197 results on '"Wei Hsien Wang"'
Search Results
2. Novel Caryophyllane-Related Sesquiterpenoids with Anti-Inflammatory Activity from Rumphella antipathes (Linnaeus, 1758)
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Yu-Chia Chang, Chih-Chao Chiang, Yuan-Shiun Chang, Jih-Jung Chen, Wei-Hsien Wang, Lee-Shing Fang, Hsu-Ming Chung, Tsong-Long Hwang, and Ping-Jyun Sung
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Rumphella antipathes ,antipacid ,caryophyllane ,clovane ,rumphellolide ,superoxide anion ,Biology (General) ,QH301-705.5 - Abstract
Two previously undescribed caryophyllane-related sesquiterpenoids, antipacids A (1) and B (2), with a novel bicyclo[5.2.0] core skeleton, and known compound clovane-2β,9α-diol (3), along with rumphellolide L (4), an esterified product of 1 and 3, were isolated from the organic extract of octocoral Rumphella antipathes. Their structures, including the absolute configurations were elucidated by spectroscopic and chemical experiments. In vivo anti-inflammatory activity analysis indicated that antipacid B (2) inhibited the generation of superoxide anions and the release of elastase by human neutrophils, with IC50 values of 11.22 and 23.53 μM, respectively, while rumphellolide L (4) suppressed the release of elastase with an IC50 value of 7.63 μM.
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- 2020
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3. Persistent organic pollutants in Antarctic notothenioid fish and invertebrates associated with trophic levels.
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Fung-Chi Ko, Wei-Ling Pan, Jing-O Cheng, Te-Hao Chen, Fu-Wen Kuo, Shu-Ji Kao, Chih-Wei Chang, Hsuan-Ching Ho, Wei-Hsien Wang, and Li-Sing Fang
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Medicine ,Science - Abstract
Notothenioid fish and invertebrate samples from Antarctica were collected in the austral summer of 2009, and analyzed for persistent organic pollutants (POPs), including polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs), and polybrominated diphenylethers (PBDEs), as well as δ13C and δ15N stable isotopes for trophic level determination. In this study, the POP levels in the Antarctic biota samples were found to be ranked in the following order: OCPs > PAHs >> PBDEs. The POP levels in notothenioid fish and krill correlate to trophic levels; however, the POP concentrations in intertidal benthic invertebrates are higher than in notothenioid fish implying that specific biogeochemical factors may affect bioaccumulation in the Antarctica ecosystem. Biomagnification of POPs may have a smaller role than bioconcentration in Antarctica environment. In addition to the source, transport, exposure, and absorption for each group of POPs in the short food chain in Antarctica, the biological variation among species, interaction habitats, diet and metabolism are also factors for future studies on contaminant bioaccumulation.
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- 2018
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4. Norcembranoidal Diterpenes from the Cultured-Type Octocoral Sinularia numerosa
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Wu-Fu Chen, Chen-Ting Yin, Ching-Hsiao Cheng, Mei-Chin Lu, Lee-Shing Fang, Wei-Hsien Wang, Zhi-Hong Wen, Jih-Jung Chen, Yang-Chang Wu, and Ping-Jyun Sung
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sinuleptolide ,cembranoidal diterpene ,Sinularia numerosa ,cytotoxicity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
A known norcembranoidal diterpene, 5-episinuleptolide (1), along with a new analogue, 4α-hydroxy-5-episinuleptolide (2), were isolated from a cultured-type soft coral Sinularia numerosa. The structures of 1 and 2 were elucidated on the basis of spectroscopic methods and by comparison of the data with those of the related metabolites. Cytotoxicity of metabolites 1 and 2 against a panel of tumor cells is also described. Compound 2 exhibited moderate cytotoxicity toward CCRF-CEM cells with an IC50 value 4.21 μg/mL. Preliminary SAR (structure activity relationship) information was obtained from these two compounds.
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- 2015
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5. Rumphellaoic Acid A, a Novel Sesquiterpenoid from the Formosan Gorgonian Coral Rumphella antipathies
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Hsu-Ming Chung, Wei-Hsien Wang, Tsong-Long Hwang, Lee-Shing Fang, Zhi-Hong Wen, Jih-Jung Chen, Yang-Chang Wu, and Ping-Jyun Sung
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Rumphella antipathies ,gorgonian ,rumphellaoic acid ,sesquiterpenoid ,elastase ,Biology (General) ,QH301-705.5 - Abstract
A novel sesquiterpenoid, rumphellaoic acid A (1), was isolated from the gorgonian coral Rumphella antipathies, and was found to possess a carbon skeleton that was obtained for the first time from a natural sources. The structure of 1 was elucidated by spectroscopic methods and this compound and was found to exert a moderate inhibitory effect on the release of elastase by human neutrophils.
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- 2014
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6. Rumphellols A and B, New Caryophyllene Sesquiterpenoids from a Formosan Gorgonian Coral, Rumphella antipathies
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Hsu-Ming Chung, Wei-Hsien Wang, Tsong-Long Hwang, Jih-Jung Chen, Lee-Shing Fang, Zhi-Hong Wen, Yu-Bao Wang, Yang-Chang Wu, and Ping-Jyun Sung
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Rumphella antipathies ,sesquiterpene ,caryophyllene ,rumphellol ,superoxide anion ,elastase ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Two new marine-derived caryophyllene-type sesquiterpenoids, rumphellols A and B (1 and 2), were obtained from the gorgonian coral, Rumphella antipathies, collected off the waters of Taiwan. Although caryophyllene-type sesquiterpenes are rarely found in marine organisms, compounds of this type could be principal components of R. antipathies. The structures of new Compounds 1 and 2 were determined by analysis of their spectroscopic data, including 1D and 2D NMR experiments. Caryophyllene 1 and 2 were evaluated in terms of their anti-inflammatory activity by examining their inhibitory effects on the generation of superoxide anions and the release of elastase by human neutrophils.
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- 2014
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7. Anti-Inflammatory Effect of Momordica Charantia in Sepsis Mice
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Che-Yi Chao, Ping-Jyun Sung, Wei-Hsien Wang, and Yueh-Hsiung Kuo
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Momordica charantia ,LPS ,sepsis ,anti-inflammation ,PPARs ,Organic chemistry ,QD241-441 - Abstract
Wild bitter gourd (Momordica charantia L. var. abbreviate Seringe), a common vegetable in Asia, is used in traditional medicine to treat various diseases, including inflammation. Extant literature indicates that wild bitter gourds have components that activate PPARα and PPARγ. This research probed the influence of adding wild bitter gourd to diets on inflammation responses in mice with sepsis induced by intraperitoneal injection of LPS. Male BALB/c mice were divided normal, sepsis, positive control, and three experimental groups. The latter ate diets with low (1%), moderate (2%), and high (10%) ratios of wild bitter gourd lyophilized powder. Before mice were sacrificed, with the exception of the normal group, intraperitoneal injection of LPS induced sepsis in each group; positive control group was injected with LPS after PDTC. This experiment revealed starkly lower weights in groups with added wild bitter gourd than those of the remaining groups. Blood lipids (TG, cholesterol, and NEFA) were also lower in comparison to the sepsis group, and blood glucose concentrations recovered and approached normal levels. Blood biochemistry values related to inflammation reactions indicated GOT, GPT, C-RP, and NO concentrations of groups with added wild bitter gourd were all lower than those of the sepsis group. Secretion levels of the spleen pro-inflammatory cytokines IL-1, IL-6, and TNF-α tallied significantly lower in comparison to the sepsis group, whereas secretion levels of IL-10 anti-inflammatory cytokine increased. Expression level of proteins NF-κB, iNOS, and COX-2 were significantly inhibited. Results indicate wild bitter gourd in diets promoted lipid metabolism, reducing fat accumulation, and improving low blood glucose in sepsis. Addition of wild bitter gourd can reduce inflammation biochemical markers or indicators and pro-inflammatory cytokines in the body, hence improving the inflammation responses in mice with sepsis.
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- 2014
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8. Rumphellaones B and C, New 4,5-Seco-Caryophyllane Sesquiterpenoids from Rumphella
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Hsu-Ming Chung, Wei-Hsien Wang, Tsong-Long Hwang, Jan-Jung Li, Lee-Shing Fang, Yang-Chang Wu, and Ping-Jyun Sung
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Rumphella antipathies ,rumphellaones B and C ,caryophyllane-type sesquiterpenoid ,anti-inflammatory activity ,Organic chemistry ,QD241-441 - Abstract
Two new 4,5-seco-caryophyllane sesquiterpenoids, rumphellaones B (1) and C (2), which were found to possess unprecedented γ-lactone moieties, were obtained from the gorgonian coral Rumphella antipathies. The structures of 1 and 2 were elucidated by spectroscopic methods and compound 2 was found to display modest inhibitory effects on the generation of superoxide anions and the release of elastase by human neutrophils at a concentration of 10 μg/mL
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- 2014
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9. Briarane Diterpenoids Isolated from Gorgonian Corals between 2011 and 2013
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Jyh-Horng Sheu, Yung-Husan Chen, Yu-Hsin Chen, Yin-Di Su, Yu-Chia Chang, Jui-Hsin Su, Ching-Feng Weng, Chia-Hung Lee, Lee-Shing Fang, Wei-Hsien Wang, Zhi-Hong Wen, Yang-Chang Wu, and Ping-Jyun Sung
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Gorgonacea ,briarane ,Briareum ,Dichotella ,Junceella ,Verrucella ,Biology (General) ,QH301-705.5 - Abstract
The structures, names, bioactivities and references of 138 briarane-type diterpenoids, including 87 new compounds, are summarized in this review. All the briarane-type compounds mentioned in this review article were obtained from gorgonian corals including the genus Briareum, Dichotella, Junceella and Verrucella. Some of these compounds showed potential bioactivities.
