1. Elevated expression of serine/threonine phosphatase type 5 correlates with malignant proliferation in human osteosarcoma
- Author
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Kun Han, Zan Shen, Shuchen Lin, Daliu Min, Zhihua Gan, and Haiyan Hu
- Subjects
musculoskeletal diseases ,0301 basic medicine ,medicine.medical_treatment ,Endogeny ,Biology ,Resting Phase, Cell Cycle ,General Biochemistry, Genetics and Molecular Biology ,Targeted therapy ,Serine ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Phosphoprotein Phosphatases ,medicine ,Humans ,Gene Silencing ,RNA, Small Interfering ,Cell Proliferation ,Osteosarcoma ,Gene knockdown ,Cell growth ,Nuclear Proteins ,Cell Cycle Checkpoints ,Cell cycle ,medicine.disease ,Molecular biology ,Up-Regulation ,030104 developmental biology ,Cell culture ,030220 oncology & carcinogenesis - Abstract
Osteosarcoma is the most common primary malignant bone tumor in adolescents and young adults. However, the involvement of serine/threonine phosphatase type 5 (PP5) in osteosarcoma remains unclear. The aim of this study was to evaluate the functional role of PP5 in osteosarcoma cells. Firstly, we found that PP5 is widely expressed in several human osteosarcoma cell lines. Then we used lentivirus-delivered siRNA to silence PP5 expression in Saos-2 and U2OS cell lines. Knockdown of endogenous PP5 expression by shRNA-expressing lentivirus significantly decreased the viability and proliferation of the osteosarcoma cells. Moreover, FACS analysis showed that knockdown of PP5 expression induced a significant arrest in the G0/G1 phase of the cell cycle, which was associated with the inhibition of cell proliferation. Therefore, knockdown of PP5 is likely to provide a novel alternative to targeted therapy of osteosarcoma and deserves further investigation.
- Published
- 1970