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Background/Objectives: Obesity and metabolic conditions increase the risk of metabolic-associated steatotic liver disease (MASLD). This study examined the risk of MASLD in 137 renal transplant recipients (RTRs) from a single-center hospital on the basis of their obesity and metabolic health status. Methods: Participants were categorized into four groups: metabolically healthy nonobese (MHNO), metabolically healthy obese (MHO), metabolically abnormal nonobese (MANO), and metabolically abnormal obese (MAO). MASLD was assessed using the hepatic steatosis index (HSI), calculated as 8 × (aspartate aminotransferase/alanine aminotransferase ratio) + body mass index + 2 (if diabetic) + 2 (if woman). The HSI scores were 29.50 ± 4.55, 38.08 ± 5.44, 33.61 ± 5.23, and 39.86 ± 4.13 in the MHNO, MHO, MANO, and MAO groups, respectively (p < 0.05). Results: Overall, 25.55% of the participants (57.14% men) were classified as having MASLD (HSI > 36). A multivariate-adjusted regression analysis revealed significantly higher HSI scores in the MAO group than in the MHNO group. Both MHO and MANO groups also had significantly higher HSI scores. The odds ratios for more severe MASLD were 2.74 (95% CI: 0.88–8.52) for the MANO group and 74.59 (95% CI: 13.29–418.68) for the MAO group compared with the MHNO group. Conclusions: These findings suggest that RTRs with obesity have a higher risk of MASLD, but even those with a normal weight and metabolic abnormalities are at increased risk. [ABSTRACT FROM AUTHOR]

Activating enhancer-binding protein 2 (AP-2) is a family of transcription factors (TFs) that play crucial roles in regulating embryonic and oncogenic development. In addition to splice isoforms, five major family members encoded by the TFAP2A/B/C/D/E genes have been identified in humans, i.e., AP-2α/β/γ/δ/ε. In general, the first three TFs have been studied more thoroughly than AP-2δ or AP-2ε. Currently, there is a relatively limited body of literature focusing on the AP-2 family in the context of gastroenterological research, and a comprehensive overview of the existing knowledge and recommendations for further research directions is lacking. Herein, we have collected available gastroenterological data on AP-2 TFs, discussed the latest medical applications of each family member, and proposed potential future directions. Research on AP-2 in gastrointestinal tumors has predominantly been focused on the two best-described family members, AP-2α and AP-2γ. Surprisingly, research in the past decade has highlighted the importance of AP-2ε in the drug resistance of gastric cancer (GC) and colorectal cancer (CRC). While numerous questions about gastroenterological disorders await elucidation, the available data undoubtedly open avenues for anti-cancer targeted therapy and overcoming chemotherapy resistance. In addition to gastrointestinal cancers, AP-2 family members (primarily AP-2β and marginally AP-2γ) have been associated with other health issues such as obesity, type 2 diabetes, liver dysfunction, and pseudo-obstruction. On the other hand, AP-2δ has been poorly investigated in gastroenterological disorders, necessitating further research to delineate its role. In conclusion, despite the limited attention given to AP-2 in gastroenterology research, pivotal functions of these transcription factors have started to emerge and warrant further exploration in the future. [ABSTRACT FROM AUTHOR]

Phytoncides and aromatherapy scents mainly derive from plant secondary metabolites and are now well known for their health benefits. In this study, we analyzed the chemical composition of the leaf-derived essential oil of Liquidambar formosana (Altingiaceae) using GC-MS; we also investigated the VOCs released from L. formosana leaves and the leaf essential oil at different temperatures by means of headspace gas chromatography–mass spectrometry (HS-GC-MS). Regarding the VOCs of the leaves, monoterpenes predominated the VOCs at both temperatures, mainly comprising sabinene, followed by γ-terpinene, α-terpinene, and α-pinene. The intensity of the leaf VOCs at 50 °C was nearly three times higher than that at 25 °C; the emission of monoterpenes significantly increases at higher environmental temperatures. The VOC emissions of oxygenated monoterpenes from the leaf essential oil increased at higher temperatures (50 °C), especially those of terpinen-4-ol. Our results reveal that HS-GC-MS can be used to conveniently and directly analyze the VOCs emitted from L. formosana leaves and their essential oils and to evaluate the influence of temperature on the composition of the VOCs of specimens. These VOC studies will assist in the sustainable development and utilization of L. formosana trees for forest therapy, as well as the use of their leaf essential oil for aromatherapy. [ABSTRACT FROM AUTHOR]