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- 2014
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10. Cladieunicellins M–Q, New Eunicellins from Cladiella sp.
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Tsung-Hung Chen, Wu-Fu Chen, Zhi-Hong Wen, Mei-Chin Lu, Wei-Hsien Wang, Jan-Jung Li, Yang-Chang Wu, and Ping-Jyun Sung
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eunicellin ,Cladiella ,cladieunicellin ,cytotoxicity ,Biology (General) ,QH301-705.5 - Abstract
Five new 7α-hydroxyeunicellin-based diterpenoids, designated as cladieunicellins M–Q (1–5), were isolated from a Formosan octocoral Cladiella sp. The structures of 1–5 were elucidated on the basis of spectroscopic methods and by comparison of the data with those of the related metabolites. Cytotoxicity of metabolites 1–5 against the human leukemia Molt 4 and HL 60 is also described. Among them, compounds 1, 3 and 5 exhibited moderate cytotoxicity toward Molt 4 cells with IC50 values 16.43, 14.17 and 15.55 μM, respectively. Preliminary SAR (structure activity relationship) information was obtained from these compounds and their analogues.
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- 2014
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11. 8-Alkylcoumarins from the Fruits of Cnidium monnieri Protect against Hydrogen Peroxide Induced Oxidative Stress Damage
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Chi-I Chang, Wan-Chiao Hu, Che-Piao Shen, Ban-Dar Hsu, Wei-Yong Lin, Ping-Jyun Sung, Wei-Hsien Wang, Jin-Bin Wu, and Yueh-Hsiung Kuo
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Chinese herb ,Cnidium monnieri ,8-alkylcoumarin ,oxidative stress ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Three new 8-alkylcoumarins, 7-O-methylphellodenol-B (1), 7-methoxy-8-(3-methyl- 2,3-epoxy-1-oxobutyl)chromen-2-one (2), and 3'-O-methylvaginol (3), together with seven known compounds (4–10) were isolated from the fruits of Cnidium monnieri. Their structures were determined by detailed analysis of spectroscopic data and comparison with the data of known analogues. All the isolates were evaluated the cytoprotective activity by MTS cell proliferation assay and the results showed that all the three new 8-alkylcoumarins exhibited cytoprotective effect on Neuro-2a neuroblastoma cells injured by hydrogen peroxide.
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- 2014
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12. Discovery of Novel Diterpenoids from Sinularia arborea
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Kuan-Hua Chen, Chang-Feng Dai, Tsong-Long Hwang, Chun-Yu Chen, Jan-Jung Li, Jih-Jung Chen, Yang-Chang Wu, Jyh-Horng Sheu, Wei-Hsien Wang, and Ping-Jyun Sung
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sinularbol ,sinularborane ,diterpenoid ,Sinularia arborea ,superoxide anion ,Biology (General) ,QH301-705.5 - Abstract
Two novel diterpenoids, sinularbols A (1) and B (2), which were found to possess a new carbon skeleton were isolated from the soft coral Sinularia arborea. The structures of compounds 1 and 2 were elucidated by spectroscopic methods and 2 displayed a moderately inhibitory effect on the generation of superoxide anion by human neutrophils.
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- 2014
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13. Secondary Metabolites from the Soft Coral Sinularia arborea
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Ping-Jyun Sung, Wei-Hsien Wang, Yin-Di Su, Jih-Jung Chen, Yu-Chia Chang, Jan-Jung Li, Kuan-Hua Chen, Chang-Feng Dai, and Mei-Chin Lu
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n/a ,Biology (General) ,QH301-705.5 - Abstract
Previous studies on the chemical constituents of soft corals belonging to the genus Sinularia have led to the isolation of a number of interesting secondary metabolites and some of these were found to possess extensive bioactivities [1–3]. Continuation investigation on the chemical constituents of the marine invertebrates collected off the waters of Taiwan, two new cembrane-type diterpenoids, arbolides A (1) and B (2), and a known steroid, crassarosterol A (3) [4], were isolated from the soft coral Sinularia arborea (family Alcyonacea) (Figure 1). In this paper, we describe the isolation, structure determination and cytotoxicity of compounds 1–3.
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- 2013
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14. Flexibilisquinone, a New Anti-Inflammatory Quinone from the Cultured Soft Coral Sinularia flexibilis
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Ping-Jyun Sung, Jyh-Horng Sheu, Jui-Hsin Su, Wei-Hsien Wang, Yen-You Lin, Yu-Fang Lin, Chao-Ying Kuo, and Zhi-Hong Wen
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flexibilisquinone ,cultured soft coral ,Sinularia flexibilis ,anti-inflammatory ,iNOS ,COX-2 ,Organic chemistry ,QD241-441 - Abstract
A new quinone derivative, flexibilisquinone (1), was isolated from the cultured soft coral Sinularia flexibilis, originally distributed in the waters of Taiwan. The structure of quinone 1 was established by extensive spectroscopic methods, particularly 1D and 2D NMR experiments. In the in vitro anti-inflammatory effects test, quinone 1 was found to significantly inhibit the accumulation of the pro-inflammatory iNOS and COX-2 proteins of the LPS-stimulated RAW264.7 macrophage cells.
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- 2013
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15. Flexibilins A–C, New Cembrane-Type Diterpenoids from the Formosan Soft Coral, Sinularia flexibilis
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Ping-Jyun Sung, Lee-Shing Fang, Yueh-Hsiung Kuo, Wei-Hsien Wang, Wan-Ping Hu, Nai-Cheng Lin, Yung-Husan Chen, Tsong-Long Hwang, Michael Yen-Nan Chiang, Mei-Chin Lu, Li-Chung Hu, and Jui-Hsin Su
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cembrane ,diterpenoid ,Sinularia flexibilis ,elastase ,Biology (General) ,QH301-705.5 - Abstract
Three new cembrane-type diterpenoids, flexibilins A–C (1–3), along with a known cembrane, (−)-sandensolide (4), were isolated from the soft coral, Sinularia flexibilis. The structures of cembranes 1–4 were elucidated by spectroscopic methods. The structure of 4, including its absolute stereochemistry, was further confirmed by single-crystal X-ray diffraction analysis. Cembrane 2 displayed a moderate inhibitory effect on the release of elastase by human neutrophils.
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- 2013
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16. Cembrane Derivatives from the Soft Corals, Sinularia gaweli and Sinularia flexibilis
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Yang-Chang Wu, Ping-Jyun Sung, Wei-Hsien Wang, Yu-Wei Chang, Yung-Husan Chen, Wu-Fu Chen, Ting-Jang Lu, Zhi-Hong Wen, Michael Yen-Nan Chiang, Li-Chung Hu, Jui-Hsin Su, and Wei-Hsuan Yen
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cembrane ,norcembrane ,Sinularia flexibilis ,Sinularia gaweli ,X-ray ,anti-inflammatory activity ,Biology (General) ,QH301-705.5 - Abstract
A new norcembranoidal diterpene, 1-epi-sinulanorcembranolide A (1), and a new cembranoidal diterpene, flexibilin D (2), were isolated from the soft corals, Sinularia gaweli and Sinularia flexibilis, respectively. The structures of new metabolites 1 and 2 were elucidated by spectroscopic methods, and compound 2 was found to significantly inhibit the accumulation of the pro-inflammatory iNOS and COX-2 proteins of the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. In addition, S. flexibilis yielded a known cembrane, 5-dehydrosinulariolide (3); the structure, including its absolute stereochemistry, was further confirmed by single-crystal X-ray diffraction analysis.
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- 2013
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17. Natural Product Chemistry of Gorgonian Corals of the Family Plexauridae Distributed in the Indo-Pacific Ocean
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Ping-Jyun Sung, Yang-Chang Wu, Wei-Hsien Wang, Yu-Chia Chang, Lee-Shing Fang, Yin-Di Su, Yung-Husan Chen, Yu-Hsin Chen, Jui-Hsin Su, Shih-Yao Kao, Li-Hsueh Wang, and Jyh-Horng Sheu
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Plexauridae ,gorgonian ,Astrogorgia ,Bebryce ,Echinomuricea ,Euplexaura ,Menella ,Biology (General) ,QH301-705.5 - Abstract
The structures, names, bioactivities and references of 105 natural products obtained from gorgonian corals belonging to the family Plexauridae with an Indo-Pacific distribution are described in this review. All compounds mentioned in this review were obtained from gorgonian corals belonging to the genera Astrogorgia, Bebryce, Echinomuricea, Euplexaura and Menella.
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- 2012
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18. Echinohalimane A, a Bioactive Halimane-Type Diterpenoid from a Formosan Gorgonian Echinomuricea sp. (Plexauridae)
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Ping-Jyun Sung, Wei-Hsien Wang, Tsong-Long Hwang, Mei-Chin Lu, Jui-Hsin Su, Hsu-Ming Chung, Li-Chung Hu, and Wei-Hsuan Yen
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halimane ,echinohalimane ,Echinomuricea ,cytotoxicity ,elastase ,Biology (General) ,QH301-705.5 - Abstract
A new halimane-type diterpenoid, echinohalimane A (1), was isolated from a gorgonian, identified as Echinomuricea sp. The structure of 1 was determined by spectroscopic methods and this compound was found to exhibit cytotoxicity toward various tumor cells and display an inhibitory effect on the release of elastase by human neutrophils. Echinohalimane A (1) is the first halimane analogue from the marine organisms belonging to phylum Cnidaria.
