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In this study, a new type of multi-arch tunnel construction method is proposed. This effort is undertaken due to the many disadvantages of the traditional multi-arch tunnel construction method. Furthermore, this method omits the construction of a middle pilot tunnel, and it has the advantages of safety, high efficiency, and being economical. When using the method of continuous arch tunneling without a middle pilot tunnel, the blasting of the first tunnel and the following tunnel has a greater impact on the surrounding rock damage, as well as on the supporting structure of the same cross-section. Therefore, this study uses LS-DYNA finite element software to construct a three-dimensional numerical model. In addition, the perimeter rock damage law and mechanical response characteristics of the supporting structure in the same cross-section of the first tunnel, as well as the following tunnel after blasting without a middle pilot tunnel, are analyzed. At the same time, the results of the study are based on optimizing the blasting program, and these are then applied to the field. Through the results, it is found that, after blasting a continuous arch tunnel without a medial pilot tunnel, the surrounding rock damage in the arch cross-region of the double-arch tunnel (where the arch top and the arch shoulder are more significant) and the effective stress of the supporting structure exceed the strength design value. In addition, the maximum adequate pressure is distributed in the medial diaphragm wall. With the optimized blasting scheme, the range of the peripheral rock damage is reduced by a maximum of 67%, and the effective stress in the supporting structure is reduced by 25.9–64.8%. The research results are of great significance in terms of improving construction safety, economic efficiency, and project quality, as well as in promoting the research and development of new work methods for double-arch tunnels. [ABSTRACT FROM AUTHOR]

The rapid and precise monitoring of peripheral blood miRNA levels holds paramount importance for disease diagnosis and treatment monitoring. In this study, we propose an innovative research strategy that combines the catalytic hairpin assembly reaction with SERS signal congregation and enhancement. This combination can significantly enhance the stability of SERS detection, enabling stable and efficient detection of miRNA. Specifically, our paper-based SERS detection platform incorporates a streptavidin-modified substrate, biotin-labeled catalytic hairpin assembly reaction probes, 4-ATP, and primer-co-modified gold nanoparticles. In the presence of miRNA, the 4-ATP and primer-co-modified gold nanoparticles can specifically recognize the miRNA and interact with the biotin-labeled CHA probes to initiate an interfacial catalytic hairpin assembly reaction. This enzyme-free high-efficiency catalytic process can accumulate a large amount of biotin on the gold nanoparticles, which then bind to the streptavidin on the substrate with the assistance of the driving liquid, forming red gold nanoparticle stripes. These provide a multitude of hotspots for SERS, enabling enhanced signal detection. This innovative design achieves a low detection limit of 3.47 fM while maintaining excellent stability and repeatability. This conceptually innovative detection platform offers new technological possibilities and solutions for clinical miRNA detection. [ABSTRACT FROM AUTHOR]

Studies of the mechanical response behavior of slate and the establishment of corresponding damage-ontological relationships are crucial for improving safety and avoiding disasters in construction projects. For the study reported in this paper, we first assumed that slate is a transverse isotropic body. Next, to characterize disparities in elastic characteristics between axial and radial orientations, we introduced five distinct elastic parameters for these two directions. Specifically, these parameters were Young’s modulus E1, Poisson’s coefficient v1 (associated with parallel bedding planes), Young’s modulus E2, Poisson’s coefficient v2, and shear modulus G2 (associated with perpendicular bedding planes). We then established a statistical damage-evolution equation for transverse isotropic slate based on a lognormal distribution, and we constructed a statistical damage-constitutive model for laminated slate under three-way stress by considering the shear–slip deformation and closure deformation of the laminated surface. Finally, we demonstrated the effectiveness of our model by comparing its output data with results obtained in triaxial compression tests on slate. We found that the differential stress–strain curves obtained from the model and the tests were in good agreement in the peak front. Average relative errors of 15.62% and 16.19% were recorded for cases of 5 Mpa and 10 Mpa of enclosing pressure, respectively. The rationality of the established transverse isotropic slate damage-constitutive relationship was therefore proved. [ABSTRACT FROM AUTHOR]