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The fluorescent antibody technique first described by CooNs, CREECH and J o ~ s (1961) has been widely used for the detection of antigen and antibody in numerous systems including infectious diseases where it has found its widest and most important use from a public health standpoint. BOZFMAN and ELISBERG (1963 and 1967) applied the technique to the serological diagnosis of scrub typhus and their procedures are now being used in laboratories throughout the world. The technique described by them is one of the most reliable serological tests known; however, it requires collection of a volume of whole blood which is not always possible in epidemiological work or in small mammals. Other workers have encountered similar problems with other diseases and ANDERSON, Sta)trN and WILLIAMS (1961) reported on a method that used dried whole smears on filter paper in the fluorescent antibody test for the diagnosis of schistosomiasis. More recently, BAILEY, CUNNINGHAM and KIM~ER (1967) reported on the same technique adapted for a study of trypanosomiasis in Africa which Walton and Arjona reported on its adaption for use in the indirect fluorescent antibody test for toxoplasmosis. This paper reports on the combination of these two techniques for the detection and titration of antibodies against the causative agent of scrub typhns--R, tsutsugamushi.