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In rats given a single injection (500 μg) in the hind footpads of heterologous (water-soluble) renal glycoprotein and Freund's incomplete adjuvant, proliferative glomerulonephritis with immunofluorescent `mesangial pattern' was induced. Immunopathological and morphological changes of this group were very similar to those of rats which received a single injection of homologous and water-soluble renal glycoprotein. The nephritogenic renal glycoprotein does not show species specificity. [ABSTRACT FROM AUTHOR]

Rats given a single injection in the hind footpads of homologous, water-soluble, organ glycoprotein and Freund's incomplete adjuvant developed proliferative glomerulonephritis. Among the glycoproteins obtained from various rat organs, the nephritogenic activity (on a dry weight basis) of the active glycoprotein prepared from lung was a little lower than that from renal cortex, and was followed by those from aorta, heart, liver, spleen and muscle in that order. This nephritogenic glycoprotein is present also in renal medulla, and also in renal pyramids (inner medulla) which contain neither glomeruli nor proximal convoluted tubules. The activity of nephritogenic glycoproteins prepared from various rat organs is nearly parallel with both the hexose content and the yield of the cathodal part of the hexose peak in zone electrophoresis. Nephritogenic glycoproteins obtained from various rat organs share a structural component isolated from GBM. However, the immunofluorescence pattern in the glomerulonephritis induced by a single injection of each of these glycoproteins was 'mesangial' rather than 'linear'. [ABSTRACT FROM AUTHOR]