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This paper aims to identify the factors that are positively or negatively impacting students' well-being and their academic engagement. We used partial least-squares structural equation modeling (PLS-SEM) using the data collected through a questionnaire from four countries: Romania, Turkey, Slovakia, and Bulgaria. The model includes seven factors that influence the well-being of students and indirectly their academic engagement: stressors in the students' lives; professors' support; social support from family and friends; the students' perceived satisfaction in their lives; engaging in activities during their leisure time; self-exploration regarding their careers; and environmental exploration regarding their careers. The results show that all factors, except for stressors and environmental exploration regarding their careers, positively influence the students' well-being and thus their academic engagement. These findings are useful for university professors and managers in better organizing activities to increase academic performance. [ABSTRACT FROM AUTHOR]

The digital transformation of society is a reality. Technology is becoming integral to people's daily lives and children are growing up in the digital age. The education systems have to reflect and adapt to the new digital reality to effectively engage and prepare students for the future. Teachers have to possess digital competences at a high level, meaning that they are successfully able to use digital tools and technologies in their teaching practices. Digital competences include digital literacy, experience using educational software and platforms, and the ability to seamlessly integrate information and communication technologies (ICT) into the organization of educational activities. The current paper aims to study the opinion of teachers in Bulgarian schools regarding the possibilities of using digital technologies to achieve educational goals and the extent to which teachers integrate digital tools in their teaching activities. Teachers' opinions about the need for specific digital competences are important. The results of the survey show that teachers in Bulgaria actively use digital technologies in their daily teaching activities (84.8%) in order to create and provide learning content to students and to provoke their active participation, and is a convenient tool for evaluating their students' knowledge and skills and for providing effective feedback. The conducted research also identifies the necessary competences that teachers of the digital generation of learners need—skills and competences for developing authored electronic resources in various formats for students with different learning styles, as well as for working in online environments—individually and collaboratively with other teachers. [ABSTRACT FROM AUTHOR]

The principal economic sector devoted to the breeding, raising, and production of farm animals is known as the livestock industry. There are precise standards for making high-quality feed in animal husbandry. Precision livestock feeding is a crucial component, with the potential to significantly impact the profitability of livestock; it permits the provision of diets to animals that are precisely tailored to their specific daily nutritional needs. Through simulation modeling, a single model can be created for automated systems to determine daily rations for farm animals. For the purposes of this document, precision livestock feeding refers to the practice of tailoring feed to individual animals or groups of animals, taking into account their changing nutritional needs over time and individual differences in terms of nutritional requirements. The practice aims to optimize animal health and performance while reducing feed waste. This paper presents a formal model for determining the quantities of components needed to achieve a minimum cost mixture that satisfies compositional and quantitative criteria. The present research calculates the amount of hay and silage required to feed an animal per day at the most economical cost by applying an optimization approach that involves defining and solving an optimization problem. The problem is solved using a well-known software package, which is necessary for the practical application of the resulting model. Real data from livestock production in Bulgaria are used to numerically test the model. [ABSTRACT FROM AUTHOR]

This paper discusses the application of digital information management tools in the context of financial control. In Bulgaria, such research is innovative as it is the first time that digital transformation in crucial financial control institutions, which influence the formation of the revenue part of the state budget and the spending of public funds, has been studied. The study aims to answer the research question of to what extent the application of digital tools in financial control improves its effectiveness. It analyses how modern technologies improve the efficiency and accuracy of information used in financial control institutions. The authors examine the impact of digital tools, such as database management systems, business analytics platforms, and electronic document management tools, on collecting, analyzing, and managing financial and non-financial information. The study uses descriptive statistics and a correlation analysis, which significantly contributes to establishing the relationship between implemented digital tools and improvements in financial control procedures. The results show that despite the conditions created for digitalization in financial control institutions, digital tools are used to a limited extent in the information management process. The study emphasizes the need for continuous investment in digital technologies and training to maximize the benefits of their application in financial control. [ABSTRACT FROM AUTHOR]

Rainfall, with its intensity, duration, and seasonal distribution, is among the main factors causing soil erosion, which is a widespread environmental problem in Bulgaria. Rainfall erosivity shows the potential of precipitation to generate erosion processes and is an essential indicator of the climate vulnerability of a region. This paper aims to evaluate rainfall erosivity in a part of the Eastern Rhodopes Mountains, an area that is characterised by high-intensity erosion processes and high erosion risk. Local peculiarities of rainfall erosivity were revealed by the calculation of some precipitation indices based on the monthly precipitation for the period 2000–2021, such as the precipitation concentration index (PCI), Angot precipitation index, Fournier index (FI), and modified Fournier index (MFI). The analysis of the extremely wet and extremely dry months at the annual and seasonal (October–March and April–September) levels was performed to evaluate the susceptibility to erosion. The results from the study show that rainfall erosivity in the studied area varies from low to moderate in the northern part of the study area and from high to very high in the south. According to the MFI, high and very high erosivities have been observed mainly since 2012. The erosivity increases from north to south, to the area with a complex relief, where the combination of orography and atmospheric circulation make favourable conditions for the occurrence of extreme precipitation. The analyses of the calculated indices show that the precipitations in most of the studied area generally have from a low to a moderate erosivity, but this does not exclude the occurrence of cases with high and very high erosivities, which are characteristic of recent years and are related to the increase in annual precipitations and extreme precipitation months. The results of this study can contribute to the development and implementation of measures and preventive activities for the reduction and possible elimination of the negative impacts of extreme precipitation. [ABSTRACT FROM AUTHOR]

