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Abstract Point cloud completion technology is used to address incomplete three-dimensional point cloud data, predicting and reconstructing the original shape and details to achieve virtual restoration. While existing learning-based methods have made significant progress in point cloud completion, they still face challenges when dealing with noise and invisible data. To address these issues, this paper proposes a multi-layer upsampling network based on a graph attention mechanism, called GANet. GANet consists of three main components: (1) feature extraction; (2) seed point generation; (3) State Space Model-based Point Cloud Upsampling Layer. GANet demonstrates exceptional robustness in handling noise and invisible data. To validate the effectiveness of GANet, we applied it to Terracotta Warrior data. The Terracotta Warriors, as important cultural heritage, present a challenging test case due to damage and missing parts caused by prolonged burial and environmental factors. We trained and tested GANet on both the PCN dataset and Terracotta Warrior data, comparing it with several recent learning-based methods. Experimental results show that GANet can effectively reconstruct missing or damaged parts of 3D point clouds, providing more detailed and structurally accurate completion results. These completion models not only validate GANet’s effectiveness but also offer valuable references for cultural heritage restoration work.

BackgroundInjury is a major public health issue, and studying the correlation between temperature and injury in the context of climate change is of great significance for injury prevention and control.ObjectiveTo analyze the association between temperature and the volume of emergency medical service response due to injury in Chengdu. MethodsUsing a retrospective ecological study design, weather data in Chengdu were collected from 1 January 2022 to 31 December 2023 from Chengdu Meteorological Office, including daily average temperature, daily average relative humidity, average wind speed, average pressure, sunshine, and rainfall. Emergency medical service data were collected from an emergency medical service center in Chengdu. A distributional lag non-linear model was conducted to calculate the association between temperature and volume of emergency medical service response due to injury, and gender- and age-stratified analyses were followed to identify susceptible groups. ResultsOverall 219685 incidents of emergency medical service dispatching due to injury were collected during the study period. The top three injury causes were traffic accident (90765, 41.32%), poisoning (44101, 20.07%), and falling (44046, 20.05%). When daily average temperature exceeded 17.9 ℃, as the temperature rised, the volume of emergency medical service response for injury increased. In terms of the single-day lag effect, the risk of emergency medical service response for injury elevated on lag1 and persisted until lag6, the maximum daily effectwas observed on lag2, lag3 and lag4, and their relative risks associated with every 1℃ higher above 17.9 ℃ were both 1.08 times, 95%CIs were (1.03, 1.14), (1.04, 1.12), and (1.04, 1.16) respectively. Both men and women were susceptible to temperature, but on the same lag day, the men's RR value was higher than the women's. There was no statistical difference in the lag effect of temperature on the 0-17 age group; the lag effect on the 18-44 age group appeared on lag1, and lasted to lag6; that for the 45-64 age group appeared on lag1, and lasted to lag4; that for the 65 age group and over was only on lag 3 (RR: 1.05, 95%CI: 1.00, 1.10). ConclusionAs the temperature rises, the volume of emergency medical service response due to injury is increased in Chengdu. In particular, the associated impact is significant on males and people aged 18-44 years old.

Abstract Background This study aimed to identify the differentially expressed proteins in the vitreous humor (VH) of eyes with and without pathologic myopia (PM), providing insights into the molecular pathogenesis. Methods A cross-sectional, observational study was conducted. VH samples were collected from patients undergoing vitrectomy for idiopathic epiretinal membrane (ERM), macular hole (MH), or myopic retinoschisis (MRS). Label-free quantitative proteomic analysis identified differential protein expression, with validation using ELISA. Results The proteomic profiling revealed significantly higher expressions of tubulin alpha 1a (TUBA1A) and eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) in PM groups (MH-PM, MRS-PM) compared to controls (MH, ERM). Conversely, xylosyltransferase 1 (XYLT1), versican core protein (VCAN), and testican-2 (SPOCK2) expressions were lower in PM. ELISA validation confirmed these findings. Conclusions Our study provides novel insights into the molecular mechanisms of PM. The differentially expressed proteins EEF1A1, TUBA1A, XYLT1, VCAN, and SPOCK2 may play crucial roles in chorioretinal cell apoptosis, scleral extracellular matrix (ECM) synthesis, and scleral remodeling in PM. These proteins represent potential new targets for therapeutic intervention in PM, highlighting the importance of further investigations to elucidate their functions and underlying mechanisms in disease pathogenesis.

The urgent need to reduce agricultural water consumption and tackle water scarcity has made developing water-saving technologies in agriculture a top priority. However, However, introducing these technologies does not automatically guarantee a reduction in overall water consumption within the sector. The rebound effect plays a significant role in limiting the effectiveness of water conservation policies. A comprehensive understanding of irrigation's water rebound effect (WRE) is essential for successfully conserving agricultural water resources, especially in arid regions. This study focuses on Xinjiang and empirically analyzes the rebound effect following the implementation of water-saving measures. This study focuses on Xinjiang and empirically analyzes the rebound effect following the implementation of water-saving measures. The findings indicate that from 2001 to 2020, water consumption remained a concern despite advancements in irrigation technology, leading to a 40.86 % reduction in the irrigation quota (a decrease of 5567.12 m³/ha). The cultivated land area, irrigation area, water-saving irrigation area, agricultural output value, agricultural water consumption, and agricultural water use productivity in Xinjiang have increased by 2.86 × 106 ha, 1.40 × 106 ha, 1.70 × 106 ha, 81.61 × 109 CNY, 6.28 × 109 m3 and 1.57 CNY/m3, respectively, with growth rates of 83.54 %, 41.27 %, 134.24 %, 217.87, 7.80 % and 192.83 %, respectively. Despite the reduced water allocation per hectare for irrigation, the anticipated decline in total water consumption did not occur, revealing a significant rebound effect, with variations ranging from 64.84 % to 1972.51 %. This indicates that a single water-saving measure may not ensure long-term water conservation. A deeper analysis of this rebound effect can help formulate more effective water management strategies. Strategies should encompass promoting ongoing advancements in water-saving technologies, imposing restrictions on expanding the cultivated land in certain areas, decreasing the cultivation of high-yield and high-water-demand crops, curbing the extension of irrigation coverage, enhancing the establishment of agricultural water pricing mechanisms, integrating smart irrigation technologies and data-driven water resource management, enhancing the utilization of saline-alkaline water, and driving policy innovation. The findings can help enhance agricultural water use efficiency, supporting agricultural production and regional economic development. This not only aids in improving agricultural water management in Xinjiang but also provides valuable insights for other arid and semi-arid regions. These insights can aid in creating more efficient water resource management strategies, reducing the negative impacts of the WRE, and advancing global agricultural sustainability and innovation in water management.

IntroductionTo establish a high myopia model in C57BL/6J mice with monocular form deprivation myopia (FDM) and investigate its ocular structure pathological trajectory.MethodsHealthy 3-week-old C57BL/6J mice were divided into an FDM group (n = 36) and a control group (n = 24). The left eyes of the FDM group were patched, while the right eyes served as controls. Biometric parameters and fundus morphology were assessed at baseline and after 4, 8, and 12 weeks of form deprivation.ResultsSignificant differences were observed in the deprived eyes, including longer axial length, higher refractive power, deeper vitreous chambers, thinner retina, choroid, and sclera, and smaller scleral fibers’ diameters under a transmission electron microscope. Retinal vascular area proportion in covered eyes decreased significantly (P < 0.05), with a decline rate of 11% from weeks 4 to 8 and a faster decline of 19% from weeks 8 to 12, while this proportion increased significantly in control eyes.DiscussionThis study successfully induced a high myopia model in mice with long-term form deprivation. The axial length grew dramatically in FDM in the first 8 weeks, while the pathological progress of the fundus accelerated from weeks 8 to 12.