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- 2012
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19. Echinoclerodane A: A New Bioactive Clerodane-Type Diterpenoid from a Gorgonian Coral Echinomuricea sp.
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Ching-Hsiao Cheng, Hsu-Ming Chung, Tsong-Long Hwang, Mei-Chin Lu, Zhi-Hong Wen, Yueh-Hsiung Kuo, Wei-Hsien Wang, and Ping-Jyun Sung
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Echinomuricea ,clerodane diterpene ,echinoclerodane ,cytotoxicity ,superoxide anion ,elastase ,Organic chemistry ,QD241-441 - Abstract
A new clerodane-type diterpenoid, echinoclerodane A (1), was isolated from a Formosan gorgonian coral Echinomuricea sp. The structure of 1 was elucidated by spectroscopic methods. Echinoclerodane A (1) is the first clerodane-type compound obtained from the marine organisms belonging to the phylum Cnidaria. Echinoclerodane A (1) exhibited moderate cytotoxicity toward MOLT-4, HL-60, DLD-1 and LoVo tumor cells and inhibitory effects on the generation of superoxide anion and the release of elastase by human neutrophils.
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- 2012
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20. Bioactive Compounds from a Gorgonian Coral Echinomuricea sp. (Plexauridae)
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Jih-Jung Chen, Wei-Hsien Wang, Ping-Jyun Sung, Jan-Jung Li, Tsong-Long Hwang, Mei-Chin Lu, Lee-Shing Fang, Yang-Chang Wu, Hsu-Ming Chung, Pei-Han Hong, and Jui-Hsin Su
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Echinomuricea ,echinolabdane ,yonarasterol ,superoxide anion ,elastase ,Biology (General) ,QH301-705.5 - Abstract
A new labdane-type diterpenoid, echinolabdane A (1), and a new sterol, 6-epi-yonarasterol B (2), were isolated from a gorgonian coral identified as Echinomuricea sp. The structures of metabolites 1 and 2 were elucidated by spectroscopic methods. Echinolabdane A (1) possesses a novel tetracyclic skeleton with an oxepane ring jointed to an α,β-unsaturated-γ-lactone ring by a hemiketal moiety, and this compound is the first labdane-type diterpenoid to be obtained from marine organisms belonging to the phylum Cnidaria. 6-epi-Yonarasterol B (2) is the first steroid derivative to be isolated from gorgonian coral belonging to the genus Echinomuricea, and this compound displayed significant inhibitory effects on the generation of superoxide anions and the release of elastase by human neutrophils.
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- 2012
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21. Distribution patterns and transportation behavior of alkylphenol polyethoxylate degradation metabolites among river, port area, and coastal water bodies of Kaohsiung City, Taiwan
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Shu-Hui Lee, Ping-Chang Ku, Hsu-Ming Chung, Wei-Hsien Wang, and Te-An Kung
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Environmental Engineering ,Rivers ,Sewage ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Taiwan ,Environmental Chemistry ,Water ,General Medicine ,General Chemistry ,Pollution ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
In this study, we conducted a comprehensive study of the distribution, transportation behavior and potential ecological risk of alkylphenol polyethoxylates (APnEOs) in the aquatic environments of Kaohsiung City, Taiwan because little information is available regarding the fate of APnEOs in the water bodies of a total environment. At Love River, APnEOs concentrations were much higher at upstream of interception stations L15 (27.33 ± 1.22 μg/L) and L16 (6.31 ± 0.14 μg/L) than at downstream of interception stations L1-L14 (0.69-2.54 μg/L). Additionally, the average ethoxy (EO) chain lengths of APnEOs at L15 and L16 were longer than at L1-L14. These observations were attributed to the sluice between L14 and L15 that intercepts and accumulates untreated sewage from upstream areas and to the infrastructure of the sewage system that prevents domestic sewage from flowing downstream in the river and to the Kaohsiung Port Area. At Kaohsiung Port Area, APnEO concentrations ranging from 0.63 to 6.50 μg/L were measured. The concentration range and average EO chain length of these APnEOs were similar to those of the downstream stretch of the river, which was attributed to the mixing efficiency of the Kaohsiung Port Area and Love River through tidal exchange. At Cijin Coastal Area, APnEO concentrations ranged from 0.14 to 18.77 μg/L. Notably, the APnEO concentration of surface waters was much higher than that of bottom waters. This observation was attributed to the sewage discharged from the ocean outfall buoying up to the surface instead of mixing with surrounding bottom waters. In potential ecological risk, 19 of 39 sampling points exceeded toxic equivalency of 1 μg/L, and approximately 48.7% of the sampling points would exceed the threshold. The result provides insight into the environmental implications of APnEOs contamination in aquatic environments and useful information for environmental policy and ecological risk assessments.
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- 2021
22. Lipid profiling differentiates the effect of ambient microenriched copper on a coral as an advanced tool for biomonitoring
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Chuan-Ho Tang, Shu-Han Shi, Ching-Yu Lin, and Wei-Hsien Wang
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Animals ,Aquatic Science ,Oceanography ,Anthozoa ,Pollution ,Lipids ,Copper ,Biological Monitoring ,Environmental Monitoring - Abstract
Copper can be beneficial or harmful to coral at environmentally relevant levels, making environmental monitoring a challenging. Membrane lipids make the cell a dynamic environment according to the circumstances; thus, the lipid profile should be indicative of an environmental/physiological state. To gain more insight into the copper effect on coral health and be a basis of biomonitoring, glycerophosphocholine profiling of coral exposed to microenriched copper levels was conducted in this study. The copper microenrichments resulted in a diacritical effect of decreasing carbonic anhydrase activity, following a supplementation effect, on coral lipid metabolism. Microdifferences in copper levels are critical to determine the coral metabolic state and were therefore included in this study. In addition, an excellent quantitative model correlating the coral lipid variation with the exposed copper levels or the induced physiological effect was obtained to demonstrate its performance for biomonitoring.
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- 2021
23. Natural Product Chemistry of Gorgonian Corals of Genus Junceella—Part II
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Mei-Chin Lu, Jimmy Kuo, Jyh-Horng Sheu, Ping-Jyun Sung, Jan-Jung Li, Lee-Shing Fang, Wei-Hsien Wang, Tai-Ting Chou, Yin-Pin Cheng, Ching-Feng Weng, Chia-Hung Lee, Yang-Chang Wu, and Jui-Hsin Su
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Junceella ,gorgonian ,briarane ,Indo-Pacific Ocean ,South China Sea ,Biology (General) ,QH301-705.5 - Abstract
The structures, names, bioactivities, and references of 81 new secondary metabolites obtained from gorgonian corals belonging to the genus Junceella are described in this review. All compounds mentioned in this review were obtained from sea whip gorgonian corals Junceella fragilis and Junceella juncea, collected from the tropical and subtropical Indo-Pacific Ocean.
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- 2011
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24. Lobocrassins A–E: New Cembrane-Type Diterpenoids from the Soft Coral Lobophytum crassum
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Jui-Hsin Su, Mei-Chin Lu, Lee-Shing Fang, Ching-Feng Weng, Yueh-Hsiung Kuo, Chia-Ying Kao, Tsong-Long Hwang, Wei-Hsien Wang, Jih-Jung Chen, Jyh-Horng Sheu, Ping-Jyun Sung, and Zhi-Hong Wen
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lobocrassin ,cembrane ,Lobophytum crassum ,cytotoxicity ,superoxide anion ,elastase ,Biology (General) ,QH301-705.5 - Abstract
Five new cembrane-type diterpenoids, lobocrassins A–E (1–5), were isolated from the soft coral Lobophytum crassum. The structures of cembranes 1–5 were established by spectroscopic and chemical methods and by comparison of the spectral data with those of known cembrane analogues. Lobocrassin A (1) is the first cembranoid possessing an α-chloromethyl-α-hydroxy-γ-lactone functionality and is the first chlorinated cembranoid from soft corals belonging to the genus Lobophytum. Lobocrassins B (2) and C (3) were found to be the stereoisomers of the known cembranes, 14-deoxycrassin (6) and pseudoplexaurol (7), respectively. Lobocrassin B (2) exhibited modest cytotoxicity toward K562, CCRF-CEM, Molt4, and HepG2 tumor cells and displayed significant inhibitory effects on the generation of superoxide anion and the release of elastase by human neutrophils.
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- 2011
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25. Cladielloides A and B: New Eunicellin-Type Diterpenoids from an Indonesian Octocoral Cladiella sp.
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Yung-Husan Chen, Chia-Ying Tai, Tsong-Long Hwang, Ching-Feng Weng, Jan-Jung Li, Lee-Shing Fang, Wei-Hsien Wang, Yang-Chang Wu, and Ping-Jyun Sung
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cladielloide ,eunicellin ,octocoral ,cytotoxicity ,superoxide anion ,elastase ,Biology (General) ,QH301-705.5 - Abstract
Two new eunicellin-type diterpenoids, cladielloides A (1) and B (2), which were found to possess a 2-hydroxybutyroxy group in their structures, were isolated from an Indonesian octocoral identified as Cladiella sp. The structures of eunicellins 1 and 2 were elucidated by spectroscopic methods. Cladielloide B (2) exhibited moderate cytotoxicity toward CCRF-CEM tumor cells and this compound displayed significant inhibitory effects on superoxide anion generation and elastase release by human neutrophils.
