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The economic management of lignocellulosic biomass from semi-natural grasslands is now a challenge across Europe. The abandonment of mowing these grasslands leads to the gradual degradation of these ecosystems. This study investigates how chemical and biological factors affect the suitability of biomass from abandoned grasslands for biogas production. We sampled 30 mown and 30 abandoned grassland sites in the Sudetes Mountains (Poland and Czechia). The cover contribution of short herbs was found to be significantly higher in mown grasslands (p < 0.001), while that of tall herbs was more prevalent in abandoned grasslands (p < 0.01). The specific biogas yield (SBY, NL kg -1 volatile solids) is negatively affected by an increased percentage of herbs in the biomass of mown and abandoned grasslands. This is due to the inhibitory effect of herbs on biodegradation, the increase in lignin content and the decrease in cellulose. This study highlights the importance of individual plant species in assessing grassland biomass for area biogas yield (ABY, m 3 ha -1 ) and provides new insights into a field that has not yet been extensively investigated. In mown grasslands, ABY was most positively correlated with grass species (Arrhenatherum elatius, Trisetum flavescens and Festuca pratensis). In abandoned grasslands, the ABY was most correlated with herbaceous species (Galium aparine, Urtica dioica and Chaerophyllum aromaticum) and grasses (A. elatius and Elymus repens). Mown grasslands had significantly higher species richness (p < 0.001) compared to abandoned grasslands, but the number of species sampled did not correlate with SBY and ABY. This study contributes to the development of a sustainable bio-economy by highlighting the need for efficient use of grassland biomass. This approach helps protect semi-natural ecosystems and facilitates sustainable management of renewable resources., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

This comprehensive study addresses the urgent global challenges of climate change and environmental degradation by focusing on the Ecological Footprint (EF). Unlike previous studies, it introduces a novel approach incorporating spatial spillover, temporal effects, and common shocks in panel data analysis. The spatial spillover effect highlights the influence of trade, pollution havens, and competition between neighboring countries on EF. The temporal effects emphasize the significance of historical production patterns and export strategies in shaping the current EF. The study also considers the impact of exogenous common shocks, such as international agreements and global events, on EF. Utilizing a dynamic spatial panel data model with common shocks, the research examines 40 European countries from 1992 to 2020, revealing the significant impact of biocapacity, energy consumption, industrialization, and globalization on EF. Findings indicate that spatial spillover effects contribute to EF transfer, emphasizing the need for collaborative global efforts. The study sheds light on the interconnectedness of environmental impacts and underscores the importance of considering both weak and strong forms of cross-sectional dependence in achieving accurate estimations. The research enriches our understanding of EF determinants and provides nuanced insights for policymakers striving to develop effective strategies for sustainable resource management and environmental conservation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

European manufacturing firms have to cope with the new regulations that advocate a greener and more sustainable future with less emissions and at the same time enhance or at least maintain their productivity levels. A unique dataset is constructed by combining information on different firms' pollutants with their financial information during the 2011-2017 period. A non-radial directional distance function analysis is adopted with desirable and undesirable outputs to estimate environmental productivity growth and its components, which addresses the problem of heterogeneity. A regulatory impact indicator that provides information about the loss of outputs resulting from new policies is also computed. Finally, the impact of environmental regulations on productivity growth is explored using a panel vector autoregressive method. Our findings showcase different average values of productivity for each pollutant group. Moreover, results indicate that increasing the index of regulations by 1%, increases environmental productivity by 0.24% and 0.44% for heavy metals and greenhouse gases groups, respectively. Finally, results support the "weak" Porter Hypothesis, which attests that welldesigned environmental regulations can exert a positive effect on environmental innovation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Introduced into Europe from North America 150 years ago alongside its native crayfish hosts, the invasive pathogen Aphanomyces astaci is considered one of the main causes of European crayfish population decline. For the past two centuries, this oomycete pathogen has been extensively studied, with the more recent efforts focused on containing and monitoring its spread across the continent. However, after the recent introduction of new strains, the newly-discovered diversity of A. astaci in North America and several years of coevolution with its European host, a new assessment of the traits linked to the pathogen's virulence is much needed. To fill this gap, we investigated the presence of phenotypic patterns (i.e., in vitro growth and sporulation rates) possibly associated with the pathogen's virulence (i.e., induced mortality in crayfish) in a collection of 14 A. astaci strains isolated both in North America and in Europe. The results highlighted a high variability in virulence, growth rate and motile spore production among the different strains, while the total-sporulation rate was more similar across strains. Surprisingly, growth and sporulation rates were not significantly correlated with virulence. Furthermore, none of the analysed parameters, including virulence, was significantly different among the major A. astaci haplogroups. These results indicate that each strain is defined by a characteristic combination of pathogenic features, specifically assembled for the environment and host faced by each strain. Thus, canonical mitochondrial markers, often used to infer the pathogen's virulence, are not accurate tools to deduce the phenotype of A. astaci strains. As the diversity of A. astaci strains in Europe is bound to increase due to translocations of new carrier crayfish species from North America, there is an urgent need to deepen our understanding of A. astaci's virulence variability and its ability to adapt to new hosts and environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