IntroductionPhytohormones play a crucial role in regulating the abscission of plant organs and tissues.MethodsIn this study, the ultrastructure of the sepals of Korla fragrant pears was observed using a transmission electron microscope, and high-performance liquid and gas chromatography were used to analyze the dynamic changes of phytohormones in the abscission zone during the calyx abscission process of Korla fragrant pears, and mass spectrometry imaging was applied to ascertain the spatial distribution of phytohormones.ResultsThe results revealed that the mitochondria in the abscission zone of the decalyx fruits were regularly distributed around the cell wall, and the chloroplasts were moderately present. In contrast, in the persistent calyx fruit, the corresponding parts of the abscission zone showed a scattered distribution of mitochondria within the cells, and there was a higher number of chloroplasts, which also contained starch granules inside. Mass spectrometry imaging revealed that ABA was enriched in the abscission zone of the decalyx fruit, and their ionic signal intensities were significantly stronger than those of the persistent calyx fruit. However, the ionic signal intensities of Indole-3-acetic acid (IAA) and Gibberellin A3 (GA3) of the persistent calyx fruit were significantly stronger than those in the abscission zone of the decalyx fruit and were concentrated in the persistent calyx fruit. 1-Aminocyclopropanecarboxylic Acid (ACC) did not show distinct regional distribution in both the decalyx and persistent calyx fruits. Furthermore, before the formation of the abscission zone, the levels of IAA, GA3, and zeatin (ZT) in the abscission zone of the decalyx fruits were significantly lower than those in the persistent calyx fruits by 37.9%, 57.7%, and 33.0%, respectively, while the levels of abscisic acid (ABA) and ethylene (ETH) were significantly higher by 21.9% and 25.0%, respectively. During the formation of the abscission zone, the levels of IAA, GA3, and ZT in the abscission zone of the decalyx fruits were significantly lower than those in the persistent calyx fruits by 41.7%, 71.7%, and 24.6%, respectively, while the levels of ABA and ETH were significantly higher by 15.2% and 80.0%, respectively. After the formation of the abscission zone, the levels of IAA and GA3 in the abscission zone of the decalyx fruits were lower than those in the persistent calyx fruits by 20.8% and 47.8%, respectively, while the levels of ABA and ETH were higher by 271.8% and 26.9%, respectively. In summary, during the calyx abscission process of Korla fragrant pears, IAA and GA3 in the abscission zone inhibited abscission, while ABA and ETH promoted calyx abscission. These research findings enrich the understanding of the regulatory mechanism of plant hormones on calyx abscission and provide a theoretical basis for the study of exogenous plant growth regulators for regulating calyx abscission in Korla fragrant pear.

In arid areas, biodegradable film has recently had the potential to replace polyethylene (PE) film to address plastic pollution. However, the positive effect of biodegradable film on soil moisture and salt control is weaker than that of PE film. Magnetized irrigation water technology is expected to compensate for this limitation. This study comprised a field experiment in 20212023 to study how two types of biodegradable film (M1, black; M2, transparent) and six magnetization intensity on irrigation water (T0, 0 Gs; T1, 1000 Gs; T2, 2000 Gs; T3, 3000 Gs; T4, 4000 Gs; T5, 5000 Gs) affect the degradation rate of biodegradable film, soil watersalt distribution, growth, and quality of processing tomatoes. The traditional PE film mulching and non-magnetized irrigation were used as the control group (MPET0). The results demonstrated that magnetized water irrigation slowed down the degradation rate of biodegradable film. In addition, the magnetized irrigation water can redistributed the soil water-salt patterns under the biodegradable film, improving the soil water content and salt leaching efficiency, with better results in M1 than in M2. Moreover, magnetized water irrigation promoted the growth of tomato leaf area under the biodegradable film, enhancing photochemical efficiency and potential activity of PSII, thereby improving fruit yield, quality, and water use efficiency of tomato. Principal component analysis showed that the comprehensive score of M1T3 treatment was the highest throughout the three years. Furthermore, M1T3 treatment has the highest processing tomato economic benefits during 2021–2023 (24634986 CNY hm2 more than MPET0). Therefore, the use of 3000 Gs magnetized irrigation water combined with black biodegradable film is conducive to improving soil water and salt conditions, reducing residual film pollution, and improving the yield and quality of processing tomatoes, thus ensuring the sustainable development of oasis agriculture.

Resveratrol, a naturally occurring polyphenolic compound, has captivated the scientific community with its promising therapeutic potential across a spectrum of diseases. This review explores the complex role of resveratrol in modulating ferroptosis, a newly identified form of programmed cell death, and its potential implications for managing cardiovascular and cerebrovascular disorders, cancer, and other conditions. Ferroptosis is intricately linked to the pathogenesis of diverse diseases, with resveratrol exerting multifaceted effects on this process. It mitigates ferroptosis by modulating lipid peroxidation, iron accumulation, and engaging with specific cellular receptors, thereby manifesting profound therapeutic benefits in cardiovascular and cerebrovascular conditions, as well as oncological settings. Moreover, resveratrol’s capacity to either suppress or induce ferroptosis through the modulation of signaling pathways, including Sirt1 and Nrf2, unveils novel therapeutic avenues. Despite resveratrol’s limited bioavailability, advancements in molecular modification and drug delivery optimization have amplified its clinical utility. Future investigations are poised to unravel the comprehensive mechanisms underpinning resveratrol’s action and expand its therapeutic repertoire. We hope this review could furnish a detailed and novel insight into the exploration of resveratrol in the regulation of ferroptosis and its therapeutic prospects.

The development of domestic oilfield is directly related to the national energy security. The domestic oilfield development and production resulted in the generation of large amount of wastewater. The efficient treatment and resource utilization of oilfield wastewater is the key to the green and low-carbon development of China’s petroleum industry. At present，coagulation has become an indispensable technology during oilfield wastewater treatment because of its maturity，economic efficiency and practicability. In this paper，the status and progress of coagulation process in oilfield wastewater treatment were reviewed. The commonly used coagulation process in oilfield wastewater treatment were emphatically introduced. The typical coagulants used in oilfield wastewater treatment were also illustrated. Furthermore，the removal performances and characteristics of novel composite coagulants were specified. On this basis，the processes for enhancing the coagulation performance were introduced. In addition，combining with the practical engineering application，the problems within the coagulation process and the development direction were evaluated emphatically. It was hoped to provide references for the research，development and application of coagulation in oilfield wastewater treatment.

AimsThe health management of end-stage renal disease patients is a complicated process, and mobile health management technology provides a new choice for the health management of end-stage renal disease patients. The scope of clinical studies on mobile health management for patients with end-stage renal disease was reviewed, and found that about mobile health management problems existing in the literature were identified to provide ideas for subsequent mobile health management research.MethodsThe databases Web of Science, PubMed, The Cochrane Library, Embase, CNKI, Wan Fang Data, BMJ, and VIP were systematically searched for studies on Mobile health management among end-stage renal disease in adult and adolescent patients or children undergoing kidney replacement therapy. The search covered the period from the inception of the databases to June 20, 2023. Two independent reviewers conducted the literature screening process. Following eligibility screening, a total of 38 papers were included for data extraction and descriptive analysis.ResultsA total of 38 studies from 14 countries were finally included. The majority of which were interventional trials. The platforms used in these studies included remote monitoring systems, apps, websites, mobile phones or tablets, and social platforms. These platforms provided patients with a wide range of services, including disease management, behavioral intervention, social support, and follow-up care. Most studies focused on patient clinical indicators, patient experience, quality of life, and healthcare costs.ConclusionOur findings that mobile health management has been widely used in disease management of end-stage renal disease patients, with rich management content and many evaluation indicators. Future studies should strengthen the evaluation of patients’ mental health, quality of life, and healthcare costs. Additionally, developing a clinical decision support system would enable mobile health management to play a more effective role in end-stage renal disease patients.

The discovery of MPTP, an industrial chemical and contaminant of illicit narcotics, which causes parkinsonism in humans, non-human primates and rodents, has led to environmental pollutants exposure being convicted as key candidate in Parkinson’s disease (PD) pathogenesis. Though MPTP-induced mitochondrial dysfunction and neuroinflammation are mainly responsible for the causative issue of MPTP neurotoxicity, the underlying mechanism involved remains unclear. Here, we reveal a novel signaling mechanism of CDK5-USP30-MAVS regulating MPTP/MPP+ induced PD. MPP+ (the toxic metabolite of MPTP) treatment not only led to the increased protein levels of USP30 but also to mitophagy inhibition, mitochondrial dysfunction, and MAVS-mediated inflammation in BV2 microglial cells. Both mitophagy stimulation (Urolithin A administration) and USP30 knockdown relieved MAVS-mediated inflammation via restoring mitophagy and mitochondrial function in MPP+-induced cell model. Notably, MPTP/MPP+-induced CDK5 activation regulated USP30 phosphorylation at serine 216 to stabilize USP30. Moreover, CDK5-USP30 pathway promoted MAVS-mediated inflammation in MPTP/MPP+-induced PD model. Inhibition of CDK5 not only had a protective effect on MPP+-induced cell model of PD via suppressing the upregulation of USP30 and the activation of MAVS inflammation pathway in vitro, but also prevented neurodegeneration in vivo and alleviated movement impairment in MPTP mouse model of PD. Overall, our study reveal that CDK5 blocks mitophagy through phosphorylating USP30 and activates MAVS inflammation pathway in MPTP/MPP+-induced PD model, which suggests that CDK5-USP30-MAVS signaling pathway represents a valuable treatment strategy for PD induced by environmental neurotoxic pollutants in relation to MPTP.