Valorization of high-volume mine tailings could be achieved by the development of new geopolymers with a low CO2 footprint. Materials rich in aluminum and silicon with appropriate solubility in an alkaline medium can be used to obtain a geopolymer. This paper presents a study of copper mine tailings from Bulgaria as precursors for geopolymers. Particle size distribution, chemical and mineralogical composition, as well as alkaline reactivity, acidity and electroconductivity of aqueous slurry are studied. The heavy metal content and their mobility are studied by leaching tests. Sequential extraction was applied to determine the geochemical phase distribution of heavy metals. The studied samples were characterized by high alkalinity, which could favor the geopolymerization process. The water-soluble sulphates were less than 4%. The Si/Al ratio in mine tailing was found to be 3. The alkaline reactivity depended more so on the time of extraction than on the concentration of NaOH solution. The main part of the heavy metals was found in the residual fraction; hence, in high alkaline medium during the geopolymerization process, they will stay fixed. Thus, the obtained geopolymers could be expected to exert low environmental impact. The presented results revealed that studied copper mine tailing is a suitable precursor for geopolymerization. [ABSTRACT FROM AUTHOR]

In recent decades, climate change has become a critical global issue with far-reaching consequences for regional climates and ecosystems. While regional climate models provide valuable information, there is a growing need for high-resolution simulations to assess local impacts. This paper addresses this gap by presenting the first simulation of a 3 km convection-permitting (CP) scenario simulation for Bulgaria. The main aim of this study is to assess different precipitation indices and their future changes for Bulgaria under the Representative Concentration Pathway 8.5 (RCP8.5) scenario following the Coordinated Regional Climate Downscaling Experiment Flagship Pilot Study protocol. The simulations are evaluated against high-resolution observations. We downscale Coupled Model Intercomparison Project 5 Global Climate Model (CMIP5 GCM) data for historical (1995–2004) and future (2089–2098) periods using a regional climate model (RCM) at 15 km grid spacing and parametrized convection. We use these fields as initial and boundary conditions for convection-permitting kilometer-scale simulations. The 15 km grid spacing driving model is used as a reference to assess the added value of the kilometer-scale simulation. Additionally, the 3 km seasonal mean and projected 2 m temperature and the winter snow water equivalent are presented. The results show that the kilometer-scale simulation shows better performance of wet-hour intensity in all seasons, wet-hour frequency in the spring, fall, and winter, and extreme precipitation (99.9th percentile of all precipitation events, p99.9) in the winter and fall. The kilometer-scale simulation improves the projected precipitation distribution and modifies the signal of the precipitation frequency, intensity, and heavy precipitation change over some areas. A positive projected change in the wet-hour intensity is expected in all seasons (13.86% in spring, MAM, 17.48% in summer, JJA, 1.97% in fall, SON, and 17.43% in winter, DJF) and in the heavy precipitation in the spring (13.14%) and winter (31.19%) in the kilometer-scale experiment. The projected increase in mean winter precipitation is accompanied by a significant decrease in mean winter snowfall over lowlands (50−70%). The convection-permitting Regional Climate Model, version 4.7.1 (RegCM4.7.1) suggests an increase in winter snowfall over the highest parts of the country, but a significant increase in the 2 m temperatures there. The results of this study are encouraging and may be of interest to the community of climate scientists and users of climate data for making reliable estimates of the local impacts of future climate change. [ABSTRACT FROM AUTHOR]

Assisting women in attaining their reproductive goals is crucial for improving the well-being of families and children. As the first point of contact for healthcare, general practitioners (GPs) are ideal for family planning (FP) and preconception care (PCC). However, primary care interventions' efficacy is unclear. The aim of this study was to examine GPs' knowledge, attitudes, and perspectives on FP and PCC service management. Most GPs were aware of FP and PCC services and held a firm conviction that they should be primarily accountable together with obstetrician–gynaecologists. However, it is worth noting that less than 50% of respondents reported receiving thorough and comprehensive knowledge of their respective specialities. Those with general medicine qualifications demonstrated a high level of commitment to providing such services. The women's GPs and those with training in general medicine prescribed birth control pills and emergency contraception three times more frequently than the other doctors who suggested condoms or traditional methods or referred patients to another specialist (p < 0.05). In conclusion, PCC is of the utmost importance, and its effective implementation demands the collaboration of policymakers, healthcare providers, and individuals. GPs are essential in managing FP and PCC. They must incorporate more in-depth PCC into their clinical practice. [ABSTRACT FROM AUTHOR]