We introduce an analytical methodological framework that links knowledge generation efficiency with economic efficiency and the corresponding environmental impact for 199 European Regions during 2000-2018, using a benchmarking approach and especially a chain network DEA technique. A clear trade-off between knowledge generation efficiency and productive performance emerges. European regions which exhibit high innovation efficiency enjoy higher overall performance compared to their counterparts. In a second stage, we investigate the convergence patterns of the examined regions with respect to all the three facets of the estimated efficiency where the coexistence of multi-type convergence clubs is revealed., Competing Interests: Declaration of competing interest The authors declare that they have not any competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Achieving global restoration targets poses challenges including the need for long-term research and effective monitoring of success, fostering collaborations across diverse fields and actors, ensuring the availability of suitable reference ecosystems, and securing sustained funding. Yet, these conditions are often lacking, limiting the effectiveness of restoration. We provide an overview of ecological restoration practices in the pan-European region of the Long-term Ecological Research Network (eLTER) and demonstrate the importance of eLTER and its potential contributions to support the implementation of the EU Nature Restoration Law. We developed an online questionnaire to collect information on eLTER restoration experts and restoration projects details including the use of eLTER contributions (e.g. infrastructure, data and knowledge), between November 2021 and March 2022. We identified 62 restoration experts and 42 restoration projects from 18 countries. Our results show that eLTER restoration expertise covers most of the European habitats, diverse degradation states and restoration techniques. Most restoration projects (78%) involved long-term monitoring exceeding the average project lifespan, which has proven necessary to achieve restoration success. No common protocol was used for monitoring and evaluation or cost-benefit estimates, but respondents reported effective projects, mostly financed from national funds, and benefits in five ecosystem services on average covered per project. Key eLTER contributions included providing reference ecosystems, biotic and abiotic background data, and interdisciplinary discussion or stakeholder management. Ecological restoration is time intensive and requires long-term research and monitoring standardization to fully understand the restoration process and to ensure comparability across ecosystems. The eLTER network can help address these challenges providing added-value contributions through its infrastructure, long-term datasets, diversity of expertise and strategies that can help identify best restoration practices and support the EU Nature Restoration Law. Finally, additional and long-term funding from the EU and the private sector is needed to achieve global larger-scale restoration targets., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Mountain forests play an essential role in protecting people and infrastructure from natural hazards. However, forests are currently experiencing an increasing rate of natural disturbances (including windthrows, bark beetle outbreaks and forest fires) that may jeopardize their capacity to provide this ecosystem service in the future. Here, we mapped the risk to forests' protective service across the European Alps by integrating the risk components of hazard (in this case, the probability of a disturbance occurring), exposure (the proportion of forests that protect people or infrastructure), and vulnerability (the probability that the forests lose their protective structure after a disturbance). We combined satellite-based data on forest disturbances from 1986 to 2020 with data on key forest structural characteristics (cover and height) from spaceborne lidar (GEDI), and used ensemble models to predict disturbance probabilities and post-disturbance forest structure based on topographic and climatic predictors. Wind and bark beetles are dominant natural disturbance agents in the Alps, with a mean annual probability of occurrence of 0.05%, while forest fires were less likely (mean annual probability <0.01%), except in the south-western Alps. After a disturbance, over 40% of forests maintained their protective structure, highlighting the important role of residual living or dead trees. Within 30 years after wind and bark beetle disturbance, 61% of forests were likely to either maintain or recover their protective structure. Vulnerability to fires was higher, with 51% of forest still lacking sufficient protective structure 30 years after fire. Fire vulnerability was especially pronounced at dry sites, which also had a high fire hazard. Combining hazard and vulnerability with the exposure of protective forests we identified 186 Alpine municipalities with a high risk to protective forests due to wind and bark beetles, and 117 with a high fire risk. Mapping the disturbance risk to ecosystem services can help identify priority areas for increasing preparedness and managing forests towards lower susceptibility under an