Biodegradable film (BF), as a superior substitute for traditional polyethylene film (PF), has experienced a consistent increase in China. However, a significant research gap persists regarding the utilization of BF in assessing farmland carbon balance. This experiment examined the responses of maize growth, yield, irrigation water productivity (IWP), CO2 emissions, and field net carbon sequestration (NCS) to two BF types (BF1, 100-day induction period, and BF2, 80-day induction period), and PF, and three irrigation amounts (I1, I2, I3: 5250, 5625, and 6000 m3 ha–1), by conducting a two-year (2019–2020) field trial in a typical arid and semi-arid region of China. The results indicated that biodegradable film mulching (BFM) negatively affected maize growth, resulting in reduced yield and IWP. Especially under the I1 irrigation level, the biodegradable film reduced maize yield by 6.26%–13.99% and IWP by 6.64–13.85%. Notably, in the I2 irrigation level, BF1 mulching had a comparable impact on maize yield and IWP to that of PF. Additionally, the increase in irrigation amounts exerted a more pronounced promoting effect on maize total biomass than on yield and IWP. Concerning carbon balance, short-term BF application resulted in reduced CO2 emissions (5.74–6.30% under BF1 and 9.27–12.37% under BF2), without any significant impact on NCS in cropland. Taking into account the economic value of maize and farmland ecology, we recommended utilizing irrigation amounts of 5625 m3 ha–1 for BF application in arid areas, while opting for a BF with a longer induction period whenever feasible.

The application of machine learning (ML) in structural engineering is receiving increasing attention recently. This paper experimentally studies three self-restoring reinforced concrete (SRRC) columns reinforced with low-bond ultra-high strength rebars, to first discuss the reliability and evaluation of the SRRC columns under multiple reversed cyclic (MRC) loads induced by strong earthquakes, and to also first introduce the Transformer method into the analysis and discussion of structural tests. The tests confirmed the superior seismic behavior and high self-centering performance of the columns and presented how MRC loads affect the seismic performance of SRRC columns in terms of the lateral load-carrying capacity and energy dissipation capacity. Superior to conventional methods, a high-accuracy Transformer-based model is proposed to evaluate the plastic hinge height (PHL) of the tested SRRC columns compared with the other three algorithms (MLP, KNN, and XGBoost). Furthermore, the Shapley Additive exPlanations (SHAP) approach is adopted to explain the insight relationship between the structural parameters and PHL of the columns.

Organizational climate has been shown to be an important factor associated with teachers’ job satisfaction. However, the internal mechanism between them is unclear. The purpose of this study was to investigate whether the relationship between kindergarten organizational climate and kindergarten teachers’ job satisfaction was affected by occupational stress and emotional labor. This study employed a questionnaire survey method to gather data from 1,091 kindergarten teachers nationwide. It conducted an analysis of the current status of kindergarten organizational climate and the job satisfaction of kindergarten teachers, elucidating the relationship between the two and the underlying mechanisms. Additionally, a chain mediation model was constructed. The findings indicated that: (1) organizational climate, kindergarten teachers’ occupational stress and emotional labor all significantly predict kindergarten teachers’ job satisfaction directly (2) organizational climate could indirectly influence kindergarten teachers’ job satisfaction through three pathways: the separate mediating effect of occupational stress and emotional labor, and the chain mediating effect on both. The research findings highlight the significance of kindergarten organizational climate, occupational stress, and emotional labor in augmenting the job satisfaction of kindergarten teachers, offering valuable insights for the improvement of kindergarten teacher job satisfaction.

ObjectiveThe pathological classification and imaging manifestation of parotid gland tumors are complex, while accurate preoperative identification plays a crucial role in clinical management and prognosis assessment. This study aims to construct and compare the performance of clinical models, traditional radiomics models, deep learning (DL) models, and deep learning radiomics (DLR) models based on ultrasound (US) images in differentiating between benign parotid gland tumors (BPGTs) and malignant parotid gland tumors (MPGTs).MethodsRetrospective analysis was conducted on 526 patients with confirmed PGTs after surgery, who were randomly divided into a training set and a testing set in the ratio of 7:3. Traditional radiomics and three DL models (DenseNet121, VGG19, ResNet50) were employed to extract handcrafted radiomics (HCR) features and DL features followed by feature fusion. Seven machine learning classifiers including logistic regression (LR), support vector machine (SVM), RandomForest, ExtraTrees, XGBoost, LightGBM and multi-layer perceptron (MLP) were combined to construct predictive models. The most optimal model was integrated with clinical and US features to develop a nomogram. Receiver operating characteristic (ROC) curve was employed for assessing performance of various models while the clinical utility was assessed by decision curve analysis (DCA).ResultsThe DLR model based on ExtraTrees demonstrated superior performance with AUC values of 0.943 (95% CI: 0.918-0.969) and 0.916 (95% CI: 0.861-0.971) for the training and testing set, respectively. The combined model DLR nomogram (DLRN) further enhanced the performance, resulting in AUC values of 0.960 (95% CI: 0.940- 0.979) and 0.934 (95% CI: 0.876-0.991) for the training and testing sets, respectively. DCA analysis indicated that DLRN provided greater clinical benefits compared to other models.ConclusionDLRN based on US images shows exceptional performance in distinguishing BPGTs and MPGTs, providing more reliable information for personalized diagnosis and treatment plans in clinical practice.

Abstract Background Many studies have reported the association between ambient temperature and mortality from cardiovascular disease (CVD). However, the health effects of humidity are still unclear, much less the combined effects of temperature and humidity. In this study, we used humidex to quantify the effect of temperature and humidity combined on CVD mortality. Methods Daily meteorological, air pollution, and CVD mortality data were collected in four cities in southwest China. We used a distributed lag non-linear model (DLNM) in the first stage to assess the exposure–response association between humidex and city-specific CVD mortality. A multivariate meta-analysis was conducted in the second stage to pool these effects at the overall level. To evaluate the mortality burden of high and low humidex, we determined the attributable fraction (AF). According to the abovementioned processes, stratified analyses were conducted based on various demographic factors. Results Humidex and the CVD exposure–response curve showed an inverted “J” shape, the minimum mortality humidex (MMH) was 31.7 (77th percentile), and the cumulative relative risk (CRR) was 2.27 (95% confidence interval [CI], 1.76–2.91). At extremely high and low humidex, CRRs were 1.19 (95% CI, 0.98–1.44) and 2.52 (95% CI, 1.88–3.38), respectively. The burden of CVD mortality attributed to non-optimal humidex was 21.59% (95% empirical CI [eCI], 18.12–24.59%), most of which was due to low humidex, with an AF of 20.16% (95% eCI, 16.72–23.23%). Conclusions Low humidex could significantly increase the risk of CVD mortality, and vulnerability to humidex differed across populations with different demographic characteristics. The elderly (> 64 years old), unmarried people, and those with a limited level of education (1–9 years) were especially susceptible to low humidex. Therefore, humidex is appropriate as a predictor in a CVD early-warning system.

The gut microbiota is closely associated with human health, and alterations in gut microbiota can influence various physiological and pathological activities in the human body. Therefore, microbiota regulation has become an important strategy in current disease treatment, albeit facing numerous challenges. Nanomaterials, owing to their excellent protective properties, drug release capabilities, targeting abilities, and good biocompatibility, have been widely developed and utilized in pharmaceuticals and dietary fields. In recent years, significant progress has been made in research on utilizing nanomaterials to assist in regulating gut microbiota for disease intervention. This review explores the latest advancements in the application of nanomaterials for microbiota regulation and offers insights into the future development of nanomaterials in modulating gut microbiota.

Programmed cell death (PCD) is common in plant growth and development, such as xylem development, organ senescence, and abscission. Calyx abscission in Korla fragrant pear contributes to fruit quality, while it was not clear whether PCD occurred during calyx abscission and which signals regulated the process. Therefore, it is imperative to clarify the process of PCD in the process of calyx abscission in Korla fragrant pear under natural conditions to enrich the mechanism of calyx abscission. The results showed that the total time of calyx abscission of Korla fragrant pear began from 6 days after pollination (DAP) to 13 DAP, and the peak of calyx abscission occurred 10 DAP. During the whole process of calyx abscission, PCD started 6 DAP. At 9 DAP, the degree of PCD deepened. At 12 DAP, the cells in the abscission zone showed asymmetry on both sides, the organelles in the distal cells of the abscission zone degraded into apoptotic fragments, and the protective layer of the normal development of cells located at the proximal end of the abscission zone region ended the PCD process. ETH concentrations in the abscission zone of the decalyx fruit were significantly higher than those of the persistent calyx fruits in each period during calyx abscission, and high levels of ethylene and hydrogen peroxide and low contents of the GA3, ZT, and hydroxyl radicals promoted calyx abscission before the formation of the abscission zone. At 3 DAP, the ethylene concentration (43.97 ppm) and H2O2 content (8.49 μmol/g) of decalyx fruit in the abscission zone were significantly higher than those of persistent calyx fruit by 67.69% and 27.86%, respectively; however, the GA3, ZT, and hydroxyl radicals showed the opposite. Overall, PCD in the abscission zone of decalyx fruits did occur during the calyx abscission of Korla fragrant pear, and ethylene and H2O2 might play major roles in initiating the PCD process during Korla fragrant pear calyx abscission.

