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In order to study the uptake and metabolism of PPi by bone, 32PPi was added to the medium surrounding explanted mouse calvaria maintained in organ culture. Most of the PPi was hydrolysed during incubation, but there was a measurable entry of intact PPi into bone. When 32Pi was added to the medium, synthesis of PPi and organic phosphates from Pi was observed in bone. There was no detectable passage of PPi from bone into the medium. These results are discussed in terms of two models of pyrophosphate hydrolysis and exchange. Some quantitative estimates about the fate of PPi in bone were made.