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porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV) infection, is an important swine infectious disease that causes substantial losses worldwide each year. PRRSV is a positive-sense single-stranded RNA virus that is highly susceptible to mutation and recombination, making vaccine and drug research for the disease extremely difficult. In this study, the binding of PRRSV nsp2 to HSP71 protein was detected by using the IP/MS technique. And the inhibitory effect of HSP71 on nsp2 antagonistic activity was validated by measuring NF-kB luciferase reporter. According to stress from inhibitory effects, the amino acid variation profile of PRRSV nsp2 under HSP71 stress was further analyzed using second-generation sequencing. Surprisingly, the results indicated that HSP71 pressure limits the random mutations of PRRSV nsp2 and maintains the dominant PRRSV strain within the population. Mutant strain showed weaker antagonistic activity and replication capability in cell. These results imply the binding of HSP71 with PRRSV nsp2 may lead to maintain the stability of highly virulent strains of PRRSV. • Hsp71 binds and inhibits PRRSV nsp2. • hsp71 binding contributes to the stabilization of PRRSV nsp2. • PRRSV nsp2 is prone to random mutations in normal cells. [ABSTRACT FROM AUTHOR]

Thermal interface materials (TIMs) with high through-plane thermal conductivity (TC), softness, and electrical insulation are highly desired for modern electronics. However, it is challenging to simultaneously achieve these properties. Boron nitride (BN)-based polymer composites are promising candidates for advanced TIMs owing to the high TC and great electrical insulation of BN. However, previous works perform either low TC (< 8 W m−1 K−1) or high stiffness. The ultimate properties of BN-based TIMs remain largely unclear and unrealized. Here, we fabricate BN film-filled silicone rubber composites by a facile stacking-cutting method, which maintains the high degree of orientation the BN film, so that a record-high though-plane TC of 19.1 W m−1 K−1 and a low compressive modulus of 5.42 MPa are achieved. The low BN content (37 vol%) ensures the softness and resilience of the as-prepared TIMs. This work presents a highly efficient strategy to enhance the performance of BN based TIMs, promoting their large-scale manufacturing and practical applications. • Thermal interface materials based on boron nitride film (BNF) were fabricated by a facile stacking-cutting method. • The composites exhibit a high degree of orientation and densely overlapped structures. • The composites show a record-high though-plane TC of 19.1 W m−1 K−1 at 37 vol% BN content and a low compressive modulus of 5.42 MPa. [ABSTRACT FROM AUTHOR]

The widespread application and interest in awake prone positioning stems from its ease and availability and its ability to enhance patients' oxygenation. Nevertheless, due to the absence of consensus over the regimen of awake prone positioning, the efficacy of awake prone positioning remains uncertain. To explore the optimal regimen for awake prone positioning, including the timing of initiation, ideal daily duration, and strategies for improving patient comfort and encouraging adherence. Retrospective observational study. Two university-affiliated hospitals in Shanghai. Between December 2022 and February 2023, a total of 475 patients with COVID-19-related pneumonia who received awake prone positioning were observed. The data were collected from the hospital's electronic medical record system. The differentiation efficiency of peripheral blood oxygen saturation [SpO 2 ]:fractional oxygen concentration in inspired air [FiO 2 ] ratio at first awake prone positioning for different outcomes was tested by the area under the receiver operating characteristic curve. The Cox proportional hazard regression model was used to analyze the relationship between time to occurrence of 28-day outcomes and collected variables. Kaplan–Meier curves were plotted with the percentage of 28-day outcomes according to the SpO 2 :FiO 2 ratio at first awake prone positioning after controlling covariates through Cox regression. The best efficiency in predicting patient outcomes was achieved when the cutoff SpO 2 :FiO 2 ratio at first awake prone positioning was 200. Patients with a reduced SpO 2 :FiO 2 ratio (≤ 200) experienced more adverse respiratory outcomes (RR = 5.42, 95%CI [3.35, 8.76], p < 0·001) and higher mortality (RR = 16.64, 95%CI [5.53, 50.13], p < 0.001). Patients with a SpO 2 :FiO 2 ratio of ≥ 200 at first awake prone positioning, longer duration between first awake prone positioning and admission, more awake prone positioning days, and better awake prone positioning completion were significantly protected from 28-day adverse respiratory outcomes and mortality. Initiating awake prone positioning with a SpO 2 :FiO 2 ratio exceeding 200, increasing the number of awake prone positioning days, prolonging the time between first awake prone positioning and admission, and achieving better completion of awake prone positioning were found to be significantly associated with reduced adverse respiratory outcomes and mortality. ClinicalTrials.gov ; No.: NCT05795751 ; URL: www.clinicaltrials.gov. [ABSTRACT FROM AUTHOR]