intensifying disturbance regime., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Porcine respiratory coronavirus (PRCV) was initially detected in Europe, and later in the United States of America (US), in the 1980s. In this study we obtained and compared PRCV sequences from Europe and the US, and investigated how these are related to transmissible gastroenteritis virus (TGEV) sequences. The whole genome sequences of Danish (1/90-DK), Italian (PRCV15087/12 III NPTV Parma), and Belgian PRCV (91V44) strains are presented. These sequences were aligned with nine other PRCV sequences from Europe and the US, and 43 TGEV sequences. Following alignment of the PRCV sequences, it was apparent that multiple amino acid variations in the structural proteins were distinct between the European and US strains. The alignments were used to build phylogenetic trees to infer the evolutionary relationships between the strains. In these trees, the European PRCV strains clustered as a separate group, whereas the US strains of PRCV all clustered with TGEVs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Sewage sludge management is crucial for water utilities to move towards a circular valorisation of resources. The current literature focuses mainly on the technological aspects of sludge management strategies. However, the current discussion of these strategies does not consider possible pressures arising from the utilities' civil society stakeholders and from policymakers. To fill this gap, this paper develops a conceptual framework, based on the current literature, that identifies the utility's key decisions on sludge management strategies (valorisation route, overperformance and vertical integration), and links them to possible pressures arising from civil society and existing regulations. Subsequently, the study validates the framework through a multiple explanatory case study, investigating the empirical relevance of such pressures in six water utilities across Europe. The influence of citizens and municipalities is found to be crucial in the choice of sludge valorisation routes. Economic instruments, command and control instruments and, new to the literature, regulatory uncertainty are found to be key policy features influencing utilities' decisions on sludge management. The paper provides a first-of-its-kind investigation that highlights the mechanisms through which policymakers and civil society stakeholders shape utilities' sewage sludge management strategies. The results complement and extend existing theoretical knowledge on the role of institutional pressures in the implementation of sustainable environmental systems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Studies to date have predominantly focused on countries' socioeconomic conditions (e.g., income inequality) to explain cross-national differences in socioeconomic inequalities in adolescent health (behaviours). However, the potential explanatory role of sociocultural contexts at country-level remains underexamined. This study examined whether the country-level sociocultural context and changes thereof were associated with adolescent socioeconomic inequalities in dietary behaviours. International comparative data of 344,352 adolescents living in 21 countries participating in 2002, 2006, 2010 and 2014 waves of the Health Behaviour in School-aged Children (HBSC) survey were combined with aggregated levels of openness-to-change from the European Social Survey (ESS). Four dietary behaviours (i.e., fruit, vegetable, sweets and soft drink consumption) and two measures of socioeconomic status (SES) on the individual level (i.e., family affluence scale [FAS] and occupational social class [OSC]) were studied. Multilevel logistic regression analyses returned contrasting results for the two SES measures used. In countries with higher levels of openness-to-change, smaller FAS inequalities in daily fruit, sweets and soft drink consumption were observed, but no such inequalities were found for vegetable consumption. Conversely, in these countries, larger OSC inequalities in soft drink consumption were found. Country-specific changes in openness-to-change over time were not associated with the magnitude of adolescent dietary inequalities. Findings underscore the importance of including country-level sociocultural contexts to improve the understanding of cross-national differences in socioeconomic inequalities in adolescents' diets. Future studies, spanning a longer timeframe, are required to examine whether such associations exist within countries over time since our timeframe might have been too small to capture these long-term trends., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Soil microbial biomass and activity strongly depend on land use, vegetation cover, climate, and soil physicochemical properties. In most cases, this dependence was assessed by one-to-one correlations while by employing network analysis, information about network robustness and the balance between stochasticity and determinism controlling connectivity, was revealed. In this study, we further elaborated on the hypothesis of Smith et al. (2021) that cropland soils depended more on climate variables and therefore are more vulnerable to climate change. We used the same dataset with that of Smith et al. (2021) that contains seasonal microbial, climate and soil variables collected from 881 soil points representing the main land uses in Europe: forests, grassland, cropland. We examined complete (both direct and indirect relationships) and incomplete networks (only direct relationships) and recorded higher robustness in the former. Partial Least Square results showed that on average more than 45% of microbial attributes' variability was predicted by climate and habitat drivers denoting medium to strong effect of habitat filtering. Network architecture slightly affected by season or land use type; it followed the core/periphery structure with positive and negative interactions and no hub nodes. Microbial attributes (biomass, activity and their ratio) mostly belong to core block together with Soil Organic Carbon (SOC), while climate and soil variables to periphery block with the exception of cropland networks, denoting the higher dependence between microbial and climate variables in these latter. All complete networks appeared robust except for cropland and forest in summer, a finding that disagrees with our initial hypothesis about cropland. Networks' connectivity was controlled stronger by stochasticity in forest than in croplands. The lack of human interventions in forest soils increase habitat homogeneity enhancing the influence of stochastic agents such as microbial unlimited dispersal and/or stochastic extinction. The increased stochasticity implies the necessity for proactive management actions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The global decline of freshwater mussels and their crucial ecological services highlight the need to understand their phylogeny, phylogeography and patterns of genetic diversity to guide conservation efforts. Such knowledge is urgently needed for Unio crassus, a highly imperilled species originally widespread throughout Europe and southwest Asia. Recent studies have resurrected several species from synonymy based on mitochondrial data, revealing U. crassus to be a complex of cryptic species. To address long-standing taxonomic uncertainties hindering effective conservation, we integrate morphometric, phylogenetic, and phylogeographic analyses to examine species diversity within the U. crassus complex across its entire range. Phylogenetic analyses were performed using cytochrome c oxidase subunit I (815 specimens from 182 populations) and, for selected specimens, whole mitogenome sequences and Anchored Hybrid Enrichment (AHE) data on ∼ 600 nuclear loci. Mito-nuclear discordance was detected, consistent with mitochondrial DNA gene flow between some species during the Pliocene and Pleistocene. Fossil-calibrated phylogenies based on AHE data support a Mediterranean origin for the U. crassus complex in the Early Miocene. The results of our integrative approach support 12 species in the group: the previously recognised Unio bruguierianus, Unio carneus, Unio crassus, Unio damascensis, Unio ionicus, Unio sesirmensis, and Unio tumidiformis, and the reinstatement of five nominal taxa: Unio desectusstat. rev., Unio gontieriistat. rev., Unio mardinensisstat. rev., Unio nanusstat. rev., and Unio vicariusstat. rev. Morphometric analyses of shell contours reveal important morphospace overlaps among these species, highlighting cryptic, but geographically structured, diversity. The distribution, taxonomy, phylogeography, and conservation of each species are succinctly described., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

Hydro-morphological threats impact the natural physical characteristics of river ecosystems, such as flow regimes, sediment transport, and channel morphology. These negative effects can occur at multiple scales, ranging from local microhabitats to geographic regions. Understanding these interactions can be useful for an integrated conservation approach and is needed for effective freshwater management. The aim of the study was to elucidate the combined effects of hydro-morphological threats on macroinvertebrates at three spatial scales: macroscale, including whole catchments, mesoscale (hydro-morphological changes in individual river sections) and the microscale, describing the microhabitat conditions of European rivers. The diversity and trophic structure of 1120 local macroinvertebrate communities in 28 catchments of various hydro-morphological disturbance levels, ranging from 0 to 2400 m asl, were analyzed. The response of macroinvertebrates to the main disturbance gradient differed between mountain and lowland communities. Random forest analysis indicated that the most important predictor of the ecological, diversity, and trophic indices was described by flow rate reduction. Generalized additive mixed models showed that decreased flow combined with river incision explained most of the variation in macroinvertebrate indices. Our results emphasize that based on multi-spatial scale analysis, hydro-morphological threats are very important factors in invertebrates biodiversity loss. Thus, to implement effective river management, we should pay more attention to the combined effects of geomorphological threats., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

At least 87% of angiosperm species require animal vectors for their reproduction, while more than two-thirds of major global food crops depend on zoogamous pollination. Pollinator insects are a wide variety of organisms that require diverse biotic and abiotic resources. Many factors have contributed to a serious decrease in the abundance of populations and diversity of pollinator species over the years. This decline is alarming, and the European Union has taken several actions aimed at counteracting it by issuing new conservation policies and standardizing the actions of member countries. In 2019, the European Green Deal was presented, aiming to restore 100% of Europe's degraded land by 2050 through financial and legislative instruments. Moreover, the Common Agricultural Policies have entailed greening measures for the conservation of habitats and beneficial species for more than 10 years. The new CAP (CAP 23-27) reinforces conservation objectives through strategic plans based on eco-schemes defined at the national level by the member countries, and some states have specifically defined eco-schemes for pollinator conservation. Here, we review the framework of EU policies, directives, and regulations, which include measures aimed at protecting pollinators in agricultural, urban, and peri-urban environments. Moreover, we reviewed the literature reporting experimental works on the environmental amelioration for pollinators, particularly those where CAP measures were implemented and evaluated, as well as studies conducted in urban areas. Among CAP measures, several experimental works have considered the sowing and management of entomophilous plants and reported results important for environmental ameliorations. Some urban, peri-urban and wasteland areas have been reported to host a considerable number of pollinators, especially wild bees, and despite the lack of specific directives, their potential to contribute to pollinator conservation could be enhanced through targeted actions, as highlighted by some studies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

