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Antibody activity against HSA and KLH was present in all five immunoglobulin classes of BALB/c mice. Bordetella pertussis vaccine was more effective in stimulating antibody production against HSA than against KLH and IgG1 and IgG2b antibody were affected more than the antibody present in the other immunoglobulin classes. The results are discussed in relation to the differing thymic dependence of these two antigens. [ABSTRACT FROM AUTHOR]

Studies were continued on the mode of action of a methanol-insoluble moiety (methanol-extraction residue) of attenuated tubercle bacilli which enhances the immune response. We analysed the effects of treatment with this material on the primary and secondary immune responses of mice to two antigens, sheep red blood cell and T2 phage. Pretreatment with methanol-extraction residue enhanced the primary response to sheep red blood cells, accelerating the formation of both 19S and 7S plaque-forming cells and of circulating haemagglutinins, and prolonging high levels of 19S and 7S plaque-forming cells. Methanol-extraction residue delayed the secondary response to sheep red blood cells when administered between primary and secondary immunization. Methanol- extraction residue administration subsequent to immunization did not affect an ongoing primary response to this antigen. Administration of methanol-extraction residue before primary immunization with T2 phage, or between primary and secondary immunizations, enhanced both the primary and secondary circulating antibody responses to this antigen. The findings are discussed in relation to possible sites of action of methanol-extraction residue. [ABSTRACT FROM AUTHOR]

At least two different types of plaque-forming cells (PFC) participating in the Immune response of mice to sheep red blood cells (SRBC) are identified. This is demonstrated by (a) two PFC-peaks appearing at different times in response to the same low dose of antigen; (b) the morphology of the PFC in each of the two peaks is different; (c) the PFC in each peak respond differently to the suppressive effect of anti-S RBC serum. [ABSTRACT FROM AUTHOR]