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Abstract Cultivation of bovine mammary epithelial cells (bMECs) is regarded as a potential future source of milk constituents; however, there is a research gap concerning the investigation of compositional similarity between such secretomes and milk, and furthermore, how culture conditions can be optimized to make it a feasible strategy for potential production of milk constituents. To perform such an initial, detailed quantitative proteomic study of secretomes from in vitro grown bMECs, these cells were isolated from the mammary tissue of two lactating cows and cultured on Matrigel®-coated inserts in a trans-well system. Cells were treated with or without prolactin at the basolateral sides, and the secretomes together with media from the apical side were collected and subsequently compositionally characterized for their protein contents and label-free quantitative proteomics. Using sensitive nLC-timsTOF Pro MS/MS, more than 500 proteins were identified, though in very low total concentrations, across proteomes from the four secretomes collected from prolactin- or non-prolactin-treated cells. The proteins included many cellular proteins and characteristic milk proteins, but with caseins accounting for less than 1% of total protein abundance. Secretomes from prolactin-treated bMECs showed significant (P

Mammary epithelial cells (MEC) are responsible for milk synthesis in the bovine mammary gland during lactation, and could thereby potentially be used for in-vitro milk components production. Development of such a model, however, requires the MECs to preserve their lactogenic traits following in-vitro growth. Potential impact of growing MEC in the lab following their isolation was studied in relation to their population homogeneity and milk synthesis related gene expressions. Upon isolation, flow cytometry results showed that > 30 % of the obtained cells were positive for cytokeratin 18 (CK18), a marker for the MEC. This proportion increased to 95 % MEC after the first passage. Cell functionality was examined by comparing the expressions of milk synthesis related genes at passage 2 (P2) to passage 6 (P6) following treatment with lactogenic agents. CSN1S1 was found to be expressed significantly higher at P2 compared to P6. In addition, CSN2, CSN3 and PRLR tended to have lower expressions in P6 compared to P2. DGAT1, STAT5A, AKT1, and RPS6KB1 were expressed comparably in both passages. The expressions of LALBA and CSN1S2 were not detectable in any of the samples. Therefore, despite having high levels of CK18 positive cells at later passages, in line with other studies, further passaging affected the expressions of some of the genes related to milk synthesis. A reduced gene expression capacity in the propagated MEC may limit their potential for in-vitro milk components production.

The UltraViolet imaging of the Cosmic Assembly Near-infrared Deep Extragalactic Legacy Survey Fields (UVCANDELS) program provides deep Hubble Space Telescope (HST) F275W and F435W imaging over four CANDELS fields (GOODS-N, GOODS-S, COSMOS, and Extended Groth Strip). We combine this newly acquired UV imaging with existing HST imaging from CANDELS as well as existing ancillary data to obtain robust photometric redshifts and reliable estimates for galaxy physical properties for over 150,000 galaxies in the ∼430 arcmin ^2 UVCANDELS area. Here, we leverage the power of the new UV photometry to not only improve the photometric redshift measurements in these fields, but also constrain the full redshift probability distribution combining multiple redshift-fitting tools. Furthermore, using the full UV-to-IR photometric data set, we measure the galaxy physical properties by fitting templates from population synthesis models with two different parameterizations (flexible and fixed form) of the star formation histories (SFHs). Compared to the flexible SFH parameterization, we find that the fixed-form SFHs systematically underestimate the galaxy stellar masses, both at the low-mass (≲10 ^9 M _⊙ ) and high-mass (≳10 ^10 M _⊙ ) end, by as much as ∼0.5 dex. This underestimation is primarily due the limited ability of fixed-form SFH parameterization to simultaneously capture the chaotic nature of star formation in these galaxies.

