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Antimicrobial activity of brilliant green dye in Trypticase soy broth (BBL) is reduced and ultimately destroyed by prolonged autoclaving at 121 C. Loss of antimicrobial activity is accompanied by decolorization of the dye. This is consistent with other evidence that antimicrobial activity of brilliant green resides in the colored dye ion. The dye is not decolorized when heated in distilled water or peptone, but is decolorized by heating in glucose, glycine, or sodium dodecyl sulfate, showing that decolorization results from reaction with components of the medium. To ensure optimal results, it is recommended that bacteriological media be sterilized by heat prior to addition of brilliant green dye.

DEFICIENT lacrimal secretion occurs much more commonly in adults than in children. Alacrima as a congenital defect is distinctly unusual. Sjögren1 found only 14 cases in the literature up to 1952. The only case of unilateral congenital alacrima was reported in 1884 by Morton.2 The case of unilateral lacrimal insufficiency described below is presented because of the infrequency of the condition. REPORT OF A CASE CLINICAL HISTORY. —A 10-year-old Negro boy was first seen in July 1965 with the complaint of pain and photophobia involving the right eye. This eye was said to have an abnormal appearance beginning a few weeks after birth. Tears had never been seen in the right eye after either psychic or painful stimuli. The left eye responded in a normal fashion. There was no family history of a similar condition.At the time of initial examination (Fig 1), the lids were normal. The