1. Studies in the biochemistry of skin V. The particulate origin of adenylate kinase in the skin of neonatal rats
- Author
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Theodore Rosett, Itsuro Matsuo, Keith W. Brown, Thomas F. McDonald, Dorothy B. Smith, and Ann Bailey
- Subjects
Male ,Sucrose ,Density gradient ,Acid Phosphatase ,Biophysics ,Ficoll ,Adenylate kinase ,Biology ,Biochemistry ,chemistry.chemical_compound ,Centrifugation, Density Gradient ,Animals ,heterocyclic compounds ,Adenylate kinase activity ,Molecular Biology ,Glucuronidase ,Skin ,chemistry.chemical_classification ,Neonatal rat ,Phosphotransferases ,Acid phosphatase ,Molecular biology ,Rats ,Microscopy, Electron ,Enzyme ,Animals, Newborn ,chemistry ,biology.protein ,Female ,Lysosomes - Abstract
Evidence is presented to show that in the skin of the neonatal rat, the enzyme adenylate kinase is associated with particles. We prepared a lysosomal fraction from neonatal rat skin and then further fractionated this material by the use of a density gradient which varied from 0–16% Ficoll and 0.88–1.4 M sucrose. Three bands were isolated which had acid phosphatase, β-glucuronidase and adenylate kinase activity. Each of these fractions was then resubjected to an expanded density gradient corresponding to the range in which it had previously sedimented. When equilibrium had been reached, the particles still exhibited acid phosphatase, β-glucuronidase and adenylate kinase activity. Repeatedly washed particles retained adenylate kinase activity.
- Published
- 1970
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