Search

A radioimmunoassay for the quantitative determination of the immobilization antigens of Paramoecium is described. The use of this technique in the identification of closely related antigens is discussed in terms of antigen 'profiles', derived from precipitin tests with several antisera. [ABSTRACT FROM AUTHOR]

Antisera prepared in rabbits against frog oviducal homogenate produce a minimum of six to nine precipitation bands in agar-gel diffusion plates. Individual bands were isolated from several plates and injected into rabbits to produce antibodies specific to the antigen represented in the precipitate. Bands formed with antigen excess or antibody excess were effective in eliciting an immune response against the antigen. Antibodies produced against the γ-globulin portion of the precipitate were detected in the serum of one-half the rabbits injected. Rats injected with the above isolated precipitates failed to produce antisera specific to individual rabbit antibodies. Agar-gel diffusion and immunoelectrophoresis showed that isolated precipitin bands may be used for the production of specific antisera against a single antigen in a complex mixture. [ABSTRACT FROM AUTHOR]

Antigens prepared from Fasciola spp. precipitated in agar with antisera obtained from mice or rabbits infected with Fasciola hepatica, and with antisera from cattle infected with F. gigantica, or from rabbits immunized with preparations of adult F. hepatica. In the latter there were at least thirty-eight different antibodies, reacting with both protein and species specific non-protein antigens. The reactions of the antisera from infected animals were less complex. Antisera from infected cattle or mice gave little or no reaction with protein antigens, whereas antisera from infected rabbits reacted predominantly with one of the protein antigens. Certain non-protein components of the somata or metabolic products of the flukes also reacted with antisera from infected animals. These components were not species specific, and appeared to be haptens as they did not react with antisera from rabbits immunized with killed fluke antigen. [ABSTRACT FROM AUTHOR]

Rabbit antisera against mouse cells have been absorbed with normal mouse serum and used to detect mouse cell-bound antigens. Mouse ascites tumor cells and their products have mainly been used as source of antigens but those described here are normal mouse components. Seven antigens have been found by agar diffusion precipitin methods and data are given to show that each is a separate molecular entity. Information is provided for the identification of each component. [ABSTRACT FROM AUTHOR]

Quantitative precipitin tests have been carried out with fowl antisera to BSA and BGG using isotopically labeled antigens. The peculiarities of fowl precipitating antisera raise analytical difficulties which are most easily overcome by the use of labeled reagents. The results have confirmed the importance of the NaCl concentration of the medium in determining the amount of antibody precipitated. An apparent anomaly in the AbN/AgN ratio curves may be explainable in terms of the properties of 'heavy' and 'light' antibodies in fowl sera. A rapid method for the analysis of fowl sera for precipitins is described. [ABSTRACT FROM AUTHOR]

By immunizing rabbits with bacteria agglutinated by corresponding immune rabbit sera group iso-precipitins were obtained. Using the iso-precipitins thus obtained the rabbit sera could be divided into two serum groups: D (a+) and D (a−); for grouping the gel diffusion technique was applied. The serum groups are heritable; probably group D (a+) mothers can transfer Da protein non-genetically. The substance thus obtained is then gradually eliminated from the bloodstream of the young animal. Da antigen has γglobulin electrophoretic mobility as shown by means of immunoelectrophoresis. The incidence of group D(a+) in 92 unrelated rabbits was 27·2 per cent. The authors discuss the relationship between iso-precipitins and anti-bodies and the practical significance of rabbit serum groups analogous to human Gm serum groups. [ABSTRACT FROM AUTHOR]