This study aims at the identification and characterization of five actinobacterial strains with presumed belonging to the species Curtobacterium flaccumfaciens isolated from tomato and pepper plants, and establishing the potential role of both plants as natural reservoirs of this phytopathogen. Species identification was performed via MALDI-ToF MS, 16S rDNA sequencing and PCR. The strains were Gram-positive with a coryneform cell shape having yellow/orange-pigmented colonies; positive for catalase and esculin, and starch and casein hydrolysis; oxidase-, urease-, indole- and nitrate-reduction-negative and were strictly aerobic. All isolates produced antimicrobial substances against various phytopathogenic bacteria. Tomato and pepper plants were artificially infected with newly isolated strains in order to establish their role as natural reservoirs of the bacteria. Morphological alterations were observed only in the tomato plants, with defoliation of the first two to four leaves at the 28th day. Then, viable coryneform bacterial isolates (n = 73) were successfully re-isolated only from the stems of the infected plants. The similarity between the re-isolates and the respective initial isolates was confirmed phenotypically and genotypically by RAPD-PCR, confirming that solanaceous vegetables can act as reservoirs of C. flaccumfaciens. This is the first report of C. flaccumfaciens in Bulgaria. [ABSTRACT FROM AUTHOR]

For more than a century, in the center of Sofia, the capital of the Republic of Bulgaria, there is and functions the church of St. Nikolai Mirlikiyski the Wonderworker, known as the Russian church. The church was built at the beginning of the 20th century with the idea of being part of the complex of the Russian imperial diplomatic body in Sofia and to serve the Russian diplomats, their families and the Orthodox Russian citizens living permanently or temporarily in the Bulgarian capital. However, after its consecration in 1914, disputes began, both regarding the ownership of the church building and the canonical jurisdiction of the church—of the Metropolitan of Sofia or the Holy Synod of the Russian Orthodox Church in Moscow. This dispute culminated in September 2023, with the expulsion from Bulgaria of several Russian and Belarusian clergies serving in the Russian church on charges of espionage in favor of the Russian Federation. The subsequent closure of the church by the Russian ambassador Mitrofanova led to internal and external political tension and ecclesiological chaos in the country. The Russian side violated the Bulgarian Law on Religions, known as the Confessions Act 2002, and directly infringed the Statute of the Bulgarian Orthodox Church. This article provides expert answers to the public law, property rights and canonical issues concerning the case of the Russian church in Sofia, based on the relevant sources of law (ecclesiastical and civil). [ABSTRACT FROM AUTHOR]

A number of Phytophthora species have been identified as destructive plant pathogens and invasive species. They have the potential to affect a wide range of host plants and cause diseases in agricultural and forest ecosystems. Two Phytophthora species from rhizosphere soil samples collected from declining Alnus glutinosa in Bulgaria were isolated in the autumn of 2022. They were identified as Phytophthora polonica and Phytophthora hydropathica according to the DNA sequence analysis of the ITS region, as well as their morphological and physiological characteristics. The pathogenicity of both species to common and gray alder was evaluated by the inoculation of detached leaves and cuttings. Experimental data proved that P. polonica and P. hydropathica are able to cause leaf necrosis not only on A. glutinosa from which they were derived, but also on A. incana. No significant deference in the aggressiveness of the studied isolates from both Phytophthora species against the two tested plants was observed. Therefore, P. polonica and P. hydropathica were determined as potential threats for alder ecosystems in the country. This is the first report for the isolation of P. polonica in Bulgaria and represents the most southeastern point of the species distribution in Europe. [ABSTRACT FROM AUTHOR]

The efficient planning and control of timber harvesting operations is crucial to growth productivity and reducing costs, and different systems or methods are applied to obtain elevated performance. In particular, cable yarding is a highly appreciated and very efficient wood extraction method in areas that are difficult to access with ground-based extraction machines. Combined logging systems are not diffuse and not often implemented. For this reason, the use and the application of innovative methods are limited. However, some combinations have recently been observed in forest operations. This study, conducted in a deciduous stand in Bulgaria, paid attention to the combination of a tractor-mounted tower yarder (Valentini V400) with a clambunk skidder (Timberjack 1010D), aiming to examine the viability and develop the operational efficiency of the two-stage extraction system and to define the time, log volume extracted per unit by the yarder and the clambunk, and the yarding and skidding costs. The researchers carried out a time-motion study and performed a regression analysis to identify those variables that are most likely to affect the duration of work elements and productivity. For cost analysis, the COST model was used. The mean productivity of the tower yarder was 10.34 m3/PMH and 8.11 m3/SMH, while for the clambunk skidder, the productivity was 6.23 m3/PMH and 4.93 m3/SMH. The net costs for the combined logging system were calculated as 120.87 €/PMH and 14.93 €/m3. This study adds new data about the correct use of both machines, supporting their application in beech forests. [ABSTRACT FROM AUTHOR]

The objective of the present study is to analyze the link between the degree of professional burnout among general practitioners in Bulgaria during a pandemic and their satisfaction with the balance between their personal lives and professional lives. A cross-sectional study was conducted during the fourth wave of the COVID-19 pandemic from December 2021 to January 2022 among 377 general practitioners. We identified the presence and level of burnout syndrome among GPs using V. Boyko's method for diagnostics of the severity of symptoms and the phases of formation and completion of the 'occupational burnout' process. Descriptive statistics and parametric and non-parametric tests were used for the analysis. For the significance level of the null hypothesis, we assumed that p < 0.05 at a 95% confidence interval. A total of 96.3% of the GPs had a high level of burnout during the COVID-19 pandemic. They worked more than 8 h a day (74.8%) and more than 5 days a week (69.0%). A total of 86.3% of them were not satisfied with the spare time they had and stated that they could not balance their work and personal lives (67.1%) since the pandemic was announced. A link was found between the level of professional burnout and long working hours (p = 0.022), dissatisfaction with free time (p = 0.028), and the inability to balance work and personal life (p = 0.000), as well as concerns related to safety during the pandemic (p = 0.048). Unrealistically high levels of burnout during the COVID-19 pandemic due to a disturbed work–life balance is a reason to re-evaluate health policies and involve more hospital care doctors at the frontlines to fight against severe infectious diseases. The results of this study could be used to inform policy makers, healthcare managers, and other stakeholders about the factors that have had profound impacts on GPs' stress levels during the COVID-19 pandemic. [ABSTRACT FROM AUTHOR]

