Search

In HR+ /HER2- breast cancer patients with ≤ 3 positive axillary lymph nodes (ALNs), genomic tests can streamline chemotherapy decisions. Current studies, centered on tumor metrics, miss broader patient insights. Automated Breast Volume Scanning (ABVS) provides advanced 3D imaging, and its potential synergy with radiomics for ALN evaluation is untapped. This study sought to combine ABVS radiomics and clinical characteristics in a nomogram to predict ≤ 3 positive ALNs in HR+ /HER2- breast cancer patients with 1–2 positive sentinel lymph nodes (SLNs), guiding clinicians in genetic test candidate selection. We enrolled 511 early-stage breast cancer patients: 362 from A Hospital for training and 149 from B Hospital for validation. Using LASSO logistic regression, primary features were identified. A clinical-radiomics nomogram was developed to predict the likelihood of ≤ 3 positive ALNs in HR+ /HER2- patients with 1–2 positive SLNs. We assessed the discriminative capability of the nomogram using the ROC curve. The model's calibration was confirmed through a calibration curve, while its fit was evaluated using the Hosmer–Lemeshow (HL) test. To determine the clinical net benefits, we employed the Decision Curve Analysis (DCA). In the training group, 81.2% patients had ≤ 3 metastatic ALNs, and 83.2% in the validation group. We developed a clinical-radiomics nomogram by analyzing clinical characteristics and rad-scores. Factors like positive SLNs (OR = 0.077), absence of negative SLNs (OR = 11.138), lymphovascular invasion (OR = 0.248), and rad-score (OR = 0.003) significantly correlated with ≤ 3 positive ALNs. The clinical-radiomics nomogram, with an AUC of 0.910 in training and 0.882 in validation, outperformed the rad-score-free clinical nomogram (AUCs of 0.796 and 0.782). Calibration curves and the HL test (P values 0.688 and 0.691) confirmed its robustness. DCA showed the clinical-radiomics nomogram provided superior net benefits in predicting ALN burden across specific threshold probabilities. We developed a clinical-radiomics nomogram that integrated radiomics from ABVS images and clinical data to predict the presence of ≤ 3 positive ALNs in HR+ /HER2- patients with 1–2 positive SLNs, aiding oncologists in identifying candidates for genomic tests, bypassing ALND. In the era of precision medicine, combining genomic tests with SLN biopsy refines both surgical and systemic patient treatments. [ABSTRACT FROM AUTHOR]

Phallus rubrovolvatus (P. rubrovolvatus) is a large and rare fungus widely consumed and used for medicinal purposes in East Asian countries, earning the reputation of "plant meat". We created a reference genome for P. rubrovolvatus , with an assembly length of 33.39 Mb and 144 overlapping groups. We identified 9,204 coding genes on the genome, of which 231 gene clusters are related to amino acid synthesis and transport. In this study, via integrating the flavor-related metabolite and transcriptome data of five different developmental stages of P. rubrovolvatus , we produced a global map of the alterations in flavor-related metabolites throughout the entire development process of P. rubrovolvatus. With this dataset, we constructed a complex regulatory network, thereby identifying the key structural genes and metabolites for the regulation of free amino acids in the flavor formation of P. rubrovolvatus , and glutathione and 5′-nucleotides possess a strong synergistic fresh-enhancing effect. In the five different developmental stages of P. rubrovolvatus , the regulation of the identified genes related to the synthesis of amino acids and derivatives on flavor metabolism has been confirmed, which demonstrates that our dataset offers a suitable instrument for elucidating the regulatory substances that control the flavor biosynthetic pathways, which were previously not elucidated. Overall, our research findings not only provide novel insights into the metabolic regulation of flavor during the mycelia developmental process of P. rubrovolvatus , but also lay the groundwork for enhancing the flavor of P. rubrovolvatus. [Display omitted] • The genome of Phallus rubrovolvatus has been assembled. • Amino acids grant Phallus rubrovolvatu s a unique flavor. • Glutathione and 5′-nucleotide play an enhancing role in flavor formation. • The molecular mechanism of the formation of the unique flavor of Phallus rubrovolvatu s. [ABSTRACT FROM AUTHOR]