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- 2010
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26. Carijoside A, a Bioactive Sterol Glycoside from an Octocoral Carijoa sp. (Clavulariidae)
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Chih-Yang Liu, Tsong-Long Hwang, Mei-Ru Lin, Yung-Husan Chen, Yu-Chia Chang, Lee-Shing Fang, Wei-Hsien Wang, Yang-Chang Wu, and Ping-Jyun Sung
- Subjects
Carijoa ,carijoside ,glycoside ,superoxide anion ,elastase ,cytotoxicity ,Biology (General) ,QH301-705.5 - Abstract
A new bioactive sterol glycoside, 3β-O-(3',4'-di-O-acetyl-β-D-arabinopyranosyl)-25ξ-cholestane-3β,5α,6β,26-tetrol-26-acetate) (carijoside A, 1), was isolated from an octocoral identified as Carijoa sp. The structure of glycoside 1 was established by spectroscopic methods and by comparison with spectral data for the other known glycosides. Carijoside A (1) displayed significant inhibitory effects on superoxide anion generation and elastase release by human neutrophils and this compound exhibited moderate cytotoxicity toward DLD-1, P388D1, HL-60, and CCRF-CEM tumor cells.
- Published
- 2010
- Full Text
- View/download PDF
27. Excavatoids E and F: Discovery of Two New Briaranes from the Cultured Octocoral Briareum excavatum
- Author
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Jyh-Horng Sheu, Yang-Chang Wu, Wei-Hsien Wang, Mei-Ru Lin, Ping-Jyun Sung, Bo-Yuan Chen, and Tsong-Long Hwang
- Subjects
excavatoid ,briarane ,octocoral ,Briareum excavatum ,human neutrophil ,Biology (General) ,QH301-705.5 - Abstract
Two new briarane-related diterpenoids, designated as excavatoids E (1) andF (2), were isolated from the cultured octocoral Briareum excavatum. The structures ofcompounds 1 and 2 were established on the basis of extensive spectral data analysis. Briaranes 1 and 2 were found to exhibit moderate inhibitory effects on elastase release by human neutrophils.
- Published
- 2009
- Full Text
- View/download PDF
28. Lipid profiling of coral symbiosomes in response to copper-induced carbon limitation: A metabolic effect of algal symbionts on the host immune status
- Author
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Chuan-Ho Tang, Shu-Han Shi, Hsing-Hui Li, Ching-Yu Lin, and Wei-Hsien Wang
- Subjects
Environmental Engineering ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Anthozoa ,Pollution ,Carbon ,Membrane Lipids ,Dinoflagellida ,Environmental Chemistry ,Animals ,Symbiosis ,Copper - Abstract
Copper micropollutants are known to constrain coral's assimilation of carbonate, affecting the carbon available to algal symbionts and thus inducing a light stress. However, little is known regarding the physiological relevance of lipid metabolism in coral symbiotic algae in a carbon-limited state. Membrane lipids exhibit multiple physicochemical properties that are collectively responsible for the dynamic structure of cells depending on the physiological demands of the circumstances. To gain insight into lipid metabolism's importance in this regard, glycerophosphocholine (GPC) profiling of symbiosomes in coral (Seriatopora caliendrum) exposed to environmentally relevant copper levels (2.2-7.5 μg/L) for 4 days was performed in this study. Notably, reducing the number of 22:6-processing GPCs and increasing that of lyso-GPCs likely addressed the demands of metabolizing excess light energy, such as affecting the membrane dynamics to promote mitochondrial uncoupling. The decrease in 22:6-processing GPCs additionally protected cellular membranes from elevated oxidative stress, reducing their susceptibility to peroxidation and offsetting oxidized lipid-induced effects on membrane dynamics. The change in plasmanylcholines specifically localized within the symbiosome membrane also met the membrane requirements for responding to oxidative stress conditions. Moreover, increasing the 20:4-possessing plasmanylcholines and lysoplasmanylcholines and reducing the 22:6-possessing plasmanylcholines likely resulted in an imbalance of the immune reaction, influencing the coral-algae symbiosis given the role of such plasmanylcholines in cell signaling. In summary, carbon limitations induced by copper enrichment lead to a shift in the membrane lipid profile of coral symbiosomes, accommodating themselves to light stress conditions while compromising the symbiosis's stability.
- Published
- 2021
29. Using lipidomic methodology to characterize coral response to herbicide contamination and develop an early biomonitoring model
- Author
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Shu-Han Shi, Chuan-Ho Tang, Wei-Hsien Wang, Ching-Yu Lin, and Hsing-Hui Li
- Subjects
Environmental Engineering ,Photoinhibition ,010504 meteorology & atmospheric sciences ,Coral bleaching ,Coral ,Seriatopora caliendrum ,010501 environmental sciences ,Biology ,Models, Biological ,Risk Assessment ,01 natural sciences ,Membrane Lipids ,Biomonitoring ,Environmental monitoring ,Animals ,Environmental Chemistry ,Waste Management and Disposal ,Organism ,0105 earth and related environmental sciences ,Herbicides ,Triazines ,fungi ,Photosystem II Protein Complex ,Contamination ,Anthozoa ,Pollution ,Environmental chemistry ,sense organs ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
The use of omics technologies to profile an organism's systemic response to environmental changes can improve the effectiveness of biomonitoring. In cell physiology, the dynamic characteristics of membranes can be used to identify lipid profiles that detect environmental threats and assess the health problems associated with them. The efficacy of this approach was demonstrated by profiling glycerophosphocholines (GPCs, a major membrane lipid class) in the coral Seriatopora caliendrum after exposure to Irgarol 1051. A quantitative biomonitoring model for this photosystem II herbicide was developed by correlating variations in coral lipid profile with herbicide exposure levels and degree of photoinhibition. After 4 days of exposure, the predominant changes correlated with photoinhibition were an increase in lyso-GPCs and saturated GPCs and a decrease in phosphatidylcholines with unsaturated C18 chains or a polyunsaturated C22 chain. A time-course experiment showed that most of these lipid changes occurred opposite to the initial response and that the persistent changes can be attributed to photosynthetic shortages and the membrane accommodation of photoinhibition-induced oxidative conditions. These changes can help predict risk factors leading to coral bleaching. In this study, the application of a lipidomic methodology to characterize the adaptation of coral to ambient contamination serves as a basis for advancing environmental monitoring and assessment.
- Published
- 2019
30. Determination of sulfamonomethoxine in tilapia (Oreochromis niloticus × Oreochromis mossambicus) by liquid chromatography-tandem mass spectrometry and its application pharmacokinetics study
- Author
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Shu-Hui Lee, Te-An Kung, and Wei-Hsien Wang
- Subjects
Oreochromis mossambicus ,food.ingredient ,030309 nutrition & dietetics ,Metabolite ,lcsh:TX341-641 ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,food ,Pharmacokinetics ,Tandem Mass Spectrometry ,Liquid chromatography–mass spectrometry ,Animals ,Bile ,Enterohepatic circulation ,Chromatography, High Pressure Liquid ,Pharmacology ,0303 health sciences ,Chromatography ,Sulfamonomethoxine ,biology ,lcsh:RM1-950 ,010401 analytical chemistry ,Tilapia ,Cichlids ,biology.organism_classification ,Anti-Bacterial Agents ,0104 chemical sciences ,Oreochromis ,lcsh:Therapeutics. Pharmacology ,Liver ,chemistry ,human activities ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
A precise and reliable analytical method to measure trace levels of sulfamonomethoxine (SMM) and N4-acetyl metabolite in tilapia samples using liquid chromatography-tandem mass spectrometry was developed. Optimized chromatographic separation was performed on C18 reversed-phase columns using gradient elution with methanol and 5 mmol/L of an ammonium acetate aqueous solution (adjusted to pH 3.5 using formic acid). This study investigated the pharmacokinetic properties and tissue distribution of SMM and its major metabolite N4-acetyl sulfamonomethoxine (AC-SMM) in tilapia after a single dose of 100 mg kg−1 body weight of orally administered SMM. Blood and tissues were collected between 0.5 and 192 h with 14 total sampling time points. SMM was rapidly absorbed, and extensively distributed in the bile and liver through systemic circulation. Enterohepatic circulation of SMM was observed in the tilapia body. Acetylation percentages were 45% (blood), 90% (liver), 62% (kidney), 98% (bile), and 52% (muscle). High concentrations of AC-SMM accumulated in the tilapia bile. At 192 h, AC-SMM concentration in the bile remained at 4710 μg kg−1. The ke value of AC-SMM (0.015 h−1) in the blood was lower than that of SMM (0.032 h−1). This study demonstrated effective residue monitoring and determined the pharmacokinetic properties of SMM and AC-SMM in tilapia. Keywords: Acetylation, Enterohepatic circulation, Pharmacokinetics, Sulfonamide, Tilapia
- Published
- 2019
31. Cellular membrane accommodation to thermal oscillations in the coral Seriatopora caliendrum.
- Author
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Chuan-Ho Tang, Lee-Shing Fang, Tung-Yung Fan, Li-Hsueh Wang, Ching-Yu Lin, Shu-Hui Lee, and Wei-Hsien Wang
- Subjects
Medicine ,Science - Abstract
In the present study, the membrane lipid composition of corals from a region with tidally induced upwelling was investigated. The coral community is subject to strong temperature oscillations yet flourishes as a result of adaptation. Glycerophosphocholine profiling of the dominant pocilloporid coral, Seriatopora caliendrum, was performed using a validated method. The coral inhabiting the upwelling region shows a definite shift in the ratio of lipid molecular species, covering several subclasses. Mainly, the coral possesses a higher percentage of saturated, monounsaturated and polyunsaturated plasmanylcholines and a lower percentage of polyunsaturated phosphatidylcholines. Higher levels of lyso-plasmanylcholines containing saturated or monounsaturated fatty acid chains were also revealed in coral tissue at the distal portion of the branch. Based on the physicochemical properties of these lipids, we proposed mechanisms for handling cellular membrane perturbations, such as tension, induced by thermal oscillation to determine how coral cells are able to spontaneously maintain their physiological functions, in both molecular and physical terms. Interestingly, the biochemical and biophysical properties of these lipids also have beneficial effects on the resistance, maintenance, and growth of the corals. The results of this study suggest that lipid metabolic adjustment is a major factor in the adaption of S. caliendrum in upwelling regions.