(1) Introduction: The subject of the present study concerns the analysis of the existence and long time evolution of the solar ~200 yr (de Vries/Suess) and ~2400 yr (Hallstadt) cycles during the recent part of the Wurm ice epoch and the Holocene, as well as their forcing on the regional East European climate during the last two calendar millennia. The results obtained here are compared with those from our previous studies, as well as with the results obtained by other authors and with other types of data. A possible scenario of solar activity changes during the 21st century, as well as different possible mechanisms of solar–climatic relationships, is discussed. (2) Data and methods: Two types of indirect (historical) data series for solar activity were used: (a) the international radiocarbon tree ring series (INTCAL13) for the last 13,900 years; (b) the Schove series of the calendar years of minima and maxima and the magnitudes of 156 quasi 11 yr sunspot Schwabe–Wolf cycles since 296 AD and up to the sunspot cycle with number 24 (SC24) in the Zurich series; (c) manuscript messages about extreme meteorological and climatic events (Danube and Black Sea near-coast water freezing), extreme summer droughts, etc., in Bulgaria and adjacent territories since 296 and up to 1899 AD, when the Bulgarian meteorological dataset was started. A time series analysis and χ2-test were used. (3) Results and analysis: The amplitude modulation of the 200 yr solar cycle by the 2400 yr (Hallstadt) cycle was confirmed. Two groups of extremely cold winters (ECWs) during the last ~1700 years were established. Both groups without exclusion are concentrated near 11 yr sunspot cycle extremes. The number of ECWs near sunspot cycle minima is about 2 times greater than that of ECWs near sunspot cycle maxima. This result is in agreement with our earlier studies for the instrumental epoch since 1899 AD. The driest "spring-summer-early autumn" seasons in Bulgaria and adjacent territories occur near the initial and middle phases of the grand solar minima of the Oort–Dalton type, which relate to the downward phases and minima of the 200 yr Suess cycle. (4) Discussion: The above results confirm the effect of the Sun's forcing on climate. However, it cannot be explained by the standard hypothesis for total solar irradiation (TSI) variations. That is why another hypothesis is suggested by the author. The mechanism considered by Svensmark for galactic cosmic ray (GCR) forcing on aerosol nuclei was taken into account. However, in the hypothesis suggested here, the forcing of solar X-ray flux changes (including solar flares) on the low ionosphere (the D-layer) and following interactions with the Earth's lithosphere due to the terrestrial electric current systems play a key role for aerosol nuclei and cloud generation and dynamics during sunspot maxima epochs. The GCR flux maximum absorption layer at heights of 35–40 km replaces the ionosphere D-layer role during the sunspot minima epochs. [ABSTRACT FROM AUTHOR]

In order for a strain to be considered a probiotic or suitable plant bioprotective agent, it must have proven antimicrobial activity against pathogenic bacteria and phytopathogenic fungi. Bacillus amyloliquefaciens M exhibited significantly high antifungal activity against pathogenic fungi of the genera Aspergillus, Penicillium, Fusarium, and Sclerotinia; yeasts of the genera Candida and Saccharomyces; as well as high antibacterial activity against pathogens of the genera Escherichia, Salmonella, Staphylococcus, Listeria, Pseudomonas, and Bacillus. The manifested antimicrobial activity was influenced by the composition of the growth medium. The antifungal activity of the strain was investigated at growth temperatures of 30 °C and 37 °C, and at different pH values in aerobic and anaerobic cultivation, under static and dynamic culturing conditions. High antifungal activity was observed at the 24th h on both growth media (LBG broth and MPB broth) at pH = 6 and pH = 7 in aerobic and anaerobic cultivation. Bacillus amyloliquefaciens M produced antibiotic substances at pH > 5.0, and the antibiotic substances were either secreted into the medium or associated with the cell surface. Four compounds with different antifungal activity and different Rf values were registered through thin-layer chromatography (Rf1 = 0.47; Rf2 = 0.55; Rf3 = 0.67; and Rf4 = 0.75), two of the compounds were ninhydrin-positive. Bacillus amyloliquefaciens M was cultured in a bioreactor with stirring, and the parameters of the growth kinetics and the sporulation kinetics have been modeled. A spore concentrate of Bacillus amyloliquefaciens M has been obtained. In further research, the efficiency of the concentrate as a plant bioactive agent will be tested. [ABSTRACT FROM AUTHOR]

