1. Ammonium Sulfate and Water Dialysis Fractionation of Chicken Serum as Studied by Paper Electrophoresis
- Author
-
Irwin W. Sherman and Robert W. Hull
- Subjects
Plasmodium lophurae ,Ammonium sulfate ,Chromatography ,Chemistry ,General Medicine ,Fractionation ,Serology ,Electrophoresis ,chemistry.chemical_compound ,Immune system ,Biochemistry ,Parasite hosting ,Animal Science and Zoology ,Whole blood - Abstract
PREVIOUS investigations employing the chemical fractionation of serum have presumably isolated immune factors for the avian malaria parasite, Plasmodium lophurae (Becker et al., 1951; Trager and McGhee, 1950; and Truong, 1957). Electorophoretic analysis has also identified serum alterations which appear to be linked to an immune response (Sherman and Hull, 1960). One of the logical adjuncts to the study of the serological changes described by paper electrophoresis is the isolation, purification and characterization of these entities, and correlation of the chemical fractions with the electrophoretic species. Studies of the chemical fractions by means of paper electrophoresis were initiated in the hope of better understanding the relationships between these fractionation procedures. MATERIALS AND METHODS For the chemical fractionation, whole blood was withdrawn from the jugular vein of mature, uninfected Hyline chickens. These samples were incubated at 39°C. in lusteroid centrifuge tubes for one hour to assure maximum clot formation. They were …
- Published
- 1961