1. Sui Sarcomi Derivati Dal Rivestimento Sinoviale Delle Guaine Tendinee, Delle Articolazioni, Delle Borse Mucose
- Author
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Yang Yan, Jian-Hua Huang, Jun Luo, Jiang Geng, Yang Shao, Feng-qiang Yang, Jian-Ping Che, Jun-hua Zheng, and Chang-cheng Guo
- Subjects
Cancer Research ,medicine.medical_specialty ,Valproic Acid ,medicine.diagnostic_test ,General Medicine ,Biology ,Cell cycle ,medicine.disease ,Cell counting ,030218 nuclear medicine & medical imaging ,Flow cytometry ,03 medical and health sciences ,Cyclin E1 ,0302 clinical medicine ,Endocrinology ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,Internal medicine ,Carcinoma ,medicine ,Cancer research ,lipids (amino acids, peptides, and proteins) ,IC50 ,medicine.drug - Abstract
Objective: To investigate the effects of VPA (valproate) on proliferation, cell cycle distribution and apoptosis of human kidney carcinoma ACHN cells and the possible underlying mechanisms. Methods: The effect of VPA on the proliferation of ACHN cells was examined by CCK-8 (cell counting kit-8) assay. Flow cytometry was used to analyze the cell cycle distribution and apoptosis of ACHN cells exposed to VPA. The mRNA expressions of cyclin E1, P21WAF1, Bcl-2 and Bax were detected by real-time fluorescence quantitative-PCR. Results: Incubation with various concentrations of VPA for 48 h resulted in a significant inhibition of proliferation of ACHN cells with an IC50 (50% inhibitory concentration) value of 4.21 mmol/L. After treatment with VPA, the cell cycle was arrested obviously at G0/G1 phase and the apoptotic rate was significantly increased as compared with the control group. After treatment with 4 mmol/L VPA for 48 h, the levels of P21WAF1 and Bax mRNAs were both significantly increased, and at the same time, the levels of cyclin E1 and Bcl-2 mRNAs were obviously decreased. Conclusion: VPA can inhibit the proliferation of kidney carcinoma ACHN cells by inducing cell-cycle arrest and apoptosis. DOI:10.3781/j.issn.1000-7431.2013.03.004
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- 1947