1. Metabolism of Tryptophan in Isolated Perfused Rat Liver1,2
- Author
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Ng, C.Y., Hagino, Y., Swan, P.B., and Henderson, L.M.
- Abstract
The fate of tryptophan in the isolated perfused rat liver was investigated. It was shown that the liver removed the L-isomer more rapidly from the perfusate than the D-isomer. The 10 g liver was capable of a removal rate of 35 mg/hour when 42 mg of DL-tryptophan was given initially, followed by infusion at the rate of 42 mg/hour. Carbon-14 from DL-tryptophan-2-14C appeared in CO2much more rapidly and to a greater extent within 3 hours than did that from DL-tryptophan-7a-14C. In contrast to whole-animal studies, it was shown that overloading doses of acetoacetate were labeled by DL-tryptophan-5- or 7a-14C. The labeling pattern in acetoacetate was similar to that observed when 14C-fatty acids are the substrate. Acetoacetate overloading suppressed the production of radioactive carbon dioxide from DL-tryptophan-7a-14C.
- Published
- 1969
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