1. Alterations of Platelet Aggregation in the Chandler Tube: I. Turbidimetric Studies
- Author
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Brian Anderson, Genevieve C. Holtz, and Richard B. Davis
- Subjects
Blood Platelets ,Male ,Continuous measurement ,Erythrocytes ,Time Factors ,Epinephrine ,Rotation ,Platelet aggregation ,Optical density ,chemistry.chemical_compound ,Platelet Adhesiveness ,Methods ,medicine ,Animals ,Humans ,Thrombus ,Blood Coagulation ,Factor VIII ,Adenine Nucleotides ,Venoms ,Thrombosis ,General Medicine ,medicine.disease ,Blood proteins ,Adenosine diphosphate ,Biochemistry ,chemistry ,Factor XII ,Coagulation system ,Catecholamine ,Biophysics ,Female ,Collagen ,Glass ,Rabbits ,sense organs ,Stearic Acids ,Densitometry ,medicine.drug - Abstract
Platelet aggregation in the Chandler tube was studied by continuous measurement of optical density during aggregation. Normal mean values for the duration of aggregation varied from 3.4 to 6.0 min. [rabbit platelet-rich plasma (PRP)] and 3.1 to 5.8 min. (human PRP). Mean thrombus formation time (TFT) varied from 17.1 to 20.9 min. (rabbit PRP) and from 13.5 to 16.4 min. (human PRP). Activation of Hageman factor shortened TFT and duration of aggregation, and minimized optical density changes during aggregation. Factor VIII and erythrocytes increased optical density changes during aggregation. The observations suggest that experimental and clinical states associated with an increased tendency to thrombosis may correlate with characteristic changes in optical density during aggregation in the Chandler system. Altered aggregation characteristics result from changes in plasma proteins, adenosine diphosphate release, catecholamine concentration, and the blood coagulation system.
- Published
- 1970
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