This study presents a predefined-time control strategy for rigid spacecraft, employing dynamic predictive techniques to achieve robust and precise attitude tracking within predefined time constraints. Advanced predictive algorithms are used to effectively mitigate system uncertainties and environmental disturbances. The main contributions of this work are introducing adaptive global optimization for period updates, which relaxes the original restrictive conditions; ensuring easier parameter adjustments in predefined-time control, providing a nonconservative upper bound on system stability; and developing a continuous, robust control law through terminal sliding mode control and predictive methods. Extensive simulations confirm the control scheme reduces attitude tracking errors to less than 0.01 degrees at steady state, demonstrating the effectiveness of the proposed control strategy. [ABSTRACT FROM AUTHOR]

Osmia excavata is an excellent pollinator in nature and plays a vital role in the conservation of agro-ecosystems and food security. Given the important role of the gut bacterial community in host health and regulation of host growth and development, using 16S rRNA gene sequencing data, the present study explored the composition of the gut bacterial community and its diversity at different life stages (eggs, young larvae, old larvae, young pupae, old pupae, and 1-day-old adults in cocoons) of the solitary bee Osmia excavata. The results showed that the core phyla in the gut of O. excavata at different life stages were Proteobacteria, Firmicutes, Bacteroidota, and Actinobacteriota, and the core genera were Sodalis, Tyzzerella, and Ralstonia. The highest intestinal bacterial diversity was found in the Egg period, and the lowest bacterial alpha diversity was found in the 1-day-old Adult period; the bacterial diversity of O. excavata showed a process of decreasing, as it was growing from the Egg period to the 1-day-old Adult period. Our study found that O. excavata undergoes a significant change in the structure of the gut flora when it grows from the young pupae to old pupae stage, a period of growth that coincides with the process of cocooning and isolation from the external environment after food depletion. This suggests that food and environmental factors are key contributors to the structure of the bacterial community in the gut of solitary bees. [ABSTRACT FROM AUTHOR]

The muscarinic acetylcholine receptor M3 (M3-mAChR) is involved in various physiological and pathological processes. Owing to specific cardioprotective effects, M3-mAChR is an ideal diagnostic and therapeutic biomarker for cardiovascular diseases (CVDs). Growing evidence has linked M3-mAChR to the development of multiple CVDs, in which it plays a role in cardiac protection such as anti-arrhythmia, anti-hypertrophy, and anti-fibrosis. This review summarizes M3-mAChR's expression patterns, functions, and underlying mechanisms of action in CVDs, especially in ischemia/reperfusion injury, cardiac hypertrophy, and heart failure, opening up a new research direction for the treatment of CVDs. [ABSTRACT FROM AUTHOR]

In the coal-rich provinces of Shanxi, Shaanxi, and Inner Mongolia, the landscape bears the scars of coal extraction—namely subsidence and deformation—that disrupt both the terrain and the delicate ecological balance. This research delves into the transformative journey these mining regions undergo, from pre-mining equilibrium, through the tumultuous phase of extraction, to the eventual restoration of stability post-reclamation. By harnessing a suite of analytical tools, including sophisticated remote sensing, UAV aerial surveys, and the meticulous ground-level sampling of flora and soil, the study meticulously measures the environmental toll of mining activities and charts the path to ecological restoration. The results are promising, indicating that the restoration initiatives are effectively healing the landscapes, with proactive interventions such as seeding, afforestation, and land rehabilitation proving vital in the swift ecological turnaround. Remote sensing technology, in particular, emerges as a robust ally in tracking ecological shifts, supporting sustainable practices and guiding ecological management strategies. This study offers a promising framework for assessing geological environmental shifts, which may guide policymakers in shaping the future of mining rehabilitation in arid and semi-arid regions. [ABSTRACT FROM AUTHOR]