- Published
- 2014
- Full Text
- View/download PDF
32. Novel Caryophyllane-Related Sesquiterpenoids with Anti-Inflammatory Activity from Rumphella antipathes (Linnaeus, 1758)
- Author
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Tsong-Long Hwang, Ping-Jyun Sung, Lee Shing Fang, Yu-Chia Chang, Hsu Ming Chung, Chih Chao Chiang, Wei Hsien Wang, Yuan-Shiun Chang, and Jih Jung Chen
- Subjects
medicine.drug_class ,Stereochemistry ,Neutrophils ,Anti-Inflammatory Agents ,Pharmaceutical Science ,010402 general chemistry ,01 natural sciences ,Anti-inflammatory ,Article ,Rumphella antipathes ,chemistry.chemical_compound ,Structure-Activity Relationship ,In vivo ,Superoxides ,Drug Discovery ,Ic50 values ,medicine ,antipacid ,elastase ,Animals ,Humans ,lcsh:QH301-705.5 ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,IC50 ,Polycyclic Sesquiterpenes ,Bicyclic molecule ,biology ,Molecular Structure ,010405 organic chemistry ,Superoxide ,Elastase ,caryophyllane ,clovane ,Antipathes ,rumphellolide ,biology.organism_classification ,Anthozoa ,0104 chemical sciences ,lcsh:Biology (General) ,chemistry ,superoxide anion ,Leukocyte Elastase - Abstract
Two previously undescribed caryophyllane-related sesquiterpenoids, antipacids A (1) and B (2), with a novel bicyclo[5.2.0] core skeleton, and known compound clovane-2&beta, 9&alpha, diol (3), along with rumphellolide L (4), an esterified product of 1 and 3, were isolated from the organic extract of octocoral Rumphella antipathes. Their structures, including the absolute configurations were elucidated by spectroscopic and chemical experiments. In vivo anti-inflammatory activity analysis indicated that antipacid B (2) inhibited the generation of superoxide anions and the release of elastase by human neutrophils, with IC50 values of 11.22 and 23.53 &mu, M, respectively, while rumphellolide L (4) suppressed the release of elastase with an IC50 value of 7.63 &mu, M.
- Published
- 2020
33. Simultaneous determination of eleven quinolones antibacterial residues in marine products and animal tissues by liquid chromatography with fluorescence detection
- Author
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Chui-Shiang Chang, Chin-En Tsai, and Wei-Hsien Wang
- Subjects
Pharmacology ,Detection limit ,0303 health sciences ,Chromatography ,030309 nutrition & dietetics ,Chemistry ,Formic acid ,Danofloxacin ,010401 analytical chemistry ,01 natural sciences ,High-performance liquid chromatography ,0104 chemical sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Sarafloxacin ,Marbofloxacin ,Flumequine ,medicine ,Difloxacin ,Food Science ,medicine.drug - Abstract
A simple and efficient multiresidue method was developed for determining 11 quinolones (QNs; marbofloxacin, norfloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, nalidixic acid and flumequine) in chicken, pork, fish and shrimp. The analytes were extracted with 0.3% metaphosphoric acid and acetonitrile (1:1, v/v), followed by a HLB cartridge clean-up procedure. The HPLC separation was carried out on a symmetry column C-18 (250 mm×4.5 mm i.d., 5 μm) with linear gradient elution of 0.1% formic acid and acetonitrile as mobile phase and programmable fluorescence detection. The method was validated by spiking blank animals tissues at three different levels (25, 50 and 250 ng/g; except 6.25, 12.5 and 62.5 ng/g for DAN) while the linearity, detection limit, quantification limit, precision and accuracy were checked. Mean recoveries of 11 QNs from edible animal tissues were 71.7-105.3%. The limits of quantification in different muscle tissues ranged from 5.0 to 28.0 ng/g. The results showed this method was simple, rapid, sensitive and suitable for routine tests.
- Published
- 2020
34. Simultaneous determination of 18 quinolone residues in marine and livestock products by liquid chromatography/tandem mass spectrometry
- Author
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Wei-Hsien Wang, Chui-Shiang Chang, and Chin-En Tsai
- Subjects
Pharmacology ,0303 health sciences ,Chromatography ,030309 nutrition & dietetics ,Formic acid ,Danofloxacin ,010401 analytical chemistry ,Cinoxacin ,Pipemidic acid ,Piromidic acid ,01 natural sciences ,0104 chemical sciences ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry ,Ammonium formate ,medicine ,Orbifloxacin ,Difloxacin ,Food Science ,medicine.drug - Abstract
A simple and efficient LC/ESI/MS/MS method was developed to determine 18 (fluoro)quinolone (QNs) residues in milk, chicken, pork, fish and shrimp. This method is capable of screening and confirming the presence of 12 amphoteric QNs (marbofloxacin, norfloxacin, enrofloxacin, ciprofloxacin, desethylene ciprofloxacin, lomefloxacin, danofloxacin, sarfloxacin, difloxacin, ofloxacin, orbifloxacin and enoxacin) and 6 acidic QNs (oxolinic acid, nalidixic acid, flumequine, cinoxacin, piromidic acid and pipemidic acid). The drugs were extracted from matrix with acetonitrile containing 1% formic acid, diluted in 10% acetonitrile and defatted by extraction with hexane. The LC separation was conducted on an XDB C-8 (150 mm×4.6 mm, 5 μm) column with gradient elution of 20 mM ammonium formate in 0.1% formic acid-acetonitrile as the mobile phase. Mass spectrum acquisition was completed in the positive ion mode by applying multiple reaction mode (MRM). The decision limit (CCα) and detection capability (CCβ), stated in the Decision No. 2002/657/EC and the ISO standard No.11843, have been calculated in the case of nonauthorized substance. The values of CCα ranged from 0.18 to 0.68 ng/g and CCβ ranged from 0.24 to 0.96 ng/g under specified conditions.
- Published
- 2020
35. Quantitative determination of four nitrofurans and corresponding metabolites in the fish muscle by liquid chromatography-electrospray ionization-tandem mass spectrometry
- Author
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Chung-Wei Tsai, Wei-Hsien Wang, and Chuan-Ho Tang
- Subjects
Pharmacology ,0303 health sciences ,Chromatography ,Furazolidone ,030309 nutrition & dietetics ,medicine.drug_class ,Metabolite ,010401 analytical chemistry ,Ethyl acetate ,Mass spectrometry ,01 natural sciences ,0104 chemical sciences ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry ,medicine ,Furaltadone ,media_common.cataloged_instance ,Nitrofural ,European union ,Nitrofuran ,Food Science ,medicine.drug ,media_common - Abstract
A method validated based on the European Union (EU) regulations for determining the presence of furazolidone, furaltadone, nitrofurazone, nitrofurantoin and their corresponding metabolites AOZ, AMOZ, SC and AH in fish muscle was developed. Samples were acid-hydrolyzed, treated with 2-nitrobenzaldehyde and extracted with ethyl acetate. The extracts were dried in a N2 stream and redissolved in methanol: water (50: 0, v/v). Analysis was performed by LC/ESI/MS/MS. This developed method carried limits of quantification lower than 10μg/kg for the nitrofurans and 1.0μg/kg for the metabolites. It was observed that the decision limit (CCα) ranged from 2.93 to 5.01μg/kg for the nitrofurans and 0.19 to 0.43μg/kg for the metabolites. The detection capability (CCβ) was between 3.62 and 6.20μg/kg for the nitrofurans and between 0.23 and 0.54μg/kg for the metabolites. The linear calibration curve parameters in the fortified fish muscle were between 1.0-100.0μg/kg and 0.1-10.0μg/kg for the nitrofurans and metabolites, respectively.
