1. The purification and properties of hen oviduct form B DNA-dependent RNA polymerase
- Author
-
Ronald F. Cox and Michael Houghton
- Subjects
Time Factors ,Transcription, Genetic ,DNA, Single-Stranded ,Oviducts ,Peptides, Cyclic ,Chromatography, DEAE-Cellulose ,chemistry.chemical_compound ,In vivo ,RNA polymerase ,Genetics ,Centrifugation, Density Gradient ,Animals ,Ultrasonics ,Polymerase ,Oligopeptide ,Nuclease ,Deoxyribonucleases ,biology ,Basidiomycota ,DNA ,DNA-Directed RNA Polymerases ,Articles ,Mycotoxins ,Chromatography, Ion Exchange ,Molecular biology ,In vitro ,Kinetics ,Biochemistry ,chemistry ,biology.protein ,Oviduct ,Electrophoresis, Polyacrylamide Gel ,Female ,Chickens ,Oligopeptides - Abstract
Hen oviduct form B DNA-dependent RNA polymerase has been extensively purified and its properties analysed. It seems likely to consist of a mixture of two forms of the type observed in tissues from other species. Furthermore using S1 nuclease to digest single-stranded DNA, we show that although form B can transcribe double-stranded DNA template it has a very strong preference for single-stranded regions. Also the rate of elongation on native DNA in vitro was measured and is an order of magnitude slower than that reported to be operative in vivo.
- Published
- 1974