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BACKGROUND: Ileal neuroendocrine tumors (i-NETs) are characterized by their multifocality and bulky mesenteric mass. Having shown that minimally invasive surgery (MIS) utilizing a hand-access port device has favorable short-term outcomes and achieves the goals of surgery for i-NETs, we sought to analyze long-term survival outcomes of MIS. METHODS: One hundred and sixty-eight patients who underwent resection of primary i-NETs at a single institution between January 2007 and February 2023 were retrospectively studied. Patients were categorized into the MIS or open surgery cohorts on an intention-to-treat basis. Open surgery was selected mainly based on the need for hepatectomy or bulky mesenteric mass resection. Overall survival was analyzed using log-rank tests with propensity score matching (PSM) and Cox proportional hazards regression. PSM was performed to reduce standardized mean differences of the variables to

Abstract Laser-driven neutron sources (LDNSs) can generate strong short-pulse neutron beams, which are valuable for scientific studies and engineering applications. Neutron resonance transmission analysis (NRTA) is a nondestructive technique used for determining the areal density of each nuclide in a material sample using pulsed thermal and epithermal neutrons. Herein, we report the first successful NRTA performed using an LDNS driven by the Laser for Fast Ignition Experiment at the Institute of Laser Engineering, Osaka University. The key challenge was achieving a well-resolved resonance transmission spectrum for material analysis using an LDNS with a limited number of laser shots in the presence of strong background noise. We addressed this by employing a time-gated $$^{6}{\textrm{Li}}$$ 6 Li -glass scintillation neutron detector to measure the transmission spectra, reducing the impact of electromagnetic noise and neutron and gamma-ray flashes. Output waveforms were recorded for each laser shot and analyzed offline using a counting method. This approach yielded a spectrum with distinct resonances, which were attributed to $$^{115}\,{\textrm{In}}$$ 115 In and $$^{109}{\textrm{Ag}}$$ 109 Ag , as confirmed through neutron transmission simulation. The spectrum was analyzed using the least-square nuclear-resonance fitting program, REFIT, demonstrating the possibility of using an LDNS for nondestructive areal-density material characterization.

Abstract DNA hypomethylating agents (HMAs) are used for the treatment of myeloid malignancies, although their therapeutic effects have been unsatisfactory. Here we show that CRISPR-Cas9 screening reveals that knockout of topoisomerase 1-binding arginine/serine-rich protein (TOPORS), which encodes a ubiquitin/SUMO E3 ligase, augments the efficacy of HMAs on myeloid leukemic cells with little effect on normal hematopoiesis, suggesting that TOPORS is involved in resistance to HMAs. HMAs are incorporated into the DNA and trap DNA methyltransferase-1 (DNMT1) to form DNA-DNMT1 crosslinks, which undergo SUMOylation, followed by proteasomal degradation. Persistent crosslinking is cytotoxic. The TOPORS RING finger domain, which mediates ubiquitination, is responsible for HMA resistance. In TOPORS knockout cells, DNMT1 is stabilized by HMA treatment due to inefficient ubiquitination, resulting in the accumulation of unresolved SUMOylated DNMT1. This indicates that TOPORS ubiquitinates SUMOylated DNMT1, thereby promoting the resolution of DNA-DNMT1 crosslinks. Consistently, the ubiquitination inhibitor, TAK-243, and the SUMOylation inhibitor, TAK-981, show synergistic effects with HMAs through DNMT1 stabilization. Our study provides a novel HMA-based therapeutic strategy that interferes with the resolution of DNA-DNMT1 crosslinks.

Abstract The temperature measurement of material inside of an object is one of the key technologies for control of dynamical processes. For this purpose, various techniques such as laser-based thermography and phase-contrast imaging thermography have been studied. However, it is, in principle, impossible to measure the temperature of an element inside of an object using these techniques. One of the possible solutions is measurements of Doppler brooding effect in neutron resonance absorption (NRA). Here we present a method to measure the temperature of an element or an isotope inside of an object using NRA with a single neutron pulse of approximately 100 ns width provided from a high-power laser. We demonstrate temperature measurements of a tantalum (Ta) metallic foil heated from the room temperature up to 617 K. Although the neutron energy resolution is fluctuated from shot to shot, we obtain the temperature dependence of resonance Doppler broadening using a reference of a silver (Ag) foil kept to the room temperature. A free gas model well reproduces the results. This method enables element(isotope)-sensitive thermometry to detect the instantaneous temperature rise in dynamical processes.

Abstract Background We aimed to aid the appropriate use of antimicrobial agents by determining the timing of secondary bacteremia and validating and updating clinical prediction models for bacteremia in patients with COVID‐19. Methods We performed a retrospective cohort study on all hospitalized patients diagnosed with COVID‐19 who underwent blood culture tests from January 1, 2020, and September 30, 2021, at an urban teaching hospital in Japan. The primary outcome measure was secondary bacteremia in patients with COVID‐19. Results Of the 507 patients hospitalized with COVID‐19, 169 underwent blood culture tests. Eleven of them had secondary bacteremia. The majority of secondary bacteremia occurred on or later than the 9th day after symptom onset. Positive blood culture samples collected on day 9 or later after disease onset had an odds ratio of 22.4 (95% CI 2.76–181.2, p

Chemistry students still have low levels of scientific literacy, although in the era of the Industrial Revolution 4.0 and the transition to Society 5.0, students need these skills. An alternative for raising students' scientific literacy and character is the Culturally Wetland Responsive Teaching (CWRT) approach. This developmental research tested the validity, practicality, and effectiveness of e-modules based on CWRT for the colligative properties of solutions. The ADDIE development model was used for the research method. Both test and non-test instruments were used for data collection. The e-module was tested on 23 students from grades A1 and A2 in the Chemistry Education Study Program at a public university in South Kalimantan, Indonesia. The analysis showed that the e-modules were highly valid regarding content, presentation, language, and media feasibility. They were also highly practical in individual and small group tests, limited trials, and assessing lecturers' ability to use e-modules and manage classes. The modules were effective, as evidenced by the N-gain value of science literacy competence and the wasaka character of students in the high category. The results suggest that e-modules based on Culturally Wetland Responsive Teaching are valid, practical, and effective for teaching the colligative properties of solutions.

Oxidative stress and inflammation play a central role in the progression of nonalcoholic steatohepatitis (NASH), which can lead to liver cirrhosis. Barley bran has potential bioactivities due to its high content of functional substances, such as anthocyanins, with anti-inflammatory and anti-oxidative properties. Here, we investigated whether barley bran polyphenol-rich extracts (BP) can prevent NASH in Sprague–Dawley rats fed a high-fat and high-cholesterol diet including 1.25% or 2.5% cholesterol for 9 weeks. In the rat model of NASH with advanced hepatic fibrosis, BP prevented NASH development by ameliorating the histopathological findings of lobular inflammation. The BP also tended to attenuate serum aspartate aminotransferase level in this model. In the rat model of NASH with mild-to-moderate hepatic fibrosis, BP tended to attenuate the serum levels of transaminases. BP-dose-dependent effects were revealed for several parameters, including monocyte chemoattractant protein-1, transforming growth factor-β, and manganese superoxide dismutase gene expressions in the liver. These results suggest that BP may prevent NASH development or progression, presumably due to its anti-inflammatory and anti-oxidative properties.

Abstract We aimed to investigate whether ventilator support time influences the occurrence of dysphagia in pediatric trauma patients. This case-series study was conducted in a single pediatric emergency and critical care center from April 2012 to March 2022. Trauma patients aged

Oryctes rhinoceros is an important pest of various coconut and palm species. Studies on the bacterial gut microbiome of O. rhinoceros larvae and their potential use as producers of industrial enzymes are still limited. This study aims to examine the diversity and abundance of culturable bacterial microbiome in the gut of O. rhinoceros and their potential to produce protease and lipase. This study consisted of several stages, i.e., collection of O. rhinoceros larvae from coconut plantations; isolation of culturable bacteria from larval gut; protease and lipase enzyme activity assays; and partial characterization of culturable gut bacteria in terms of morphology, physiology, and biochemistry. There were seven bacteria that were isolated from the gut of O. rhinoceros, which were differentiated by their morphotype. The seven bacterial isolates also showed variations in physiological, and biochemical characteristics, and the larval guts were dominated by Gram-positive bacteria. The Shannon diversity index was 1.33, evenness was 0.682, richness was 7, and the abundance of each type of bacteria varied between 2x105 and 9.1x1010. Bacteria UBOG1, UBOG3, and UBOG5 were able to produce protease, but all seven bacteria were unable to produce lipase. Thus, bacterial isolates UBOG1, UBOG3, and UBOG5 are potential to be used in industry.

Bark and ambrosia beetles are commonly found in forest ecosystems, and their fungal symbionts act as plant pathogens. Accurate information on the presence and population levels of bark and ambrosia beetles is becoming an increasingly crucial to protect the forest ecosystem. This study compared multiple-funnel and bottle traps to monitor the bark and ambrosia beetles in two types of mahogany forest. Multiple-funnel and bottle traps were made from transparent polyethylene terephthalate bottles and baited with 95% ethanol. Two different mahogany forest management (monoculture and polyculture with coffee plants) were used in this study. A total of 2,367 bark and ambrosia beetle specimens were trapped in this study. This research recorded ten genera of Scolytinae and one Platypodinae genus. Xyleborus spp., X. crassiusculus, and P. cavipennis are three predominated ambrosia beetles collected in mahogany monoculture and polyculture. Euplatypus parallelus was identified as a representative of Platypodinae. Results showed that multiple-funnel traps baited with ethanol are effective to catch individuals and several species of bark and ambrosia beetles in mahogany forests. The choice of multiple-funnel or bottle traps is an important consideration in trap-based programs for monitoring or detecting invasive species in forest ecosystems.

This study aims to develop an ethnoscience-based organic chemistry practicum e-Module, determine the feasibility of the developed e-Module, and test its effectiveness in improving the problem-solving abilities of Chemistry Education students. The development procedure in this research is adapted from Thiagarajan (4D), which consists of Define, Design, Develop, and Disseminate. The e-Module trial involved Chemistry Education students at Lambung Mangkurat University, consisting of five people for individual trials, 10 for small group trials, and 19 for field trials. Data was collected using response questionnaires and learning achievement tests. The feasibility test shows that the e-Module is in the very good category based on the assessment of media experts (88%), material experts (90%), and Education experts (89%). Student responses to using e-Modules were also very positive. There is a significant difference between the student's pretest and post-test after using the e-Module with sig

Abstract Background Resectable pancreatic cancer (RPC) is potentially resectable on admission, and the impact of neoadjuvant therapy on these tumors is controversial. Moreover, the safety and efficacy of neoadjuvant chemoradiotherapy with moderately hypofractionated intensity‐modulated radiation therapy (NACIMRT) for RPC have not been studied. Here, we conducted a phase II study to evaluate the safety and efficacy of hypofractionated NACIMRT for RPC. Methods A total of 54 RPC patients were enrolled and treated according to the study protocol. We used moderately hypofractionated (45 Gy in 15 fractions) IMRT with gemcitabine to shorten the duration of radiotherapy and reduce gastrointestinal toxicity. The primary endpoint was overall survival (OS), and we subsequently analyzed the microscopically margin‐negative resection (R0) rate, disease‐free survival (DFS), and histologic effects and safety of NACIMRT. Results Median OS for the cohort was 40.0 months. Forty‐two patients (77.8%) underwent pancreatectomy after NACIMRT. Median DFS was 20.3 months. The R0 resection rate was 95.2% (40/42) per protocol and 85.2% (46/54) for the cohort. There were no intervention‐related deaths during the study period. Local treatment response, as assessed by the CAP classification, showed no residual tumor in 4.8% of patients. Overall, 23.9% of patients experienced CTCAE grade 3 or 4 during NACIMRT. Adjuvant therapy was initiated in 88% of patients undergoing resection. Postoperative complications grade ≥3b on the Clavien–Dindo scale occurred in 4.8% of patients. CA19‐9 level at enrollment was an independent prognostic factor for OS and DFS. Conclusions This is the first prospective study of hypofractionated IMRT as neoadjuvant therapy for RPC. Hypofractionated NACIMRT for RPC could be safely introduced with a high induction rate of adjuvant chemotherapy, with an overall survival of 40.0 months.

Abstract Background The Cockcroft–Gault formula is commonly used as a substitute for glomerular filtration rate (GFR) in Calvert's formula for carboplatin dosing, where adjusting serum creatinine measured using the enzymatic method with 0.2 mg/dL has been suggested in Japan. However, the effects of these adjustments on efficacy in patients with non‐small‐cell lung cancer remain unknown. Methods We conducted a post hoc analysis of the PREDICT1 study (CJLSG1201), a multicenter prospective observational trial of carboplatin–pemetrexed. Glomerular filtration rate values in Calvert's formula were back‐calculated from the administered dosages of carboplatin and the reported value of the target area under the curve. We estimated the serum creatinine adjustments and divided the patients into crude and adjusted groups. Results Patients in the crude group (N = 169) demonstrated similar efficacy to those in the adjusted group (N = 104) in progression‐free survival (PFS) and overall survival (OS) (hazard ratio [HR], 1.02; 95% confidence interval [CI], 0.76–1.35; p = 0.916 vs. HR, 0.87; 95% CI, 0.65–1.17; p = 0.363), with higher grade 3–4 hematologic toxicity. Among patients aged ≥75 years, the crude group (N = 47) showed superior efficacy compared with the adjusted group (N = 17) in PFS and OS (HR, 0.37; 95% CI, 0.20–0.69; p = 0.002 vs. HR, 0.43; 95% CI, 0.23–0.82; p = 0.010). Conclusions Serum creatinine adjustment may be associated with similar efficacy compared to the crude serum creatinine value. In older patients, the adjustment should be cautiously applied owing to the potential for reduced efficacy.

Background ＆ aims: Optimizing treatments balancing prognosis and therapeutic invasiveness is important in the management of pancreatic cancer (PC) owing to global ageing. This study aimed to verify the different utility of biomarkers by patients’ age. Materials ＆ methods: This is a single-center, retrospective cohort analysis involving 160 patients who undertook pancreaticoduodenectomy (PD) for PC. After comparing clinicopathological factors and survival after PD between aged (≥70 y/o) and young (＜70 y/o) patients, we compared neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), controlling nutrition (CONUT) score, carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19–9 as well as clinicopathological factors between long and short survivors in each group. We also performed Kaplan-Meyer analysis between patients stratified by biomarkers. Results: Overall survival (OS) was significantly worse in aged patients (p = 0.002). In aged patients, CEA was significantly higher in short survivors. In young patients, CONUT score and CA19-9 were higher in short survivors. Kaplan-Meyer analysis showed that NLR and CEA stratified OS in aged patients, whereas CONUT score and CA19-9 could stratify OS in young patients. Conclusion: Our current results suggest that these biomarkers had different impact on survivals according to the patients’ age.

To discuss and consider the necessary conditions for magnetic-wheeled robots with planetary-geared magnetic wheels, this paper provides comparing static calculations about three orientations in running a flange with real experiments. SCPREM-I, a magnetic-wheeled robot, was developed for running through a flange from the bottom to the top. This robot has four magnetic wheels with a built-in planetary gearset. In experiments, however, the robot sometimes fails to run through a flange in three orientations. In this study, we statically analyze SCPREM-I to find the conditions necessary for running through the flange. We calculate the forces around the front and rear wheels in the three orientations. As a result, it has been found that the chassis of the SCPREM-I applies a forward force to the wheels when it runs through the flange. In addition, it has been found that the normal force of the A-Legs is balancing with the driving force of the wheels when the SCPREM-I fails to run through the flange.

Oryctes rhinoceros merupakan serangga fitofag dan telah dilaporkan sebagai hama di beberapa negara. Larva O. rinocheros makan pada bahan organik (bagian tanaman yang membusuk) dan tidak menyebabkan kerusakan ekonomi. Seperti pada kebanyakan serangga, O. rhinoceros berinteraksi dengan mikroorganisme pada sistem pencernaanya. Selain itu, mikroorganisme dalam saluran pencernaan serangga juga merupakan sumber daya alam yang bermanfaat untuk kepentingan manusia, seperti enzim-enzim yang memiliki arti penting dalam bidang industri dan digunakan sebagai agens bioremediator untuk mengatasi masalah pencemaran lingkungan. Penelitian ini bertujuan untuk mengeksplorasi dan mempelajari keanekaragaman khamir dalam saluran pencernaan larva O. rhinoceros. Pada studi ini, khamir diisolasi dari saluran pencernaan instar tiga O. rhinoceros dan ditumbuhkan pada media Yeast Malt Agar (YMA). Berdasarkan ciri makroskopis dan mikroskopis, 7 isolat khamir yang diperoleh teridentifikasi sebagai Candida sp. (isolat K1 dan K4), Pichia sp. (isolat K3), dan Debaromyces sp.(K2, K5, K6 dan K7).

Penggunaan teknologi dalam Pendidikan menjadi sebuah kebutuhan yang tak terhindarkan pada era digital ini. Artikel ini membahas pelaksanaan pelatihan bagi dosen FKIP Universitas Cenderawasih (UNCEN) dalam penerapan Learning Management System (LMS). Pelatihan ini bertujuan untuk meningkatkan pemahaman dan keterampilan dosen dalam memanfaatkan LMS sebagai sarana untuk mengelola materi pembelajaran, menyajikan materi secara interaktif, serta memfasilitasi komunikasi antara dosen dan mahasiswa. Metode pelatihan mencakup sesi pelatihan praktis, studi kasus, dan diskusi kelompok. Evaluasi hasil pelatihan menunjukkan peningkatan yang signifikan dalam pemahaman dan keterampilan dosen terkait pemanfaatan LMS. Para peserta pelatihan menunjukkan kemajuan dalam mengelola kursus, memanfaatkan fitur interaktif LMS, dan meningkatkan komunikasi dengan mahasiswa. Pelatihan ini tidak hanya berhasil dalam mencapai tujuannya untuk meningkatkan kompetensi dosen terkait LMS, tetapi juga memberikan kontribusi positif terhadap kualitas pengajaran dan interaksi antara dosen dan mahasiswa. Kesimpulannya, integrasi LMS melalui pelatihan ini dapat dianggap sebagai Langkah strategis dalam memenuhi tuntutan Pendidikan di era digital.

Abstract Various liquid biopsy methods have been developed for the non-invasive and early detection of diseases. In particular, the detection of circulating tumor cells (CTCs) and cancer-associated fibroblasts (CAFs) in blood has been receiving a great deal of attention. We have been developing systems and materials to facilitate such liquid biopsies. In this study, we further developed glass filters (with various patterns of holes, pitches, and non-adhesive coating) that can capture CTCs, but not white blood cells. We optimized the glass filters to capture CTCs, and demonstrated that they could be used to detect CTCs from lung cancer patients. We also used the optimized glass filters for detecting CAFs. Additionally, we further developed a system for visualizing the captured cells on the glass filters. Finally, we demonstrated that we could directly culture the captured cells on the glass filters. Based on these results, our high-performance glass filters appear to be useful for capturing and culturing CTCs and CAFs for further examinations.

This study aimed to examine minimodeling-based bone formation between the epiphyses and metaphyses of the long bones of eldecalcitol (ELD)-administered ovariectomized rats. Sixteen-week-old female rats were divided into four groups: sham-operated rats receiving vehicle (Sham group), ovariectomized (OVX) rats receiving vehicle (Vehicle group), or ELDs (30 or 90 ng/kg BW, respectively; ELD30 and ELD90 groups). ELD administration increased bone volume and trabecular thickness, reducing the number of osteoclasts in both the epiphyses and metaphyses of OVX rats. The Sham and Vehicle groups exhibited mainly remodeling-based bone formation in both regions. The epiphyses of the ELD groups showed a significantly higher frequency of minimodeling-based bone formation than remodeling-based bone formation. In contrast, the metaphyses exhibited significantly more minimodeling-based bone formation in the ELD90 group compared with the ELD30 group. However, there was no significant difference between minimodeling-based bone formation and remodeling-based bone formation in the ELD90 group. While the minimodeling-induced new bone contained few sclerostin-immunoreactive osteocytes, the underlying pre-existing bone harbored many. The percentage of sclerostin-positive osteocytes was significantly reduced in the minimodeling-induced bone in the epiphyses but not in the metaphyses of the ELD groups. Thus, it seems likely that ELD could induce minimodeling-based bone formation in the epiphyses rather than in the metaphyses, and that ELD-driven minimodeling may be associated with the inhibition of sclerostin synthesis.

The advance of laser-driven neutron sources (LDNSs) has enabled neutron resonance spectroscopy to be performed with a single shot of a laser. In this study, we describe a detection system of epithermal (∼eV) neutrons especially designed for neutron resonance spectroscopy. A time-gated photomultiplier tube (PMT) with a high cut-off ratio was introduced for epithermal neutron detection in a high-power laser experiment at the Institute of Laser Engineering, Osaka University. We successfully reduced the PMT response to the intense hard X-ray generated as a result of the interaction between laser light and the target material. A time-gated circuit was designed to turn off the response of the PMT during the laser pulse and resume recording the signal when neutrons arrive. The time-gated PMT was coupled with a 6Li glass scintillator, serving as a time-of-flight (TOF) detector to measure the neutron resonance absorption values of 182W and 109Ag in a laser-driven epithermal neutron generation experiment. The neutron resonance peaks at 4.15 eV of 182W and 5.19 eV of 109Ag were detected after a single pulse of laser at a distance of 1.07 m.

The problem in this study is the low activity and student learning outcomes in learning natural sciences. The INTERNAL model integrates Inquiry, Number Head Together, and snowball throwing. This study uses a qualitative approach and the type of classroom action research (CAR), which was held for four meetings. Data analysis in the form of teacher activity sheets, student activity sheets, and student learning outcomes sheets. Data collection techniques in the form of observation and writing tests. The study subjects were the fifth-grade students of SDN Swato 2 Tapin for the academic year 2021/2022, a total of 32 students. The activity success indicator scores 58-72, and student learning outcomes with minimum standards 80%. The study results indicate that the teacher's activity at the first meeting scored 54 with the "Good" criteria, increasing at the fourth meeting with a score of 74 with the "Verry Good" criteria. Student activity classically at the first meeting reached 25" on the "Very Active" criteria, increased at the fourth meeting to 83,3% with the "Very Activity" criteria. Student learning outcomes at the first meeting with 50% classical completeness increased at the fourth meeting with 100% classical completeness. It can be concluded that applying the internal learning model can improve student activities and learning outcomes.

This study aims to analyze the causal relationship between the exchange rate and export value in Indonesia using time series data from 1997 to 2020. The analytical method that will be used in this research is the Granger Causality Test approach. The instruments used are the data normality test, the stationary test, the optimal lag test, and the Granger causality test. Based on the results of the research that has been done, it shows that from the output of the cointegration test in table 4.3 above, the trace statistic is 9.016078, which is smaller than the critical value (5%), which is 15.49471, and Prob. 0.3639 is less than 5 percent, whereas for the output of the Granger causality test above, at lag 1, it is known that the probability value (Prob.) of the relationship between export value and exchange rate is 0.4953; this result is greater than the significance level of 5 percent, so that from the output of the From these results, it can be concluded that there is no causal relationship between export values and exchange rates. So it can be concluded that if the exchange rate depreciates against foreign currencies, the export value will increase because Indonesia's export commodities compete in international markets, but on the contrary, it will reduce. In addition, based on observations and causality analysis from the results of data processing, it shows that there has been a one-way relationship between the exchange rate and the export value, meaning that the exchange rate will affect the export value but will, on the contrary, affect the export value. According to the results of the Granger Causality Test, the export value has no effect on the exchange rate.