Nonsense mutations are genetic mutations that create premature termination codons (PTCs), leading to truncated, defective proteins in diseases such as cystic fibrosis, neurofibromatosis type 1, Dravet syndrome, Hurler syndrome, Beta thalassemia, inherited bone marrow failure syndromes, Duchenne muscular dystrophy, and even cancer. These mutations can also trigger a cellular surveillance mechanism known as nonsense-mediated mRNA decay (NMD) that degrades the PTC-containing mRNA. The activation of NMD can attenuate the consequences of truncated, defective, and potentially toxic proteins in the cell. Since approximately 20% of all single-point mutations are disease-causing nonsense mutations, it is not surprising that this field has received significant attention, resulting in a remarkable advancement in recent years. In fact, since our last review on this topic, new examples of nonsense suppression approaches have been reported, namely new ways of promoting the translational readthrough of PTCs or inhibiting the NMD pathway. With this review, we update the state-of-the-art technologies in nonsense suppression, focusing on novel modalities with therapeutic potential, such as small molecules (readthrough agents, NMD inhibitors, and molecular glue degraders); antisense oligonucleotides; tRNA suppressors; ADAR-mediated RNA editing; targeted pseudouridylation; and gene/base editing. While these various modalities have significantly advanced in their development stage since our last review, each has advantages (e.g., ease of delivery and specificity) and disadvantages (manufacturing complexity and off-target effect potential), which we discuss here. [ABSTRACT FROM AUTHOR]

This retrospective study aimed to analyze the treatment effect and prognostic factors of pediatric acute myeloid leukemia (AML) patients with t(8;21). A total of 268 newly diagnosed pediatric AML (pAML) enrolled from 1 January 2005 to 31 December 2022 were retrospectively reviewed, and 50 (18.7%) patients harbored t(8;21) translocation. CR rate, OS, EFS, and RFS were assessed by multivariate Logistic and Cox regression models in these patients. Of the 50 patients, 2 patients abandoned treatment during the first induction course. Of the remaining 48 patients who received double-induction therapy and were included in the final analyses, CR1 and CR2 were 75.0% (36/48) and 95.8% (46/48), respectively. The overall three-year OS, EFS, and RFS were 68.4% (95% CI, 55.0–85.1), 64.2% (95% CI, 50.7–81.4), and 65.5% (95% CI, 51.9–82.8), respectively. The presence of loss of sex chromosome (LOS) at diagnosis (n = 21) was associated with a better 3-year OS [87.5% (95% CI, 72.7–100) vs. 52.7% (95% CI, 35.1–79.3), p = 0.0089], 3-year EFS [81.6% (95% CI, 64.7–100) vs. 49.7% (95% CI, 32.4–76.4), p = 0.023], and 3-year RFS [81.6% (95% CI, 64.7–100) vs. 51.7% (95% CI, 33.9–78.9), p = 0.036] than those without LOS (n = 27), and it was also an independent good prognostic factor of OS (HR, 0.08 [95% CI, 0.01–0.48], p = 0.005), EFS (HR, 0.22 [95% CI, 0.05–0.85], p = 0.029), and RFS (HR, 0.21 [95% CI, 0.05–0.90], p = 0.035). However, extramedullary leukemia (EML) featured the independent risk factors of inferior OS (HR, 10.99 [95% CI, 2.08–58.12], p = 0.005), EFS (HR, 4.75 [95% CI, 1.10–20.61], p = 0.037), and RFS (HR, 6.55 [95% CI, 1.40–30.63], p = 0.017) in pediatric individuals with t(8;21) AML. Further analysis of combining LOS with EML indicated that the EML+LOS− subgroup had significantly inferior OS (92.9%, [95% CI, 80.3–100]), EFS (86.2%, [95% CI, 70.0–100]), and RFS (86.2%, [95% CI, 80.3–100]) compared to the other three subgroups (all p < 0.001). LOS and EML are independent prognostic factors of OS, EFS, and RFS with t(8;21) pAML patients. LOS combined with EML may help improve risk stratification. [ABSTRACT FROM AUTHOR]

