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Your search keyword '"Wang, Yulai"' showing total 140 results
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140 results on '"Wang, Yulai"'

21. Pomegranate polyphenol punicalin ameliorates lipopolysaccharide‐induced memory impairment, behavioral disorders, oxidative stress, and neuroinflammation via inhibition of TLR4‐NF‐кB pathway.

Author

Chen, Peng, Guo, Zhilei, Lei, Jiexin, and Wang, Yulai

Abstract

Neuroinflammation may play an important role in the development of Alzheimer's disease (AD). Previous studies have reported that lipopolysaccharide (LPS)‐induced neuroinflammation causes memory impairments and behavioral disorders. We investigated the potential preventive effects of punicalin (PUN), a polyphenolic component of pomegranate, on LPS‐induced memory deficiency and anxiety‐ and depression‐like behaviors, along with the underlying mechanisms. LPS‐treated cultured microglial BV2 cells and BV2 cell/Neuro‐2a (N2a) cell coculture system were investigated for anti‐neuroinflammatory effects of PUN in vitro. The in vivo experiments involved mice administered a 4‐week course of oral gavage with 1500 mg/kg/d PUN before intraperitoneal LPS (250 mg/kg daily 7 times) injections. The in vitro results demonstrated that PUN inhibited the LPS‐induced inflammatory cytokine (IL‐18, IL‐1β, TNF‐ɑ, and IL‐6) production in BV2 cells and protected N2a cells from synaptic damage mediated by BV2 microglia‐induced neuroinflammation. In in vivo studies, it was observed that PUN improved memory impairment and anxiety‐ and depression‐like behaviors caused by LPS and reduced the expression of inflammatory proteins such as iNOS, COX‐2, IL‐1β, IL‐2, IL‐6, and TNF‐α. Furthermore, PUN inhibited the LPS‐induced production of MDA; increased the activities of CAT, SOD, and GSH‐Px, and inhibited LPS‐induced Aβ1−42 generation through down‐regulation of APP and BACE1 expression. Moreover, PUN also suppressed the expression of TLR4, IRAK4, TRAF6, IKK‐β, NF‐κB, p65, and HMGB1 in LPS‐treated mouse brain and cultured microglial BV‐2 cells. These results suggest that PUN inhibits LPS‐induced memory impairment via anti‐inflammatory and anti‐amylogenic mechanisms through inhibition of TLR4‐NF‐kB activation. [ABSTRACT FROM AUTHOR]

Published
2024
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42. Plant species influence microbial metabolic activity and butachlor biodegradation in a riparian soil from Chongming Island, China

Author

Haiyan Chen, Mengmeng Wang, Changming Yang, and Wang Yulai

Subjects
Rhizosphere, geography, geography.geographical_feature_category, biology, Soil Science, Plant community, Biodegradation, biology.organism_classification, chemistry.chemical_compound, Microbial population biology, chemistry, Agronomy, Calamus, Soil water, Botany, Butachlor, Riparian zone
Abstract

Field sampling and incubation experiment were conducted to investigate differences in microbial metabolic activity and butachlor biodegradation in riparian soils from four different plant communities such as P. australis, A. calamus, and Z. aquatica communities, and a mixed community of P. australis and A. calamus in Chongming Island, China. The results suggested that differences in rhizosphere microbial carbon substrate utilization patterns and enzymatic activities among vegetation types were significant. Catalase activity and fluorescein diacetate (FDA) hydrolysis rate and soil basal respiration (SBR) in the rhizosphere of the mixture of P. australis and A. calamus were 45%, 76%, and 62% higher, respectively, than in the rhizosphere of the pure P. australis community. Community level physiological profiles (CLPPs) via BIOLOG Ecoplates™ indicated that the mixture community of P. australis and A. calamus had the highest sole-carbon-source utilization and functional diversity of microbial community in rhizosphere soil, followed by P. australis, A. calamus, and Z. aquatica. Compared with the rhizosphere soils of the three pure plant communities, the mixture of P. australis and A. calamus displayed a significantly greater butachlor biodegradation percentage in the rhizosphere soil. The half-life for the rhizosphere soil of the mixture community of P. australis and A. calamus were 33, 51 and 57% shorter, as compared to the three pure plant communities, respectively. Our data indicate that vegetation types can exert a great effect on the biodegradation of herbicide in the riparian soil.

Published
2013

46. Serum bone remodeling parameters and transcriptome profiling reveal abnormal bone metabolism associated with keel bone fractures in laying hens

Author

Wei, Haidong, Bi, Yanju, Wang, Yulai, Zhao, Qian, Zhang, Runxiang, Li, Jianhong, and Bao, Jun

Abstract

Keel bone fractures affect welfare, health, and production performance in laying hens. A total of one hundred and twenty 35-wk-old Hy-line Brown laying hens with normal keel (NK) bone were housed in furnished cages and studied for ten weeks to investigate the underlying mechanism of keel bone fractures. At 45 wk of age, the keel bone state of birds was assessed by palpation and X-ray, and laying hens were recognized as NK and fractured keel (FK) birds according to the presence or absence of fractures in keel bone. The serum samples of 10 NK and 10 FK birds were collected to determine bone metabolism-related indexes and slaughtered to collect keel bones for RNA-sequencing (RNA-seq), Micro-CT, and histopathological staining analyses. The results showed that the concentrations of Ca, phosphorus, calcitonin, 25-hydroxyvitamin D3, and osteocalcin and activities of alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP) in serum samples of FK birds were lower than those of NK birds (P< 0.05), but the concentrations of parathyroid hormone, osteoprotegerin, and corticosterone in serum samples of FK birds were higher than those of NK birds (P< 0.05). TRAP staining displayed that FK bone increased the number of osteoclasts (P< 0.05). Micro-CT analysis indicated that FK bone decreased bone mineral density (P< 0.05). Transcriptome sequencing analysis of NK and FK bones identified 214 differentially expressed genes (DEGs) (|log2FoldChange| > 1, P< 0.05), among which 88 were upregulated and 126 downregulated. Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) analysis indicated that 14 DEGs related to skeletal muscle movement and bone Ca transport (COL6A1, COL6A2, COL6A3, PDGFA, MYLK2, EGF, CAV3, ADRA1D, BDKRB1, CACNA1S, TNN, TNNC1, TNNC2, and RYR3) were enriched in focal adhesion and Ca signaling pathway, regulating bone quality. This study suggests that abnormal bone metabolism related to keel bone fractures is possibly responded to fracture healing in laying hens.

Published
2023
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