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A simple method for the iodination of proteins of the aqueous humour with radioactive iodine 131I is described. After iodination of the proteins with radioactive iodine the aqueous humour is analysed by electrophoresis on paper strips saturated with calf-serum in veronal buffer of pH 8.5 with ionic strength of 0.07 μ at a potential drop of 3 V cm−1. The time of electrophoresis is 18 hr. The electrophoreograms are dried and washed three times with a solution of acetic acid in dilute ethylalcohol, dried again and evaluated by direct measurement of the radioactivity of the 131I ohemically bound to proteins of the aqueous humour or by photometric measurement of the autoradiograms obtained by contact exposure of the eleotrophoreograms on X-ray film. The method is very sensitive; it is therefore sufficient to use 10 to 20 μl of aqueous humour for the iodination. Ten to 20 μ of the iodinated aqueous humour, i.e. 4 to 8 μl of the original aqueous humour with a protein content of 2 to 5 μg are applied to the paper. The results of the eleotrophoretic analysis of human, rabbit and bovine aqueous humour are in good agreement with the results of analogous analyses carried out by other methods.

A review of literature on the ocular toxicity of the anti-tuberculosis drug Ethambutol Hydrochloride. Ethambutol Hydrochloride is currently extensively used in tuberculosis therapy, so this review should be of interest to those who are concerned with patients under chemotherapy for tuberculosis.