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CULTURE of dissociated lymphoid cells in vitro enables the description of various characteristics of an immune response as manifested under defined conditions—but not the time course of evolution. Normal lymphoid cell cultures are usually short term, those of spleen or lymph node cells from common laboratory animals rarely surviving longer than 12–14 d. Lymphoblastoid or plasmocytoma lines, established in continuous cultures originated from cancerous or Epstein–Barr virus-bearing tissues, seem to have lost their capacity for responding to specific antigens. We have shown1,2that calf lymphoid cells can be maintained in more prolonged cultures (up to three months) without losing their immunocompetence. We report results obtained using dissociated calf lymph node cell cultures surviving for more than 2 yr.