UVCANDELS is a Hubble Space Telescope Cycle-26 Treasury Program awarded 164 orbits of primary ultraviolet (UV) F275W imaging and coordinated parallel optical F435W imaging in four CANDELS fields—GOODS-N, GOODS-S, EGS, and COSMOS—covering a total area of ∼426 arcmin ^2 . This is ∼2.7 times larger than the area covered by previous deep-field space UV data combined, reaching a depth of about 27 and 28 ABmag (5 σ in 0.”2 apertures) for F275W and F435W, respectively. Along with new photometric catalogs, we present an analysis of the rest-frame UV luminosity function (LF), relying on our UV-optimized aperture photometry method, yielding a factor of 1.5 increase over H-isophot aperture photometry in the signal-to-noise ratios of galaxies in our F275W imaging. Using well-tested photometric redshift measurements, we identify 5810 galaxies at redshifts 0.6 < z < 1, down to an absolute magnitude of M _UV = −14.2. In order to minimize the effect of uncertainties in estimating the completeness function, especially at the faint end, we restrict our analysis to sources above 30% completeness, which provides a final sample of 4726 galaxies at −21.5 < M _UV < −15.5. We performed a maximum likelihood estimate to derive the best-fit parameters of the UV LF. We report a best-fit faint-end slope of $\alpha =-{1.359}_{-0.041}^{+0.041}$ at z ∼ 0.8. Creating subsamples at z ∼ 0.7 and z ∼ 0.9, we observe a possible evolution of α with redshift. The unobscured UV luminosity density at M _UV < −10 is derived as ${\rho }_{{\rm{U}}{\rm{V}}}={1.339}_{-0.030}^{+0.027}\,(\times {10}^{26}\,{\rm{e}}{\rm{r}}{\rm{g}}\,{{\rm{s}}}^{-1}\,{{\rm{H}}{\rm{z}}}^{-1}\,{{\rm{M}}{\rm{p}}{\rm{c}}}^{-3})$ using our best-fit LF parameters. The new F275W and F435 photometric catalogs from UVCANDELS have been made publicly available on the Barbara A. Mikulski Archive for Space Telescopes.

We use the Ultraviolet Imaging of the Cosmic Assembly Near-infrared Deep Extragalactic Legacy Survey fields (UVCANDELS) to measure half-light radii in the rest-frame far-UV for ∼16,000 disk-like galaxies over 0.5 ≤ z ≤ 3. We compare these results to rest-frame optical sizes that we measure in a self-consistent way and find that the stellar mass–size relation of disk galaxies is steeper in the rest-frame UV than in the optical across our entire redshift range. We show that this is mainly driven by massive galaxies (≳10 ^10 M _⊙ ), which we find to also be among the most dusty. Our results are consistent with the literature and have commonly been interpreted as evidence of inside-out growth wherein galaxies form their central structures first. However, they could also suggest that the centers of massive galaxies are more heavily attenuated than their outskirts. We distinguish between these scenarios by modeling and selecting galaxies at z = 2 from the VELA simulation suite in a way that is consistent with UVCANDELS. We show that the effects of dust alone can account for the size differences we measure at z = 2. This indicates that, at different wavelengths, size differences and the different slopes of the stellar mass–size relation do not constitute evidence for inside-out growth.

To study the role of environment in galaxy evolution, we reconstruct the underlying density field of galaxies based on COSMOS2020 (The Farmer catalog) and provide the density catalog for a magnitude-limited ( K _s < 24.5) sample of ∼210,000 galaxies at 0.4 < z < 5 within the COSMOS field. The environmental densities are calculated using a weighted kernel density estimation approach with the choice of a von Mises–Fisher kernel, an analog of the Gaussian kernel for periodic data. Additionally, we make corrections for the edge effect and masked regions in the field. We utilize physical properties extracted by LePhare to investigate the connection between star formation activity and the environmental density of galaxies in six mass-complete subsamples at different cosmic epochs within 0.4 < z < 4. Our findings confirm a strong anticorrelation between star formation rate (SFR)/specific SFR (sSFR) and environmental density out to z ∼ 1.1. At 1.1 < z < 2, there is no significant correlation between SFR/sSFR and density. At 2 < z < 4, we observe a reversal of the SFR/sSFR–density relation such that both SFR and sSFR increase by a factor of ∼10 with increasing density contrast, δ , from −0.4 to 5. This observed reversal at higher redshifts supports the scenario where an increased availability of gas supply, along with tidal interactions and a generally higher star formation efficiency in dense environments, could potentially enhance star formation activity in galaxies located in rich environments at z > 2.