Cold stress is an important limiting factor affecting spring tea quality. This study analyzed the effects of foliar spraying of brassinolide (BR) at concentrations of 0.05, 0.1, 0.5, and 1 mg/L on the chlorophyll index, nitrogen balance index, quality, antioxidant defense system, and secondary metabolite profiles in leaves of Camellia sinensis cv. Fuding-dabaicha grown at 4 °C for 48 h. All exogenous BR treatments significantly increased leaf nitrogen balance index, with the highest effect at 0.1 mg/L, which also significantly increased leaf chlorophyll index. BR treatments distinctly increased tea polyphenol, catechin, amino acid, and caffeine levels at cold stress, with the greatest effect at 0.1 mg BR/L. Foliar spraying of BR showed no effect on the expression of CsGOGAT at cold stress, but it differentially regulated the expression of CsHMGR, CsGDH, and CsGs, accompanied by their expression being up-regulated under 0.1 mg BR/L treatment. BR-treated plants exhibited a low level of oxidative damage at cold stress based on malondialdehyde levels, which was associated with higher glutathione levels and CsCAT and CsSOD gene expression levels under BR concentrations of 0.05 mg/L and 0.1 mg/L. Non-targeted metabolomics found a total of 26,175 metabolites, the majority of which were lipids and lipid-like molecules (8.97%) and organic heterocyclic compounds (8.97%). BR treatments with 0.05, 0.1, 0.5, and 1 mg/L triggered 1181, 1997, 2414, and 1455 differential metabolites, respectively, accompanied by more differential metabolites being up-regulated. Among them, 18 differential metabolites were associated with tea quality. The enriched pathways of differential metabolites were mainly caffeine metabolism, amino acid synthesis and metabolism, alkaloid synthesis and metabolism, and flavonoid synthesis, depending on the BR concentrations used. Caffeine metabolism was an BR-inducible differential metabolite pathway. Taken together, foliar spraying of BR (0.1 mg/L) improved leaf antioxidant capacity and quality as well as modulated secondary metabolites and their pathways in cold-stressed tea.

Abstract Background The precise mechanisms of hormone action responsible for the full course of events modulating folliculogenesis in crocodilian have not been determined, although histological features have been identified. Results The Alligator sinensis ovarian morphological characteristics observed at 1, 15, 30, 60, 90, and 300 days post hatching(dph) revealed that the dynamic changes in germ cells varied in different meiotic and developmental stages, confirming that the processes of folliculogenesis were protracted and asynchronous. The presence of endogenous follicle-stimulating hormone(FSH) mRNA and protein expression within the cerebrum at 1 dph, in parallel with the increase in germ cells within the germ cell nests(Nest) from 1 dph to 15 dph, suggested that endocrine regulation of the pituitary-gonad axis is an early event in oogonia division. Furthermore, the endogenous expression of FSH showed a trend of negative feedback augmentation accompanied by the exhaustion of maternal yolk E2 observed at 15 dph. Such significant elevation of endogenous FSH levels was observed to be related to pivotal events in the transition from mitosis to meiosis, as reflected by the proportion of oogonia during premeiosis interphase, with endogenous FSH levels reaching a peak at the earliest time step of 1 dph. In addition, the simultaneous upregulation of premeiotic marker STRA8 mRNA expression and the increase in endogenous FSH further verified the above speculation. The strongly FSHr-positive label in the oocytes within Pre-previtellogenic follicles was synchronized with the significant elevation of ovarian cAMP detected at 300 dph, which suggested that diplotene arrest maintenance during early vitellogenesis might be FSH dependent. In addition, preferential selection in asynchronous meiotic initiation has been supposed to act on somatic supportive cells and not directly on germ cells via regulation of FSH that in turn affects downstream estrogen levels. This suggestion was verified by the reciprocal stimulating effect of FSH and E2 on the accelerated meiotic marker SYCP3 and by the inhibited cell apoptosis demonstrated in ovarian cell culture in vitro. Conclusion The corresponding results contribute an expansion of the understanding of physiological processes and shed some light on the specific factors responsible for gonadotropin function in the early folliculogenesis of crocodilians.

Background: Cardiogenic shock (CS) is the primary cause of death in patients suffering acute myocardial infarction. As an emerging and efficacious therapeutic approach, Chinese herbal injections (CHIs) are gaining significant popularity in China. However, the optimal CHIs for treating CS remain uncertain.Method: We searched eight databases from inception to 30 September 2023. Subsequently, we conducted the Bayesian network meta-analysis (NMA). Interventions were ranked based on the surface under the cumulative ranking curve (SUCRA) probability values. To compare the effects of CHIs on two distinct outcomes, a clustering analysis was performed. Furthermore, the quality of the studies was assessed.Results: For the study, we included 43 RCTs, encompassing 2,707 participants. The study evaluated six herbal injections, namely, Shenfu injection (SF), Shengmai injection (SM), Shenmai injection (Sm), Danshen injection (DS), Huangqi injection (HQ), and Xinmailong injection (XML). The analysis findings suggested that Sm (MD = −1.05, 95% CI: −2.10, −0.09) and SF (MD = −0.81, 95% CI: −1.40, −0.25) showed better efficacy compared to Western medicine (WM) alone in reducing in-hospital mortality. The SUCRA values revealed that Sm + WM ranked first in terms of in-hospital mortality, cardiac index (CI), and hourly urine output but second in improving left ventricular ejection fraction (LVEF) and mean arterial pressure (MAP). SF + WM, however, had the greatest impact on raising the clinical effective rate. In MAP, SM + WM came out on top. Moreover, in terms of safety, only 14 studies (31.8%), including five types of CHIs: SF, Sm, SM, HQ, and XML, observed adverse drug reactions.Conclusion: To summarize, this analysis discovered that, in terms of patients suffering from CS, CHIs + WM yielded significantly greater advantages than WM alone. Based on in-hospital mortality and the remaining outcomes, Sm performed excellently among all the involved CHIs.Systematic Review Registration: https://www.Crd.york.ac.uk/prospero/, identifier: CRD42022347053.

Inflammatory bowel disease (IBD) is a chronic nonspecific inflammatory disease of the gastrointestinal tract, and its pathogenesis has not been fully understood. Extensive dysregulation of the intestinal mucosal immune system is critical in the development and progression of IBD. T helper (Th) 17 cells have the characteristics of plasticity. They can transdifferentiate into subpopulations with different functions in response to different factors in the surrounding environment, thus taking on different roles in regulating the intestinal immune responses. In this review, we will focus on the plasticity of Th17 cells as well as the function of Th17 cells and their related cytokines in IBD. We will summarize their pathogenic and protective roles in IBD under different conditions, respectively, hoping to further deepen the understanding of the pathological mechanisms underlying IBD and provide insights for future treatment.

In this study, the physicochemical properties and antioxidant activity of alkali-extracted soluble dietary fiber (SDF) extracted from Sporisorium reilianum were investigated. According to the single-factor experiment results, the extraction process of SDF was optimized by response surface methodology. Response surface analysis results indicated that the maximum extraction yield of SDF reached up to 20.21% under the optimized conditions with the solid-to-liquid of 1:21.40 g/mL, alkali concentration of 2.11%, alkalinization time of 90.71 min and extraction temperature of 59.30 ℃. Meanwhile, the results showed that the water holding capacity, oil holding capacity and swelling capacity of SDF were 3.48±0.05 g/g, 1.50±0.07 g/g and 13.22±0.03 mL/g, respectively. In addition, the experimental results also showed that SDF had high antioxidant activity and the scavenging rate of free radicals was positively correlated with the concentration of SDF. The concentration of 3.5 mg/mL scavenged 62.02% ·OH, 56.98% DPPH·, and 61.03% O2-·, respectively. These results indicated that Sporisorium reilianum was a potential source of natural dietary fiber and a potential functional food ingredient.

Powder metallurgy superalloy is an advanced high-temperature material, which has been widely used in the aerospace fields. Hot isostatic pressing (HIP) is one of the preparation methods for powder superalloy components. However, the presence of prior particle boundaries (PPBs) adversely affects the performance of the components significantly. The research status of PPBs for powder metallurgy superalloys prepared by HIP was reviewed in this paper, the formation mechanism and influence of PPBs in components were summarized, the removal PPBs methods in powder superalloys were proposed, and the feasibility and effectiveness of these methods were analyzed and prospected, including adding Hf, Nb, and other strong carbide forming elements to the powder, preheating the powder, using the vacuum dynamic degassing treatment or plasma droplet re-fining (PDR) treatment, optimizing the powder preparation process, selecting powders with higher purity and more uniform size distribution, selecting the appropriate HIP process parameters and methods, and applying hot extrusion, annealing, solution treatment and HIP post-treatment on the components.