Rosa centifolia L. and Rosa gallica L. (Rosaceae) are grown as raw materials for valuable essential oils and hydrosols. There are scarce data about the biological activities and the genoprotective potential of the hydrosols of these roses. The aim of the study was to provide information on their cytotoxic/genotoxic activity and anti-cytotoxic/anti-genotoxic capacity against mutagenic N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). The evaluation was performed using classical tests for chromosomal aberrations and micronuclei in the higher plant Hordeum vulgare and human lymphocyte test systems. The experimental schemes included combined hydrosol and mutagen treatment. Both hydrosols (6, 14, 20%) had no cytotoxic effect on barley and showed low genotoxicity in both test systems as the injuries were enhanced to a lesser extent compared to the controls. Lymphocytes were more susceptible than H. vulgare. Under the conditions of combined treatment, it was found that the two hydrosols possessed good anti-cytotoxic and anti-genotoxic potential against MNNG. Both rose products exerted genoprotective potential to a similar extent, decreasing the frequencies of aberrations in chromosomes and micronuclei to a significant degree in both types of cells when non-toxic concentrations of hydrosols were applied before MNNG. This was performed both with and without any inter-treatment time. The observed cytoprotective/genoprotective potential suggests that these hydrosols are promising for further application in phytotherapy and medicine. [ABSTRACT FROM AUTHOR]

The present research aimed to characterize the genetic diversity and relationships among extant Arabian horse sire lines in Bulgaria, using 15 equine microsatellite markers. The evaluation included 537 Arabian horses representing nine sire lines (SAKLAWI I, LATIF, SEANDERICH, IBRAHIM, SHABAB, DJEBEL MOUSA, KUHAILAN AFAS, BAIRACTAR, and SARHAN). The obtained results indicated that within these lines, the mean number of alleles ranged from 4.15 in SARHAN to 5.54 in SAKLAWI I and LATIF. The mean expected heterozygosity (He) ranged from 0.54 in the SEANDERICH line to 0.67 in SAKLAWI I. The inbreeding coefficient for the entire Arabian populations was rather low: FIS = −0.109, fluctuating from −0.204 in SHABAB to −0.041 in SAKLAWI I. The mean genetic differentiation, FST, was 0.096, demonstrating that nearly 90% of the total genetic variation was due to genetic differentiation within each population. STRUCTURE analysis indicated a genetic similarity between SHABAB and LATIF, between IBRAHIM and KUHAILAN AFAS, as well as between SAKLAWI I, SEANDERICH, and BAIRACTAR. This study of the genetic diversity of Arabian sire lines in Bulgaria can assist in developing a national strategy for the exclusion of non-purebred animals from breeding programs in order to preserve the genetic profile of the original Arabian lines. [ABSTRACT FROM AUTHOR]

In recent years, a significant import of modern tower yarders has been registered in Bulgaria, where official productivity standards are routinely used for operational planning and control. Given the higher potential of the newer yarder models, the Bulgarian forestry sector has started a review of the older productivity standards dating back to the 1970s. This new endeavor has offered an ideal opportunity for gauging the effect of technological progress in yarder technology. Therefore, the authors have used the very first results achieved during the development of the new standards for conducting a preliminary quantitative comparison between older and newer yarder types. Modern yarders (e.g., Konrad Mounty 4000) are much faster than the older ones (e.g., Koller K300), and their time consumption per cubic meter is half as large, especially on longer distances. At short distances, however, their performance evens out. Regardless of the distance, the installation time of the Konrad Mounty 4000 is twice as short. As they are largely automated, the new machines can be manned by smaller crews (e.g., two workers instead of three) and are easier and safer to operate. Finally, the new machines are equipped with built-in loaders and processors, which allows them to integrate delimbing, crosscutting and stacking within the same work cycle. With older models, a separate team must be deployed for those tasks. [ABSTRACT FROM AUTHOR]

Simple Summary: In this study, we investigate the availability and access to orphan drugs for rare cancers in Bulgaria, aiming to address the urgent need for improved treatment access for this vulnerable population. By comparing data from European and national sources, we aimed to assess the availability, delays, and budgetary impact of these drugs. Our findings reveal significant disparities in their access and highlight the pressing need for targeted policies to address these inequalities. This research contributes valuable insights into the challenges faced by rare cancer patients and we call for focused efforts at both the European and national levels to ensure equitable access to treatment. Rare cancers are defined by an annual incidence of fewer than 6 per 100,000. Bearing similarities to rare diseases, they are associated with substantial health inequalities due to diagnostic complexity and delayed access to innovative therapies. This situation is further aggravated in Southeastern European countries like Bulgaria, where limited public resources and expertise underscore the need for additional policy and translational research on rare cancers. This study aimed to explore the availability and access to orphan drugs for rare cancers in Bulgaria for the period of 2020–2023. We cross-compared data from both the European Union and national public sources to evaluate the number of available and accessible orphan drugs for rare cancers, the delay from market authorization to reimbursement, the dynamics of public expenditures, and regional disparities in access across the country. We juxtaposed the main characteristics of oncological and non-oncological orphan drugs as well. Only 15 out of 50 oncological orphan drugs that were authorized by the European Medicine Agency were accessible for rare cancer patients in Bulgaria. The median delay between market authorization and inclusion in the Bulgarian Positive Drug List was 760 days. The total expenditures for all orphan drugs for rare cancers amounted to EUR 74,353,493 from 2020 to 2023. The budgetary impact of this group rose from 0.24% to 3.77% of total public medicinal product expenditures for the study period. Rare cancer patients represent a vulnerable population that often faces limited to no access to treatment. We call for targeted European and national policies to address this major inequality. [ABSTRACT FROM AUTHOR]