Dopamine (DA) and uric acid (UA) are essential for many physiological processes in the human body. Abnormal levels of DA and UA can lead to multiple diseases, such as Parkinson's disease and gout. In this work, a three-dimensional reduced graphene oxide–MXene (3D rGO-Ti3C2) composite electrode was prepared using a simple one-step hydrothermal reduction process, which could separate the oxidation potentials of DA and UA, enabling the simultaneous detection of DA and UA. The 3D rGO-Ti3C2 electrode exhibited excellent electrocatalytic activity towards both DA and UA. In 0.01 M PBS solution, the linear range of DA was 0.5–500 µM with a sensitivity of 0.74 µA·µM−1·cm−2 and a detection limit of 0.056 µM (S/N = 3), while the linear range of UA was 0.5–60 µM and 80–450 µM, with sensitivity of 2.96 and 0.81 µA·µM−1·cm−2, respectively, and a detection limit of 0.086 µM (S/N = 3). In 10% fetal bovine serum (FBS) solution, the linear range of DA was 0.5–500 µM with a sensitivity of 0.41 µA·µM−1·cm−2 and a detection limit of 0.091 µM (S/N = 3). The linear range of UA was 2–500 µM with a sensitivity of 0.11 µA·µM−1·cm−2 and a detection limit of 0.6 µM (S/N = 3). The modified electrode exhibited advantages such as high sensitivity, a strong anti-interference capability, and good repeatability. Furthermore, the modified electrode was successfully used for DA measurement in vivo. This could present a simple reliable route for neurotransmitter detection in neuroscience. [ABSTRACT FROM AUTHOR]

The intestinal epithelial barrier can prevent the invasion of pathogenic microorganisms and food antigens to maintain a consistent intestinal homeostasis. However, an imbalance in this barrier can result in various diseases, such as inflammatory bowel disease, malnutrition, and metabolic disease. Thus, the aim of this study was to select probiotic strains with epithelial barrier-enhancing ability in cell-based model and further investigate them for their improving effects on colitis mouse and weaned piglet models. The results showed that selected specific cell-free fermentation supernatants (CFSs) from Ligilactobacillus salivarius P1, Lactobacillus gasseri P12, and Limosilactobacillus reuteri G7 promoted intestinal epithelial cell growth and proliferation, strengthening the intestinal barrier in an intestinal epithelial cell line Caco-2 model. Further, the administration of CFSs of L. salivarius P1, L. gasseri P12, and L. reuteri G7 were found to ameliorate DSS-induced colitis in mice. Additionally, spray-dried powders of CFS from the three strains were examined in a weaned piglet model, only CFS powder of L. reuteri G7 could ameliorate the feed/gain ratio and serum levels of D-lactate and endotoxin. In conclusion, a new potential probiotic strain, L. reuteri G7, was selected and showed ameliorating effects in both colitis mouse and weaned piglet models. [ABSTRACT FROM AUTHOR]

