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As a recognized safe starter, lactic acid bacteria can improve the quality characteristics of fermented sausages and ensure the safety characteristics of sausages to a certain extent. The purpose of this study was to analyze the effects of Lactobacillus helveticus IMAUJBH1 on lipid profiles and volatile flavor compounds of Inner Mongolia mutton fermented sausage by lipidomics and GC-MS. The results showed that a total of 2024 lipid molecules were identified, and 1135 differential lipids were screened by multivariate analysis (P < 0.05, VIP>1). Pathway topology analysis showed that glycerophospholipid metabolism, glyceride metabolism and sphingolipid metabolism pathways were the three most active pathways. Furthermore, 21 key differential lipid molecules and 5 key volatile flavor compounds were selected as characteristic substances for correlation analysis. The results of correlation study indicated that the addition of Lactobacillus helveticus IMAUJBH1 would further affect the flavor of fermented mutton sausage by affecting the relative expression of lipid molecules LPC (17:0), LPC (22:6), LPC (18:1), LPC (18:3), LPC (22:4), Cer (d18:1/16:0), and Cer (d18: 1/22:0). The results provide a scientific theoretical basis for the application of IMAUJBH1 in mutton fermented sausage, and further promote the development of mutton processing industry in Inner Mongolia. • Lipidomics was used to evaluate the lipid metabolism of fermented sausages. • 21 Key differential lipid molecules were identified as characteristic substances. • 5 key volatile flavor substances (OAV>1) were used as characteristic substances. • IMAUJBH1 can reduce the content of related aldehydes and esters. [ABSTRACT FROM AUTHOR]

Pompano fishes have been widely farmed worldwide. As a representative commercial marine species of the Carangidae family, the golden pompano (Trachinotus blochii) has gained significant popularity in China and worldwide. However, because of rapid growth and high-density aquaculture, the golden pompano has become seriously threatened by various diseases. Cell lines are the most cost-effective resource for in vitro studies and are widely used for physiological and pathological research owing to their accessibility and convenience. In this study, we established a novel immortal cell line, GPF (Golden pompano fin cells). GPF has been passaged over 69 generations for 10 months. The morphology, adhesion and extension processes of GPF were evaluated using light and electron microscopy. GPF cells were passaged every 3 days with L-15 containing 20 % fetal bovine serum (FBS) at 1:3. The optimum conditions for GPF growth were 28 °C and a 20 % FBS concentration. DNA sequencing of 18S rRNA and mitochondrial 16S rRNA confirmed that GPF was derived from the golden pompano. Chromosomal analysis revealed that the number pattern of GPF was 48 chromosomes. Transfection experiments demonstrated that GPF could be utilized to express foreign genes. Furthermore, heavy metals (Cd, Cu, and Fe) exhibited dose-dependent cytotoxicity against GPF. After polyinosinic-polycytidylic acid (poly I:C) treatment, transcription of the retinoic acid-inducible gene I-like receptor (RLR) pathway genes, including mda5 , mita , tbk1 , irf3 , and irf7 increased, inducing the expression of interferon (IFN) and anti-viral proteins in GPF cells. In addition, lipopolysaccharide (LPS) stimulation up-regulated the expression of inflammation-related factors, including myd88 , irak1 , nfκb , il1β , il6 , and cxcl10 expression. To the best of our knowledge, this is the first study on the immune response signaling pathways of the golden pompano using an established fin cell line. In this study, we describe a preliminary investigation of the GPF cell line immune response to poly I:C and LPS, and provide a more rapid and efficient experimental material for research on marine fish immunology. • GPF was established and became a stable fin cell line of Trachinotus blochii. • GPF can be used to assess the cytotoxicity of heavy metals. • GPF can be utilized to express foreign genes with 32 % transfection efficiency. • GPF's immune response to LPS and poly I: C challenge proved it can be used for in vitro immunology study. [ABSTRACT FROM AUTHOR]