In the present contribution, we report the results from a study on the ancient technology used to create decorative color plasters in the Thracian tomb near the village of Alexandrovo, Bulgaria. A series of fragments of red, black, grey, white and brown colored lime plasters from the dromos and funeral chamber were investigated by laser-induced breakdown spectroscopy, X-ray diffraction analysis, infrared spectroscopy, paramagnetic electron resonance spectroscopy and differential scanning calorimetry. Based on the combined analytical data, it was possible to identify the pigments, fillers and other materials in the composition of the decorative plasters in the interior, as well as to clarify the technological features related to the plaster creation. The results demonstrated that the murals were implemented on two layers in the case of white, black, grey and brown decoration—first coarse mortar, followed by a white, fine mortar, which usually was made of calcite. In the case of red decoration, a pigment was added to the fine mortar to achieve a colored surface. The pigments were identified as mostly traditional mineral pigments—calcite, kaolinite, red natural ochres (colored earth), brown colored earth and black pigment (amorphous C). The use of the fresco technique is implied by the major participation of calcite and the absence of organic binder in all of the painting layers. [ABSTRACT FROM AUTHOR]

The migration of 32 elements from natural zeolitized tuffs from the Beli Plast and Golobradovo deposits (Bulgaria) was determined in ultrapure, tap, mineral, and coal mine waters in order to evaluate their desorption and adsorption properties. The tuffs are Ca-K-Na and contain clinoptilolite (90 and 78wt.%, respectively), plagioclase, sanidine, opal-CT, mica, quartz, montmorillonite, goethite, calcite, ankerite, apatite, and monazite. The desorption properties are best revealed during the treatment of ultrapure, tap, and mineral water, whereas the adsorption properties are best manifested in coal mine water treatment. The concentrations of Al, Si, Fe, Na, Mn, F, K, Pb, and U increase in the treated ultrapure, tap, and mineral water, while the content of K, Be, Pb, and F increase in the treated mine water. The tuffs show selective partial or complete adsorption of Na, Mg, Sr, Li, Be, Mn, Fe, Co, Ni, Cu, Zn, Al, Pb, U, and SO42−. They demonstrate the ability to neutralize acidic and alkaline pH. Sources of F are presumed to be clinoptilolite and montmorillonite. The usage of zeolitized tuffs for at-home drinking water treatment has to be performed with caution due to the migration of potentially toxic and toxic elements. [ABSTRACT FROM AUTHOR]

Crithmum maritimum L. is a perennial halophyte plant that is a medicinal herb known by people from different cultures since ancient times. However, the therapeutic potential of this halophyte has not been completely investigated, and the scientific data on it are limited. The purpose of the present study was to estimate the chemical composition of the essential oil (EO) obtained from the aerial parts of Crithmum maritimum L. growing wild in Bulgaria, as well as the histolocalization of secretory structures for the synthesis and accumulation of volatile oils. The results obtained on the EO composition of Bulgarian Crithmum maritimum L. provide an opportunity to reveal potential future applications in various fields, such as medicine, pharmacy, agriculture, food, and the cosmetic industry. Gas chromatography with mass spectrometry was performed to assess the chemical profile of the isolated EO. The phenylpropanoid dillapiole was identified as the major compound in the EO, accounting for 34.09% of the total EO. Monoterpene hydrocarbons represented 62.07% of the total oil composition. γ-Terpinene, D-limonene, and β-pinene were the most abundant monoterpene hydrocarbons in the composition of the EO. In addition, histochemical localization of EO in the stem and leaves of Crithmum maritimum L. was carried out. The secretory structures were located in the cortical region of the stem and in the mesophyll tissues of the leaves in the form of secretory ducts. The performed histochemical analysis confirmed the lipophilic nature of the secretion from the duct cells. This is the first report related to the histolocalization and chemical composition of the EO from Bulgarian Crithmum maritimum L. Furthermore, our data indicate some potential possibilities for the evaluation of the therapeutic activity of the EO obtained from this plant species and outline its future applications as a therapeutic agent. Also, the EO from the studied halophyte plant has prominent potential to be used as a biopesticide, which is an environmentally friendly option compared to standard pesticides. [ABSTRACT FROM AUTHOR]

Gene therapies (GTs) have recently emerged as revolutionary personalized therapeutic options. Despite their promising potential, challenges such as uncertainty regarding long-term health benefits and safety, along with extreme price tags, pose significant obstacles to patient access. Within the EU, the European Medicines Agency plays a pivotal role with regards to GT market authorization. However, national authorities are responsible for pricing and reimbursement, which results in fragment patient access within the EU. This study aimed to provide an overview of the complex landscape of post-market authorization accessibility for GT products in Bulgaria, comparing it with neighboring EU countries. We applied a mixed-methods approach, including desk research, public data requests, and list price comparisons. As of 1 April 2023, 14 GTs had a valid market authorization at the EU level. In Bulgaria, Kymriah® was the only GT included in the Positive Drug List (PDL), with an official list price of EUR 335,636.94. Similar results were found in Romania, whereas five GTs were included in Greece's PDL. Additionally, Zolgensma® was found accessible in Bulgaria through an alternative individual access scheme at an estimated price of EUR 1,945,000.00. In conclusion, this study emphasized targeted policy interventions to address health inequalities and to ensure timely access to GTs within the EU. [ABSTRACT FROM AUTHOR]

