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Recently, gestational diabetes mellitus (GDM) exhibits an obvious trend of increase in pregnant mothers and usually causes several abnormities or diseases for the offspring. Although several studies have been reported for potential molecular mechanisms, relevant genes or mutated sites have not been intensively investigated in China. In the present study, 218 pregnant mothers (GDM group: 103 individuals and control group: 115 individuals) in China were enrolled to conduct genome-wide association study (GWAS) and pathway analyses for the purpose of related genes associated with GDM in China. Our results identified 23 SNPs exhibiting closely association with GDM using multiple tests. Annotation of these 23 SNPs identified four genes (SYNPR, CDH18, CTIF, and PTGIS), which suggests that the four genes may associate with GDM. GO enrichment and KEGG pathway analysis showed that gene SYNPR, CDH18, and PTGIS were enriched or located into the pathways or process associated with glycometabolism (e.g. insulin resistance and glucose tolerance), which further indicates that the three genes may associate with the GDM. The identification of these potential genes associating with GDM enriched the potential molecular mechanisms of GDM in Asia and will provide abundant stocks for subsequent clinical verifications for better understanding the molecular mechanisms, diagnosis, drug development and clinical treatment of GDM. [ABSTRACT FROM AUTHOR]

A kind of plant material KF is selected in this paper. The material is extracted by water and then through the best extraction process, a difunctional vegetable extracted agent ( KF - CT) is made. Then it is applied on wool fabrics by dyeing. Further, some tests such as mothproof ability test, color feature values determination and fabric fastness test are carried out and studied. The results show that: the optimum extraction conditions for KF - CT obtained from the statistical analysis are 1: 10 (material to water ratio) , 90 min, 90° C , and repeat for 2 times; wool fabrics dyeing with KF - CT are able to resistant to damage by moths. And the color fastness also meets national standards. In a word, KF - CT applied on the wool fabrics shows good performance on dyeing and mothproof ability. It has favorable applied value for practical production. [ABSTRACT FROM AUTHOR]

The paper studied the thermal stability, pH stability and placed time stability of natural anti-moth finishing agent named KC-CT. Further, the effect of extracting temperature, time, material to liquor ratio, times on extraction technology were discussed by changing the parameters, and the optimal extracting process had been determined through orthogonal test. The anti-moth property of finished on wool fabric was tested by the standard FZ/T 20004-1991. The results showed that: KC-CT's thermal stability and placed time stability were very good; KC-CT was stable at pH value 4 ∼8 and relatively unstable when its pH value at 9 ∼ 10. The optimum extraction conditions for KC-CT was 100°C , 90 min, 1:20 and 2 times. Wool fabrics finished with KC-CT had got the anti-moth property at 1A level. Anti-moth property was qualified by Chinese standard. [ABSTRACT FROM AUTHOR]

To study thermal radiation properties of special materials at high temperature in aerospace fields, the ultrahigh temperature spectral emissivity measurement system with Fourier spectrometer has been established. The linearity of system is the guarantee of emissivity measurement precision. Through measuring spectral radiation signals of a blackbody source at different temperatures, the function relations between spectral signal values and blackbody spectral radiation luminance of every spectrum points were calculated with the method of multi-temperature and multi-spectrum linear fitting. The spectral radiation signals of blackbody were measured between 1 000 degrees C and 2 000 degrees C in the spectral region from 3 to 20 microm. The linear relations between spectral signal and theory line at wavelength of 4 microm were calculated and introduced. The spectral response is well good between 4 and 18 microm, the spectral linearity are less than 1% except CO2 strong absorption spectrum regions. The results show that when the errors of measured spectrum radiation and linear fitting theory lines are certain, the higher the temperature, the smaller the spectral errors on emissivity. The linearity analysis of spectrum response is good at eliminating errors caused by individual temperature' disturbance to the spectra.

