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[Display omitted] • Sn-1-lysophosphatidylcholine underwent acyl migration due to structural instability. • Acyl migration rate was higher under acidic or alkaline conditions. • Nitrogen/vacuum increased yield of conjugated linoleic acid lysophosphatidylcholine. • The maximum yield was achieved when the vacuum pressure was 13.3 KPa. Sn -Glycerol-3 - phosphatidylcholine (GPC) was prepared by hydrolysis of phosphatidylcholine (PC) catalyzed by phospholipase A 1 (PLA 1). Nitrogen flow assisted the esterification of conjugated linoleic acid (CLA) and GPC to produce conjugated linoleic acid lysophosphatidylcholine (LPC − CLA). The effects of different reaction conditions on the PC conversion and acyl migration rates were investigated, and the acyl migration mechanism under acidic and alkaline conditions was studied. In addition, the optimum conditions for the esterification of CLA and GPC were selected. The optimal condition for the hydrolysis of PC was an enzyme loading of 5 %, pH of 5, reaction temperature of 50 ℃, and reaction time of 3 h. The results also showed that the maximum esterification rate reached 82.37 % at an enzyme loading of 15 %, CLA/GPC molar ratio of 50:1, and vacuum pressure of 13.3 kPa. This study not only improved the bioavailability of PC but also effectively increased the content of LPC − CLA. [ABSTRACT FROM AUTHOR]

Oils are susceptible to oxidation, so encapsulation of them with small molecule gels and proteins through dynamic high-pressure microfluidization is an effective method of protection. In this study, conjugated linoleic acid (CLA)-rich oil-in-water oleogel emulsions (CLA-Og/W) was prepared by dynamic high-pressure microfluidization. By results, when dynamic high-pressure microfluidization pressure was 80 MPa, zeta potential of CLA-Og/W was −38 mV, the droplet size decreased to 91.3 nm. The surface roughness of the CLA-Og/W reduced to a minimum of 2.75 nm at 80 MPa. The surface tension decreased to 38.36 mN/m. The rheological properties showed that the microfluidized CLA-Og/W had favorable viscoelasticity and increased apparent viscosity. In addition, the fatty acid composition of CLA-Og/W did not change significantly compared to CLA-safflower seed oil. After 14 d of storage at 60 °C, the CLA content of CLA-Og/W was 40.19%. Consequently, CLA-Og/W treated with dynamic high-pressure microfluidization had more stable physicochemical properties and better retention of CLA. • Oleogel emulsion was prepared by dynamic high-pressure microfluidization. • Minimum droplet size obtained at 80 MPa processing pressure. • After 14 d of storage at 60 °C, the conjugated linoleic acid was 40.19%. [ABSTRACT FROM AUTHOR]