1. A novel silver-sulfoethyl cellulose column for purification of ethynyl steroids from biological fluids
- Author
-
E.D. Pellizzari, Margaret Twine, Jun Liu, and Clarence Cook
- Subjects
Chromatography, Gas ,Silver ,medicine.medical_treatment ,Biophysics ,Ethyl acetate ,Ethinyl Estradiol ,Tritium ,Biochemistry ,Mass Spectrometry ,Steroid ,Ethynodiol Diacetate ,chemistry.chemical_compound ,Dogs ,Column chromatography ,medicine ,Animals ,Organic chemistry ,Carbon Radioisotopes ,Dehydration ,Cellulose ,Molecular Biology ,Chromatography ,Chemistry ,Mestranol ,Cell Biology ,Chromatography, Ion Exchange ,medicine.disease ,Norethynodrel ,Chromatography, Thin Layer ,Methanol ,Norethindrone ,Diethyl ether - Abstract
A method for purification of ethynyl steroids from biological fluids has been developed using silver-sulfoethyl cellulose column chromatography. Ethynyl steroids were applied in methanol, and the firm binding allowed the columns to be washed with methanol, ethyl acetate, or diethyl ether to remove endogenous materials. Nonethynylated steroids did not bind to silver-sulfoethyl cellulose. Release of ethynylated steroids was achieved with a saturated NaCl/methanol solution. Dehydration or de-ethynylation of ethynyl estradiol, ethynodiol diacetate, and ethynodiol was not observed. The utility of this technique for purification of ethynyl steroid metabolites from the urine of a beagle metabolizing norethynodrel was demonstrated.
- Published
- 1973