To meet the environmental protection and flame retardancy requirements for epoxy resins (EPs) in certain fields, in this study, a novel triazine-ring-containing DOPO-derived compound (VDPD), derived from vanillin, 2,4-Diamino-6-phenyl-1,3,5-triazine, and 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO), was synthesized using a one-pot method. Flame-retardant epoxy resin (FREP) was prepared by adding various ratios of VDPD to EP and curing with 4,4-diaminodiphenylmethane (DDM). The curing behavior, thermal stability, mechanical properties, and flame-retardant properties of the FREP were examined in various tests. According to the results, when the amount of VDPD added to the EP increased, the glass transition temperature of the FREP decreased linearly, and the flame-retardant properties gradually improved. With a 0.4 wt.% P content, the vertical burning rating of EP/DDM/VDPD-0.4 (according to the theoretical content of VDPD) reached the V-0 level, and the LOI value reached 33.1%. In addition, the results of a CCT showed that the peak heat release rate (PHRR) of EP/DDM/VDPD-0.4 decreased by 32% in comparison with that of the EP. Furthermore, compared with those of the EP, the tensile strength of EP/DDM/VDPD-0.4 decreased from 80.2 MPa to 74.3 MPa, only decreasing by 6 MPa, and the tensile modulus increased. Overall, VDPD can maintain the mechanical properties of EP and effectively improve its flame-retardant properties. [ABSTRACT FROM AUTHOR]

Simple Summary: Osmia excavata Alfken is an excellent pollinator. A variety of factors affect the rate of O. excavata release from its cocoon. However, rapid improvement of the concentrated decocooning of O. excavata has not been sufficiently investigated. In this study, we examined the effect of water immersion on the decocooning rate of O. excavata. Our results showed that water immersion significantly improved O. excavata decocooning. This will provide technical support to improve effective application of O. excavata. Illumina Novaseq™ 6000 transcriptome sequencing showed muscle-related functions play important roles in O. excavata decocooning in response to water immersion. The timing of decocooning and nesting during the flowering period are crucial for the reproduction and pollination activities of Osmia excavata. In order to improve the pollination efficiency of O. excavata, it is crucial to find a way to break the cocoon quickly. Our results showed that the decocooning rates at 6, 12, 24, 36, 48, and 72 h after 30 min of water immersion (WI) were 28.67%, 37.33%, 37.33%, 41.33%, 44.33%, and 53.00%, respectively. The decocooning rate fold of 6 h was 14.33 compared with the control group. Transcriptome sequencing resulted in 273 differentially expressed genes (DEGs) being identified between the WI and control groups. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that muscle-related functions play important roles in O. excavata decocooning in response to WI. Cluster analysis also showed that DEGs in cardiac muscle contraction and adrenergic signaling in cardiomyocytes were up-regulated in response to WI-promoted decocooning. In conclusion, the rate of decocooning can be improved by WI in a short time. During WI-promoted decocooning, muscle-related pathways play an important role. Therefore, the application of this technology will improve the pollination effect of O. excavata. [ABSTRACT FROM AUTHOR]

The higher the atmosphere is, the larger the deviations in atmospheric temperature and humidity are between the vertical column atmosphere above the cross-section of a satellite instrument and a ray's trajectory from the cross-section to the satellite. In general, satellite instruments that observe using cross-orbit scanning result in the difference between the observed radiance and the simulations using this method becoming incrementally larger and larger as the cross-section moves to the edge of the satellite's orbit. The deviations depend on the distance from the column to the ray trajectory and on the horizontal gradient of variables in the distance. In fact, the horizontal gradient of water vapour is larger than the gradient of temperature in clear scenarios, which could introduce an impact of temperature and water vapour on the simulated radiance of a satellite. In this study, a new method to simulate upgoing and downgoing radiation synchronously was developed, using the observing path tracking method. The conventional vertical initial atmospheric profile (Exp.1) and the profiles along the upgoing and downgoing rays of the satellite's observation (Exp.2) were established, in order to simulate the observed radiance of MWHS-II of FY-3D using global numerical forecasts with resolutions of 15 km and 25 km. The results showed that, for channels in the oxygen and water vapour absorption line on the microwave spectrum, deviations of the two atmospheric profiles were larger at the scan edge (0.01 K) than those at the nadir (0.001 K), and were larger in the upper atmosphere than in the lower atmosphere. The deviation was usually negative in low-latitude regions and was positive in southern high-latitude regions. Such results were obtained in experiments using both the numerical forecast method with 15 km grids and the forecast method with 25 km grids. Deviations were analysed for representative channels at 118 GHz and 183 GHz. Then, the results indicated that bigger deviations between the two experiments were observed in the water vapour absorption line than in the oxygen absorption line in the microwave spectrum. In conclusion, this indicates that, because of the greater horizontal gradient of water vapour, the stronger localisation of water vapour makes the atmospheric profile along the satellite's observing ray have more increments in the simulated radiance at the scan edge, compared to the atmospheric column profile. [ABSTRACT FROM AUTHOR]