European legislation stated that electric vehicles' sale must increase to 35% of circulating vehicles by 2030, and concern is associated to the batteries' supply chain. This review aims at analysing the impacts (about material flows and CO 2 eq emissions) of Lithium-Ion Batteries' (LIBs) recycling at full-scale in Europe in 2030 on the European LIBs' supply-chain. Literature review provided the recycling technologies' (e.g., pyro- and hydrometallurgy) efficiencies, and an inventory of existing LIBs' production and recycling plants in Europe. European production plants exhibit production capacity adequate for the expected 2030 needs. The key critical issues associated to recycling regard pre-treatments and the high costs and environmental impacts of metallurgical processes. Then, according to different LIBs' composition and market shares in 2020, and assuming a 10-year battery lifetime, the Material Flow Analysis (MFA) of the metals embodied in End of Life (EoL) LIBs forecasted in Europe in 2030 was modelled, and the related CO 2 eq emissions calculated. In 2030 the European LIBs' recycling structure is expected to receive 664 t of Al, 530 t of Co, 1308 t of Cu, 219 t of Fe, 175 t of Li, 287 t of Mn and 486 t of Ni. Of these, 99% Al, 86% Co, 96% Cu, 88% Mn and 98% Ni will be potentially recovered by pyrometallurgy, and 71% Al, 92% Co, 92% Fe, 96% Li, 88 % Mn and 90% Ni by hydrometallurgy. However, even if the recycling efficiencies of the technologies applied at full-scale are high, the treatment capacity of European recycling plants could supply as recycled metals only 2%-wt of the materials required for European LIBs' production in 2030 (specifically 278 t of Al, 468 t of Co, 531 t of Cu, 114 t of Fe, 95 t of Li, 250 t of Mn and 428 t of Ni). Nevertheless, including recycled metals in the production of new LIBs could cut up 28% of CO 2 eq emissions, compared to the use of virgin raw materials, and support the European batteries' value chain., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

The assessment of risk posed by climate change in coastal cities encompasses multiple climate-related hazards. Sea-level rise, coastal flooding and coastal erosion are important hazards, but they are not the only ones. The varying availability and quality of data across cities hinders the ability to conduct holistic and standardized multi-hazard assessments. Indeed, there are far fewer studies on multiple hazards than on single hazards. Also, the comparability of existing methodologies becomes challenging, making it difficult to establish a cohesive understanding of the overall vulnerability and resilience of coastal cities. The use of indicators allows for a standardized and systematic evaluation of baseline hazards across different cities. The methodology developed in this work establishes a framework to assess a wide variety of climate-related hazards across diverse coastal cities, including sea-level rise, coastal flooding, coastal erosion, heavy rainfall, land flooding, droughts, extreme temperatures, heatwaves, cold spells, strong winds and landslides. Indicators are produced and results are compared and mapped for ten European coastal cities. The indicators are meticulously designed to be applicable across different geographical contexts in Europe. In this manner, the proposed approach allows interventions to be prioritized based on the severity and urgency of the specific risks faced by each city., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Small watercourses are essential contributors to catchment water quality, but they continue to suffer degradation across Europe. A results-based agri-environment scheme, aimed at improving watercourse quality in Ireland, developed a rapid drainage assessment to identify point source risks. The assessment uses a scoring system based on visual indicators of nutrient and sediment risk, linking the outcomes to farmer payments. To understand how this novel drainage risk assessment relates to instream watercourse quality, we used three macroinvertebrate-based biotic indices (Q-value, Small Stream Impact Score and Proportion of Sediment Sensitive Invertebrates). Macroinvertebrate kick-sampling and physiochemical analysis were completed in May and July 2021 for 12 'At Risk' and 12 'Not at Risk' small watercourses as identified by the results-based scheme. Results show that the scheme's drainage risk assessment can identify point source risks but we found it does not directly reflect local instream quality as assessed by the biotic indices. Unexpectedly, the biotic indices showed watercourse degradation in 58% of the upstream (control) sampling points, indicating impacts not captured by the drainage risk assessment. Small watercourses displayed high heterogeneity, with significant species turnover between the sampling months. The Small Stream Impact Score was less influenced by temporal change than the Q-value index. There was a significant relationship between instream watercourse quality and sedimentation, as quantified by the Proportion of Sediment Sensitive Invertebrates. Including a measurement of instream sedimentation in the drainage assessments would improve the identification of risks and management. These results show that by linking farmer payments to the drainage risk assessments results-based payment schemes could positively contribute to improving catchment scale watercourse quality, but further work is required to capture wider sources of freshwater impacts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The Mediterranean basin is one of the most vulnerable regions worldwide due to its population density, the concentration of economic activities along the coasts and borderline climatic balance. It is identified as one