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Precipitin lines were observed in double diffusion tests between mouse gut tissue homogenates and serum from the same animal. Other tissues failed to produce this result. The line-forming components of the tissue are lipoidal while serum reactants are free of lipid. Purified γ-globulin, IgG, does not react with tissue constituents. An IgM component can be identified as one reactant. Lipo- polysaccharides extracted from E. coli 014, Forssman antigen and Kidd-Friede- wald antigen could not be demonstrated as reactants. It appears that certain tissue components which may be lipoproteins, and lipid free serum components, one of which is IgM, react in a way which resembles immunological interactions but which arc believed not to be so. [ABSTRACT FROM AUTHOR]

The studies reported here indicate that tanned red cells coated with a mixture of chromatographically purified ovalbumin and conalbumin give agglutination titres which are directly related to the amount of antibody (as meas- ured by quantitative precipitation) present against either of these antigens in mixed sera. With whole (unabsorbed) mixed sera, these coated cells record the titre of the major antibody present, whether this is anti-ovalbumin or anti-conalbumin. There was no evidence of greater sensitivity to anti-conalbumin. A trace of ovalbumin in a preparation of conalbumin used to coat cells is fully sufficient to enable them to react with any anti-ovalbumin in a serum. Twice recrystallized ovalbumin still contains some conalbumin. Sera raised against it may contain unexpectedly high levels of anti-conalbumin and this could cause confusion in tanned red cell tests. [ABSTRACT FROM AUTHOR]

Antisera to specific proteins in herpes simplex infected cells were produced by immunization of rabbits with infected rabbit kidney cells. These antisera were highly virus specific and produced up to twelve lines in immunodiffusion tests against infected cell extracts. Acrylamide electrophoresis and immunoelectrophoresis revealed up to ten virus specific proteins of varying size. [ABSTRACT FROM AUTHOR]

The article presents a list of abbreviations related to immunology. Some of the abbreviation given are for antibody, antigen, acid-citrate-dextrose, autoimmune complement fixation, antilymphocyte globulin, bis-diazotized benzidine, biological false positive reaction, bovine gammaglobulin, bovine serum albumin, Bence-Jones, buffered saline solution, carboxymethyl, cerebrospinal fluid, diethylaminoethyl, deoxyribonucleic acid, dinitrochlorbenzenc, ethylenediamine tetracetic acid, erythrocyte sedimentation rate, fluorescein to protein, gonococeal complement fixation test, graft versus host, intraperitoneal, intravenous, keyhole limpet haemocyanin, long acting thyroid stimulator, lymphocytic choriomeningitis, lupus erythematosus and so on.