Throughout history, ceramics have been the most abundant artifacts in archaeological discoveries. Within the Neolithic period in present-day Bulgaria, skilled artisans applied additional materials to decorate their pottery, evolving in composition and application techniques such as painting or incrustation. This study is focused on the investigation of white pigments used in decorating Early and Late Neolithic pottery from Madzherito, Kaloyanovets, and Hadzhidimitrovo—archaeological sites located in the Thracian Valley, Central South Bulgaria, affiliated with the cultural groups of Karanovo I and IV. Thirteen ceramic sherds were investigated through archaeometric techniques, including Fourier-transformed infrared spectroscopy in attenuated total reflection mode (ATR-FTIR) and laser-induced breakdown spectroscopy (LIBS). LIBS data underwent further analysis using principal component analysis (PCA). The results revealed that calcite, enriched with diverse fillers like quartz, clays, feldspars, and metal oxides, was the primary raw material for white decoration throughout the entire period. Talc emerged as an addition to calcite in the paint of two Early Neolithic sherds. The presence of hydroxyapatite and kaolinite in Late Neolithic pottery was also observed. The inclusion of supplementary ingredients in the primary formula for crafting white decorations signifies either the emergence of novel trends in manufacturing techniques or serves as evidence of vessels imported from adjacent territories. [ABSTRACT FROM AUTHOR]

The present study aims to elucidate the metabolomic profile of Arthrospira platensis grown in a bioreactor in Bulgaria. The results show that Arthrospira platensis has a high content of mannose, 137.02 mg g−1, and vitamin A (retinol)—10.3 μg/100 g. High concentrations of calcium, sulfur, and zinc distinguish its elemental composition. The freeze-dried powder contained 15.81 ± 0.45% dietary fiber, 50.16 ± 0.25% total protein content, and 1.22 ± 0.11% total fat content. Among the unsaturated fatty acids with the highest content is α-linolenic acid (25.28%), while among the saturated fatty acids, palmitic acid prevails (22.55%). Of the sterols in the sample, β-sitosterol predominated. There is no presence of microcystins LR, RR, YR, and nodularin. Therefore, Arthrospira platensis grown in a Bulgarian bioreactor is safe for use in the pharmaceutical and food industries. Many of the organic compounds found have applications in medicine and pharmacology and play an important role in biochemical processes in the body. Therefore, Arthrospira platensis grown in Bulgaria has a high potential for use as an independent food supplement or in combination with other natural products. [ABSTRACT FROM AUTHOR]

An experiment was carried out in the Institute of Animal Science—Kostinbrod, Bulgaria, to investigate the growth performance of male layer-type chickens (Lohmann Brown Classic), raised to 6 and 9 weeks of age, to evaluate the economic aspects of this rearing, as well as to present changes in the quality characteristics of the meat during frozen storage. The chickens were reared in a controlled microclimate with an initial stocking density of 9 birds/m2. After 6 weeks of age, fragmentation of the stocking density was applied, and then it diminished to 3 birds/m2. The chickens were slaughtered at 6 and 9 weeks of age. Ten 9-week-old chickens were subjected to carcass analysis. Meat quality parameters (pH, color), degree of proteolysis (free amino groups), and lipid oxidation (content of peroxides and TBARS) were assessed in fresh breast and thigh meat (0 d) and in samples stored for 60 and 120 days at −18 °C in chickens slaughtered at 6 and 9 weeks old. The mean live weight of the male layer-type chickens at 6 weeks was 608.81 g, while the 9-week-old chickens reached 1115.93 g. The feed conversion ratio (FCR) for the whole period of rearing was 2.75. There were no considerable deviations in the meat traits, indicating quality deterioration over the course of the frozen storage. There was a significant increase in the pH of the breast and thighs, reaching maximum values for 60 days of storage in the 6-week-old chicks, while in the 9-week-old birds, pH peaked in the samples stored for 120 days. The changes in the dynamics of pH corresponded to those of proteolysis. There was an increase in lightness (L*), allowing for higher values in the samples stored for 60 days to be reached regardless of the type of meat and age of the chickens. The content of the peroxides increased considerably for 60 days of frozen storage and decreased afterwards. During storage, there was a constant increase in the secondary products of lipid oxidation. Our results indicated that the application of practices such as the fragmentation of stocking density and finding the suitable age for slaughter have significant importance for the profitability of producing meat product from male layer-type chickens. We found that rearing this type of bird until 9 weeks of age resulted in lower costs and higher economic efficiency. [ABSTRACT FROM AUTHOR]