Global warming may increase potential evapotranspiration (ETp), reducing the water resources in Yangzi River Delta. Therefore, it is important to investigate the trend of ETp there under the background of climate change. To this purpose, the systematic biases in temperature outputs of 24 global climate models (GCMs) under 3 shared socioeconomic pathways—representative concentration pathways (SSPs) emission scenarios (SSP1-2.6, SSP2-4.5, and SSP5-8.5)—are first corrected by using 8 bias correction methods. Then, the trend of ETp in Yangtze River Delta is projected by using 4 ETp calculation formulas (Blaney–Criddle, Hargreaves–Samani, Makkink, and Priestley–Taylor). The uncertainty of the projections is estimated and decomposed by using multi-way analysis of variance frameworks. The influence of uncertainty on the projected change signal is quantified by using the signal-to-noise ratio. The results show that all emission scenarios indicate robust increments of ETp. Specifically, relative to 1971~2000, ETp will increase by 0.14~0.17 mm d−1 (5.7~6.8%) during 2021~2050 and by 0.21~0.41 mm d−1 (8.5~16.7%) during 2061~2090, respectively. During 2021~2050, the uncertainty of ETp projections is dominantly contributed by the main effects of GCM (63%) and the ETp calculation formula (24%). During 2061~2090, it is mainly contributed by the main effect of GCM (36%), followed by the main effects of the emission scenario (34%) and the ETp calculation formula (18%). The ETp projections are generally reliable and robust during the two projection periods. [ABSTRACT FROM AUTHOR]

Machine- and deep-learning methods are used for industrial applications in prognostics and health management (PHM) for semiconductor processing and equipment anomaly detection to achieve proactive equipment maintenance and prevent process interruptions or equipment downtime. This study proposes a Pruning Quantized Unsupervised Meta-learning DegradingNet Solution (PQUM-DNS) for the fast training and retraining of new equipment or processes with limited data for anomaly detection and the prediction of various equipment and process conditions. This study utilizes real data from a factory chiller host motor, the Paderborn current and vibration open dataset, and the SECOM semiconductor open dataset to conduct experimental simulations, calculate the average value, and obtain the results. Compared to conventional deep autoencoders, PQUM-DNS reduces the average data volume required for rapid training and retraining by about 75% with similar AUC. The average RMSE of the predictive degradation degree is 0.037 for Holt–Winters, and the model size is reduced by about 60% through pruning and quantization which can be realized by edge devices, such as Raspberry Pi. This makes the proposed PQUM-DNS very suitable for intelligent equipment management and maintenance in industrial applications. [ABSTRACT FROM AUTHOR]

Flowers of Paulownia spp. wither and fall on a large scale after blooming in spring and have potential allelopathic effects on surrounding plants, including crops and weeds. In this study, the phytotoxic effects of water extracts of Paulownia tomentosa flower litter (EPF) on wheat (Triticum aestivum L.), lettuce (Lactuca sativa L.), green bristlegrass (Setaria viridis L.) and purslane (Portulaca oleracea L.) were evaluated in the laboratory. The mode of action of the phytotoxicity of EPF on lettuce seedlings was studied and the secondary metabolites in EPF were analyzed by liquid chromatography high-resolution mass spectrometry (LC-HRMS). The results show that EPF significantly inhibited the seed germination and seedling growth of four target plants in a concentration-dependent manner. In addition, EPF could induce the excessive accumulation of reactive oxygen species (ROS) flowing with oxidative damage of the lipid bilayer of the biofilm, resulting in reduced cell viability and even apoptosis in lettuce. There were 66 secondary metabolites identified by LC-HRMS in P. tomentosa flowers. Among them, 10 compounds, including salicylic acid, caffeic acid, parthenolide, 7-hydroxycoumarin and abscisic acid (ABA), were all known allelochemicals. In summary, P. tomentosa flower litter displayed significant allelopathic effects, which were related to the accumulation of ROS in target plants. Phenolic acids, flavonoids as well as ABA are probably the main allelochemicals of P. tomentosa flowers. [ABSTRACT FROM AUTHOR]