In order to harness the ample solar energy resources and minimize conflicts in drip irrigation schedules, a field experiment was conducted in Xinjiang, northwest China, during 2020 and 2021. The study examined the combined effects of two irrigation timing (daytime - DI and nighttime - NI) and four water temperatures (15, 20, 25, and 30 °C), resulting in eight treatment combinations. The analysis focused on evaluating the impacts of these factors on soil conditions, cotton phenological period, reproductive growth, and fiber quality. Results showed that elevating the irrigation water temperature had a notable impact on soil respiration throughout the different growth stages. The treatment with an irrigation water temperature of 30 °C exhibited a substantial increase in soil respiration, ranging from 17.92% to 45.95%, when compared to conventional irrigation with water temperature at 15 °C. The elevation of irrigation water temperature resulted in notable reductions in soil nitrate and ammonium nitrogen levels. Specifically, the treatment with irrigation water temperature set at 30 °C exhibited an average decrease of 21.69% and 19.27% in soil nitrate and ammonium nitrogen content, respectively, compared to the 15 °C treatment in both years. Nighttime irrigation with increased water temperatures of 20, 25, and 30 °C advanced the onset of the boll opening stage and stimulated the reproductive growth of cotton. Conversely, irrigation with regular water temperature (15 °C) decreased the number of bolls during the early boll-forming period, as well as and the peak number of squares and flowers. However, it resulted in an increased number of bolls during the late boll-forming period. Surprisingly, despite the enhancement in fiber strength and elongation due to water-warming irrigation, it unexpectedly led to a decrease in fiber length and uniformity. Moreover, higher irrigation water temperatures (25 and 30 °C) even resulted in poor levels (> 5.0) of micronaire. Nevertheless, nighttime warming irrigation improved all other fiber qualities except for uniformity. Therefore, when considering the application of water-warming irrigation in arid regions under mulched drip systems, careful selection of irrigation timing and water temperatures is necessary, taking into account the desired fiber quality. This study serves as a valuable technical reference for such irrigation practices.

While treating sensorimotor impairments, a therapist may provide physical assistance by guiding their patient’s limb to teach a desired movement. In this scenario, a key aspect is the compliance of the interaction, as the therapist can provide subtle cues or impose a movement as demonstration. One approach to studying these interactions involves haptically connecting two individuals through robotic interfaces. Upper-limb studies have shown that pairs of connected individuals estimate one another’s goals during tracking tasks by exchanging haptic information, resulting in improved performance dependent on the ability of one’s partner and the stiffness of the virtual connection. In this study, our goal was to investigate whether these findings generalize to the lower limb during an ankle tracking task. Pairs of healthy participants (i.e., dyads) independently tracked target trajectories with and without connections rendered between two ankle robots. We tested the effects of connection stiffness as well as visual noise to manipulate the correlation of tracking errors between partners. In our analysis, we compared changes in task performance across conditions while tracking with and without the connection. We found that tracking improvements while connected increased with connection stiffness, favoring the worse partner in the dyad during hard connections. We modeled the interaction as three springs in series, considering the stiffness of the connection and each partners’ ankle, to show that improvements were likely due to a cancellation of random tracking errors between partners. These results suggest a simplified mechanism of improvements compared to what has been reported during upper-limb dyadic tracking.

Optimizing skill acquisition during novel motor tasks and regaining lost motor functions have been the interest of many researchers over the past few decades. One approach shown to accelerate motor learning involves haptically coupling two individuals through robotic interfaces. Studies have shown that an individual’s solo performance during upper-limb tracking tasks may improve after haptically-coupled training with a partner. In this study, our goal was to investigate whether these findings can be translated to lower-limb motor tasks, more specifically, during an ankle position tracking task. Using one-degree-of-freedom ankle movements, pairs of participants (i.e., dyads) tracked target trajectories independently. Participants alternated between tracking trials with and without haptic coupling, achieved by rendering a virtual spring between two ankle rehabilitation robots. In our analysis, we compared changes in task performance across trials while training with and without haptic coupling. The tracking performance of both individuals (i.e., dyadic task performance) improved during haptic coupling, which was likely due to averaging of random errors of the dyadic pair during tracking. However, we found that dyadic haptic coupling did not lead to faster individual learning for the tracking task. These results suggest that haptic coupling between unimpaired individuals may not be an effective method of training ankle movements during a simple, one-degree-of-freedom task.

This study aims to evaluate whether a selected arbuscular mycorrhizal fungus, Diversispora spurca, improves growth in drought-stressed walnut (Juglans regia L. cv. Qingxiang) plants and whether this improvement is associated with changes in osmolyte (fructose, glucose, sucrose, soluble protein, proline, and betaine) levels. After 60 days of soil drought treatment (50% of maximum field water-holding capacity), root D. spurca colonization rate and soil mycelium length decreased by 13.57% and 64.03%, respectively. Soil drought also inhibited the growth performance of aboveground (stem diameter, leaf number, leaf biomass, and stem biomass) and underground (root projected area, surface area, and average diameter) parts, with uninoculated plants showing a stronger inhibition than D. spurca-inoculated plants. D. spurca significantly increased these growth variables, along with aboveground part variables and root areas being more prominent under drought stress versus non-stress conditions. Although drought treatment suppressed the chlorophyll index and nitrogen balance index in leaves, mycorrhizal inoculation significantly increased these indices. Walnut plants were able to actively increase leaf fructose, glucose, sucrose, betaine, and proline levels under such drought stress. Inoculation of D. spurca also significantly increased leaf fructose, glucose, sucrose, betaine, proline, and soluble protein levels under drought stress and non-stress, with the increasing trend in betaine and soluble protein being higher under drought stress versus non-stress. Drought stress dramatically raised leaf hydrogen peroxide (H2O2) levels in both inoculated and uninoculated plants, while mycorrhizal plants presented significantly lower H2O2 levels, with the decreasing trend higher under drought stress versus non-stress. In conclusion, D. spurca symbiosis can increase the growth of drought-stressed walnut plants, associated with increased osmolyte levels and decreased H2O2 levels.

Capillary leak syndrome (CLS) is a serious disorder characterized by hypotension and refractory systemic oedema. CLS with marked ascites rather than systemic oedema is rare and prone to misdiagnosis and delayed treatment. We report here a case of marked ascites in an elderly male patient with hepatitis B virus reactivation. Following investigations to exclude common diseases that may have accounted for diffuse oedema and hypercoagulable state, anti-cirrhosis therapy failed and severe refractory shock developed 48 hours after admission. The patient developed mild pleural effusions followed by swelling of the face, neck, and extremities. A high cytokine concentration gradient was detected between serum and ascites. Peritoneal biopsy showed lymphoma cells. The final diagnosis was lymphoma recurrence complicated with CLS. Our case suggests that cytokine detection in serum and ascitic fluid may be helpful in the differential diagnosis of CLS. In similar cases, decisive intervention, such as, hemodiafiltration, should be implemented to lessen the likelihood of serious complications.

Osteochondral defects are the main cause of joint morbidity and disability in elderly patients, and osteochondral tissue engineering is one of the methods to repair osteochondral defects. The method of osteochondral tissue engineering involves the manufacture of osteochondral biomimetic gradient scaffolds that should mimic the physiological properties of natural osteochondral tissue (e.g., the gradient transition between cartilage surface and subchondral bone). The osteochondral biomimetic gradient scaffolds exhibit discrete gradients or continuous gradients to establish the characteristics of osteochondral tissue in many studies, such as biochemical composition, structure and mechanical properties. An advantage of the continuous osteochondral biomimetic gradient scaffold is that there is no obvious interface between each layer, therefore it more closely mimics the natural osteochondral tissue. Although promising results have been achieved so far on the regeneration of the osteochondral biomimetic gradient scaffold, there are still differences between the osteochondral biomimetic gradient scaffold and natural osteochondral tissue. Due to these differences, the current clinical treatment of osteochondral biomimetic gradient scaffolds to repair osteochondral defects needs further research. Firstly, the research progress on discrete and continuous gradient scaffolds from the background of osteochondral defects, the micro-scale structure and mechanical properties of osteochondral to the materials and methods related to the manufacture of osteochondral biomimetic gradient scaffolds was summarized in this article. Secondly, due to the 3D printing method of the osteochondral biomimetic gradient scaffold having the ability to precisely control the geometry of the scaffold hole and the mechanical properties of the scaffold, the application of computational simulation models in osteochondral tissue engineering was further introduced, for example, optimizing scaffold structure and mechanical properties are considered to predict tissue regeneration. Finally, the challenges related to the repair of osteochondral defects and prospects for the future research of osteochondral tissue regeneration were presented.For example, continuous osteochondral bionic gradient scaffolds need to more similarly simulate the structure of natural osteochondral tissue units, that is, the transition of mechanical properties and biochemical properties is more smooth naturally. At the same time, although most osteochondral biomimetic gradient scaffolds have achieved good results in in vivo and in vitro experiments, clinical research and application still need to be further studied.