Wildfires are a common disturbance factor worldwide, especially over the last decade due to global climate change. Monitoring postfire forest regrowth provides fundamental information needed to enhance the management and support of ecosystem recovery after fires. The purpose of this study is to propose an algorithm for postfire forest regrowth monitoring using tasseled-cap-derived indices. A complex approach is used for its implementation, for which a model is developed based on three components—Disturbance Index (DI), Vector of Instantaneous Condition (VIC), and Direction Angle (DA). The final product—postfire regrowth (PFIR)—allows for a quantitative assessment of the intensity of regrowth. The proposed methodology is based on the linear orthogonal transformation of multispectral satellite images—tasseled cap transformation (TCT)—that increases the degree of identification of the three main components that change during a fire—soil, vegetation, and water/moisture—and implies a higher accuracy of the assessments. The results provide a thematic raster representing the intensity of the regrowth classes, which are defined after the PFIR threshold values are determined (HRI—high regrowth intensity; MRI—moderate regrowth intensity; and LRI—low regrowth intensity). The accuracy assessment procedure is conducted using very-high-resolution (VHR) aerial and satellite data from World View (WV) sensors, as well as multispectral Sentinel 2A images. Three different forest test sites affected by fire in Bulgaria are examined. The results show that the classified thematic raster maps are distinguished by a good performance in monitoring the regrowth dynamics, with an average overall accuracy of 62.1% for all three test sites, ranging from 73.9% to 48.4% for the individual forests. [ABSTRACT FROM AUTHOR]

Stachys scardica Griseb. is a Balkan endemic species listed in The Red Data Book of Bulgaria with the conservation status "endangered". Successful micropropagation was achieved on MS medium supplemented with 1.5 mg/L benzyladenine (BA), followed by a subsequent ex vitro adaptation in an experimental field resulting in 92% regenerated plants. Using nuclear magnetic resonance (NMR), phenylethanoid glycosides (verbascoside, leucosceptoside A), phenolic acids (chlorogenic acid), iridoids (allobetonicoside and 8-OAc-harpagide), and alkaloids (trigonelline) were identified, characteristic of plants belonging to the genus Stachys. High antioxidant and radical scavenging activities were observed in both in situ and ex vitro acclimated S. scardica plants, correlating with the reported high concentrations of total phenols and flavonoids in these variants. Ex vitro adapted plants also exhibited a well-defined anti-inflammatory potential, demonstrating high inhibitory activity against the complement system. Employing a disk diffusion method, a 100% inhibition effect was achieved compared to positive antibiotic controls against Staphylococcus epidermidis and Propionibacterium acnes, with moderate activity against Bacillus cereus. The induced in vitro and ex vitro model systems can enable the conservation of S. scardica in nature and offer future opportunities for the targeted biosynthesis of valuable secondary metabolites, with potential applications in the pharmaceutical and cosmetic industries. [ABSTRACT FROM AUTHOR]

The growth of heterodox ideological configurations, or ideological inconsistencies, among the electorate of Western countries, has been offered as one explanation for recent momentous political events, such as Brexit or the election of Donald Trump as US President. Previous research, however, suggests that ideological inconsistency has been typical for Central and Eastern European (CEE) states for the past thirty years after the fall of the Socialist regimes there. Based on a survey of 102 active members of local and national party structures in Bulgaria, followed by in-depth interviews with the same respondents, I develop a conceptual and methodological approach aimed at objectively measuring Bulgarians' political values and ideological orientations. Building on previous research on the statistical independence of the social and economic dimensions of ideology, this study identifies three main models of ideological inconsistency at the party-elite level in Bulgaria, offering evidence of the "homogeneity in ideological inconsistency" in this post-Socialist country, with party elites and electorate following the same patterns of inconsistency. The existence of a conservative value complex, integrating traditionalist, statist, and nationalist attitudes regarding the social sphere, is another major finding of the study. I discuss the specific historical and socio-cultural background contributing to ideological inconsistency in Bulgaria and potential implications for the wider CEE region. [ABSTRACT FROM AUTHOR]

Simple Summary: Little is known about bat diversity in mountains above 2000 m a. s. l. in Europe. Here, we report data from a study carried out in the high-alpine karst landscape of Pirin Mountain, Bulgaria at an altitude ranging from 2300 to 2600 m a. s. l. Twenty bat species were detected using three inventory methods. Furthermore, we describe the highest bat swarming site in Europe, located at an altitude of 2600 m a. s. l. The study describes a pilot survey on bats in the highest areas of Pirin Mountain. The methods included examining subfossil bone remains, mist-netting, and recording echolocation calls. The study was conducted in August 2002 and 2013 and from 2019 to 2020. While in general, bat diversity tends to decrease with increasing altitude due to harsher environmental conditions, the present study, despite a short period, reveals high diversity. Twenty species, more than half of the Bulgarian bat fauna, were detected. The recording and analysis of vocal signatures proved to be the best way to inventory bat diversity. At least 13 species were detected by this method. Vespertilio murinus and Tadarida teniotis together make up more than 60% of all reliably determined echolocation sequences. Significant activity was found for Myotis myotis/blythii, Plecotus auritus, Eptesicus serotinus, and E. nilssonii. The registration of the latter species is of considerable faunistic interest. It was previously only known from a single specimen at one location in the country. The sex and age structure of the bat assemblage suggests that it is likely a swarming assemblage. The area is the highest swarming location in Europe. The results provide valuable information on bat ecology and behaviour, which can be used to inform management and protection efforts. [ABSTRACT FROM AUTHOR]