Giant star-forming clumps are a prominent feature of star-forming galaxies (SFGs) and contain important clues on galaxy formation and evolution. However, the basic demographics of clumps and their host galaxies remain uncertain. Using the Hubble Space Telescope/Wide Field Camera 3 F275W images from the Ultraviolet Imaging of the Cosmic Assembly Near-infrared Deep Extragalactic Legacy Survey, we detect and analyze giant star-forming clumps in galaxies at 0.5 ≤ z ≤ 1, connecting two epochs when clumps are common (at cosmic high noon, z ∼ 2) and rare (in the local Universe). We construct a clump sample whose rest-frame 1600 Å luminosity is 3 times higher than the most luminous local H ii regions ( M _UV ≤ −16 AB). In our sample, 35% ± 3% of low-mass galaxies (log[ M _∗ / M _⊙ ] < 10) are clumpy (i.e., containing at least one off-center clump). This fraction changes to 22% ± 3% and 22% ± 4% for intermediate (10 ≤ log[ M _∗ / M _⊙ ] ≤ 10.5) and high-mass (log[ M _∗ / M _⊙ ] > 10.5) galaxies, in agreement with previous studies. When compared to similar-mass nonclumpy SFGs, low- and intermediate-mass clumpy SFGs tend to have higher star formation rates (SFRs) and bluer rest-frame U − V colors, while high-mass clumpy SFGs tend to be larger than nonclumpy SFGs. However, clumpy and nonclumpy SFGs have similar Sérsic index, indicating a similar underlying density profile. Furthermore, we investigate how the UV luminosity of star-forming regions correlates with the physical properties of host galaxies. On average, more luminous star-forming regions reside in more luminous, smaller, and/or higher specific SFR galaxies and are found closer to their hosts’ galactic centers.

We present the survey design, implementation, and outlook for COSMOS-Web, a 255 hr treasury program conducted by the James Webb Space Telescope in its first cycle of observations. COSMOS-Web is a contiguous 0.54 deg ^2 NIRCam imaging survey in four filters (F115W, F150W, F277W, and F444W) that will reach 5 σ point-source depths ranging ∼27.5–28.2 mag. In parallel, we will obtain 0.19 deg ^2 of MIRI imaging in one filter (F770W) reaching 5 σ point-source depths of ∼25.3–26.0 mag. COSMOS-Web will build on the rich heritage of multiwavelength observations and data products available in the COSMOS field. The design of COSMOS-Web is motivated by three primary science goals: (1) to discover thousands of galaxies in the Epoch of Reionization (6 ≲ z ≲ 11) and map reionization’s spatial distribution, environments, and drivers on scales sufficiently large to mitigate cosmic variance, (2) to identify hundreds of rare quiescent galaxies at z > 4 and place constraints on the formation of the universe’s most-massive galaxies ( M _⋆ > 10 ^10 M _⊙ ), and (3) directly measure the evolution of the stellar-mass-to-halo-mass relation using weak gravitational lensing out to z ∼ 2.5 and measure its variance with galaxies’ star formation histories and morphologies. In addition, we anticipate COSMOS-Web’s legacy value to reach far beyond these scientific goals, touching many other areas of astrophysics, such as the identification of the first direct collapse black hole candidates, ultracool subdwarf stars in the Galactic halo, and possibly the identification of z > 10 pair-instability supernovae. In this paper we provide an overview of the survey’s key measurements, specifications, goals, and prospects for new discovery.

