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The self-potential method (SP) has been used extensively to reveal some model parameters of various ore deposits. However, estimating these parameters can be challenging due to the mathematical nature of the inversion process. To address this issue, we propose here a novel global optimizer called the Modified Barnacles Mating Optimizer (MBMO). We improved upon the original approach by incorporating a variable genital length strategy, a novel barnacle offspring evolving method, and an out-of-bounds correction approach. The MBMO has not been previously applied to geophysical anomalies. Prior to inversion of real data sets, modal and sensitivity Analyzes were conducted using a theoretical model with multiple sources. The Analyzes revealed that the problem is modal in nature, model parameters have varying levels of sensitivity, and an algorithm that can well balance global exploration with local exploitation is required to solve this problem. The MBMO was tested on theoretical SP anomalies and four real datasets from Türkiye, Canada, India, and Germany. Its performance was compared to the original version under equal conditions. Uncertainty determination studies were carried out to comprehend the reliability of the solutions obtained via both algorithms. The findings indicated clearly that the MBMO outperformed its original version in estimating the model parameters from SP anomalies. The modifications presented here improved its ability to search for the global minimum effectively. In addition to geophysical datasets, experiments with 11 challenging benchmark functions demonstrated the advantages of MBMO in optimization problems. Theoretical and field data applications showed that the proposed algorithm can be used effectively in model parameter estimations from SP anomalies of ore deposits with the help of total gradient anomalies. [ABSTRACT FROM AUTHOR]