Objective: Previous studies have demonstrated promising results in estimating the neural drive to muscles, the net output of all motoneurons that innervate the muscle, using high-density electromyography (HD-EMG) for the purpose of interfacing with assistive technologies. Despite the high estimation accuracy, current methods based on neural networks need to be trained with specific motor unit action potential (MUAP) shapes updated for each condition (i.e., varying muscle contraction intensities or joint angles). This preliminary step dramatically limits the potential generalization of these algorithms across tasks. We propose a novel approach to estimate the neural drive using a deep convolutional neural network (CNN), which can identify the cumulative spike train (CST) through general features of MUAPs from a pool of motor units. Methods: We recorded HD-EMG signals from the gastrocnemius medialis muscle under three isometric contraction scenarios: 1) trapezoidal contraction tasks with different intensities, 2) contraction tasks with a trapezoidal or sinusoidal torque target, and 3) trapezoidal contraction tasks at different ankle angles. We applied a convolutive blind source separation (BSS) method to decompose HD-EMG signals to CST and segmented both signals into windows to train and validate the deep CNN. Then, we optimized the structure of the deep CNN and validated its generalizability across contraction tasks within each scenario. Results: With the optimal configuration for the HD-EMG data window (overlap of 20 data points and window length of 40 data points), the deep CNN estimated the CST close to that from BSS, with a correlation coefficient higher than 0.96 and normalized root-mean-square-error lower than 7% with respect to the BSS (golden standard) within each scenario. Conclusion: The proposed deep CNN framework can utilize data from different contraction tasks (e.g., different intensities), learn general features of MUAP variants, and estimate the neural drive for other contraction tasks. Significance: With the proposed deep CNN, we could potentially build a neural-drive-based human-machine interface that is generalizable to different contraction tasks without retraining.

Abstract Background Parkinson’s disease (PD) is characterized by degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc), accompanied by accumulation of α-synuclein, chronic neuroinflammation and autophagy dysfunction. Previous studies suggested that misfolded α-synuclein induces the inflammatory response and autophagy dysfunction in microglial cells. The NLRP3 inflammasome signaling pathway plays a crucial role in the neuroinflammatory process in the central nervous system. However, the relationship between autophagy deficiency and NLRP3 activation induced by α-synuclein accumulation is not well understood. Methods Through immunoblotting, immunocytochemistry, immunofluorescence, flow cytometry, ELISA and behavioral tests, we investigated the role of p38-TFEB-NLRP3 signaling pathways on neuroinflammation in the α-synuclein A53T PD models. Results Our results showed that increased protein levels of NLRP3, ASC, and caspase-1 in the α-synuclein A53T PD models. P38 is activated by overexpression of α-synuclein A53T mutant, which inhibited the master transcriptional activator of autophagy TFEB. And we found that NLRP3 was degraded by chaperone-mediated autophagy (CMA) in microglial cells. Furthermore, p38-TFEB pathways inhibited CMA-mediated NLRP3 degradation in Parkinson's disease. Inhibition of p38 had a protective effect on Parkinson's disease model via suppressing the activation of NLRP3 inflammasome pathway. Moreover, both p38 inhibitor SB203580 and NLRP3 inhibitor MCC950 not only prevented neurodegeneration in vivo, but also alleviated movement impairment in α-synuclein A53T-tg mice model of Parkinson’s disease. Conclusion Our research reveals p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease, which could be a potential therapeutic strategy for PD. Graphical abstract p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease. In this model, p38 activates NLRP3 inflammasome via inhibiting TFEB in microglia. TFEB signaling negatively regulates NLRP3 inflammasome through increasing LAMP2A expression, which binds to NLRP3 and promotes its degradation via chaperone-mediated autophagy (CMA). NLRP3-mediated microglial activation promotes the death of dopaminergic neurons.

Abstract Background Human-human (HH) interaction mediated by machines (e.g., robots or passive sensorized devices), which we call human-machine-human (HMH) interaction, has been studied with increasing interest in the last decade. The use of machines allows the implementation of different forms of audiovisual and/or physical interaction in dyadic tasks. HMH interaction between two partners can improve the dyad’s ability to accomplish a joint motor task (task performance) beyond either partner’s ability to perform the task solo. It can also be used to more efficiently train an individual to improve their solo task performance (individual motor learning). We review recent research on the impact of HMH interaction on task performance and individual motor learning in the context of motor control and rehabilitation, and we propose future research directions in this area. Methods A systematic search was performed on the Scopus, IEEE Xplore, and PubMed databases. The search query was designed to find studies that involve HMH interaction in motor control and rehabilitation settings. Studies that do not investigate the effect of changing the interaction conditions were filtered out. Thirty-one studies met our inclusion criteria and were used in the qualitative synthesis. Results Studies are analyzed based on their results related to the effects of interaction type (e.g., audiovisual communication and/or physical interaction), interaction mode (collaborative, cooperative, co-active, and competitive), and partner characteristics. Visuo-physical interaction generally results in better dyadic task performance than visual interaction alone. In cases where the physical interaction between humans is described by a spring, there are conflicting results as to the effect of the stiffness of the spring. In terms of partner characteristics, having a more skilled partner improves dyadic task performance more than having a less skilled partner. However, conflicting results were observed in terms of individual motor learning. Conclusions Although it is difficult to draw clear conclusions as to which interaction type, mode, or partner characteristic may lead to optimal task performance or individual motor learning, these results show the possibility for improved outcomes through HMH interaction. Future work that focuses on selecting the optimal personalized interaction conditions and exploring their impact on rehabilitation settings may facilitate the transition of HMH training protocols to clinical implementations.

Objective To exam the time trend of the prevalence of metabolically healthy obesity (MHO) in the US adult population.Design Eight cross-sectional survey cycles.Setting National Health and Nutrition Examination Survey (NHANES), 1999–2014.Participants 16 459 NHANES participants aged 20 years and older.Primary outcome measure MHO was defined as central obesity (waist circumference ≥102 cm for men and ≥88 cm for women) without any of the following conditions: elevated levels of blood pressure (≥130/85 mm Hg), glucose (≥100 mg/dL) and triglycerides (≥150 mm/dL); reduced levels of high-density lipoprotein cholesterol (

BackgroundThere is a relative lack of data that systematically investigates the breadth and validity of the association between bariatric surgery and health-related outcomes. We aimed to evaluate the quantity, validity, and credibility of evidence regarding the association between bariatric surgery and health-related outcomes using an umbrella review of meta-analyses.MethodsWe systematically searched PubMed, Embase, and the Web of Science databases from inception until December 2, 2021, to identify meta-analyses of observational or interventional studies that investigated the association between bariatric surgery and multiple health outcomes. We extracted the summary effect size and 95% confidence interval (CI) data. The Assessment of Multiple Systematic Reviews (AMSTAR-2) and Grading of Recommendations, Assessment, Development, and Evaluations (GRADE) guidelines were used for methodological and evidence quality assessments, respectively.ResultsTwenty-eight studies with 82 different health-related outcomes were included in this umbrella review. Beneficial effects of bariatric surgery have been observed in cancer incidence, mortality, cardiovascular risk, polycystic ovary syndrome (PCOS), anxiety symptoms, depressive symptoms, gestational diabetes mellitus, gestational hypertension, large for gestational age (LGA), macrosomia, post-term birth, risk of kidney stones, albuminuria, urinary incontinence, fecal incontinence, Barrett’s esophagus, and diabetic retinopathy. However, adverse effects of bariatric surgery were observed for maternal anemia, perinatal mortality, congenital anomalies, preterm birth, neonatal intensive care unit (NICU) admission, intrauterine growth restriction, small for gestational age (SGA), fracture risk, upper limb fracture, suicide, self-harm, and alcohol use disorder (AUD).ConclusionsCurrent evidence suggests that bariatric surgery improves the majority of health-related outcomes; however, caution is advised given it may increase the risk of adverse mental effects, perinatal problems, and fractures.