High-resolution imaging of galaxies in rest-frame UV has revealed the existence of giant star-forming clumps prevalent in high-redshift galaxies. Studying these substructures provides important information about their formation and evolution and informs theoretical galaxy evolution models. We present a new method to identify clumps in galaxies’ high-resolution rest-frame UV images. Using imaging data from CANDELS and UVCANDELS, we identify star-forming clumps in an HST/F160W ≤ 25 AB mag sample of 6767 galaxies at 0.5 ≤ z ≤ 3 in four fields, GOODS-N, GOODS-S, EGS, and COSMOS. We use a low-passband filter in Fourier space to reconstruct the background image of a galaxy and detect small-scale features (clumps) on the background-subtracted image. Clumpy galaxies are defined as those having at least one off-center clump that contributes a minimum of 10% of the galaxy’s total rest-frame UV flux. We measure the fraction of clumpy galaxies ( f _clumpy ) as a function of stellar mass, redshift, and galaxy environment. Our results indicate that f _clumpy increases with redshift, reaching ∼65% at z ∼ 1.5. We also find that f _clumpy in low-mass galaxies ( $9.5\leqslant \mathrm{log}({M}_{* }/{M}_{\odot })\leqslant 10$ ) is 10% higher compared to that of their high-mass counterparts ( $\mathrm{log}({M}_{* }/{M}_{\odot })\gt 10.5$ ). Moreover, we find no evidence of significant environmental dependence of f _clumpy for galaxies at the redshift range of this study. Our results suggest that the fragmentation of gas clouds under violent disk instability remains the primary driving mechanism for clump formation, and incidents common in dense environments, such as mergers, are not the dominant processes.

We present a new method based on information theory to find the optimal number of bands required to measure the physical properties of galaxies with desired accuracy. As a proof of concept, using the recently updated COSMOS catalog (COSMOS2020), we identify the most relevant wave bands for measuring the physical properties of galaxies in a Hawaii Two-0- (H20) and UVISTA-like survey for a sample of i < 25 AB mag galaxies. We find that with the available i -band fluxes, r , u , IRAC/ ch 2, and z bands provide most of the information regarding the redshift with importance decreasing from r band to z band. We also find that for the same sample, IRAC/ ch 2, Y , r , and u bands are the most relevant bands in stellar-mass measurements with decreasing order of importance. Investigating the intercorrelation between the bands, we train a model to predict UVISTA observations in near-IR from H20-like observations. We find that magnitudes in the YJH bands can be simulated/predicted with an accuracy of 1 σ mag scatter ≲0.2 for galaxies brighter than 24 AB mag in near-IR bands. One should note that these conclusions depend on the selection criteria of the sample. For any new sample of galaxies with a different selection, these results should be remeasured. Our results suggest that in the presence of a limited number of bands, a machine-learning model trained over the population of observed galaxies with extensive spectral coverage outperforms template fitting. Such a machine-learning model maximally comprises the information acquired over available extensive surveys and breaks degeneracies in the parameter space of template fitting inevitable in the presence of a few bands.

Atacama Large Millimeter/submillimeter Array (ALMA) observations of the long-wavelength dust continuum are used to estimate the gas masses in a sample of 708 star-forming galaxies at z = 0.3−4.5. We determine the dependence of gas masses and star formation efficiencies (SFEs; SFR per unit gas mass) on redshift (z), M _* , and star formation rate (SFR) relative to the main sequence (MS). We find that 70% of the increase in SFRs of the MS is due to the increased gas masses at earlier epochs, while 30% is due to increased efficiency of star formation (SF). For galaxies above the MS this is reversed—with 70% of the increased SFR relative to the MS being due to elevated SFEs. Thus, the major evolution of star formation activity at early epochs is driven by increased gas masses, while the starburst activity taking galaxies above the MS is due to enhanced triggering of star formation (likely due to galactic merging). The interstellar gas peaks at z = 2 and dominates the stellar mass down to z = 1.2. Accretion rates needed to maintain continuity of the MS evolution reach >100 M _⊙ yr ^−1 at z > 2. The galactic gas contents are likely the driving determinant for both the rise in SF and AGN activity from z = 5 to their peak at z = 2 and subsequent fall at lower z . We suggest that for self-gravitating clouds with supersonic turbulence, cloud collisions and the filamentary structure of the clouds regulate the star formation activity.