of the most critical erosion hotspots in Europe, mainly due to the degradation of coastal areas, overexploitation and unsustainable practices affecting beach tourism, agriculture and fishing. The region is also affected by other phenomena such as storms and floods, which are exacerbated by climate change. To mitigate and adapt to these environmental and climatic changes, Nature based Solutions (NbSs) are considered a promising step-forward. However, despite their global recognition in both research and policy, few scientific papers and documents on the state of NbSs implementation for coastal risk management in the Mediterranean exist. This paper aims to provide an understanding of the status of NbS adoption for coastal risk management in the Mediterranean through a literature review. Out of 162 scientific papers and documents, only 23 were found to be relevant to the study. Through the definition and support of an innovative matrix-based approach, the analysis of the state of adoption of NbSs have been performed. Despite the limited information on the state of the adoption of NbSs for coastal risk management in the Mediterranean due to the low numbers of scientific research and documents available, some key considerations have been revealed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Focusing on the European context, this paper re-examines the relationship between the ESG and financial performance and whether CSR attitude moderates this relationship. A panel data set with all the listed companies in STOXX Europe 600, covering the period 2012-2022 was built, with company data being sourced from the Refinitiv Eikon platform for a total of 6600 firm-year observations. Six measures of financial performance and three regression frameworks were considered for analysis. Overall, inconsistent patterns of effect are identified across ESG predictors and financial performance measures. The presence of a CSR committee was found to negatively moderate the effect of ESG score on ROA only, whereas an external auditor to the CSR report to negatively moderate it with share price. Future research should consider replicating the proposed re-examination framework in other data settings and legislative and accounting contexts to strengthen the current evidence base and therefore deepen our understanding of the mechanisms underlying the investigated relationships., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author. Published by Elsevier Ltd.. All rights reserved.)

Deadwood is a key old-growth element in European forests and a cornerstone of biodiversity conservation practices in the region, recognized as an important indicator of sustainable forest management. Despite its importance as a legacy element for biodiversity, uncertainties remain on the drivers of deadwood potentials, its spatial distribution in European forests and how it may change in the future due to management and climate change. To fill this gap, we combined a comprehensive deadwood dataset to fit a machine learning and a Bayesian hurdle-lognormal model against multiple environmental and socio-economic predictors. We deployed the models on the gridded predictors to forecast changes in deadwood volumes in Europe under alternative climate (RCP4.5 and RCP8.5) and management scenarios (biodiversity-oriented and production-oriented strategies). Our results show deadwood hotspots in montane forests of central Europe and unmanaged forests in Scandinavia. Future climate conditions may reduce deadwood potentials up to 13% under a mid-century climate, with regional losses amounting to up to 22% in Southern Europe. Nevertheless, changes in management towards more biodiversity-oriented strategies, including an increase in the share of mixed forests and extended rotation lengths, may mitigate this loss to a 4% reduction in deadwood potentials. We conclude that adaptive management can promote deadwood under changing environmental conditions and thereby support habitat maintenance and forest multifunctionality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The latest report on the state of nature in Europe (2013-2018) shows that biodiversity is declining at an alarming rate, with most protected species and habitats in poor condition. Despite an increasing volume of collected biodiversity information, urgent action is needed to integrate biodiversity data and knowledge to improve conservation efforts. We conducted a study in Catalonia (NE Spain), where we collected management measures implemented between 2013 and 2018, including allocation, budget, pressures aimed, and habitats/species potentially benefiting. We integrated information on pressures and habitats/species with the measures to identify non-spatial management gaps. Then, we integrated the spatially explicit information to determine the spatial management gap, identifying geographical areas where species/habitats are under pressure without registered measures. We demonstrated the importance of integrating existing information. Our findings revealed that resources were often not distributed adequately across species/habitats, with biases towards certain taxa being a common issue. The non-spatial management gap analysis identified taxonomic groups, especially plants and mollusks with the wider management gaps. We also identified threatened areas, especially in the northeast of the region with the larger spatial management gaps. These results could guide priority objectives to optimize conservation efforts. Integrating different information sources provided a broader view of the challenges that conservation science is facing nowadays. Our study offers a path toward bending the curve of biodiversity loss by providing an integrative framework that could optimize the use of the available information and help narrow the knowing-doing gap. In the context of the EU, this example demonstrates how information can be used to promote some environmental policy instruments, such as the Prioritized Action Frameworks (PAFs). Additionally, our findings highlight the importance of supporting decision-making with systematic