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Gambusia affinis (Baird and Girard, 1853), a notorious non-native invasive fish species, has negatively impacted aquatic ecosystems around the world. This species was recently identified in Lake Kenyir, one of the largest impoundments in South East Asia, using DNA barcoding. The coxI sequence of Gambusia caught in Lake Kenyir was compared with the sequences of topotypic voucher specimens of G. affinis and two other candidate Poeciliidae. The species was found to cluster with G. affinis but not with monophyletic clades of either G. holbrooki or P. reticulata thus confirming species identity. The fish is yet to be widely established in the lake with the current distribution limited to areas of anthropogenic disturbance.

This report presents the scientific basis and development of the Ecological Impact Assessment (EIA) tools for fluvial flooding (EIA F tool) and coastal inundation (EIA C tool). These tools have been developed within the Ecological Consequences of Flooding (ECF) project and may be used to support an environmental risk assessment. When developing plans to manage flood risk, economic, social and environmental impacts are considered. There are many tools that help to estimate the economic impacts. However, there is currently no standard approach for evaluating the impacts on the natural environment within a flood risk assessment. Impacts of floods on the natural environment are often complex and include benefits and disbenefits. The Broad Scale Ecosystem Assessment (BSEA) toolkit is based on GIS data sets (existing or producible) that already exist or can be easily created and that have an apparent relationship with ecological characteristics. It is largely left to experts to interpret the ecological implications of these data. The project reported here builds on this work by introducing more scientific knowledge and objectivity to the assessment of ecological impact by developing prototype GIS based tools that will support decision making. The prototype tools developed here will be used to provide an initial assessment of ecological assets at risk of fluvial flooding and coastal inundation. In this way it will help all Flood and Coastal Risk Management (FCRM) authorites fulfil their duties under the EU Floods Directive, Habitats Directive, Bird’s Directive and Water Framework Directive. In the future it is envisioned that the tool will be embedded within software and made available to general users through an application like the Modelling Decision Support Framework that is used by the Environment Agency in the preparation of Catchment Flood Management Plans. The tools will thus support Environmental Impact Assessments and Strategic Environmental Assessments for flood