Objective: To investigate the molecular mechanism of TFE (total flavone of epimedium) in the treatment of osteoporosis, and then provide experimental evidence for modernization and further development of TFE as an traditional Chinese medicine., Methods: Sixty healthy female SD rats with aged 4 months were randomly divided into three groups (including control group in which rats received sham surgery, OVX group in which ovariectomized rats didn't give any medicine after the removal of ovaries and TFE group in which ovariectomized rats administrated TFE), 20 rats in each group. Compared bone mineral density (BMD) between before operation and at 4th week after operation in order to verify the establishment of osteoporotic model (criteria: BMD decreased more than 20% at 4th week after operation). The rats in TEF group were administrated total flavone of epimedium(concentration 30 mg/ml, 10 ml/kg, qd) orally for 4 weeks. After this, killed rats to harvest the lower part of the femur and detected BMD again. Applying the reverse transcriptase-polymerase chain reaction technique (RT-PCR) to detect expression of OPG, OPGL mRNA in bone tissue., Results: (1) At 4th week after ovariectomy, the mean BMD of lumbar vertebra in TFE group fell to (0.084 +/- 0.020) g/cm2. Administrated with TFE for 4 weeks,the BMD increased to (0.112 +/- 0.009) g/cm2. There was significant improvement compare with the OVX group (P < 0.05). (2) Compared between OVX group and TFE group, The OPG mRNA expression of TFE group obviously enhanced. There was significant difference in statistics (P < 0.05). However,the promotion for OPGL mRNA expression were detected between OVX group and TFE group,there was no significant difference in statistics (P > 0.05)., Conclusion: This study showed that TFE could inhibit differentiation and maturation of osteoclast through enhancing OPG mRNA expression, accordingly,to treat osteoporosis.

Background & Objective: Our previous study showed that aloe polysaccharides (AP) could evidently decrease the mortality of irradiated mice mainly through increasing the amount of hemocytes and ameliorating immune function of mice. Whether AP can protect the cells in vitro from irradiation damage is unknown. This study was to explore radioprotective effect of AP on 3 non-tumor cell lines, and its effect on cell cycle., Methods: MTT assay was used to detect cytotoxicities of AP to normal human liver cell line Chang Liver (C. Liver), normal human embryo kidney cell line 293, and normal human umbilicus vein endothelial cell line ECV304. The 3 cell lines were treated with AP before or after irradiation. After 7-10 days normal culture, survival rate of cells was calculated by clone formation assay. Cell cycle was analyzed by flow cytometry (FCM) at different time points after irradiation., Results: 293 cells were treated with AP at different time points before and after x-ray irradiation. Survival rate of 293 cells treated with AP 30 min before x-ray irradiation was the highest (64.2%) among all groups. Evident dosage-effect relationship of AP appeared in concentration range of 12.5-50 microg/ml. After treatment of 50 microg/ml of AP, survival rates of 293, ECV304, and C. Liver cells increased from 41.5%, 46.5%, and 40.9% to 49.4%, 72.1%, and 89.1%, respectively. Irradiation caused a distinct G(2)/M block and decreased G(0)/G(1) phase population in 293 and C. Liver cells. In C. Liver cells, pretreatment of 50 mug/ml of AP increased G(0)/G(1) phase population from 31.8% to 43.8%, decreased G(2)/M phase population from 38.5% to 13.8% 6 h after irradiation; and decreased G(2)/M phase population from 22.9% to 8.7% 24 h after irradiation. In 293 cells, the same pretreatment increased G(0)/G(1) phase population from 30.1% to 45.9% 6 h after irradiation, and from 40.4% to 45.2% 24 h after irradiation accompanied by decrease of G(2)/M population from 59.6% to 54.1%., Conclusions: AP has radioprotective effect on non-tumor cells. This effect might relate to alleviating of cell cycle turbulence.

AIM:To investigate the protective effects of polydatin (PD) against injury to primarily cultured rat hepatocytes induced by CCl(4).METHODS:Rat hepatocytes were separated by methods of liver infusion in vivo and cultured medium (7.5X10(5) cells/mL). Two mL or 0.2mL was added into 24-well or 96-well plates respectively. Twenty-four hours after cell preculture, PD at concentrations of 10(-7) mol/L-10(-4)mol/L was added into each plate. At the same time injury to hepatocytes was induced by adding 10mmol/L CCl(4).Then, 0.1mL or 1mL culture solution was removed from the 96-well or 24-well plates at 6h, 12h, 24h and 48h after CCl14 intoxication respectively for the determination of GPT, GSH and MDA. At 48h, the survivability of rat hepatocytes was assayed by the MTT colormetric method.RESULTS:After CCl(4) challenge, the release of GPT and the formation of MDA in rat hepatocytes markedly increased and maintained at a high level in 48h, whereas PD with different concentrations could markedly inhibit this elevation with 10(-5)mol/L PD having the strongest effects and inhibiting rate was over 50%. PD could also improve the decreased content of GSH caused by CCl(4) in accordance with the doses used. CCl(4) evidently decreased the hepatocyte survivability from 91.0% ± 7.9% to 35.4% ± 3.8%. On the other hand, PD at 10(-7)mol/L-10(-4)mol/L could reverse this change and improve the cell survival rates to 56.1% ± 5.2%, 65.8% ± 5.0%, 88.7% ± 6.8% and 75.2% ± 7.3%, respectively.CONCLUSION: PD at 10(-7)mol/L-10(-4)mol/L could protect primarily cultured rat hepatocytes against CCl(4) induced injury.