- Published
- 2020
36. Modeling the effects of Irgarol 1051 on coral using lipidomic methodology for environmental monitoring and assessment
- Author
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Shu-Hui Lee, Chuan-Ho Tang, Ching-Yu Lin, Wei-Hsien Wang, and Pei-Pei Sun
- Subjects
0301 basic medicine ,Environmental Engineering ,Photoinhibition ,Photosystem II ,Herbicides ,Triazines ,Chemistry ,Coral ,Membrane lipids ,Seriatopora caliendrum ,Anthozoa ,Risk Assessment ,Pollution ,03 medical and health sciences ,030104 developmental biology ,Biomonitoring ,Biophysics ,Animals ,Environmental Chemistry ,Waste Management and Disposal ,Bioindicator ,Water Pollutants, Chemical ,Organism ,Environmental Monitoring - Abstract
Coral is commonly selected as a bioindicator of detecting a variety of adverse factors such as photosystem II herbicide Irgarol 1051, through measuring pan-type biomarkers. To improve the effectiveness of biomonitoring, omic technologies have recently been applied to model the systemic changes in an organism. Membrane lipids create a dynamic cell structure based on the physiological state, which offers a distinct lipid profile to specifically detect environmental threats and assess the associated health risk. To demonstrate the potential of a lipidomic methodology for biomonitoring, the glycerophosphocholine (GPC) profiles of the coral Seriatopora caliendrum were observed during 3 days of Irgarol (0.1–2.0 μg/L) exposure. The lipid profile variations were modeled based on the Irgarol dose and the coral photoinhibition levels to develop an excellent quantitative model. The predominant changes correlated with the photoinhibition, decreasing the lyso-GPCs and GPCs with lower unsaturated chains and increasing GPCs with highly polyunsaturated chains, can be related to the consequence of blocking the photosynthetic electron flow based on the associated physiological roles. Other dose-specific lipid changes led to the partial exchange of PC(O-16:0/20:5) for PC(16,0/20:5) as a first-line response to counteract the membrane opening caused by Irgarol. Increased levels of the GPCs with 20:4 or 22:6 chains, which can promote mitochondrial functionality, confirmed an elevated respiration level in the coral exposed to Irgarol levels of >0.5 μg/L. Notably, plasmanylcholines with 20:4 or 22:6 chains and phosphatidylcholines with 22:6 or 22:5 chains, which can alter their membrane material properties to mitigate organelle pre-swelling and swelling in different ways, formed in the coral exposed to the 0.5 and 2.0 μg/L Irgarol levels. Such coral adaptations further predict the health risks associated with altered physiological conditions. In this study, the lipidomic methodology is demonstrated as a potential tool for environmental monitoring and assessment.
- Published
- 2018
37. Lipid profiling of symbiosomes in scleractinian coral in response to herbicide-induced photoinhibition
- Author
-
Chuan Ho Tang, Wei Hsien Wang, Ching-Yu Lin, and Shu Han Shi
- Subjects
0106 biological sciences ,0301 basic medicine ,Photoinhibition ,medicine.diagnostic_test ,Photosystem II ,Chemistry ,Coral ,Membrane lipids ,Seriatopora caliendrum ,Plant Science ,Oxidative phosphorylation ,01 natural sciences ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,medicine ,Biophysics ,Lipid profile ,Agronomy and Crop Science ,Ecology, Evolution, Behavior and Systematics ,010606 plant biology & botany - Abstract
Photosystem II herbicides, such as Irgarol 1051, are frequently found as common contaminants in coral reefs and represent a particular threat to coral health due to the effect of photoinhibition on symbiotic algae. Membrane lipids form dynamic structures depending on cell requirements, offering a distinct lipid profile for characterizing ambient condition-induced cellular alterations. To obtain fundamental insight into the effect of herbicides on coral health, the glycerophosphocholine profiles of symbiosomes in a coral, Seriatopora caliendrum, were observed during 4 days of Irgarol exposure (50–200 ng/L). On day 1 of exposure, the predominant changes were an increase in phosphatidylcholines with 2 unsaturated chains, especially PC(22:6/22:6), a saturated plasmanylcholine PC(O-18:0/16:0) and a decrease in polyunsaturated plasmanylcholines. These changes indicated an upregulation of membrane-related functions to cope with physiological disturbances, such as increased H2O2 levels, induced by photoinhibition. However, these changes were mostly inverted or insignificant after day 1of exposure. A decrease in lyso-glycerophosphocholines and an increase in phosphatidylcholines with a polyunsaturated chain were also induced as the exposure time increased. Based on the physicochemical properties, these lipid changes can be considered to counteract lipid oxidation-induced perturbations in membrane structure and to protect against oxidative attack. In addition, the effect of fat-soluble antioxidants on lipid peroxidation was observed in the high-dose (200 ng/L)-treated group, resulting in a change in the lipid profile disproportionate to the dose. This study concludes that coral accommodates the membrane lipid profile of symbiosomes to herbicide-induced photoinhibition dependent on the duration of exposure.
- Published
- 2021
38. Membrane lipid profiles of coral responded to zinc oxide nanoparticle-induced perturbations on the cellular membrane
- Author
-
Wei-Hsien Wang, Shu-Hui Lee, Chuan-Ho Tang, and Ching-Yu Lin
- Subjects
0301 basic medicine ,Health, Toxicology and Mutagenesis ,Membrane lipids ,Coral ,Cell ,Taiwan ,Phospholipid ,chemistry.chemical_element ,Seriatopora caliendrum ,Zinc ,010501 environmental sciences ,Aquatic Science ,Biology ,01 natural sciences ,Membrane Lipids ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Animals ,Lipid bilayer ,0105 earth and related environmental sciences ,Cell Membrane ,fungi ,technology, industry, and agriculture ,Anthozoa ,030104 developmental biology ,Membrane ,medicine.anatomical_structure ,Biochemistry ,chemistry ,Biophysics ,Nanoparticles ,Zinc Oxide ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Zinc oxide nanoparticles (nZnOs) released from popular sunscreens used during marine recreation apparently endanger corals; however, the known biological effects are very limited. Membrane lipids constitute the basic structural element to create cell a dynamic structure according to the circumstance. Nano-specific effects have been shown to mechanically perturb the physical state of the lipid membrane, and the cells accommodating the actions of nZnOs can be involved in the alteration of the membrane lipid composition. To gain insight into the effects of nanoparticles on coral, glycerophosphocholine (GPC) profiling of the coral Seriatopora caliendrum exposed to nZnOs was performed in this study. Increasing lyso-GPCs, docosapentaenoic acid-possessing GPCs and docosahexaenoic acid-possessing GPCs and decreasing arachidonic acid-possessing GPCs were the predominant changes responded to nZnO exposure in the coral. A backfilling of polyunsaturated plasmanylcholines was observed in the coral exposed to nZnO levels over a threshold. These changes can be logically interpreted as an accommodation to nZnOs-induced mechanical disturbances in the cellular membrane based on the biophysical properties of the lipids. Moreover, the coral demonstrated a difference in the changes in lipid profiles between intra-colonial functionally differentiated polyps, indicating an initial membrane composition-dependent response. Based on the physicochemical properties and physiological functions of these changed lipids, some chronic biological effects can be incubated once the coral receives long-term exposure to nZnOs.
- Published
- 2017
39. An environmentally friendly strategy for determining organic ultraviolet filters in seawater using liquid-phase microextraction with liquid chromatography-tandem mass spectrometry
- Author
-
Te-An Kung, Ting-Yu Liu, Ping-Chang Ku, Wei-Hsien Wang, and Shu Hui Lee
- Subjects
Liquid Phase Microextraction ,Health, Toxicology and Mutagenesis ,Taiwan ,010501 environmental sciences ,Tandem mass spectrometry ,Mass spectrometry ,medicine.disease_cause ,01 natural sciences ,Gas Chromatography-Mass Spectrometry ,Liquid chromatography–mass spectrometry ,Tandem Mass Spectrometry ,medicine ,Environmental Chemistry ,Sample preparation ,Seawater ,0105 earth and related environmental sciences ,Chromatography ,Chemistry ,Extraction (chemistry) ,General Medicine ,Pollution ,Solvent ,Sunscreening Agents ,Ultraviolet ,Water Pollutants, Chemical ,Chromatography, Liquid - Abstract
Benzophenone-3, benzophenone-8, and 4-methylbenzylidene camphor are used in sunscreens because they can protect the skin from UV radiation. The widespread use of organic UV filters may mean that they directly or indirectly enter seawater during recreational activities or through sewage discharge. In this study, a simple and efficient method using 1-octanol:isooctane (2:8, v/v) as an extraction solvent and liquid chromatography–electrospray tandem mass spectrometry was developed to measure trace levels of organic UV filters in seawater samples. This proposed method proved to be a highly sensitive, low-cost, and green analytical tool that requires minimal sample preparation. The method was validated and it exhibited favorable performance as well as acceptable accuracy (67 to 115%), precision (2.1 to 7.3%), coefficients of determination (0.9952
- Published
- 2019
40. Rumphellolide K, a Novel C-3/8 Ether Linkage Caryophyllane from Rumphella antipathes
- Author
-
Wei Hsien Wang, Ping-Jyun Sung, Jih Jung Chen, Tsong-Long Hwang, Hsu Ming Chung, and Yu Ming Chang
- Subjects
Pharmacology ,Linkage (software) ,chemistry.chemical_compound ,biology ,chemistry ,Stereochemistry ,Organic Chemistry ,Antipathes ,Ether ,biology.organism_classification ,Analytical Chemistry - Published
- 2020
41. Lipidomics as a diagnostic tool of the metabolic and physiological state of managed whales: A correlation study of systemic metabolism
- Author
-
Wei-Hsien Wang, Ching-Yu Lin, Yi-Lun Tsai, Chuan-Ho Tang, and Shu-Hui Lee
- Subjects
0301 basic medicine ,Physiology ,Context (language use) ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Lipidomics ,medicine ,Blood test ,Animals ,Animal Husbandry ,Mean corpuscular volume ,medicine.diagnostic_test ,Cholesterol ,Whales ,General Medicine ,Metabolism ,Lipid Metabolism ,Lipids ,030104 developmental biology ,chemistry ,Beluga Whale ,lipids (amino acids, peptides, and proteins) ,Animal Science and Zoology ,Animals, Zoo ,Lipid profile ,030217 neurology & neurosurgery - Abstract
Integrating multifactor blood analysis is a key step toward a precise diagnosis of the health status of marine mammals. Variations in the circulating lipid profile reflect changes in the metabolism and physiology of an individual. To demonstrate the practicability of lipid profiling for physiological assessment, the phosphorylcholine-containing lipids in the plasma of long-term managed beluga whales (Delphinapterus leucas) were profiled using a lipidomics methodology. Using a multivariate analysis, the mean corpuscular volume, cholesterol, potassium, and γ-glutamyltranspeptidase levels were well modeled with the lipid profile of the female whales. In the models, the correlated lipids provided information about blood parameter-related metabolism and physiological regulation, in particular relating to cholesterol and inflammation. In the males, the levels of cholesterol, triglycerides, blood urea nitrogen, creatinine, plasma iron, and segmented neutrophil were well modeled with the lipid profile. In addition to providing information about the related metabolism and regulation, through a cross-linked analysis of the blood parameters, the correlated lipids indicated a parallel regulation involved in the energy metabolism of the male whales. Lipidomics as a method for revealing the context of physiological change shows practical potential for the health care of managed whales.