Engineered nano-ZnO is extensively utilized in both production and daily life, leading to its inevitable entry into the wastewater treatment system through various pathways. Nitrogen removal microorganisms in wastewater treatment systems are highly susceptible to environmental impacts. The antibacterial properties of nano-ZnO can impede the biological nitrogen removal (BNR) process and adversely affect the nitrogen removal performance. A comprehensive understanding of the inhibitory effect and mechanism of nano-ZnO on the BNR process is crucial in devising appropriate countermeasures to ensure optimal nitrogen removal performance. This review provides an overview of the sources of nano-ZnO in the environment, its impact on the BNR process, and the inhibition mechanism, and proposes potential methods that can mitigate the inhibitory effect of nano-ZnO. Additionally, future prospects are also discussed. This review serves as a foundation for a deeper understanding of the inhibition of engineered nano-ZnO on the BNR process and aids in guiding efforts to maintain the nitrogen removal performance in the presence of engineered nano-ZnO. [ABSTRACT FROM AUTHOR]

Reducing carbon emissions is of paramount importance to the accomplishment of the 2030 Sustainable Development Goals. The effect of rural e-commerce on agricultural carbon emissions (ACEs) is controversial, and particularly the mechanism behind the effect is unknown. To identify the impact of rural e-commerce on agricultural carbon emissions and its mechanisms, we take advantage of China's Rural E-Commerce Demonstration County Program (REDCP) as a quasi-natural experiment and use the multi-period difference-in-difference (DID) model to investigate the relationship between rural e-commerce and agricultural carbon emissions. Our data are based on panel data of 355 prefecture-level cities from 2010 to 2019 in China. We identify that rural e-commerce can reduce agricultural carbon emissions by an average of 14.4%, but this effect is not long-lasting. Mechanism analyses suggest that the reduction effect of rural e-commerce on agricultural carbon emissions is mainly due to fostering agricultural economic growth, increasing the share of low-carbon industry, and improving agricultural total factor productivity (TFP). Further heterogeneity analyses demonstrate that rural e-commerce has better carbon emissions reduction performance in eastern cities as well as in non-major grain-producing cities in China. [ABSTRACT FROM AUTHOR]

Background: Sichuan taimen ( Hucho bleekeri ) is a fish species endemic to China's upper Yangtze River drainage and has significant value as an aquatic resource. It was listed as a first-class state-protected wild animal by the Chinese government due to its very limited distribution and wild population at present., Methods: To elucidate the diversity of microorganisms in rearing water environments for H. bleekeri , metagenomic sequencing was applied to water samples from the Maerkang and Jiguanshan fish farms, where H. bleekeri were reared., Results: The results revealed that Pseudomonadota was the dominant phylum in the microbial communities of the water samples. Among the shared bacterial groups, Cyanobacteriota, Actinomycetota, Planctomycetota, Nitrospirota, and Verrucomicrobiota were significantly enriched in the water environment of Jiguanshan ( p < 0.01), while Bacteroidota was more enriched in that of Maerkang ( p < 0.01). Additionally, the Shannon diversity and Simpson index of the microbial community in the water environment of Maerkang were lower than in that of Jiguanshan., Conclusions: The present study demonstrated the similarities and differences in the microbial compositions of rearing water environments for H. bleekeri , which are expected to benefit the artificial breeding of H. bleekeri in the future.