assessments to identify deficiencies in the conservation process, reduce the loss of critical ecosystems and species, and avoid biases among taxa., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Urban forests as nature-based solutions (UF-NbS) play a crucial role for promoting the health and well-being of urban populations. Despite increasing recognition of their importance, knowledge gaps remain regarding UF-NbS implementation, particularly with respect to their performance, their perception, and on decision-making and governance practices for their implementation and management. Grounded in a project-as-practice approach and based on sixteen UF-NbS case histories, this paper seeks to gain insights into UF-NbS project actualities by an identification of characteristic trends as a function of a projects' scope and purpose, or challenge-orientation, respectively. A particular emphasis is on project structure and coordination, on the scope and depth of participation and stakeholder involvement, and on funding mechanisms. Identified trends reveal centrally coordinated, single-responsible public authorities as the most common project structure, but with certain feature contents, i.e., regeneration and reclamation, as being associated with more decentralised forms of governance. It is concluded that irrespective of challenge-orientation, comparatively broad participation appears to be realised within the UF-NbS framework, however, the depth of participation differs considerably. With respect to funding, a strong reliance on public capital is asserted for the UF-NbS context, however, various forms of private contributions appear reasonably common across the assessed case histories. Recommendations for policy-making and avenues of future research are identified, including the validation of identified trends, the fostering of collaboration, the dispersal of decision-making authority, the encouragement for implementing non-tokenistic modes of participation in UF-NbS governance, and the promotion of potentially under-utilised funds., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The ongoing invasion of Russia of Ukraine and energy crises have sparked concern about economic and geopolitical stability all over the world. In Europe, the war has destabilized and endangered the energy cooperation and transition between European countries within and outside of the EU. This emergency has shown once more the importance of energy resilience policies to offset the vulnerability of energy systems and energy insecurity at the national and regional levels. Consilience has been reached on the necessity of enhancing EU energy security as an adaptation strategy. This work contributes to the existing scholarship on renewable energy transition and citizens' perception, focusing on European Union member states. Key characteristics of the renewable energy transition in the EU prior to the energy crisis and the war in Ukraine are examined. To this end, we analyze selected economic, environmental, social, policy and political variables on energy sorting from the Eurobarometer studying European citizens' perceptions. The exercise makes use of spatially-clustered regression to explore spatial heterogeneity and to elicit determinant information on specific regional groups. We learn that southern Europeans attribute less importance to energy infrastructure to facilitate the renewable energy transition and repute the EU solidity not a requirement for energy security access. Conversely, northern European citizens tend not to associate the responsibility of the EU in guiding competitiveness and policy toward green energy sources transformation. Robustness tests confirm our hypothesis. Regardless of regional differences, the EU energy and ecological transition will thrive with industrial and political cohesion. This process will pass through increased trust in institutions and dedicated energy policy action which will smooth the risks and disruptions coming from current and future shocks., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Geospatial fire behaviour and fire hazard simulators, fire effects models and smoke emission software commonly use standard fuel models in order to simplify data collection and the inclusion of complex fuel scenarios. These fuel models are often mapped using remotely sensed data. However, given the great complexity of fuelbeds, with properties that vary widely in both time and space, the use of these standard fuel models can greatly limit accurate fuel mapping. This affects fuel hazard assessment, fuel reduction treatment plans, fire management decision-making and evaluation of the environmental impact of wildfire. In this study, we developed unique customized fire behaviour fuel models for shrub and bracken communities, by using k-medoids clustering analysis based on both fuel structural characteristics and potential fire behaviour. We used an original database of 722 destructive sample plots in nine different shrub and bracken communities covering the entire distribution area in Galicia (NW Spain), one of the regions in Europe most affected by forest fires. Measurements of cover, height and fuel fractions loads differentiated by size and vegetative state (live or dead) were used to estimate the potential rate of fire spread with five different models including fireline intensity, heat per unit area and the flame length for each sampling site and considering extreme environmental conditions. The optimal number of clusters was established by combining practical knowledge about the shrubland communities under study and their associated fire behaviour, with maximization of the mean value of the silhouette variable and minimization of the within-cluster sum of squares. The structural characteristics of the medoids derived from the analysis were associated with each of the proposed customized fuel models. Finally, a simple dichotomous classification based only on shrub height was developed to enable construction of spatially explicit fuel model maps based on remotely sensed data. Thus, the methodology applied allows