1. Long Non-Coding Ribonucleic Acid PGM5-AS1 Regulates Lung Cancer Progression by Modulating microrna-587 to Affect Cell Ferroptosis.
- Author
-
Jian Zhong, Jie Jiang, Lei Cao, Zicheng Liu, and Bo Wang
- Subjects
- *
RNA physiology , *DISEASE progression , *REVERSE transcriptase polymerase chain reaction , *FLOW cytometry , *WESTERN immunoblotting , *LUNG tumors , *DESCRIPTIVE statistics , *CELL lines , *DATA analysis software , *CELL death - Abstract
The objective of this study was to analyze the influence of long non-coding ribonucleic acid PGM5-AS1 on lung cancer cell ferroptosis through microrna-587. To this end, the research team has included the abnormal expression vectors of PGM5-AS1 and microrna-587 were transfected into human lung cancer cell line A549 to detect alterations in cell activity, apoptosis, cell cycle, oxidative stress, iron ion and ferroptosis-associated proteins. The results show silencing PGM5-AS1 or elevating microrna-587 promoted A549 proliferation and invasion, while inhibiting apoptosis and resisting ferroptosis. However, increasing PGM5-AS1 or silencing microrna-587 enhanced A549 apoptosis and induced ferroptosis (P < 0.05). And in the salvage experiment, the research team found that microrna-587 mimics inhibited PGM5-AS1-wild sequence fluorescence activity. The biological behavior of A549 after increasing (or silencing) PGM5-AS1 and microrna-587 was not different from that of cells transfected with microrna-587 negative control (P > 0.05). These results suggest that lowexpressed PGM5-AS1 can promote lung cancer cell viability, inhibit apoptosis and resist ferroptosis through targeted increase of microrna-587. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF