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Additional file 1: Table S1. Table of the number of mice used during the experiment. Figure S1. Original image of protein immune bands. (A) Original images of western blot of protein IL-1β. (B) Original images of western blot of protein IL-18. (C) Original images of western blot of protein cleaved-caslase-1. (D) Original images of western blot of protein PSD95. (E) Original images of western blot of protein MAP2. (F) Original images of western blot of protein α-Tubulin. (G) Original images of western blot of protein NLRP3. Figure S2. The results of the Morris water maze experiment in two groups of mice in the pre-experiment. (A) Representative water maze trajectories of mice in Sham and TF groups. (B) The number of water Morris maze cross-platform for two groups of mice. (C) The total distance of Morris water maze in two groups of mice. (D) Morris water maze escape latency of two groups of mice. (E) The time spent in the target quadrant of two groups of mice. Data are presented as the mean ± SD (n = 10 mice/group). Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test or Kruskal–Wallis and Dunn’s multiple comparison test. Figure S3. Weight gain curves of WT and NLRP3-cKO mice. Figure S4. Astrocytes-specific NLRP3 knockout in combination with selegiline administration did not significantly improve impulse-like behaviors and cognitive dysfunction after TF surgery. (A) Computer printouts showing the shifting trajectories of each group in the OFT at 8 days after surgical exposure. (B) The total distance for each group at 8 days after surgical exposure. (C) Computer printouts showing the shifting trajectories of each group in the EPM at 8 days after surgical exposure. (D) The time spent in the open arm for each group at 8 days after surgical exposure. (E) Freezing time during the FC test results caused by the indicated stimuli. Data are presented as the mean ± SD (n = 12 mice/group). Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test or Kruskal–Wallis and Dunn’s multiple comparison test. ****P < 0.0001; *P < 0.05. Figure S5. Changes in excitatory and inhibitory neurons in the CA3 region of the hippocampus in each group. (A) Image of CA1 in hippocampus. (B) Representative photomicrographs of VGLUT2 and GAD65 in the CA3 of the hippocampus. Scale bar = 25 μm. (C, D) The intensity of VGLUT2 and GAD65 was quantified in each group. (E) Representative photomicrographs of VGLUT2 and GAD65 in the CA3 of the hippocampus. Scale bar = 25 μm. (F, G) The intensity of VGLUT2 and GAD65 was quantified in each group. (H) Representative photomicrographs of VGLUT2 and GAD65 in the CA3 of the hippocampus. Scale bar = 25 μm. (I, J) The intensity of VGLUT2 and GAD65 was quantified in each group. Data are presented as the mean ± SD (n = 6 mice/group). Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test or Kruskal–Wallis and Dunn’s multiple comparison test. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05.

A high-performance capillary electrophoresis with amperometry detection method (CE-AD) has been developed for the analysis of flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and rhein) in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17 min using a borate–phosphate running buffer (1.5 × 10−2 mol/L borate–3 × 10−2 mol/L phosphate running buffer, pH 9.0) at a working potential of +0.90 V (vs. SCE) and a separation voltage of 19 kV. The linear relationship between concentration and current response was obtained with detection limits (S/N = 3) ranging from 1.0 × 10−7 to 2.1 × 10−7 g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.

A sensitive flow injection chemiluminescence method has been developed for the detection of resveratrol in red wine based on the fact that resveratrol can greatly enhance chemiluminescence reaction between KMnO 4 and HCHO in sulfuric acid medium. Analytes were pre-concentrated on solid sorbents (C 18 solid-phase extraction cartridges). Under the optimum conditions, the proposed method allows the measurement of resveratrol over the range of 1.32 × 10 −8 to 1.32 × 10 −5 mol/L with a detection limit of 3.30 × 10 −9 mol/L, and the relative standard deviation for 1.32 × 10 −5 mol/L resveratrol ( n = 11) is 3.8%. This method has been successfully applied for the determination of the resveratrol in red wine. Furthermore, the possible reaction mechanism was also discussed.

A method based on capillary electrophoresis coupled with amperometric detection (CE-AD) has been developed for the separation and determination of kaempferol, ferulic acid, vanillic acid, caffeic acid, gallic acid and protocatechuic acid in blueberry wine for the first time. In order to get the optimum conditions of separation, the effects of working electrode potential, pH value and concentration of running buffer, separation voltage and injection time on CE-AD were investigated. Under the optimum conditions, the analytes could be separated in Na 2 B 4 O 7 –KH 2 PO 4 buffer at pH 7.8 within 18 min. A 300 μm diameter carbon disk electrode had good current responses at +0.95 V ( vs . SCE) for all analytes. The responses were linear with concentrations over three orders of magnitude with detection limits (S/N = 3) at 10 −8 g/mL magnitude for the analytes and the recoveries were in the range of 95.8–106.7%. The method could be successfully applied to the analysis of real sample with satisfactory results and therefore recommended for use by the quality control departments of fruit wine producers.