generation of a more realistic representation of fuel distribution in the landscape, based on fuel structure measurements of natural regional ecosystems rather than on the use of standard models. We believe that the proposed methodology is generally applicable to communities composed of other shrub and fern species in different biogeographical regions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The microbiome influences a variety of host-environment interactions, and there is mounting evidence of its significant role in biological invasions. During invasion, shifts in microbial diversity and function can occur due to both changing characteristics of the novel environment and physiological condition of the host. The signal crayfish (Pacifastacus leniusculus) is one of the most successful crayfish invaders in Europe. During range expansion, its populations often exhibit differences in many traits along the invasion range, including sex-composition, size-structure and aggressiveness, but to date it was not studied whether crayfish traits can also drive changes in the host microbiome. Thus, we used 16S rRNA gene amplicon sequencing to examine the effects of host-related traits, namely total length (TL), body condition index (FCF), hepatosomatic index (HSI) and sex on the microbial diversity of the signal crayfish. We examined both external (exoskeletal) and internal (intestinal, hepatopancreatic, hemolymph) microbiomes of 110 signal crayfish individuals from four sites along its invasion range in the Korana River, Croatia. While sex did not exhibit a significant effect on the microbial diversity in any of the examined tissues, exoskeletal, intestinal and hemolymph microbial diversity significantly decreased with increasing crayfish size. Additionally, significant effects of signal crayfish condition (FCF, HSI) on microbial diversity were recorded in the hepatopancreas, a main energy storage organ in crayfish that supports reproduction and growth and also regulates immune response. Our findings provide a baseline for evaluating the contribution of microbiome to an invader's overall health, fitness and subsequent invasion success., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Ornamental trade has become an important introduction pathway of non-native aquatic species worldwide. Correspondingly, there has been an alarming increase in the number of established crayfish of aquarium origin in Europe over the previous decade. The oomycete Aphanomyces astaci, the pathogen causing crayfish plague responsible for serious declines of European crayfish populations, is dispersed with introduced North American crayfish. The role of ornamental taxa in introducing and spreading different genotypes of this pathogen in open waters remains unclear. We investigated the distribution, prevalence, and diversity of A. astaci in Budapest, Hungary, which became a hotspot of aquarium crayfish introductions. Their establishment in this area was facilitated by locally abundant thermal waters. We screened for A. astaci in six host taxa from 18 sites sampled between 2018 and 2021: five cambarids (Cambarellus patzcuarensis, Faxonius limosus, Procambarus alleni, P. clarkii, P. virginalis) and one native astacid (Pontastacus leptodactylus). The pathogen was confirmed at five sampled sites in four host taxa: P. virginalis, P. clarkii, F. limosus, and for the first time in European open waters also in P. alleni. Genotyping was successful only in individuals from two different brooks where multiple host species coexisted but revealed unexpected patterns. Mitochondrial B-haplogroup of A. astaci, previously usually reported from Pacifastacus leniusculus or infected European species, was detected in P. virginalis at both sites, and in both F. limosus and P. virginalis sampled from a thermally stable tributary of Barát brook in 2018. In contrast, A-haplogroup of A. astaci was detected in coexisting F. limosus, P. virginalis and P. clarkii sampled in the same watercourse just a few hundred meters downstream in 2020. Additional genotyping methods indicated that a previously unknown A. astaci strain was associated with the latter haplogroup. One P. virginalis individual from 2020 was apparently co-infected by strains representing both mitochondrial haplogroups. The results indicated multiple sources of A. astaci in Budapest, likely directly associated with the introduction of ornamental species, interspecific transmission of this pathogen among ornamental hosts, and potential for a quick spatial or temporal turnover of dominant A. astaci strains at a certain locality. This highlights that in regions with high richness of potential A. astaci hosts, host taxon/pathogen genotype combinations become unpredictable, which might prevent reliable genotyping of pathogen sources in local crayfish mass mortalities., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

This study is about the role of consumers' personal values (Self-enhancement, Openness to change, Self-transcendence, and Conservation) in consumers' interest towards meat alternatives. In addition, the underlying role of two social motives, status and group affiliation are analysed. A conceptual model with hypotheses was developed and validated, and the hypotheses were tested through PLS-SEM with data from four European countries (Finland, the UK, Germany, and Sweden, total N = 3600). The results show that self-focused personal values (Self-enhancement and Openness to change) are not associated with consumers' interest towards meat alternatives. The case is different with other-focused values. Self-transcendence had a positive connection to interest while Conservation had a negative relationship. Finally, the data suggest an underlying role of social motive status between Self-enhancement and interest and the same for group affiliation between Self-transcendence and Conservation and interest. Based on the results, strategies to support meat alternative adoption such as value activation through priming, cognition and emotion-driven marketing are proposed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)