Large-fruit bud mutations are important factors in fruit tree breeding. However, little is known about the differences between varieties and bud mutations. The ploidy identification of Korla fragrant pear (Pyrus sinkiangensis Yu) and its large bud mutation Zaomeixiang pear showed that the large-fruit characteristic was not caused by chromosome doubling. By counting mesocarp cells at different stages, we found that the number of cells increased continuously after pollination, and the difference was the greatest at 28 days after full bloom (DAFB), and was about 9.4 × 106. After 28 days, the difference in cell volume became bigger and bigger, so both the cell volume and cell number caused the difference in fruit size between Korla fragrant pear and Zaomeixiang pear. To obtain more insights into the differences in fruit size driven by cell division, we analyzed the endogenous hormones [indole ascetic acid (IAA), zeatin riboside (ZR), gibberellic acid (GA), and abscisic acid (ABA)], and the main sugars (glucose, fructose, sucrose, and sorbitol). The ZR content of Zaomeixiang pear was always greater than that of Korla fragrant pear at all stages. The ABA content was the opposite except for at 7 DAFB during cell division; the greatest difference was 30.87 ng/g, which appeared at 28 DAFB. ABA and ZR correlated negatively with cell number. After 7 DAFB, the ratio of IAA/ABA, ZR/ABA, and GA/ABA in Zaomeixiang pear was always greater than that for Korla fragrant pear at 28 DAFB. The difference in glucose content at 21 DAFB was the greatest, at 4.80 ng/g. Large amounts of sorbitol accumulated during whole-cell division. Glucose and sorbitol correlated positively with cell numbers. In summary, the data suggest that the different contents of glucose, sorbitol, ZR, and ABA, and the ratio of endogenous hormones might be related to cell division in Korla fragrant pear and Zaomeixiang pear. The result provides a theoretical basis for the large-size fruit’s high-quality production and genetic breeding of Korla fragrant pear and its bud mutation.

An experiment was conducted from 2016 to 2017 to assess the effect of kernel metabolism in development stages after organic mulching compared to control. Organic mulching significantly increased crop yields (higher 128% in 2016, higher 60% in 2017), oil content (the highest oil content was 27.6% higher than that of the control), and improved soil properties (SOC, SAN, AP, and AK). In this study, soil pH, SOC, AN, AP, and AK in 0–30 cm soil depth were measured. Results showed that the effect of mulching on soil pH was not significant at the harvesting stage. The greatest metabolic differences occurred during the period of high oil conversion (S2–S4), primarily involving 11 relevant metabolic pathways. This further verified that Camellia oleifera oil yield was improved after mulching. A total of 1,106 OTUs were detected by using 16S rRNA, and Venn diagram showed that there were 106 unique OTUs in control and 103 OTUs in the treatment, respectively. Correlation analysis showed that soil pH and soil temperature were two indicators with the most correlations with soil microbiota. The yield was significantly positively correlated with soil microbial Proteobacteria, Bacteroidetes, and soil nutrition indexes. Organic mulching improved the physicochemical properties of soils, caused differences in the relative abundance of dominant bacteria in soil bacteria, and improved the soil microbiological environment to promote plant growth, indicating that organic mulching is an effective measure to alleviate seasonal drought.

Abstract To evaluate the effect of photobiomodulation therapy (PBMT) on painful temporomandibular disorders (TMD) patients in a randomized, double-blinded, placebo-controlled manner. Participants were divided into a masseter myalgia group (n = 88) and a temporomandibular joint (TMJ) arthralgia group (n = 87) according to the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD). Both groups randomly received PBMT or placebo treatment once a day for 7 consecutive days, one session. The PBMT was applied with a gallium-aluminum-arsenide (GaAlAs) laser (wavelength = 810 nm) at pre-determined points in the masseter muscle (6 J/cm2, 3 regions, 60 s) or TMJ region (6 J/cm2, 5 points, 30 s) according to their most painful site. Pain intensity was rated on a 0–10 numerical rating scale (NRS) and pressure pain thresholds (PPT) and mechanical sensitivity mapping were recorded before and after the treatment on day 1 and day 7. Jaw function was assessed by pain free jaw opening, maximum unassisted jaw opening, maximum assisted jaw opening, maximum protrusion and right and left excursion. Data were analyzed with a mixed model analysis of variance (ANOVA). Pain intensity in arthralgia patients decreased over time (P

Fruit size is a major factor determining yield, quality, and consumer acceptability. fw2.2 (fruit weight-2.2) is a primary quantitative trait locus that was the first to be cloned, accounting for 30% of the variation in tomato fruit size. The various homologs of fw2.2 (fw2.2-like) have been identified in many plants and belong to a large family. To date, there has been no report that has carried out a comprehensive identification of fw2.2-like members in pear. In this study, a total of 14 fw2.2-like genes were identified in the pear (Pyrus bretschneideri Rehd) genome and designated as PbFWL1-14. All of the PbFWL genes were unevenly distributed on nine chromosomes, and each chromosome contained between one and four genes. All PbFWL proteins contained more than two conserved motifs, and PbFWL genes contained more than one intron, and the genes of the same subfamily seemed to have a similar intron gene structure. According to the neighbor-joining phylogenetic tree, a total of 78 fw2.2/CNR (cell number regulator) from five plant species, including pear, maize, tomato, peach, rice, and physalis, could be divided into seven subgroups, and PbFWL proteins were mainly distributed in subgroups 1, 3, 4, 5, and 6. The Ka/Ks analysis also revealed that the fw2.2-like gene family of pear may have been subjected to strong purifying selection pressure during its evolution. A cis-element analysis found that many cis-elements responsive to hormones and stress were discovered in promotion regions for all PbFWLs. When combining real-time quantitative PCR analysis detection results, PbFWL1/2/5 were found to be the most likely candidate genes for regulating pear fruit size.

The periods of flower bud differentiation and fruit growth for Camellia oleifera overlap greatly affect the allocation of photoassimilates to flower buds and fruit, resulting in obvious alternate bearing. To export the cause and mitigate alternate bearing of Camellia oleifera, the allocation of photoassimilates to buds and fruit supplied by leaves at different node positions was studied by the addition of labeled 13CO2 during the slow fruit growth stage. The fate of 13C photoassimilated carbon was followed during four periods: slow fruit growth (4 hours and 10 days after 13C labeling); rapid growth (63 days after 13C labeling); oil conversion (129 days after 13C labeling); and maturation (159 days after 13C labeling). Photosynthetic parameters and leaf areas of the leaves shared a common pattern (fifth > third > first), and the order of photosynthetic parameters of different fruit growth stages was as follows: oil conversion > maturation > rapid growth > slow growth. The most intense competition between flower bud differentiation and fruit growth occurred during the oil conversion stage. Dry matter accumulation in different sinks occurred as follow: fruit > flower bud > leaf bud. Photoassimilates from the labeled first leaf were mainly translocated to the first flower bud, and the upper buds were always differentiated into flower buds. The photoassimilates from the labeled third leaf were distributed disproportionately to the third flower bud and fruit. They distributed more to the third flower bud, and the middle buds formed either flower or leaf buds. However, the photoassimilates from the labeled fifth leaf were primarily allocated to the fruit that bore on the first node of last year’s bearing shoot, and basal buds did not form flower buds. Based on our results, the basal leaves should be retained for a high yield in the current year, and the top leaves should be retained for a high yield in the following year. Our results have important implications for understanding the management of flower and fruit in C. oleifera. The thinning of fruit during the on-crop year can promote flower bud formation and increase the yield of C. oleifera crops in the following year. During the off-year, more fruit should be retained to maintain the fruit yield. The thinning of middle-upper buds could promote more photoassimilates allocate to the fruit.

The drug supervision methods based on near-infrared spectroscopy analysis are heavily dependent on the chemometrics model which characterizes the relationship between spectral data and drug categories. The preliminary application of convolution neural network in spectral analysis demonstrates excellent end-to-end prediction ability, but it is sensitive to the hyper-parameters of the network. The transformer is a deep-learning model based on self-attention mechanism that compares convolutional neural networks (CNNs) in predictive performance and has an easy-to-design model structure. Hence, a novel calibration model named SpectraTr, based on the transformer structure, is proposed and used for the qualitative analysis of drug spectrum. The experimental results of seven classes of drug and 18 classes of drug show that the proposed SpectraTr model can automatically extract features from a huge number of spectra, is not dependent on pre-processing algorithms, and is insensitive to model hyperparameters. When the ratio of the training set to test set is 8:2, the prediction accuracy of the SpectraTr model reaches 100% and 99.52%, respectively, which outperforms PLS_DA, SVM, SAE, and CNN. The model is also tested on a public drug data set, and achieved classification accuracy of 96.97% without pre-processing algorithm, which is 34.85%, 28.28%, 5.05%, and 2.73% higher than PLS_DA, SVM, SAE, and CNN, respectively. The research shows that the SpectraTr model performs exceptionally well in spectral analysis and is expected to be a novel deep calibration model after Autoencoder networks (AEs) and CNN.

