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Your search keyword '"Xihui Zhu"' showing total 6 results
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6 results on '"Xihui Zhu"'

1. Isolation and identification of the broad-spectrum high-efficiency phage vB_SalP_LDW16 and its therapeutic application in chickens

Author

Shengliang Cao, Wenwen Yang, Xihui Zhu, Cheng Liu, Jianbiao Lu, Zhenshu Si, Lanying Pei, Leilei Zhang, Wensi Hu, Yanlan Li, Zhiwei Wang, Zheyu Pang, Xijuan Xue, and Yubao Li

Subjects
Salmonella, Phages vB_SalP_LDW16, Biological characteristics, Recovery rate, Phage therapy, Veterinary medicine, SF600-1100
Abstract

Abstract Background Salmonella infection in livestock and poultry causes salmonellosis, and is mainly treated using antibiotics. However, the misuse use of antibiotics often triggers the emergence of multi-drug-resistant Salmonella strains. Currently, Salmonella phages is safe and effective against Salmonella, serving as the best drug of choice. This study involved 16 Salmonella bacteriophages separated and purified from the sewage and the feces of the broiler farm. A phage, vB_SalP_LDW16, was selected based on the phage host range test. The phage vB_SalP_LDW16 was characterized by the double-layer plate method and transmission electron microscopy. Furthermore, the clinical therapeutic effect of phage vB_SalP_LDW16 was verified by using the pathogenic Salmonella Enteritidis in the SPF chicken model. Results The phage vB_SalP_LDW16 with a wide host range was identified to the family Siphoviridae and the order Caudoviridae, possess a double-stranded DNA and can lyse 88% (22/25) of Salmonella strains stored in the laboratory. Analysis of the biological characteristics, in addition, revealed the optimal multiplicity of infection (MOI) of vB_SalP_LDW16 to be 0.01 and the phage titer to be up to 3 × 1014 PFU/mL. Meanwhile, the phage vB_SalP_LDW16 was found to have some temperature tolerance, while the titer decreases rapidly above 60 ℃, and a wide pH (i.e., 5–12) range as well as relative stability in pH tolerance. The latent period of phage was 10 min, the burst period was 60 min, and the burst size was 110 PFU/cell. Furthermore, gastric juice was also found to highly influence the activity of the phage. The clinical treatment experiments showed that phage vB_SalP_LDW16 was able to significantly reduce the bacterial load in the blood through phage treatment, thereby improving the pathological changes in the intestinal, liver, and heart damage, and promoting the growth and development of the chicken. Conclusions The phage vB_SalP_LDW16 is a highly lytic phage with a wide host range, which can be potentially used for preventing and treating chicken salmonellosis, as an alternative or complementary antibiotic treatment in livestock farming.

Published
2022
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4. Combining curcumin and aspirin ameliorates preeclampsia-like symptoms by inhibiting the placental TLR4/NF-κB signaling pathway in rats

Author

Yaru Ju, Yan Feng, Yanjing Yang, Xiaolin Hou, Xiaofeng Zhang, Xihui Zhu, Yage Wang, and Meiliu Yang

Subjects
Obstetrics and Gynecology
Abstract

Preeclampsia (PE) is a common medical complication of pregnancy characterized by high blood pressure and proteinuria after the 20th gestational week. This study aimed to investigate the potency of the combination of curcumin and aspirin in the treatment of PE and explore the underlying mechanisms.The PE model was constructed in female rats by administering 0.5 mg/mL N-nitro-L-arginine methyl ester from gestational days (GDs) 6 to 16. The pregnant female rats were divided into five groups according to the drug treatment. The curcumin or aspirin was given to the rats by tail vein injection (0.36 mg/kg) or gavage treatment (1.5 mg/kg BW/day) from GD4 to GD18.Treatment with curcumin and aspirin combination significantly reduced the systolic blood pressure and proteinuria in the PE rats. Meanwhile, in comparison to the PE rats treated with single-dose curcumin or aspirin, the rats treated with combined curcumin and aspirin showed significantly decreased sFlt-1, increased placental growth factor, and alleviated oxidative stress in both blood and placental tissues, which are abnormal in no-treated PE rats. Furthermore, dramatically decreased inflammatory cytokines secretion and TLR4 and NF-κB p65 expression in placental tissues were also observed in the PE rats with combined treatment compared to those of no-treated, signal-dose curcumin or aspirin-treated PE rats.Our results suggested that the combined treatment of curcumin and aspirin significantly ameliorates the symptoms of PE in rats, which is most likely due to the inhibition of the placental TLR4/NF-κB p65 signaling pathway.

Published
2022

5. A Case of Placental Abruption Complicated by Vaginal Trial of Labor in a Unicornuate Uterus

Author

Xiao Chen, Chunhui Xiao, Xueling Zhang, Yu Liang, Xihui Zhu, Yingfang Gao, and Zhexia Hu

Abstract

Unicornuate uterus has a low incidence,it is a congenital uterine developmental anomaly, it has many complications during pregnancy, and it is less common in pregnancy to term. This article retrospectively analyzes a case of unicornuate uterus with a full-term vaginal trial of labor and delivery with placental abruption resulting in neonatal asphyxia. We also present the typology of unicornuate uterus, diagnosis, and discuss the mode of delivery in the light of the literature, in order to raise awareness of this type of disease among medical professionals and reduce the occurrence of adverse pregnancy outcomes.

Published
2021

6. Antibiotic resistance of Riemerella anatipestiferand comparative analysis of antibiotic-resistance gene detection methods

Author

Xihui, Zhu, Yanlan, Li, Zhiwei, Wang, Zheyu, Pang, Zhenshu, Si, Cheng, Liu, Jianbiao, Lu, Shengliang, Cao, Lanying, Pei, and Yubao, Li

Abstract

Riemerella anatipestiferis an important pathogen in waterfowl, and is generally multidrug resistant. This study assessed the current status of Riemerella anatipestiferantibiotic resistance and antibiotic-resistance genes (ARGs), compared the results of different detection methods, and evaluated a new method of studying the association between antibiotic resistance and ARGs in Riemerella anatipestifer. In this study, 51 strains of Riemerella anatipestiferwere isolated from ducks on several farms, their resistance to 28 antibiotics was assessed, and the isolates were subjected to whole-genome sequencing. The number of ARGs carried by Riemerella anatipestiferwas predicted, compared, and analyzed, and the consistency between ARGs and antibiotic-resistance phenotypes was assessed. The potential for loss of resistance genes during the sequencing and assembly of genomic framework maps was assessed, and a new ARG detection method was pilot tested. The 51 strains of Riemerella anatipestiferwere multidrug resistant (MDR) and had high level of resistance to aminoglycosides, trimethoprim, lincosamides, polypeptides, and macrolides. Based on the genome-wide framework map of the 51 strains, 3 databases of ABRicate software were used to detect ARGs, and an mean of 4 to 5 ARGs were identified per isolate. Although the detection results differed according to the database used, the general performance was consistent. The online website detected more types of ARG than the bioinformatics software. The association between ARGs and antibiotic-resistance phenotypes was assessed, and the ermFgene was identified as a possible key ARG regulating macrolide resistance of Riemerella anatipestifer. The method used to investigate and detect Riemerella anatipestiferARGs was convenient and rapid, and had strong accuracy and pertinence. The ARG detection method reported here combined the advantages of PCR and genome detection, and could greatly reduce workload and detect ARGs more precisely.

Published
2022
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