1. Purification of 125I-labelled lysine-vasopressin by affinity chromatography on sepharose-bound neurophysins.
- Author
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Fressinaud PH, Corvol P, Frenoy JP, and Menard J
- Subjects
- Animals, Biochemistry methods, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Iodine chemistry, Kidney metabolism, Lysine chemistry, Pituitary Gland metabolism, Radioimmunoassay methods, Swine, Chromatography, Affinity instrumentation, Chromatography, Affinity methods, Iodine Radioisotopes chemistry, Lypressin chemistry, Neurophysins chemistry, Sepharose chemistry
- Abstract
Purification of 125I-labelled lysine-vasopressin has been achieved by affinity chromatography on a Sepharose 4 B conjugate of porcine neurophysins. This affinity absorbent did not retain halogenated hormone, while native lysine-vasopressin was bound on the solid support. The specific activity of purified iodinated lysine-vasopressin was 1700-1800 Ci/g, corresponding to one iodine atom per mole. By comparison with an unpurified tracer, a five times increase in the minimum detectable dose was obtained in the vasopressin radioimmunoassay.
- Published
- 1973
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