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Your search keyword '"plastid thioredoxins"' showing total 3 results
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3 results on '"plastid thioredoxins"'

1. Thioredoxins m regulate plastid glucose-6-phosphate dehydrogenase activity in Arabidopsis roots under salt stress.

Author

Née, Guillaume, Fuzheng Wang, Châtel-Innocenti, Gilles, Mhamdi, Amna, Juranville, Eugénie, Vanacker, Hélène, Noctor, Graham, and Issakidis-Bourguet, Emmanuelle

Subjects
GLUCOSE-6-phosphate dehydrogenase, ARABIDOPSIS, NICOTINAMIDE adenine dinucleotide phosphate, SALT, DATA protection, DEHYDROGENASES
Abstract

Plants contain several NADPH-producing enzymes including glucose-6-phosphate dehydrogenases (G6PDH) with different sub-cellular localizations. The activity of plastidial G6PDHs is redox-regulated by thioredoxins (TRX). Although specific TRXs are known to regulate chloroplastic isoforms of G6PDH, little information is available for plastidic isoforms found in heterotrophic organs or tissues. Here, we investigated TRX regulation of the two G6PDH plastidic isoforms of Arabidopsis roots during exposure to a mild salt stress. We report that in vitro m-type TRXs are the most efficient regulators of the G6PDH2 and G6PDH3 mainly found in Arabidopsis roots. While expression of the corresponding G6PD and plastidic TRX genes was marginally affected by salt, it impaired root growth of several of the corresponding mutant lines. Using an in situ assay for G6PDH, G6PDH2 was found to be the major contributor to saltinduced increases in activity, while data from ROS assays further provide in vivo evidence that TRX m acts in redox regulation during salt stress. Taken together, our data suggest that regulation of plastid G6PDH activity by TRX m may be an important player regulating NADPH production in Arabidopsis roots undergoing salt stress. [ABSTRACT FROM AUTHOR]

Published
2023
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2. Thioredoxins m regulate plastid glucose-6-phosphate dehydrogenase activity in Arabidopsis roots under salt stress

Author

Guillaume Née, Fuzheng Wang, Gilles Châtel-Innocenti, Amna Mhamdi, Eugénie Juranville, Hélène Vanacker, Graham Noctor, and Emmanuelle Issakidis-Bourguet

Subjects
glucose-6-phosphate dehydrogenase, redox regulation, Arabidopsis root, salt stress, plastid thioredoxins, Plant culture, SB1-1110
Abstract

Plants contain several NADPH-producing enzymes including glucose-6-phosphate dehydrogenases (G6PDH) with different sub-cellular localizations. The activity of plastidial G6PDHs is redox-regulated by thioredoxins (TRX). Although specific TRXs are known to regulate chloroplastic isoforms of G6PDH, little information is available for plastidic isoforms found in heterotrophic organs or tissues. Here, we investigated TRX regulation of the two G6PDH plastidic isoforms of Arabidopsis roots during exposure to a mild salt stress. We report that in vitro m-type TRXs are the most efficient regulators of the G6PDH2 and G6PDH3 mainly found in Arabidopsis roots. While expression of the corresponding G6PD and plastidic TRX genes was marginally affected by salt, it impaired root growth of several of the corresponding mutant lines. Using an in situ assay for G6PDH, G6PDH2 was found to be the major contributor to salt-induced increases in activity, while data from ROS assays further provide in vivo evidence that TRX m acts in redox regulation during salt stress. Taken together, our data suggest that regulation of plastid G6PDH activity by TRX m may be an important player regulating NADPH production in Arabidopsis roots undergoing salt stress.

Published
2023
Full Text
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3. Papel de las tiorredoxinas plastidiales en la regulación del metabolismo del cloroplasto

Author

Ancín Rípodas, María, Farrán Blanch, Inmaculada, Universidad Pública de Navarra. Departamento de Agronomía, Biotecnología y Alimentación, and Nafarroako Unibertsitate Publikoa. Agronomia, Bioteknologia eta Elikadura Saila

Subjects
Metabolismo, Plastid thioredoxins, Cloroplasto, Tiorredoxinas plastidiales, Chloroplast metabolism
Abstract

Carbon (C) and nitrogen (N) are two essential nutrients required for plant growth and development. The cellular metabolism of both elements is tightly coordinated and subjected to a complex regulation. In this regard, the redox status operates as a major integrator of cellular metabolism, involving post-translational modifications of target enzymes by thiol-disulfide exchanges exerted by thioredoxins (Trxs). In plant chloroplasts two Trx systems function in parallel: (i) the ferredoxin (Fd)-Trx system that depends on photosynthetically reduced Fd to supply electrons to the Fd-Trx reductase, which in turn reduces Trxs; (ii) the NADPH dependent Trx reductase type C (NTRC) that contains both a Trx-reductase and a Trx domain in a single polypeptide. NTRC is reduced by NADPH, which in addition to the light reactions, is also produced in the oxidative pentose phosphate pathway in darkness. This PhD thesis has been focused on the regulatory role of plastid Trx f, m and NTRC in chloroplast metabolism. El carbono (C) y el nitrógeno (N) son dos nutrientes esenciales para el crecimiento y desarrollo de la planta. Los metabolismos de estos dos elementos se encuentran estrechamente coordinados y sometidos a una compleja regulación dentro de la célula. En este sentido, el estado redox cellular constituye uno de los principales integradores del metabolismo, incluyendo modificaciones post-traduccionales sobre enzimas diana mediante intercambios ditiol/disulfuro llevados a cabo por las tiorredoxinas (Trxs). En los cloroplastos actúan dos sistemas Trx en paralelo: (i) el sistema ferredoxina (Fd)-Trx en el que la Fd reducida durante la fotosíntesis transmite los electrones a la Fd-Trx reductasa, que a su vez reduce a las Trxs; (ii) el sistema formado por la Trx reductasa tipo C dependiente de NADPH (NTRC), que contiene en un mismo polipéptido un dominio Trx reductasa y un dominio Trx. NTRC es reducida tanto por el NADPH proveniente de las reacciones dependientes de la luz, como por el generado en la ruta oxidativa de las pentosas fosfato en oscuridad. Esta tesis doctoral se ha centrado en el papel regulador ejercido por las Trxs plastidiales f, m y NTRC en el metabolismo del cloroplasto. La realización de esta tesis doctoral sido posible gracias al disfrute de una ayuda para la Formación del Profesorado Universitario (FPU) con referencia FPU13/01675, y a la ayuda de Movilidad para Estancias Breves para beneficiarios de este subprograma (EST15/00101), ambas concedidas por el Ministerio de Ciencia, Innovación y Universidades. La financiación para la realización de esta tesis doctoral ha sido posible gracias a los proyectos “Ingeniería genética en cultivos energéticos alternativos para su utilización en la producción de bioetanol” del Ministerio de Ciencia e Innovación (AGL2010-15107) y “Papel de las tiorredoxinas plastidiales en la regulación del metabolismo del almidón en plantas” de la Fundación Caja Navarra. Programa de Doctorado en Biotecnología (RD 99/2011) Bioteknologiako Doktoretza Programa (ED 99/2011)

Published
2019

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