The wear resistance of TC4 titanium alloy was improved by plasma nitriding and the optimum nitriding temperature was investigated. TC4 titanium alloy was nitrided at 650, 700, 750, 800, 850℃ and 900℃ with LDM1-100 plasma nitriding equipment respectively,the temperature holding time is 10 h for each nitriding treatment. The microstructure, surface morphology, surface roughness, phase structure and hardness of nitrided samples at different temperatures were characterized by optical microscope, scanning electron microscope, white light three-dimensional topography instrument and microhardness tester. The tribological properties of TC4 titanium alloy after plasma nitriding were tested by CETR UMT-3 multifunctional friction and wear tester. The results show that the surface microhardness and roughness of TC4 titanium alloy increase with the increase of temperature. After nitriding at 900℃, the surface microhardness of TC4 titanium alloy reaches 1318HV0.05, which is about 4 times as high as that of the substrate (360HV0.05). The increase of hardness is due to the formation of hard nitride phases (TiN and Ti2N phases) on the surface of nitrided sample and the nitride content increases with the increase of nitriding temperature. The load-carrying capacity of nitrided samples at 800℃ and 900℃ is significantly higher than that of nitriding samples at low temperatures (below 750℃). Compared with the original TC4 sample, the wear volume of the nitrided sample significantly reduces. When nitriding temperature is 850℃, the wear volume of the nitrided sample is 1.2% (1 N), 3.0% (3 N) and 62.2% (5 N) of that of the untreated sample, and the improvement of wear resistance of the sample is the most obvious.

Significant advances have been made in the morphological observations of the dorsal gland (DG), an oval organ/tissue which lies on both sides of the dorsal midline of the crocodilian. In the current study, RNA sequencing (RNA-seq) was used to identify the changing patterns of Alligator sinesis DGs at different timepoints from the 31st embryonic day (E31) to the newly hatched 1st day (NH1). A comprehensive transcriptional changes of differentially expression gene (DEGs) involved in the melanogenesis, cholesterol metabolism, and cell apoptosis pathways suggested that the DG might serves as a functional secretory gland in formation, transport and deposition of pigment, and lipids secretion via lysosomal exocytosis. Furthermore, the remarkable immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and B-cell lymphoma 2 (Bcl-2)-positive signals in the basilar cells, in parallel with the immuno-reactive TdT-mediated dUTP nick-End labeling(TUNEL) within suprabasal cells, provided direct molecular evidence supporting for the speculation that DG serves as a holocrine secretion mode. Finally, subsequent phylogenetic and immunohistochemical analysis for the PITX2, the identified DEGs in the RNA-seq, was helpful to further elucidate the transcriptional regulatory mechanism of candidate genes. In conclusion, the current results are of considerable importance in enriching our understanding of the intrinsic relationship between the skin derivatives and lifestyles of newborn Alligator sinesis.

Objectives This study was to determine the incidence of falls and identify baseline factors increased risk for incident falls over time among people with diabetes.Design This study was a secondary analysis using the baseline and 4 years of follow-up data from the China Health and Retirement Longitudinal Study (CHARLS).Setting A nationally representative survey of 17 500 Chinese residents aged 45 years and older were recruited in the baseline national survey in 2011. These participants were followed up every 2 years.Participants A total of 1238 middle-aged and older adults with diabetes and no history of falls at baseline were included in the current study.Primary and secondary outcome measures Information on incidence of falls and medical treatment resulting from falls were determined by self-report.Results The findings showed that the incidence of falls was 29.4% during 4 years of follow-up. Participants with incident falls were younger, were more likely to be women, had lower education level and were less likely to be current drinkers. In addition, former drinkers were 2.22 times more likely to fall. Socially active individuals were 47% less likely to fall compared with those without social activities. Every 5 kg increase in grip strength was associated with a 13% lower risk of falls. A 10 mg/dL higher total cholesterol and 1 mg/dL higher blood urea nitrogen were associated with a 4% and 6% higher risk of falls. Finally, participants with depressive symptoms were 1.47 times more likely to fall compared with those without depressive symptoms.Conclusions These findings underscore the importance of developing a fall prevention programme for those with diabetes, and this programme should address potentially modifiable risk factors, including levels of total cholesterol, blood urea nitrogen, social activity, depressive symptoms and grip strength.

Abstract Background ERp57 dysfunction has been shown to contribute to tumorigenesis in multiple malignances. However, the role of ERp57 in clear cell renal carcinoma (ccRCC) remains unclear. Methods Cell proliferation ability was measured by MTT and colony forming assays. Western blotting and quantitative real-time PCR (qRT-PCR) were performed to measure protein and mRNA expression. Co-immunoprecipitation (CoIP) and proximity ligation assay (PLA) were performed to detect protein-protein interaction. Chromatin immunoprecipitation (ChIP), ribonucleoprotein immunoprecipitation (RIP), and oligo pull-down were used to confirm DNA–protein and RNA–protein interactions. Promoter luciferase analysis was used to detect transcription factor activity. Results Here we found ERp57 was overexpressed in ccRCC tissues, and the higher levels of ERp57 were correlated with poor survival in patients with ccRCC. In vivo and in vitro experiments showed that ccRCC cell proliferation was enhanced by ERp57 overexpression and inhibited by ERp57 deletion. Importantly, we found ERp57 positively regulated ILF3 expression in ccRCC cells. Mechanically, ERp57 was shown to bind to STAT3 protein and enhance the STAT3-mediated transcriptional activity of ILF3. Furthermore, ILF3 levels were increased in ccRCC tissues and associated with poor prognosis. Interestingly, we revealed that ILF3 could bind to ERp57 and positively regulate its expression by enhancing its mRNA stability. Furthermore, ccRCC cell proliferation was moderated via the ERp57/STAT3/ILF3 feedback loop. Conclusions In summary, our results indicate that the ERp57/STAT3/ILF3 feedback loop plays a key role in the oncogenesis of ccRCC and provides a potential therapeutic target for ccRCC treatment.

Abstract. B lymphocytes produce antibodies under the stimulation of specific antigens, thereby exerting an immune effect. B cells identify antigens by their surface B cell receptor (BCR), which upon stimulation, directs the cell to activate and differentiate into antibody generating plasma cells. Activation of B cells via their BCRs involves signaling pathways that are tightly controlled by various regulators. In this review, we will discuss three major BCR mediated signaling pathways (the PLC-γ2 pathway, PI3K pathway and MAPK pathway) and related regulators, which were roughly divided into positive, negative and mutual-balanced regulators, and the specific regulators of the specific signaling pathway based on regulatory effects.

In this study, we systematically evaluated the antibiofouling behavior of quaternary ammonium compound (QAC) blended polyvinylidene fluoride (PVDF) membrane using quartz crystal microbalance with dissipation monitoring (QCM-D) combined with flow cytometry (FCM) and confocal laser scanning microscopy (CLSM) measurements. QCM-D tests showed that the introduction of QAC reduced bacterial attachment due to the biocidal functions of QAC. FCM indicated that cell integrity of the bacteria in the suspension flowing along QAC-modified membrane surfaces during the QCM-D test was severely affected. CLSM confirmed the significantly lower attachment of bacteria and higher dead/live cell ratio onto the surface of modified membranes after the washing step in QCM-D tests. Both FCM and CLSM results validated the antibacterial behavior of QAC-modified membranes by a contact-killing mechanism, which is in agreement with that of QCM-D tests. In addition, the bacterial cells accumulated on modified membrane surface exhibited higher reversibility compared to the control membrane, indicating ease of membrane cleaning. The results highlight that the combined use of QCM-D, FCM, and CLSM can comprehensively characterize the antibiofouling behavior of membranes.

The wear resistance of TC4 titanium alloy was improved by plasma nitriding and the optimum nitriding temperature was investigated. TC4 titanium alloy was nitrided at 650, 700, 750, 800, 850℃ and 900℃ with LDM1-100 plasma nitriding equipment respectively,the temperature holding time is 10 h for each nitriding treatment. The microstructure, surface morphology, surface roughness, phase structure and hardness of nitrided samples at different temperatures were characterized by optical microscope, scanning electron microscope, white light three-dimensional topography instrument and microhardness tester. The tribological properties of TC4 titanium alloy after plasma nitriding were tested by CETR UMT-3 multifunctional friction and wear tester. The results show that the surface microhardness and roughness of TC4 titanium alloy increase with the increase of temperature. After nitriding at 900℃, the surface microhardness of TC4 titanium alloy reaches 1318HV0.05, which is about 4 times as high as that of the substrate (360HV0.05). The increase of hardness is due to the formation of hard nitride phases (TiN and Ti2N phases) on the surface of nitrided sample and the nitride content increases with the increase of nitriding temperature. The load-carrying capacity of nitrided samples at 800℃ and 900℃ is significantly higher than that of nitriding samples at low temperatures (below 750℃). Compared with the original TC4 sample, the wear volume of the nitrided sample significantly reduces. When nitriding temperature is 850℃, the wear volume of the nitrided sample is 1.2% (1 N), 3.0% (3 N) and 62.2% (5 N) of that of the untreated sample, and the improvement of wear resistance of the sample is the most obvious.