We study the recent star formation histories (SFHs) of 575 intermediate-mass galaxies (IMGs, 10 ^9 ≤ M / M _⊙ ≤ 10 ^10 ) in COSMOS at 0.3 < z < 0.4 by comparing their H α and UV luminosities. These two measurements trace star formation rates (SFRs) on different timescales and together reveal fluctuations in recent activity. We compute L _H _α from Magellan IMACS spectroscopy, while L _UV is derived from rest-frame 2800 Å photometry. Dust corrections are applied to each band independently. We compare the deviation of L _H _α and L _UV from their respective star-forming sequences (i.e., ${\rm{\Delta }}\mathrm{log}{L}_{{\rm{H}}\alpha }$ and ${\rm{\Delta }}\mathrm{log}{L}_{\mathrm{UV}}$ ), and after accounting for observational uncertainties we find a small intrinsic scatter between the two quantities ( σ _δ ≲ 0.03 dex). This crucial observational constraint precludes strong fluctuations in the recent SFHs of IMGs: simple linear SFH models indicate that a population of IMGs would be limited to only factors of ≲2 change in SFR over 200 Myr and ≲30% on shorter timescales of 20 Myr. No single characteristic SFH for IMGs, such as an exponentially rising/falling burst, can reproduce the individual and joint distribution of ${\rm{\Delta }}\mathrm{log}{L}_{{\rm{H}}\alpha }$ and ${\rm{\Delta }}\mathrm{log}{L}_{\mathrm{UV}}$ . Instead, an ensemble of SFHs is preferred. Finally, we find that IMG SFHs predicted by recent hydrodynamic simulations, in which feedback drives rapid and strong SFR fluctuations, are inconsistent with our observations.

We explore how the fraction of quenched galaxies changes in groups of galaxies with respect to the distance to the center of the group, redshift, and stellar mass to determine the dominant process of environmental quenching in 0.2 < z < 0.8 groups. We use new UV data from the UVCANDELS project in addition to existing multiband photometry to derive new galaxy physical properties of the group galaxies from the zCOSMOS 20 k group catalog. Limiting our analysis to a complete sample of log ( M _* / M _⊙ ) > 10.56 group galaxies, we find that the probability of being quenched increases slowly with decreasing redshift, diverging from the stagnant field galaxy population. A corresponding analysis on how the probability of being quenched increases with time within groups suggests that the dominant environmental quenching process is characterized by slow (∼Gyr) timescales. We find a quenching time of approximately ${4.91}_{-1.47}^{+0.91}$ Gyr, consistent with the slow processes of strangulation and delayed-then-rapid quenching although more data are needed to confirm this result.

The diversity of the human microbiome is positively associated with human health. However, this diversity is endangered by Westernized dietary patterns that are characterized by a decreased nutrient variety. Diversity might potentially be improved by promoting dietary patterns rich in microbial strains. Various collections of bacterial cultures resulting from a century of dairy research are readily available worldwide, and could be exploited to contribute towards this end. We have conducted a functional in silico analysis of the metagenome of 24 strains, each representing one of the species in a bacterial culture collection composed of 626 sequenced strains, and compared the pathways potentially covered by this metagenome to the intestinal metagenome of four healthy, although overweight, humans. Remarkably, the pan-genome of the 24 strains covers 89% of the human gut microbiome’s annotated enzymatic reactions. Furthermore, the dairy microbial collection covers biological pathways, such as methylglyoxal degradation, sulfate reduction, γ-aminobutyric (GABA) acid degradation and salicylate degradation, which are differently covered among the four subjects and are involved in a range of cardiometabolic, intestinal, and neurological disorders. We conclude that microbial culture collections derived from dairy research have the genomic potential to complement and restore functional redundancy in human microbiomes.

