrian hamsters, when injected retrobuccally, and in newborn animals injected percutaneously, also it yields cell colonies growth in semiliquid agar. The cultured line 63 ran through more than 100 passages, its isoenzymic assay indicates that it belongs to a human type and differs from cells HeLa, the karyotypic pattern shows the number of chromosomes to be close to the dyploid number in man. In the cell line 63 there is the synthesis of two viruses: RNA-containing one belonging to the orthomixo-group and DNA-containing one belonging to human oncoviruses of the Papova group.

The line of human embryo fibroblasts transformed by Rous sarcoma virus (strain Schmidt-Ruppin) contained and produced Rous sarcoma virus; this was shown by the complement fixation test, immunofluorescent test, electron microscopy and labelling with 3H-uridine peak in sucrose gradient. Biological properties of the new synthesized virus differed from that of the parent Schmidt-Ruppin strain; the range of the sensitive cells and the protein envelope antigens altered in particular. Analogous results by the change of the viral biological properties produced in the unnatural host tissue were obtained for polioma virus synthesized in the human embryo fibroblasts transformed by this virus.

A stable tumorigenic cell line has been obtained from the human hypernephrome. The culture grew as monolayers of large polygonal cells with foamy cytoplasm and 1-2 nucleoli. The cells formed monolayer after the third day after planting into flask and then formed multilayered growth. The electron microscopic studies of hypernephrome sections revealed the dark and light cells. The hypernephrome cells have characteristics of tumor cells. On the 200th passage the cells are aneuploid with a model number of 62 chromosomes; an acrocentric chromosome in D group is noted as a marker one. The hypernephrome cells are free of Mycoplasma contamination, able to support the several virus replication.