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In recent years, several studies have undergone automatic blood vessel segmentation based on unsupervised and supervised algorithms to reduce user interruption. Deep learning networks have been used to get highly accurate segmentation results. However, the incorrect segmentation of pathological information and low micro-vascular segmentation is considered the challenges present in the existing methods for segmenting the retinal blood vessel. It also affects different degrees of vessel thickness, contextual feature fusion in technique, and perception of details. A deep learning-aided method has been presented to address these challenges in this paper. In the first phase, the preprocessing is performed using the retinal fundus images employed by the black ring removal, LAB conversion, CLAHE-based contrast enhancement, and grayscale image. Thus, the blood vessel segmentation is performed by a new deep learning model termed optimized ResUNet + +. As an improvement to this deep learning architecture, the activation function is optimized by the J-AGSO algorithm. The objective function for the optimized ResUNet + + -based blood vessel segmentation is to minimize the binary cross-entropy loss function. Further, the post-processing of the images is carried out by median filtering and binary thresholding. By verifying the standard benchmark datasets, the proposed model outperforms and attains enhanced performance. [ABSTRACT FROM AUTHOR]