Human capital is considered as one of the major factors to promote economic stability, especially in developing countries. Furthermore, one of the most important factors in developing human capital is taking the advantage of facilities and economic capabilities in the Instruction Sector. Development of financial system provides such abilities for the prospective countries. This paper studies the influence of financial development on human capital in Iran over period 1977-2010 with the application of a VAR model. The results show that the cash flow in Iran has a negative effect on human capital, which could be because of the increase in inflation and ends increase in costs of long-term investment and human capital is taken into account as long term investment. However, the facilities provided by the banking system has negative effect on human capital due to the lack of the best financial resource allocation. However, since most of university graduate students in Iran practically have adequate skills and education, they do not have enough capital to start a business. Providing financial assistance for the private sector can lead to a business in which they can use their skills and education towards promoting production.

We report the spectroscopic confirmation of a new protocluster in the COSMOS field at z ∼ 2.2, COSMOS Cluster 2.2 (CC2.2), originally identified as an overdensity of narrowband selected Hα emitting candidates. With only two masks of Keck/MOSFIRE near-IR spectroscopy in both H (∼1.47–1.81 μm) and K (∼1.92–2.40 μm) bands (∼1.5 hr each), we confirm 35 unique protocluster members with at least two emission lines detected with S/N > 3. Combined with 12 extra members from the zCOSMOS-deep spectroscopic survey (47 in total), we estimate a mean redshift and a line-of-sight velocity dispersion of zmean = 2.23224 ± 0.00101 and σlos = 645 ± 69 km s−1 for this protocluster, respectively. Assuming virialization and spherical symmetry for the system, we estimate a total mass of Mvir ∼ (1–2) ×1014M⊙ for the structure. We evaluate a number density enhancement of δg ∼ 7 for this system and we argue that the structure is likely not fully virialized at z ∼ 2.2. However, in a spherical collapse model, δg is expected to grow to a linear matter enhancement of ∼1.9 by z = 0, exceeding the collapse threshold of 1.69, and leading to a fully collapsed and virialized Coma-type structure with a total mass of Mdyn(z = 0) ∼ 9.2 × 1014M⊙ by now. This observationally efficient confirmation suggests that large narrowband emission-line galaxy surveys, when combined with ancillary photometric data, can be used to effectively trace the large-scale structure and protoclusters at a time when they are mostly dominated by star-forming galaxies. [ABSTRACT FROM AUTHOR]

We present a robust method, weighted von Mises kernel density estimation, along with boundary correction to reconstruct the underlying number density field of galaxies. We apply this method to galaxies brighter than Hubble Space Telescope AB mag in the redshift range 0.4 ≤ z ≤ 5 in the five CANDELS fields (GOODS-N, GOODS-S, EGS, UDS, and COSMOS). We then use these measurements to explore the environmental dependence of the star formation activity of galaxies. We find strong evidence of environmental quenching for massive galaxies (M ≳ 1011M⊙) out to z ∼ 3.5 such that an overdense environment hosts ≳20% more massive quiescent galaxies than an underdense region. We also find that environmental quenching efficiency grows with stellar mass and reaches ∼60% for massive galaxies at z ∼ 0.5. The environmental quenching is also more efficient than stellar mass quenching for low-mass galaxies (M ≲ 1010M⊙) at low and intermediate redshifts (z ≲ 1.2). Our findings concur thoroughly with the “overconsumption” quenching model where the termination of cool gas accretion (cosmological starvation) happens in an overdense environment and the galaxy starts to consume its remaining gas reservoir in depletion time. The depletion time depends on the stellar mass and could explain the evolution of environmental quenching efficiency with stellar mass. [ABSTRACT FROM AUTHOR]