- Published
- 2018
42. Survey of selected personal care products in surface water of coral reefs in Kenting National Park, Taiwan
- Author
-
Ting Chi Yang, Shu Hui Lee, Wei Hsien Wang, and Te An Kung
- Subjects
Environmental Engineering ,Parks, Recreational ,Taiwan ,Sewage ,Parabens ,010501 environmental sciences ,01 natural sciences ,chemistry.chemical_compound ,Benzophenones ,Environmental Chemistry ,Seawater ,Waste Management and Disposal ,0105 earth and related environmental sciences ,geography ,geography.geographical_feature_category ,National park ,business.industry ,Coral Reefs ,010401 analytical chemistry ,Estuary ,Coral reef ,Pollution ,Triclosan ,0104 chemical sciences ,Nonylphenol ,chemistry ,Environmental chemistry ,Environmental science ,business ,Surface water ,Propylparaben ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Kenting National Park (KNP) located in the Hengchun Peninsula in southern Taiwan is a popular tourist spot, annually attracting millions of visitors, who engage in water sport and amusement activities. In this region, sewage is directly discharged into the marine environment. In this study, the concentrations of five organic UV filters [benzophenone (BP), 2,4-dihydroxy benzophenone (BP-1), 2-hydroxy-4-methoxy benzophenone (BP-3), 2,2′-dihydroxy-4-methoxy benzophenone (BP-8), and 4-methylbenzylidene camphor], five preservatives [methylparaben (MeP), ethylparaben, propylparaben (PrP), butylparaben, and benzylparaben], one disinfectant [triclosan (TCS)], and twenty-four detergent derivatives [nonylphenol (NP), nonylphenol ethoxylates (NP2EO–NP12EO), octylphenol (OP) and octylphenol ethoxylates OP2EO–OP12EO] were detected in seawater and river water samples collected from eight beaches in KNP and two major river estuaries in the Hengchun Peninsula. BP-3 was detected at all sampling sites and was higher in concentration than the other organic UV filters. The highest concentration of BP-3 was 1233 ng/L collected from Wanlitong Beach. MeP and PrP were the main preservative components in seawater. The highest total content of preservative agents was 164 ng/L collected from Houwan Beach. Moreover, NP was detected at all sampling sites, with the highest concentration found at Sail Rock Beach (26.5 ng/L). The highest concentration of OP was 113 ng/L in the Boli River estuary. The widespread use of personal care products (PCPs) has resulted in the release of their major ingredients into natural ecosystems. Therefore, the potential long-term effects of multi-PCPs at low concentration exposure to on the coral reef ecosystem in KNP must be considered and monitored.
- Published
- 2018
43. Persistent organic pollutants in Antarctic notothenioid fish and invertebrates associated with trophic levels
- Author
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Jing-O Cheng, Li-Sing Fang, Chih-Wei Chang, Te-Hao Chen, Shu-Ji Kao, Fung-Chi Ko, Wei-Hsien Wang, Wei-Ling Pan, Hsuan-Ching Ho, and Fu-Wen Kuo
- Subjects
Composite Particles ,010504 meteorology & atmospheric sciences ,genetic structures ,Biomagnification ,lcsh:Medicine ,Bioconcentration ,010501 environmental sciences ,01 natural sciences ,Geographical Locations ,Food chain ,Isotopes ,Contaminants ,Hydrocarbons, Chlorinated ,Food Chains ,Tissue Distribution ,Organic Chemicals ,lcsh:Science ,Trophic level ,Carbon Isotopes ,Multidisciplinary ,Ecology ,biology ,Physics ,Stable Isotopes ,Fishes ,Eukaryota ,Biota ,Polychlorinated Biphenyls ,Chemistry ,Bioaccumulation ,Environmental chemistry ,Physical Sciences ,Environmental Pollutants ,Chlorine ,Environmental Monitoring ,Research Article ,Pollutants ,Atoms ,Food Chain ,Krill ,Fish Biology ,Materials Science ,Antarctic Regions ,Fish Physiology ,Animals ,Environmental Chemistry ,Animal Physiology ,Pesticides ,Particle Physics ,Ecosystem ,Materials by Attribute ,0105 earth and related environmental sciences ,Pollutant ,Nitrogen Isotopes ,Ecology and Environmental Sciences ,fungi ,lcsh:R ,Organisms ,Biology and Life Sciences ,biology.organism_classification ,Invertebrates ,Vertebrate Physiology ,Diet ,People and Places ,Antarctica ,Environmental science ,lcsh:Q ,Zoology ,Water Pollutants, Chemical - Abstract
Notothenioid fish and invertebrate samples from Antarctica were collected in the austral summer of 2009, and analyzed for persistent organic pollutants (POPs), including polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (OCPs), and polybrominated diphenylethers (PBDEs), as well as δ13C and δ15N stable isotopes for trophic level determination. In this study, the POP levels in the Antarctic biota samples were found to be ranked in the following order: OCPs > PAHs >> PBDEs. The POP levels in notothenioid fish and krill correlate to trophic levels; however, the POP concentrations in intertidal benthic invertebrates are higher than in notothenioid fish implying that specific biogeochemical factors may affect bioaccumulation in the Antarctica ecosystem. Biomagnification of POPs may have a smaller role than bioconcentration in Antarctica environment. In addition to the source, transport, exposure, and absorption for each group of POPs in the short food chain in Antarctica, the biological variation among species, interaction habitats, diet and metabolism are also factors for future studies on contaminant bioaccumulation.
- Published
- 2018
44. Intra-Colonial Functional Differentiation-Related Modulation of the Cellular Membrane in a Pocilloporid Coral Seriatopora caliendrum
- Author
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Shu-Hui Lee, Wei-Hsien Wang, Ching-Yu Lin, Chuan-Ho Tang, Kuo-Hsin Lee, Te-Hao Chen, and Ping-Chang Ku
- Subjects
biology ,Membrane lipids ,Coral ,Cell Membrane ,Phospholipid ,Seriatopora caliendrum ,Anthozoa ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Phenotype ,Cell membrane ,chemistry.chemical_compound ,medicine.anatomical_structure ,Membrane ,Biochemistry ,chemistry ,Phosphatidylcholines ,medicine ,Animals - Abstract
Scleractinian corals have displayed phenotypic gradients of polyps within a single genotypic colony, and this has profound implications for their biology. The intrinsic polymorphism of membrane lipids and the molecular interactions involved allow cells to dynamically organize their membranes to have physicochemical properties appropriate for their physiological requirements. To gain insight into the accommodation of the cellular membrane during ontogenetic shifts, intra-colony differences in the glycerophosphocholine profiling of a pocilloporid coral, Seriatopora caliendrum, were characterized using a previously validated method. Specifically, several major polyunsaturated phosphatidylcholines showed higher levels in the distal tissue of coral branches. In contrast, the corresponding molecules with 1-2-degree less unsaturation and plasmanylcholines were expressed more highly in the proximal tissue. The lipid profiles of these two colonial positions also contrasted sharply with regard to the saturated, monounsaturated, and lyso-glycerophosphocholine ratios. Based on the biochemical and biophysical properties of these lipids, the associated modulation of cellular membrane properties could be related to the physiological requirements, including coral growth and aging, of the functionally differentiated polyps. In this study, the metabolic regulation of membrane lipids involved in the functional differentiation of polyps within a S. caliendrum colony was identified.
- Published
- 2015
45. A generic and rapid strategy for determining trace multiresidues of sulfonamides in aquatic products by using an improved QuEChERS method and liquid chromatography–electrospray quadrupole tandem mass spectrometry
- Author
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Wei-Hsien Wang, Te-An Kung, Chung-Wei Tsai, and Bing Chang Ku
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Electrospray ,Formic acid ,Analytical chemistry ,Chemical Fractionation ,Tandem mass spectrometry ,Quechers ,Sensitivity and Specificity ,Analytical Chemistry ,chemistry.chemical_compound ,Sulfanilamide ,Limit of Detection ,Tandem Mass Spectrometry ,Sulfanilamides ,Animals ,Sample preparation ,Chromatography, High Pressure Liquid ,Detection limit ,Sulfonamides ,Chromatography ,Reproducibility of Results ,General Medicine ,Drug Residues ,chemistry ,Methanol ,Ammonium acetate ,Food Science - Abstract
A rapid and efficient multiresidue method that involves using improved QuEChERS method and liquid chromatography–electrospray tandem mass spectrometry (LC–ESI–MS/MS) was developed to measure trace levels of sulfonamides in fish tissue. This proposed method was proven to be a powerful, highly sensitive, and environmentally friendly analytical tool that requires minimal sample preparation. The typical MS/MS fragmentation patterns of the [M+H] + were 156 m/z, 108 m/z, and 92 m/z. Separation was performed on HC-C18 columns with a gradient elution by using methanol −5 mM ammonium acetate containing formic acid (pH 3.5). This method was validated and exhibited favorable performance as well as acceptable accuracy (80.2–93.5%), precision (3.82–8.71%), sensitivity (limits of detection (LODs) 0.43–1.22 μg kg −1 , limits of quantification (LOQs) 1.27–3.71 μg kg −1 ; decision limit (CC α ) 1.49–10.9 μg kg −1 , detection capability (CC β ) 1.71–11.4 μg kg −1 ), and an acceptable matrix effect (−18.2–18.4%). This methodology has been successfully applied in analyzing various fish tissue from local markets.
