1. Nature of intercellular matrix of reorganized steroidogenic cells from juvenile chicken testes grown in vitro
- Author
-
G.C. Budd and H.H. Lee
- Subjects
Male ,Cell ,Morphogenesis ,Biology ,Cell junction ,Extracellular matrix ,Lanthanum ,Culture Techniques ,Testis ,Methods ,medicine ,Animals ,Microscopy, Phase-Contrast ,Trypsin ,Cells, Cultured ,Cell Aggregation ,Staining and Labeling ,Histocytochemistry ,Cell Biology ,In vitro ,Cell aggregation ,Cell biology ,Microscopy, Electron ,Intercellular Junctions ,medicine.anatomical_structure ,Cytochemistry ,Steroids ,Chickens ,Intracellular - Abstract
Steroidogenic cells reorganize into discrete colonies surrounded by fibroblastic cells when juvenile chicken testes cells are dissociated and cultivated in monolayers. Electron microscopic observations in combination with cytochemistry indicate the existence of electron opaque material in the intercellular spaces between the steroidogenic cells only. In the light microscope, the cell periphery of steroidogenic cells shows a positive colour reaction with Alcian blue when the cells are observed individually but a negative reaction when the cells are in groups. Results indicate that either there may be a change of the chemical nature of the intercellular material, or there may be two distinct substances reflecting two synthetic processes during cellular reaggregation and reorganization of steroidogenic cells in vitro.
- Published
- 1971