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Slowly sedimenting components with a sedimentation velocity of about 7S and reacting with specific antisera against IgM were found in six pathological human sera. Four of these came from patients with different forms of reactive macroglobulinaemia of infectious origin. Similar components were produced from purified IgM preparations by bacterial action, and to a lesser extent by spontaneous splitting at room temperature. The conditions for such a splitting were, however, absent for most of the sera investigated in this study. The possibility that slowly sedimenting IgM-like components are artefacts, is discussed. [ABSTRACT FROM AUTHOR]

Antisera to specific proteins in herpes simplex infected cells were produced by immunization of rabbits with infected rabbit kidney cells. These antisera were highly virus specific and produced up to twelve lines in immunodiffusion tests against infected cell extracts. Acrylamide electrophoresis and immunoelectrophoresis revealed up to ten virus specific proteins of varying size. [ABSTRACT FROM AUTHOR]

Canine precipitating and non-precipitating antisera against bovine serum albumin where studied by quantitative isotope precipitin and coprecipitation techniques. Canine precipitating antisera yielded curves of incomplete precipitation of antigen in antibody excess. Non-precipitating canine antibody can inhibit precipitation of antigen by rabbit precipitating antibody under certain conditions. Canine precipitating antibody contains a &gamma-globulin fraction which results in complete precipitation of antigen in antibody excess. It is suggested that canine non-precipitating antibody is a faster moving globulin which is present in canine precipitating antisera and the combined effect of canine non precipitating and precipitating antibodies results in the characteristic types of canine precipitin curve. [ABSTRACT FROM AUTHOR]