- Published
- 2015
46. Norcembranoidal Diterpenes from the Cultured-Type Octocoral Sinularia numerosa
- Author
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Ping-Jyun Sung, Yang Chang Wu, Wei Hsien Wang, Mei-Chin Lu, Ching Hsiao Cheng, Chen Ting Yin, Zhi-Hong Wen, Wu Fu Chen, Lee Shing Fang, and Jih Jung Chen
- Subjects
Stereochemistry ,Cell Survival ,Tumor cells ,Biology ,Catalysis ,Article ,lcsh:Chemistry ,Inorganic Chemistry ,chemistry.chemical_compound ,Sinularia numerosa ,Cell Line, Tumor ,Sinuleptolide ,sinuleptolide ,Structure–activity relationship ,Animals ,Physical and Theoretical Chemistry ,Cytotoxicity ,lcsh:QH301-705.5 ,Molecular Biology ,IC50 ,Nuclear Magnetic Resonance, Biomolecular ,Spectroscopy ,Cell survival ,Molecular Structure ,Organic Chemistry ,General Medicine ,Anthozoa ,Computer Science Applications ,lcsh:Biology (General) ,lcsh:QD1-999 ,chemistry ,cembranoidal diterpene ,cytotoxicity ,Diterpene ,Diterpenes - Abstract
A known norcembranoidal diterpene, 5-episinuleptolide (1), along with a new analogue, 4α-hydroxy-5-episinuleptolide (2), were isolated from a cultured-type soft coral Sinularia numerosa. The structures of 1 and 2 were elucidated on the basis of spectroscopic methods and by comparison of the data with those of the related metabolites. Cytotoxicity of metabolites 1 and 2 against a panel of tumor cells is also described. Compound 2 exhibited moderate cytotoxicity toward CCRF-CEM cells with an IC50 value 4.21 μg/mL. Preliminary SAR (structure activity relationship) information was obtained from these two compounds.
- Published
- 2015
47. Rumphellaoic Acid A, a Novel Sesquiterpenoid from the Formosan Gorgonian Coral Rumphella antipathies
- Author
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Jih Jung Chen, Lee Shing Fang, Wei Hsien Wang, Hsu Ming Chung, Ping-Jyun Sung, Yang Chang Wu, Zhi-Hong Wen, and Tsong-Long Hwang
- Subjects
Stereochemistry ,Neutrophils ,Coral ,gorgonian ,Carbon skeleton ,Taiwan ,Pharmaceutical Science ,Rumphella antipathies ,Biology ,Anthozoa ,Drug Discovery ,Botany ,Animals ,Humans ,elastase ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Inhibitory effect ,Pancreatic elastase ,lcsh:QH301-705.5 ,Pancreatic Elastase ,Communication ,Spectrum Analysis ,Elastase ,sesquiterpenoid ,biology.organism_classification ,Gorgonian ,rumphellaoic acid ,lcsh:Biology (General) ,Spectrum analysis ,Sesquiterpenes - Abstract
A novel sesquiterpenoid, rumphellaoic acid A (1), was isolated from the gorgonian coral Rumphella antipathies, and was found to possess a carbon skeleton that was obtained for the first time from a natural sources. The structure of 1 was elucidated by spectroscopic methods and this compound and was found to exert a moderate inhibitory effect on the release of elastase by human neutrophils.
- Published
- 2014
48. Cytotoxic Polyacetylenes from a Formosan Marine Sponge Callyspongia sp
- Author
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Huey-Jen Su, Jyh-Horng Sheu, Jui‐Hsin Su, Wei-Hsien Wang, Mei-Chin Lu, and Ching-Wen Chiu
- Subjects
Sponge ,biology ,Stereochemistry ,Callyspongia ,Chemistry ,General Chemistry ,biology.organism_classification - Abstract
Two new polyacetylenic compounds, callysponynes A (1) and B (2), were isolated from the marine sponge Callyspongia sp. The structures of the new metabolites were elucidated on the basis of their sp...
- Published
- 2014
49. Rumphellols A and B, New Caryophyllene Sesquiterpenoids from a Formosan Gorgonian Coral, Rumphella antipathies
- Author
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Yang Chang Wu, Wei Hsien Wang, Zhi-Hong Wen, Ping-Jyun Sung, Tsong-Long Hwang, Jih Jung Chen, Yu Bao Wang, Hsu Ming Chung, and Lee Shing Fang
- Subjects
Cell Extracts ,sesquiterpene ,Sesterterpenes ,rumphellol ,Neutrophils ,Stereochemistry ,Coral ,Rumphella antipathies ,Sesquiterpene ,Article ,Catalysis ,caryophyllene ,superoxide anion ,elastase ,Inorganic Chemistry ,lcsh:Chemistry ,chemistry.chemical_compound ,Superoxides ,Anthozoa ,Animals ,Humans ,Physical and Theoretical Chemistry ,Molecular Biology ,lcsh:QH301-705.5 ,Spectroscopy ,Polycyclic Sesquiterpenes ,Pancreatic Elastase ,biology ,Caryophyllene ,Anti-Inflammatory Agents, Non-Steroidal ,Organic Chemistry ,General Medicine ,biology.organism_classification ,Computer Science Applications ,Gorgonian ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,Sesquiterpenes - Abstract
Two new marine-derived caryophyllene-type sesquiterpenoids, rumphellols A and B (1 and 2), were obtained from the gorgonian coral, Rumphella antipathies, collected off the waters of Taiwan. Although caryophyllene-type sesquiterpenes are rarely found in marine organisms, compounds of this type could be principal components of R. antipathies. The structures of new Compounds 1 and 2 were determined by analysis of their spectroscopic data, including 1D and 2D NMR experiments. Caryophyllene 1 and 2 were evaluated in terms of their anti-inflammatory activity by examining their inhibitory effects on the generation of superoxide anions and the release of elastase by human neutrophils.
- Published
- 2014
50. Anti-Inflammatory Effect of Momordica Charantia in Sepsis Mice
- Author
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Yueh-Hsiung Kuo, Ping-Jyun Sung, Che-Yi Chao, and Wei-Hsien Wang
- Subjects
Lipopolysaccharides ,Male ,medicine.medical_specialty ,LPS ,Momordica charantia ,PPARs ,medicine.drug_class ,medicine.medical_treatment ,Intraperitoneal injection ,Anti-Inflammatory Agents ,Drug Evaluation, Preclinical ,Bitter gourd ,Pharmaceutical Science ,Blood lipids ,Inflammation ,sepsis ,anti-inflammation ,Article ,Anti-inflammatory ,Analytical Chemistry ,Sepsis ,lcsh:QD241-441 ,NEFA ,lcsh:Organic chemistry ,Internal medicine ,Drug Discovery ,medicine ,Animals ,Physical and Theoretical Chemistry ,Mice, Inbred BALB C ,Plant Extracts ,business.industry ,Organic Chemistry ,medicine.disease ,Cytokine ,Endocrinology ,Chemistry (miscellaneous) ,Immunology ,Cytokines ,Molecular Medicine ,Inflammation Mediators ,medicine.symptom ,business ,Spleen - Abstract
Wild bitter gourd (Momordica charantia L. var. abbreviate Seringe), a common vegetable in Asia, is used in traditional medicine to treat various diseases, including inflammation. Extant literature indicates that wild bitter gourds have components that activate PPARα and PPARγ. This research probed the influence of adding wild bitter gourd to diets on inflammation responses in mice with sepsis induced by intraperitoneal injection of LPS. Male BALB/c mice were divided normal, sepsis, positive control, and three experimental groups. The latter ate diets with low (1%), moderate (2%), and high (10%) ratios of wild bitter gourd lyophilized powder. Before mice were sacrificed, with the exception of the normal group, intraperitoneal injection of LPS induced sepsis in each group; positive control group was injected with LPS after PDTC. This experiment revealed starkly lower weights in groups with added wild bitter gourd than those of the remaining groups. Blood lipids (TG, cholesterol, and NEFA) were also lower in comparison to the sepsis group, and blood glucose concentrations recovered and approached normal levels. Blood biochemistry values related to inflammation reactions indicated GOT, GPT, C-RP, and NO concentrations of groups with added wild bitter gourd were all lower than those of the sepsis group. Secretion levels of the spleen pro-inflammatory cytokines IL-1, IL-6, and TNF-α tallied significantly lower in comparison to the sepsis group, whereas secretion levels of IL-10 anti-inflammatory cytokine increased. Expression level of proteins NF-κB, iNOS, and COX-2 were significantly inhibited. Results indicate wild bitter gourd in diets promoted lipid metabolism, reducing fat accumulation, and improving low blood glucose in sepsis. Addition of wild bitter gourd can reduce inflammation biochemical markers or indicators and pro-inflammatory cytokines in the body, hence improving the inflammation responses in mice with sepsis.
- Published
- 2014
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